公开(公告)号：US06316193B1

公开(公告)日：2001-11-13

申请号：US09412565

申请日：1999-10-05

申请人： Wei-Wu He ,  Gilbert Jay

发明人： Wei-Wu He ,  Gilbert Jay

IPC分类号： C12Q168

CPC分类号： C12N15/1034

摘要： The present invention relates to a arrays of nucleic acid and methods of screening these arrays for desired nucleotide sequences. In a preferred embodiment of the invention, a desired cDNA clone can be obtained in three or less rounds of PCR screening. A master plate containing a population of cDNA, distributed in a plurality of wells, is screened for a desired clone by PCR. After a master well containing the desired cDNA is identified, a second plate containing a cDNA array of the master well can then be screened using the same PCR primers. Since the second plate contains about 50-fold to 100-fold fewer clones than the master plate, an expedient reduction in the number of candidates can be achieved in a single PCR step. The invention also relates to a super-master plate containing at least two, preferably more, different populations of cDNA obtainable from different sources of mRNA.

摘要翻译： 本发明涉及核酸阵列和筛选所需核苷酸序列的这些阵列的方法。 在本发明的优选实施方案中，可以在三次或更少轮的PCR筛选中获得所需的cDNA克隆。 通过PCR筛选含有分布在多个孔中的cDNA群体的母板以获得期望的克隆。 鉴定含有所需cDNA的主孔后，可以使用相同的PCR引物筛选含有主孔cDNA序列的第二平板。 由于第二板含有比母板少约50倍至100倍的克隆，因此可以在单个PCR步骤中实现候选数目的有利的减少。 本发明还涉及含有可从不同mRNA来源获得的至少两个，优选更多不同种群的cDNA的超级主板。

公开(公告)号：US07083930B2

公开(公告)日：2006-08-01

申请号：US10650721

申请日：2003-08-29

申请人： Peter L. Hudson ,  Wei-Wu He ,  Craig A. Rosen ,  Jeannine D. Gocayne

发明人： Peter L. Hudson ,  Wei-Wu He ,  Craig A. Rosen ,  Jeannine D. Gocayne

IPC分类号： C12Q1/68 ,  C12Q1/48

CPC分类号： C12N9/1029 ,  A61K38/00

摘要： Human choline acetyltransferase polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptide for the treatment of cognitive and neurological deficiencies or mental disturbances such as degenerative nervous system disorders, for example, Alzheimer's Disease, ALS and other cholinergic defects, and antagonists for treating Parkinson's Disease and other disorders relating to an over-expression of acetylcholine. Also disclosed are diagnostic methods for detecting a mutation in the human Choline Acetyltransferase nucleic acid sequence.

公开(公告)号：US20050089984A1

公开(公告)日：2005-04-28

申请号：US10961148

申请日：2004-10-12

申请人： Vishva Dixit ,  Wei-Wu He ,  Kristine Kikly ,  Steven Ruben

发明人： Vishva Dixit ,  Wei-Wu He ,  Kristine Kikly ,  Steven Ruben

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/55 ,  A61K39/395 ,  A61K45/00 ,  A61K48/00 ,  A61P9/10 ,  A61P25/00 ,  A61P25/28 ,  A61P31/04 ,  A61P43/00 ,  C07K14/47 ,  C07K16/40 ,  C12N5/00 ,  C12N5/10 ,  C12N9/64 ,  C12N9/99 ,  C12N15/12 ,  C12Q1/68 ,  C12R1/91 ,  C07H21/04

CPC分类号： C12N9/6475 ,  A61K38/00 ,  A61K48/00

摘要： Human ICE LAP-6 polypeptides and DNA (RNA) encoding such ICE LAP-6 and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such ICE LAP-6 for the treatment of a susceptibility to viral infection, tumorogenesis and to diseases and defects in the control embryogenesis and tissue homeostasis, and the nucleic acid sequences described above may be employed in an assay for ascertaining such susceptibility. Antagonists against such ICE LAP-6 and their use as a therapeutic to treat Alzheimer's disease, Parkinson's disease, rheumatoid arthritis, septic shock, sepsis, stroke, chronic inflammation, acute inflammation, CNS inflammation, osteoporosis, ischemia reperfusion injury, cell death associated with cardiovascular disease, polycystic kidney disease, apoptosis of endothelial cells in cardiovascular disease, degenerative liver disease, MS, ALS, cererbellar degeneration, ischemic injury, myocardial infarction, AIDS, myelodysplastic syndromes, aplastic anemia, male pattern baldness, and head injury damage are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to mutations in the nucleic acid sequences and altered concentrations of the polypeptides. Also disclosed are diagnostic assays for detecting mutations in the polynucleotides encoding the interleukin-1 beta converting enzyme apoptosis proteases and for detecting altered levels of the polypeptide in a host.

摘要翻译： 公开了人ICE LAP-6多肽和编码这种ICE LAP-6的DNA（RNA）和通过重组技术产生这种多肽的方法。 还公开了利用这种ICE LAP-6用于治疗对病毒感染，肿瘤发生的易感性以及控制胚胎发生和组织稳态中的疾病和缺陷的方法，并且上述核酸序列可用于确定 这种敏感性。 对抗这种ICE LAP-6的拮抗剂及其作为治疗性阿尔茨海默病，帕金森病，类风湿性关节炎，败血性休克，败血症，中风，慢性炎症，急性炎症，CNS炎症，骨质疏松症，缺血再灌注损伤，与 心血管疾病，多囊肾病，心血管疾病中的内皮细胞凋亡，退行性肝病，MS，ALS，小脑变性，缺血性损伤，心肌梗塞，艾滋病，骨髓增生异常综合征，再生障碍性贫血，男性型秃发和头部损伤也是 披露 还公开了用于检测与核酸序列中的突变和多肽的改变的浓度相关的疾病的诊断测定。 还公开了用于检测编码白细胞介素-1β转换酶凋亡蛋白酶的多核苷酸中的突变并用于检测宿主中多肽的改变水平的诊断测定法。

公开(公告)号：US06432708B1

公开(公告)日：2002-08-13

申请号：US09939573

申请日：2001-08-28

申请人： Peter L. Hudson ,  Wei-Wu He ,  Craig A. Rosen ,  Jeannine D. Gocayne

发明人： Peter L. Hudson ,  Wei-Wu He ,  Craig A. Rosen ,  Jeannine D. Gocayne

IPC分类号： C12N516

CPC分类号： C12N9/1029 ,  A61K38/00

摘要： Human choline acetyltransferase polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptide for the treatment of cognitive and neurological deficiencies or mental disturbances such as degenerative nervous system disorders, for example, Alzheimer's Disease, ALS and other cholinergic defects, and antagonists for treating Parkinson's Disease and other disorders relating to an over-expression of acetylcholine. Also disclosed are diagnostic methods for detecting a mutation in the human Choline Acetyltransferase nucleic acid sequence.

摘要翻译： 公开了人胆碱乙酰转移酶多肽和编码这种多肽的DNA（RNA）和通过重组技术产生这种多肽的方法。 还公开了利用这种多肽治疗认知和神经缺陷或精神障碍如退行性神经系统疾病例如阿尔茨海默氏病，ALS和其它胆碱能缺陷的治疗方法，以及用于治疗帕金森氏病和其他与 过度表达乙酰胆碱。 还公开了用于检测人胆碱乙酰转移酶核酸序列中的突变的诊断方法。

公开(公告)号：US20120231969A1

公开(公告)日：2012-09-13

申请号：US13497895

申请日：2010-09-23

申请人： Wei-Wu He ,  Donghui Ma ,  Youmin Shu ,  Zairen Sun

发明人： Wei-Wu He ,  Donghui Ma ,  Youmin Shu ,  Zairen Sun

IPC分类号： C40B30/04 ,  C40B40/10 ,  C40B50/06

CPC分类号： G01N33/6845

摘要： Illustrative embodiments herein disclosed relate to protein arrays, methods for making the arrays and methods for using them, among others. In some embodiments known proteins representing at least 50% of the loci in the human genome are arrayed in known positions on a support. In some embodiments arrays are made of proteins purified from cell lysates by affinity binding to the support. In some embodiments protein arrays are used to decode the binding specificity of antibodies. In some embodiments protein arrays are used to diagnose auto-immune disorders. Many other embodiments and general features are disclosed.

摘要翻译： 本文公开的说明性实施方案涉及蛋白质阵列，制备阵列的方法及其使用方法等。 在一些实施方案中，代表人类基因组中至少50％的位点的已知蛋白质排列在载体上的已知位置。 在一些实施方案中，阵列由通过亲和结合载体从细胞裂解物纯化的蛋白质制成。 在一些实施方案中，蛋白质阵列用于解码抗体的结合特异性。 在一些实施方案中，蛋白质阵列用于诊断自身免疫疾病。 公开了许多其它实施例和一般特征。

公开(公告)号：US20120115923A1

公开(公告)日：2012-05-10

申请号：US12087413

申请日：2006-12-29

申请人： Wei-Wu He ,  Patrick Y. Lu

发明人： Wei-Wu He ,  Patrick Y. Lu

IPC分类号： A61K31/713 ,  C12N15/113 ,  C12N5/071 ,  A61P29/00 ,  A61P17/02 ,  C12N15/11 ,  A61K31/7105

CPC分类号： C12N15/1136 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/31

摘要： This invention describes compositions and methods using siRNA to target various genes expressed in cells of injured tissue during scar formation to promote scar-free wound healing.

摘要翻译： 本发明描述了使用siRNA靶向在瘢痕形成期间损伤组织细胞中表达的各种基因以促进无疤痕伤口愈合的组合物和方法。

公开(公告)号：US20080227127A1

公开(公告)日：2008-09-18

申请号：US12059797

申请日：2008-03-31

申请人： Wei-Wu He ,  Kenneth C. Carter

发明人： Wei-Wu He ,  Kenneth C. Carter

IPC分类号： G01N33/53 ,  C07H21/00 ,  C12N15/63 ,  C12N15/00 ,  C12N1/21 ,  C07K7/08 ,  C07K7/06 ,  C12N7/00 ,  C12N5/10 ,  C07K14/00 ,  C07K16/00

CPC分类号： A61K31/555 ,  A61K38/00 ,  A61K48/00 ,  A61N5/062 ,  C07K14/4702 ,  C07K2319/00 ,  C12N2799/026

摘要： The present invention relates to a novel member of the NK family of homeobox genes. In particular, isolated nucleic acid molecules are provided encoding the human NK-3 prostate specific gene 1 (NKX3.1) protein. NKX3.1 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of NKX3.1 activity. Also provided are diagnostic methods for detecting prostate cancer and other cancers and therapeutic methods for prostate cancer and other cancers.

摘要翻译： 本发明涉及同源盒基因的NK家族的新成员。 特别地，提供编码人NK-3前列腺特异性基因1（NKX3.1）蛋白的分离的核酸分子。 还提供NKX3.1多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明还涉及用于鉴定NKX3.1活性的激动剂和拮抗剂的筛选方法。 还提供了用于检测前列腺癌和其他癌症的诊断方法以及用于前列腺癌和其它癌症的治疗方法。

公开(公告)号：US07115260B2

公开(公告)日：2006-10-03

申请号：US10961148

申请日：2004-10-12

申请人： Vishva M. Dixit ,  Wei-Wu He ,  Kristine K. Kikly ,  Steven M. Ruben

发明人： Vishva M. Dixit ,  Wei-Wu He ,  Kristine K. Kikly ,  Steven M. Ruben

IPC分类号： A61K38/17 ,  A61K38/18 ,  A61K39/00 ,  C12P21/00

CPC分类号： C12N9/6475 ,  A61K38/00 ,  A61K48/00

摘要： Human ICE LAP-6 polypeptides and DNA (RNA) encoding such ICE LAP-6 and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such ICE LAP-6 for the treatment of a susceptibility to viral infection, tumorogenesis and to diseases and defects in the control embryogenesis and tissue homeostasis, and the nucleic acid sequences described above may be employed in an assay for ascertaining such susceptibility. Antagonists against such ICE LAP-6 and their use as a therapeutic to treat Alzheimer's disease, Parkinson's disease, rheumatoid arthritis, septic shock, sepsis, stroke, chronic inflammation, acute inflammation, CNS inflammation, osteoporosis, ischemia reperfusion injury, cell death associated with cardiovascular disease, polycystic kidney disease, apoptosis of endothelial cells in cardiovascular disease, degenerative liver disease, MS, ALS, cererbellar degeneration, ischemic injury, myocardial infarction, AIDS, myelodysplastic syndromes, aplastic anemia, male pattern baldness, and head injury damage are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to mutations in the nucleic acid sequences and altered concentrations of the polypeptides. Also disclosed are diagnostic assays for detecting mutations in the polynucleotides encoding the interleukin-1 beta converting enzyme apoptosis proteases and for detecting altered levels of the polypeptide in a host.

摘要翻译： 公开了人ICE LAP-6多肽和编码这种ICE LAP-6的DNA（RNA）和通过重组技术产生这种多肽的方法。 还公开了利用这种ICE LAP-6用于治疗对病毒感染，肿瘤发生的易感性以及控制胚胎发生和组织稳态中的疾病和缺陷的方法，并且上述核酸序列可用于确定 这种敏感性。 对抗这种ICE LAP-6的拮抗剂及其作为治疗性阿尔茨海默病，帕金森病，类风湿性关节炎，败血性休克，败血症，中风，慢性炎症，急性炎症，CNS炎症，骨质疏松症，缺血再灌注损伤，与 心血管疾病，多囊肾病，心血管疾病中的内皮细胞凋亡，退行性肝病，MS，ALS，小脑变性，缺血性损伤，心肌梗塞，艾滋病，骨髓增生异常综合征，再生障碍性贫血，男性型秃发和头部损伤也是 披露 还公开了用于检测与核酸序列中的突变和多肽的改变的浓度相关的疾病的诊断测定。 还公开了用于检测编码白细胞介素-1β转换酶凋亡蛋白酶的多核苷酸中的突变并用于检测宿主中多肽的改变水平的诊断测定法。

公开(公告)号：US06835555B1

公开(公告)日：2004-12-28

申请号：US09613508

申请日：2000-07-10

申请人： Wei-Wu He ,  Craig A. Rosen ,  Peter L. Hudson ,  Gregg A. Hastings

发明人： Wei-Wu He ,  Craig A. Rosen ,  Peter L. Hudson ,  Gregg A. Hastings

IPC分类号： C12P2102

CPC分类号： C12N9/6475 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/00

摘要： Disclosed are human interleukin-1 &bgr; converting enzyme like apoptosis proteases-3 and 4 and DNA (RNA) encoding such polypeptides. Also provided is a procedure for producing such polypeptides by recombinant techniques and antibodies and antagonists against such polypeptides. Also provided are methods of using the polypeptides, for example, as an antitumor agent, and antiviral agent, and antibodies and antagonists against such polypeptides for example, for treating Alzheimer's disease, Parkinson's disease, rheumatoid arthritis and head injury. Diagnostic assays for identifying mutations in nucleic acid sequence encoding a polypeptide of the present invention and for detecting altered levels of the polypeptide of the present invention for detecting diseases are also disclosed.

摘要翻译： 公开了人白细胞介素-1β转换酶如凋亡蛋白酶-3和4以及编码这种多肽的DNA（RNA）。 还提供了通过重组技术产生这种多肽的方法以及针对这种多肽的抗体和拮抗剂。 还提供了使用多肽例如抗肿瘤剂和抗病毒剂的方法，以及抗这种多肽的抗体和拮抗剂，例如用于治疗阿尔茨海默病，帕金森病，类风湿性关节炎和头部损伤。 还公开了用于鉴定编码本发明多肽的核酸序列中的突变并用于检测用于检测疾病的本发明多肽的改变水平的诊断测定。

公开(公告)号：US07892773B2

公开(公告)日：2011-02-22

申请号：US12059797

申请日：2008-03-31

申请人： Wei-Wu He ,  Kenneth C. Carter

发明人： Wei-Wu He ,  Kenneth C. Carter

IPC分类号： G01N33/53 ,  C07K16/00 ,  C07K7/08 ,  C12N7/00 ,  C12N5/10 ,  C12N15/63 ,  C07K14/00

CPC分类号： A61K31/555 ,  A61K38/00 ,  A61K48/00 ,  A61N5/062 ,  C07K14/4702 ,  C07K2319/00 ,  C12N2799/026

摘要： The present invention relates to a novel member of the NK family of homeobox genes. In particular, isolated nucleic acid molecules are provided encoding the human NK-3 prostate specific gene 1 (NKX3.1) protein. NKX3.1 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of NKX3.1 activity. Also provided are diagnostic methods for detecting prostate cancer and other cancers and therapeutic methods for prostate cancer and other cancers.

摘要翻译： 本发明涉及同源盒基因的NK家族的新成员。 特别地，提供编码人NK-3前列腺特异性基因1（NKX3.1）蛋白的分离的核酸分子。 还提供NKX3.1多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明还涉及用于鉴定NKX3.1活性的激动剂和拮抗剂的筛选方法。 还提供了用于检测前列腺癌和其他癌症的诊断方法以及用于前列腺癌和其它癌症的治疗方法。