公开(公告)号：US20050170460A1

公开(公告)日：2005-08-04

申请号：US11054192

申请日：2005-02-08

申请人： Volker Doring ,  Leslie Nangle ,  Tamara Hendrickson ,  Valerie Crecy-Lagard ,  Paul Schimmel ,  Philippe Marliere

发明人： Volker Doring ,  Leslie Nangle ,  Tamara Hendrickson ,  Valerie Crecy-Lagard ,  Paul Schimmel ,  Philippe Marliere

IPC分类号： C12N1/21 ,  C12N9/00 ,  C12N15/10 ,  C12N15/67 ,  C12P21/02 ,  C12P21/06 ,  C12N15/74

CPC分类号： C12N15/10 ,  C12N9/93 ,  C12N15/67 ,  C12P21/02 ,  C12R1/19

摘要： The present invention is directed to a method to diversify the chemical composition of proteins produced in vivo comprising the step of disabling, particularly by mutagenesis, the editing function of one of its aminoacyl tRNA synthetases. The present invention is also directed to nucleic acid sequences encoding such mutated aminoacyl tRNA synthetases having their editing site mutated and capable of mischarging its cognate tRNA with a noncanonical amino acid. Also described herein is an improved method for obtaining transformed cells capable of synthetizing in vivo proteins comprising at least a noncanonical amino acid and their use for the production of such proteins.

摘要翻译： 本发明涉及使体内产生的蛋白质的化学组成多样化的方法，其包括特别是通过诱变使其氨酰tRNA合成酶之一的编辑功能失活的步骤。 本发明还涉及编码这种突变的氨基酰基tRNA合成酶的核酸序列，其具有突变的编辑位点并且能够与非科学氨基酸对其同源tRNA进行错配。 本文还描述了一种用于获得能够合成体内蛋白质的转化细胞的改进方法，其包含至少一种非科学氨基酸及其用于产生这种蛋白质的用途。

公开(公告)号：US07524646B2

公开(公告)日：2009-04-28

申请号：US11054192

申请日：2005-02-08

申请人： Volker Doring ,  Leslie A. Nangle ,  Tamara L. Hendrickson ,  Valerie De Crecy-Lagard ,  Paul Schimmel ,  Philippe Marliere

发明人： Volker Doring ,  Leslie A. Nangle ,  Tamara L. Hendrickson ,  Valerie De Crecy-Lagard ,  Paul Schimmel ,  Philippe Marliere

IPC分类号： C12P21/06 ,  C12N9/10 ,  C12N9/12

CPC分类号： C12N15/10 ,  C12N9/93 ,  C12N15/67 ,  C12P21/02 ,  C12R1/19

摘要： The present invention is directed to a method to diversify the chemical composition of proteins produced in vivo comprising the step of disabling, particularly by mutagenesis, the editing function of one of its aminoacyl tRNA synthetases. The present invention is also directed to nucleic acid sequences encoding such mutated aminoacyl tRNA synthetases having their editing site mutated and capable of mischarging its cognate tRNA with a noncanonical amino acid. Also described herein is an improved method for obtaining transformed cells capable of synthetizing in vivo proteins comprising at least a noncanonical amino acid and their use for the production of such proteins.

摘要翻译： 本发明涉及使体内产生的蛋白质的化学组成多样化的方法，其包括特别是通过诱变使其氨酰tRNA合成酶之一的编辑功能失活的步骤。 本发明还涉及编码这种突变的氨基酰基tRNA合成酶的核酸序列，其具有突变的编辑位点并且能够与非科学氨基酸对其同源tRNA进行错配。 本文还描述了一种用于获得能够合成体内蛋白质的转化细胞的改进方法，其包含至少一种非科学氨基酸及其用于产生这种蛋白质的用途。

公开(公告)号：US20050038255A1

公开(公告)日：2005-02-17

申请号：US10479719

申请日：2002-06-10

申请人： Denis Thibaut ,  Volker Doring ,  Philippe Marliere

发明人： Denis Thibaut ,  Volker Doring ,  Philippe Marliere

IPC分类号： C12N15/09 ,  C07B53/00 ,  C07D211/60 ,  C07D241/04 ,  C07D279/12 ,  C12N1/21 ,  C12N9/88 ,  C12N15/60 ,  C12P13/04 ,  C12P17/10 ,  C12P17/12 ,  C12P41/00 ,  C07D211/30

CPC分类号： C12N9/88 ,  C12P13/04 ,  C12P17/10 ,  C12P17/12 ,  C12P41/006

摘要： The invention concerns a method for producing a cyclic L-amino acid of formula (I), characterised in that it consists in reacting a L-diamino acid of formula (II) or an enantiomeric mixture comprising such a L-diamino acid and a corresponding D-diamino acid in variable proportions, in the presence of an ornithine cyclodeaminase or a polypeptide homologous to the ornithine cyclodeaminase.

摘要翻译： 本发明涉及一种制备式（I）的环状L-氨基酸的方法，其特征在于其包括使式（II）的L-二氨基酸或包含这样的L-二氨基酸的对映异构体混合物与相应的 在鸟氨酸环脱氨酶或与鸟氨酸环脱氨酶同源的多肽的存在下，可变比例的D-二氨基酸。

公开(公告)号：US07736899B1

公开(公告)日：2010-06-15

申请号：US09830669

申请日：1999-10-28

申请人： Philippe Marliere ,  Volker Doring ,  Henning Mootz

发明人： Philippe Marliere ,  Volker Doring ,  Henning Mootz

IPC分类号： C12N15/00 ,  C12N1/20 ,  C12P21/00

CPC分类号： C12N15/1058 ,  A61K38/00 ,  A61K48/00 ,  C12N15/67 ,  C12P21/02

摘要： The present invention relates to a method for providing bacterial or yeast cells with the capacity to produce a protein, the amino acid sequence of which comprises at least one unconventional amino acid. The method involves (a) introducing at least one missense mutation in a target codon of a gene encoding a protein required for the growth of the bacterial or yeast cells, where the mutated protein synthesized from the mutated gene is not functional in the bacterial or yeast cells. The method also involves (b) selecting the bacterial or yeast cells obtained in (a) in a culture medium which (1) does not contain a nutrient compensating for the loss of functionality of the mutated protein and (2) contains an unconventional amino acid which restores the functionality of the protein required for growth of the bacterial or yeast cells, in which the unconventional amino acid is that encoded by the target codon. The method also involves culturing the bacterial or yeast cells obtained in (b) in a culture medium containing the amino acid encoded by the target codon.

摘要翻译： 本发明涉及提供具有产生蛋白质的能力的细菌或酵母细胞的方法，所述蛋白质的氨基酸序列包含至少一种非常规氨基酸。 该方法包括（a）在编码细菌或酵母细胞生长所需的蛋白质的基因的靶标密码子中引入至少一个错义突变，其中从突变基因合成的突变蛋白在细菌或酵母中不起作用 细胞。 该方法还包括（b）在培养基中选择（a）中获得的细菌或酵母细胞，其中（1）不含营养物，补偿突变蛋白的功能丧失，（2）含有非常规氨基酸 其恢复细菌或酵母细胞生长所需的蛋白质的功能，其中非常规氨基酸是由靶标密码子编码的。 该方法还包括在含有由目标密码子编码的氨基酸的培养基中培养（b）中获得的细菌或酵母细胞。

公开(公告)号：US20070212759A1

公开(公告)日：2007-09-13

申请号：US10560760

申请日：2004-06-24

申请人： Volker Doring ,  Denis Thibaut ,  Annett Kreimeyer ,  Philippe Marliere

发明人： Volker Doring ,  Denis Thibaut ,  Annett Kreimeyer ,  Philippe Marliere

IPC分类号： C12P19/30 ,  C07H5/04 ,  C07C45/29

CPC分类号： C07H1/00 ,  C07H7/027 ,  C12N9/88 ,  C12P7/24 ,  C12P7/42

摘要： This invention relates to a process for preparing 2′-deoxynucleoside compounds or 2′-deoxynucleoside precursors using 2-dehydro-3-deoxy-D-gluconic acid (usually abbreviated as KDG) or its salts as a starting material. A variety of 2′-deoxynucleosides and their analogues are used as a starting material for synthesis or drug formulation in production of an antiviral, anticancer or antisense agent.

摘要翻译： 本发明涉及使用2-脱氢-3-脱氧-D-葡萄糖酸（通常简写为KDG）或其盐作为起始原料制备2'-脱氧核苷化合物或2'-脱氧核苷前体的方法。 各种2'-脱氧核苷及其类似物在制备抗病毒，抗癌或反义剂时用作合成或药物制剂的起始原料。

公开(公告)号：US5704331A

公开(公告)日：1998-01-06

申请号：US778782

申请日：1997-01-06

申请人： Hermann Eberbach ,  Eberhard Mack ,  Volker Doring

发明人： Hermann Eberbach ,  Eberhard Mack ,  Volker Doring

IPC分类号： F02B67/04 ,  F16D3/02 ,  F02B77/00

CPC分类号： F02B67/04 ,  F16D3/02

摘要： In an accessory unit drive arrangement of an internal combustion engine the accessory unit is mounted on the engine such that its drive shaft is arranged in axial alignment with an engine-operated shaft. The drive shaft is coupled to the engine-operated shaft by a coupling structure which includes a pin which projects axially eccentrically from the end face of the engine-operated shaft and engages a carrier mounted on the drive shaft of the accessory unit. The engine-operated shaft is supported by a friction bearing and a lubricant collecting space is in communication with the friction bearing so as to collect lubricant leaking from the bearing for the lubrication of the coupling structure.

摘要翻译： 在内燃机的附件单元驱动装置中，附件单元安装在发动机上，使得其驱动轴与发动机操作的轴轴向对准地布置。 驱动轴通过联接结构联接到发动机操作的轴，该联接结构包括从发动机操纵的轴的端面轴向偏心地突出的销，并接合安装在附件单元的驱动轴上的载体。 发动机操纵的轴由摩擦轴承支撑，并且润滑剂收集空间与摩擦轴承连通，以便收集从轴承泄漏的润滑剂用于联接结构的润滑。

公开(公告)号：US20050169747A1

公开(公告)日：2005-08-04

申请号：US11032311

申请日：2005-01-10

申请人： Volker Doring ,  Hans-Dieter Hensel ,  Peter Schick

发明人： Volker Doring ,  Hans-Dieter Hensel ,  Peter Schick

IPC分类号： F01D17/16 ,  F01D17/20 ,  F03D7/00

CPC分类号： F01D17/167 ,  F01D17/20 ,  F02B37/025 ,  F02B39/00 ,  F05D2220/40 ,  Y02T10/144

摘要： In an exhaust gas turbine for a turbocharger of an internal combustion engine, including a housing with an inlet passage for conducting exhaust gas onto a turbine wheel supported in the housing, a guide vane structure movable into, and out of, an annular space of the inlet passage around the turbine wheel, an operating mechanism for moving the guide vane structure is provided which includes a cylinder, a ring provided with axial passages disposed around an inner sleeve, the guide vane structure being connected to one end of claws movably disposed in the axial passages and at least one of the claws being connected at its other end to the operating mechanism.

摘要翻译： 在用于内燃机的涡轮增压器的废气涡轮机中，包括具有用于将废气引导到支撑在所述壳体中的涡轮机叶轮上的入口通道的壳体，可移动到所述壳体的环形空间中并且离开所述壳体的环形空间的导叶结构 设置有用于移动导向叶片结构的操作机构，该操作机构包括一个气缸，设置有围绕内套管设置的轴向通道的环，所述导向叶片结构连接到可移动地设置在所述导向叶片结构中的爪的一端 轴向通道，并且至少一个爪的另一端连接到操作机构。

公开(公告)号：US4778997A

公开(公告)日：1988-10-18

申请号：US772420

申请日：1985-09-04

申请人： Volker Doring

发明人： Volker Doring

IPC分类号： G01T1/164 ,  G21K1/04

CPC分类号： G01T1/1648 ,  A61B6/4258

摘要： Process for depicting the distribution of high activities of radioactive substances by means of a whole-body scintigraphic device having a camera or the like, such as a gamma camera or scanner, characterized in that the radiation incident upon the camera is partially shielded against as a function of its intensity in order to optimize the counting rate, as well as a device for implementing such a process.

摘要翻译： 通过具有照相机等的全身闪烁照相装置（例如γ照相机或扫描仪）描绘放射性物质的高活性分布的方法，其特征在于，入射到照相机上的辐射被部分屏蔽 其强度的功能是为了优化计数率，以及用于实现这种过程的装置。

公开(公告)号：US20050008479A1

公开(公告)日：2005-01-13

申请号：US10616523

申请日：2003-07-10

申请人： Volker Doring ,  Hans-Dieter Hensel ,  Peter Schlick

发明人： Volker Doring ,  Hans-Dieter Hensel ,  Peter Schlick

IPC分类号： F01D17/14 ,  F03B1/04

CPC分类号： F01D17/143 ,  F05D2220/40 ,  F05D2270/62

摘要： In an exhaust gas turbine for a turbocharger of an internal combustion engine with a casing and a rotor rotatably supported in the casing, the casing includes an inlet duct with a guide vane structure supported so as to be movable into and out of a flow inlet area to the rotor by means of an electric motor.

摘要翻译： 在具有可旋转地支撑在壳体中的具有壳体和转子的内燃机的涡轮增压器的废气涡轮机中，壳体包括具有导向叶片结构的入口管道，该导向管道结构被支撑成可移动进入和流出流入口区域 通过电动机到达转子。

公开(公告)号：US06374611B2

公开(公告)日：2002-04-23

申请号：US09877711

申请日：2001-06-08

申请人： Volker Doring ,  Hans-Dieter Hensel ,  Peter Schick

发明人： Volker Doring ,  Hans-Dieter Hensel ,  Peter Schick

IPC分类号： F02B3344

CPC分类号： F02C6/12 ,  F05D2220/40 ,  F05D2250/15 ,  F05D2250/34 ,  F05D2250/35

摘要： In an exhaust turbine of a turbocharger with a rotor mounted rotatably in a turbine casing having a spiral inlet duct with a guide vane structure including guide vanes arranged around the rotor and extending essentially tangentially to an outer and inner imaginary circular surface areas in the inflow zone and, respectively, the outflow zone of the guide vane structure, at least the trailing edges but preferably also the leading edges of the guide vanes extend at an angle to an axial line of the imaginary circular surface areas, thereby providing for a uniform loading of the rotor.