公开(公告)号：US08420311B2

公开(公告)日：2013-04-16

申请号：US12298808

申请日：2007-04-26

申请人： Vivian Huerta Galindo ,  Glay Chinea Santiago ,  Noralvis Fleitas Salazar ,  Alejandro Miguel Martin Dunn ,  Monica Sarria Nunez ,  Osmany Guirola Cruz ,  Patricia Gabriela Toledo Mayora ,  Aniel Sanchez Puente ,  Vladimir Armando Besada Perez ,  Osvaldo Reyes Acosta ,  Hilda Elisa Garay Perez ,  Ania Cabrales Rico ,  Alexis Musacchio Lasa ,  Gabriel Ramon Padron Palomares ,  Luis Javier Gonzalez Lopez

发明人： Vivian Huerta Galindo ,  Glay Chinea Santiago ,  Noralvis Fleitas Salazar ,  Alejandro Miguel Martin Dunn ,  Monica Sarria Nunez ,  Osmany Guirola Cruz ,  Patricia Gabriela Toledo Mayora ,  Aniel Sanchez Puente ,  Vladimir Armando Besada Perez ,  Osvaldo Reyes Acosta ,  Hilda Elisa Garay Perez ,  Ania Cabrales Rico ,  Alexis Musacchio Lasa ,  Gabriel Ramon Padron Palomares ,  Luis Javier Gonzalez Lopez

IPC分类号： C12Q1/70 ,  A61K39/12

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C07K14/70596 ,  C07K14/8107 ,  C12N2770/24122 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/39 ,  Y02A50/394 ,  Y02A50/396

摘要： The present invention is related to a method for blocking the infection of cells by dengue virus, based on interfering the direct interaction of the viral envelope protein with a cellular receptor or its indirect interaction with said cellular receptor through a carrier protein, as well as related uses; wherein said cellular receptor is the alpha-2 macroglobulin receptor, also known as the low density receptor-related protein or as CD91, and said carrier protein is human alpha-2 macroglobulin.

摘要翻译： 本发明涉及一种基于干扰病毒包膜蛋白与细胞受体的直接相互作用或其通过载体蛋白与所述细胞受体的间接相互作用而阻断登革热病毒感染细胞的方法，以及相关的 用途; 其中所述细胞受体是α-2巨球蛋白受体，也称为低密度受体相关蛋白或CD91，所述载体蛋白是人α-2巨球蛋白。

公开(公告)号：US20100273702A1

公开(公告)日：2010-10-28

申请号：US12513085

申请日：2007-10-30

申请人： Glay Chinea Santiago ,  Vivian Juerta Galindo ,  Alejandro Miguel Martin Dunn ,  Noralvis Fleitas Salazar ,  Osmany Guirola Cruz ,  Patricia Gabriela Toledo Mayora ,  Mónica Srria Nüñez ,  Alexis Musacchio Lasa ,  Osvaldo Reyes Acosta ,  Hilda Elisa Garay Pérez ,  Ania Cabrales Rico

发明人： Glay Chinea Santiago ,  Vivian Juerta Galindo ,  Alejandro Miguel Martin Dunn ,  Noralvis Fleitas Salazar ,  Osmany Guirola Cruz ,  Patricia Gabriela Toledo Mayora ,  Mónica Srria Nüñez ,  Alexis Musacchio Lasa ,  Osvaldo Reyes Acosta ,  Hilda Elisa Garay Pérez ,  Ania Cabrales Rico

IPC分类号： A61K38/16 ,  C07K14/00 ,  A61P31/14

CPC分类号： A61K47/48315 ,  A61K38/00 ,  A61K47/645 ,  C07K14/005 ,  C07K2319/04 ,  C07K2319/10 ,  C12N2770/24022 ,  C12N2770/24122 ,  C12N2770/24222 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/389 ,  Y02A50/393 ,  Y02A50/395

摘要： The present invention is relative to chimerical peptides, whose primary structure holds at least one segment which inhibits the activation of the NS3 protease of a virus from the Flaviviridae family, they also contain a cell penetrating segment and they are capable of inhibiting or attenuate the viral infection. This invention is also relative to pharmaceutical compounds which contain these chimerical peptides for the prevention and/or treatment of the infection caused by a virus of the Flaviviridae family.

摘要翻译： 本发明是相对于嵌合肽，其主要结构保持至少一个片段，其抑制来自黄病毒科的病毒的NS3蛋白酶的活化，它们还含有细胞穿透片段，并且它们能够抑制或减弱病毒 感染。 本发明还涉及含有这些嵌合肽用于预防和/或治疗由黄病毒科病毒引起的感染的药物化合物。

公开(公告)号：US20090312190A1

公开(公告)日：2009-12-17

申请号：US12094503

申请日：2006-11-21

申请人： Glay Chinea Santiago ,  Vivian Huerta Galindo ,  Alejandro Miguel Martin Dunn ,  Jorge Victor Gavilondo Cowley ,  Noralvis Fleitas Salazar ,  Osmany Guirola Cruz ,  Jeovanis Gil Valdés ,  Aída Zulueta Morales ,  Lisset Hermida Cruz ,  Marta Ayala Avila ,  Diamilé Gonzáles Roche ,  Rolando Paez Meireles ,  Patricia Gabriela Toledo Mayora ,  Mónica Sarria Núnez ,  Alexis Musacchio Lasa ,  Yuliet Mazola Reyes

发明人： Glay Chinea Santiago ,  Vivian Huerta Galindo ,  Alejandro Miguel Martin Dunn ,  Jorge Victor Gavilondo Cowley ,  Noralvis Fleitas Salazar ,  Osmany Guirola Cruz ,  Jeovanis Gil Valdés ,  Aída Zulueta Morales ,  Lisset Hermida Cruz ,  Marta Ayala Avila ,  Diamilé Gonzáles Roche ,  Rolando Paez Meireles ,  Patricia Gabriela Toledo Mayora ,  Mónica Sarria Núnez ,  Alexis Musacchio Lasa ,  Yuliet Mazola Reyes

IPC分类号： C40B30/02 ,  A61K39/00 ,  A61K39/12 ,  C12N7/00 ,  C12N5/00 ,  C40B30/04 ,  C40B40/10 ,  C07K16/00 ,  C07H21/04 ,  A61K39/02

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/55566 ,  C07K2319/02 ,  C07K2319/21 ,  C12N2770/24022 ,  C12N2770/24122 ,  C12N2770/24134 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/39 ,  Y02A50/394 ,  Y02A50/396

摘要： The present invention is related to the field of the pharmaceutical industry, and describes a conserved area on the surface of the E protein that can be used for the development of wide-spectrum antiviral molecules to be employed in the prophylaxis and/or treatment of infections due to Dengue Virus serotypes 1-4 and other flaviviruses. The invention also covers chimeric proteins to be used as vaccines or as a prophylactic or therapeutic treatment against the four serotypes of Dengue Virus and other flaviviruses.

摘要翻译： 本发明涉及制药工业的领域，并且描述了可用于开发用于预防和/或治疗感染的广谱抗病毒分子的E蛋白表面上的保守区域 由于登革热病毒血清型1-4和其他黄病毒。 本发明还涵盖用作疫苗的嵌合蛋白或针对登革热病毒和其他黄病毒的四种血清型的预防或治疗性治疗。

公开(公告)号：US08674063B2

公开(公告)日：2014-03-18

申请号：US12513085

申请日：2007-10-30

申请人： Glay Chinea Santiago ,  Vivian Huerta Galindo ,  Alejandro Miguel Martin Dunn ,  Noralvis Fleitas Salazar ,  Osmany Guirola Cruz ,  Patricia Gabriela Toledo Mayora ,  Mónica Sarría Núñez ,  Alexis Musacchio Lasa ,  Olvaldo Reyes Acosta ,  Hilda Elisa Garay Pérez ,  Anìa Cabrales Rico

发明人： Glay Chinea Santiago ,  Vivian Huerta Galindo ,  Alejandro Miguel Martin Dunn ,  Noralvis Fleitas Salazar ,  Osmany Guirola Cruz ,  Patricia Gabriela Toledo Mayora ,  Mónica Sarría Núñez ,  Alexis Musacchio Lasa ,  Olvaldo Reyes Acosta ,  Hilda Elisa Garay Pérez ,  Anìa Cabrales Rico

IPC分类号： C07K14/00

CPC分类号： A61K47/48315 ,  A61K38/00 ,  A61K47/645 ,  C07K14/005 ,  C07K2319/04 ,  C07K2319/10 ,  C12N2770/24022 ,  C12N2770/24122 ,  C12N2770/24222 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/389 ,  Y02A50/393 ,  Y02A50/395

摘要： The present invention is relative to chimerical peptides, whose primary structure holds at least one segment which inhibits the activation of the NS3 protease of a virus from the Flaviviridae family, they also contain a cell penetrating segment and they are capable of inhibiting or attenuate the viral infection. This invention is also relative to pharmaceutical compounds which contain these chimerical peptides for the prevention and/or treatment of the infection caused by a virus of the Flaviviridae family.

摘要翻译： 本发明是相对于嵌合肽，其主要结构保持至少一个片段，其抑制来自黄病毒科的病毒的NS3蛋白酶的活化，它们还含有细胞穿透片段，并且它们能够抑制或减弱病毒 感染。 本发明还涉及含有这些嵌合肽用于预防和/或治疗由黄病毒科病毒引起的感染的药物化合物。

公开(公告)号：US6146635A

公开(公告)日：2000-11-14

申请号：US930917

申请日：1997-09-16

申请人： Carlos Antonio Durate Cano ,  Enrique Gerardo Guillen Nieto ,  Anabel Alvarez Acosta ,  Luis Emilio Carpio Munoz ,  Diogenes Quintana Vazquez ,  Carmen Elena Gomez Rodriquez ,  Recardo de la Caridid Siva Rodriguez ,  Consuelo Nazabal Galvez ,  Maria De Jesus Leal Angulo ,  Alejandro Miguel Martin Dunn

发明人： Carlos Antonio Durate Cano ,  Enrique Gerardo Guillen Nieto ,  Anabel Alvarez Acosta ,  Luis Emilio Carpio Munoz ,  Diogenes Quintana Vazquez ,  Carmen Elena Gomez Rodriquez ,  Recardo de la Caridid Siva Rodriguez ,  Consuelo Nazabal Galvez ,  Maria De Jesus Leal Angulo ,  Alejandro Miguel Martin Dunn

IPC分类号： G01N33/569 ,  A61K39/00 ,  A61K39/095 ,  A61K39/395 ,  A61K39/40 ,  A61P31/04 ,  A61P31/12 ,  C07K1/22 ,  C07K14/16 ,  C07K14/22 ,  C07K16/00 ,  C07K16/12 ,  C07K19/00 ,  C12N1/21 ,  C12N15/02 ,  C12N15/31 ,  C12N15/48 ,  C12N15/62 ,  C12N15/70 ,  C12P21/02 ,  C12P21/08 ,  C12R1/19 ,  A61K39/02 ,  A61K39/21

CPC分类号： C07K14/005 ,  C07K14/22 ,  C07K16/1217 ,  C12N15/62 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/75 ,  C12N2740/16122 ,  Y10S530/82 ,  Y10S530/825

摘要： The present invention relates to biotechnology and genetic engineering, particularly the expression of proteins of viral origin in microorganisms through their fusion, by applying the recombinant DNA technology, to bacterial peptides. The present invention provides an efficient process for the expression in Escherichia coli of heterologous proteins as fusion polypeptides with a view to obtaining them with a high degree of purity, in commercially useful amounts, and in an appropriate form for their inclusion in vaccine preparations intended to human use. To this effect, what is essentially used is a stabilizing sequence derived from the first 47 amino acids of the antigen P64k of Neisseria meningitidis B:4:P1.15. In particular, use is made of a recombinant plasmid containing said sequence, under the control of the tryptophane promotor of E. coli and of the terminator of the transcription of the phage T4, including restriction sites which provide for the cloning in phase of DNA fragments coding for polypeptides of interest. The process of the invention is applicable to the pharmaceutical industry, for the development of diagnostic systems, vaccine preparations, and in any situation where it is required to obtain high amounts of heterologous proteins as fusion polypeptides in E. coli.

摘要翻译： PCT No.PCT / CU97 / 00001 Sec。 371日期：1997年9月16日 102（e）1997年9月16日PCT PCT 1997年1月17日PCT公布。 第WO97 / 26359号公报 日期1997年7月24日本发明涉及生物技术和遗传工程，特别是通过将重组DNA技术应用于细菌肽，通过其融合将微生物中的病毒来源的蛋白质的表达。 本发明提供了在大肠杆菌中将异源蛋白质作为融合多肽表达的有效方法，以便以商业上有用的量以高度纯度获得它们，并以适合的形式包含在旨在用于 人用。 为此，基本上使用的是来自脑膜炎奈瑟氏球菌B：4：P1.15的抗原P64k的前47个氨基酸的稳定化序列。 特别地，使用包含所述序列的重组质粒，其在大肠杆菌的色氨酸启动子和噬菌体T4的转录终止子的控制下，包括提供DNA片段相克隆的限制性位点 编码感兴趣的多肽。 本发明的方法适用于制药工业，用于开发诊断系统，疫苗制剂，并且在需要在大肠杆菌中获得大量异源蛋白质作为融合多肽的任何情况下。

公开(公告)号：US06921809B1

公开(公告)日：2005-07-26

申请号：US09612925

申请日：2000-07-10

申请人： Carlos Antonio Durate Cano ,  Enrique Gerardo Guillen Nieto ,  Anabel Alvarez Acosta ,  Luis Emilio Carpio Munoz ,  Diogenes Quintana Vazquez ,  Carmen Elena Gomez Rodriquez ,  Recardo De La Caridid Siva Rodriquez ,  Consuelo Nazabal Galvez ,  Maria De Jesus Leal Angulo ,  Alejandro Miguel Martin Dunn

发明人： Carlos Antonio Durate Cano ,  Enrique Gerardo Guillen Nieto ,  Anabel Alvarez Acosta ,  Luis Emilio Carpio Munoz ,  Diogenes Quintana Vazquez ,  Carmen Elena Gomez Rodriquez ,  Recardo De La Caridid Siva Rodriquez ,  Consuelo Nazabal Galvez ,  Maria De Jesus Leal Angulo ,  Alejandro Miguel Martin Dunn

IPC分类号： G01N33/569 ,  A61K39/00 ,  A61K39/095 ,  A61K39/395 ,  A61K39/40 ,  A61P31/04 ,  A61P31/12 ,  C07K1/22 ,  C07K14/16 ,  C07K14/22 ,  C07K16/00 ,  C07K16/12 ,  C07K19/00 ,  C12N1/21 ,  C12N15/02 ,  C12N15/31 ,  C12N15/48 ,  C12N15/62 ,  C12N15/70 ,  C12P21/02 ,  C12P21/08 ,  C12R1/19

CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K14/22 ,  C07K16/1217 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/75 ,  C12N15/62 ,  C12N2740/16122 ,  Y10S530/82 ,  Y10S530/825

摘要： The present invention related to biotechnology and genetic engineering, particularly the expression of proteins of viral origin in microorganisms through their fusion by applying recombinant DNA technology to bacterial peptides. The present invention provides an effcient process for the expression in Escherichia coli of heterlogous proteins as fusion peptides with a view to obtaining them with a high degree of purity, in commercially useful amounts, and in an appropriate form for their inclusion in vaccine preparations. What is essentially used is a stabilizing sequence derived from the first 47 amino acids of the antigen P64k of Neisseria meningitides B:4:P1.15. In particular, use is made of a recombinant plasmid containing said sequence, under the control of the tryptophane promoter of E. coli and of the terminator of the transcription of the phage T4, including restrictions sites which provide for the cloning in phase of DNA fragments coding for polypeptides of interest.

摘要翻译： 本发明涉及生物技术和遗传工程，特别是通过将重组DNA技术应用于细菌肽，通过其融合在微生物中表达病毒来源的蛋白质。 本发明提供了一种在大肠杆菌中将异源蛋白质作为融合肽表达的有效方法，以便以商业上有用的量获得高纯度的纯度，并以适合的形式包含在疫苗制剂中。 基本上使用的是来自脑膜炎奈瑟菌B：4：P1.15的抗原P64k的前47个氨基酸的稳定序列。 特别地，使用含有所述序列的重组质粒，其在大肠杆菌的色氨酸启动子和噬菌体T4的转录终止子的控制下，包括限制性位点，其提供DNA片段的同时性克隆 编码感兴趣的多肽。