公开(公告)号：US07569375B2

公开(公告)日：2009-08-04

申请号：US10666205

申请日：2003-09-18

申请人： Wolfgang Stampfer ,  Birgit Kosjek ,  Wolfgang Kroutil ,  Kurt Faber ,  Jürgen Eck ,  Frank Niehaus

发明人： Wolfgang Stampfer ,  Birgit Kosjek ,  Wolfgang Kroutil ,  Kurt Faber ,  Jürgen Eck ,  Frank Niehaus

IPC分类号： C12N9/04 ,  C12N15/00 ,  C12P21/04 ,  C12N1/20 ,  C12Q1/00 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12P7/04 ,  C12P41/002

摘要： The invention relates to biocatalysts showing alcohol dehydrogenase activity obtainable from Rhodococcus ruber, their preparation, their use in the oxidation of secondary alcohols and/or the reduction of ketones, as well as nucleic acids coding for these alcohol dehydrogenases and microorganisms transformed with nucleic acids coding for these biocatalysts and their use for producing the biocatalyst or oxidizing secondary alcohols and/or reducing ketones.

摘要翻译： 本发明涉及显示可从红球菌获得的醇脱氢酶活性的生物催化剂，它们的制备，它们在仲醇氧化中的用途和/或还原酮，以及编码这些醇脱氢酶的核酸和用编码的核酸转化的微生物 这些生物催化剂及其用于生产生物催化剂或氧化仲醇和/或还原酮的用途。

公开(公告)号：US08679829B2

公开(公告)日：2014-03-25

申请号：US10559583

申请日：2004-06-02

申请人： Christa Schleper ,  Melanie Jonuscheit ,  Jürgen Eck ,  Frank Niehaus ,  Sonja-Verena Albers ,  Sabrina Froels

发明人： Christa Schleper ,  Melanie Jonuscheit ,  Jürgen Eck ,  Frank Niehaus ,  Sonja-Verena Albers ,  Sabrina Froels

IPC分类号： C12N15/00 ,  C12N15/866 ,  C12N15/70 ,  C12N15/11

CPC分类号： C12N15/74

摘要： The present invention relates to a sulfolobus expression vector comprising: (a) sulfolobus origin of replication; (b) the genes encoding the structural proteins and the site-specific integrase of SSV1, SSV2 or pSSVx, operatively linked to expression control sequences and a packaging signal; (c) one or more selectable marker gene(s), operatively linked to sulfolobus expression control sequences; and (d) a sulfolobus promoter followed 3′ by a restriction enzyme recognition site or a multiple cloning site for insertion of a gene of interest and optionally a 3′ regulatory element. Moreover, the present invention relates to a shuttle vector comprising the sequences of the expression vector of the invention and additional sequences for propagation and selection in E. coli, wherein the additional sequences comprise (a) an E. coli on of replication; and (b) a marker for selection in E. coli. Furthermore, the invention relates to host cells transformed with the expression vector as well as to a kit comprising a vector or a host cell of the present invention. Finally, the present application also relates to a method for generating infectious subviral particles.

摘要翻译： 本发明涉及一种磺叶病毒表达载体，其包含：（a）磺基梭菌复制起点; （b）编码与表达控制序列和包装信号有效连接的结构蛋白和SSV1，SSV2或pSSVx的位点特异性整合酶的基因; （c）一个或多个可选择的标记基因，其可操作地连接到子宫颈表达控制序列; 和（d）通过限制性酶识别位点或用于插入目的基因和任选的3'调节元件的多克隆位点的3'端的磺基叶绿体启动子。 此外，本发明涉及包含本发明的表达载体的序列和在大肠杆菌中扩增和选择的其它序列的穿梭载体，其中所述另外的序列包含（a）复制的大肠杆菌; 和（b）用于在大肠杆菌中选择的标记物。 此外，本发明涉及用表达载体转化的宿主细胞以及包含本发明的载体或宿主细胞的试剂盒。 最后，本申请还涉及产生感染性亚病毒颗粒的方法。

公开(公告)号：US08623810B2

公开(公告)日：2014-01-07

申请号：US13254157

申请日：2010-03-03

申请人： Frank Niehaus ,  Jürgen Eck ,  Renate Schulze ,  Michael Krohn

发明人： Frank Niehaus ,  Jürgen Eck ,  Renate Schulze ,  Michael Krohn

IPC分类号： C12N9/48 ,  C12N9/50 ,  C12N15/52 ,  C12N15/57 ,  C12P21/00 ,  A61K38/43 ,  A61K38/48 ,  A61K48/00 ,  A61P17/02

CPC分类号： C12N9/6408

摘要： We have identified by molecular cloning a protease which originates from the larvae of Lucilia sericata and which was termed debrilase due to its activities useful for debridement of wounds. Described is a nucleic acid molecule encoding a serine protease having the ability to cleave fibrin and casein which is (a) a nucleic acid molecule encoding the serine protease comprising or consisting of the amino acid sequence of SEQ ID NO: 4 as well as to nucleic acid molecules encoding precursors or fragments of said serine protease; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 3; (c) a nucleic acid molecule encoding a serine protease the amino acid sequence of which is at least 80% identical to the amino acid sequence of (a), preferably at least 85% identical, more preferably at least 90% identical, and most preferred 95% identical; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 80% identical to the nucleotide sequence of (b), preferably at least 85% identical, more preferably at least 90% identical, and most preferred 95% identical; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (b) or (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.

摘要翻译： 我们已经通过分子克隆鉴定了源自丝蚕幼虫的蛋白酶，由于其可用于清创伤口的活性，所以被称为脱氨酶。 描述了编码具有裂解纤维蛋白和酪蛋白的能力的丝氨酸蛋白酶的核酸分子，其是（a）编码包含或由SEQ ID NO：4的氨基酸序列组成的丝氨酸蛋白酶的核酸分子以及核酸 编码所述丝氨酸蛋白酶的前体或片段的酸分子; （b）包含SEQ ID NO：3的核苷酸序列或由其组成的核酸分子; （c）编码丝氨酸蛋白酶的核酸分子，其氨基酸序列与（a）的氨基酸序列至少80％相同，优选至少85％相同，更优选至少90％相同，最多 优选95％相同; （d）核酸分子，其包含与（b）的核苷酸序列至少80％同一性，优选至少85％相同，更优选至少90％相同，最优选95％的核苷酸序列或由其组成， 相同; （e）相对于（b）或（d）的核酸分子简并的核酸分子; 或（f）对应于（a）至（d）中任一项的核酸分子的核酸分子，其中T被U.替代

公开(公告)号：US20120093788A1

公开(公告)日：2012-04-19

申请号：US13254157

申请日：2010-03-03

申请人： Frank Niehaus ,  Jürgen Eck ,  Renate Schulze ,  Michael Krohn

发明人： Frank Niehaus ,  Jürgen Eck ,  Renate Schulze ,  Michael Krohn

IPC分类号： A61K48/00 ,  C12N9/50 ,  A61K36/06 ,  A61K36/064 ,  A61P17/02 ,  C07H21/04 ,  A61P17/00 ,  C12N1/21 ,  C12N1/19 ,  C12N15/63 ,  A61K31/7088 ,  C07K16/40 ,  A61K38/48 ,  A61K35/74

CPC分类号： C12N9/6408

摘要： We have identified by molecular cloning a protease which originates from the larvae of Lucilia sericata and which was termed debrilase due to its activities useful for debridement of wounds. Described is a nucleic acid molecule encoding a serine protease having the ability to cleave fibrin and casein which is (a) a nucleic acid molecule encoding the serine protease comprising or consisting of the amino acid sequence of SEQ ID NO: 4 as well as to nucleic acid molecules encoding precursors or fragments of said serine protease; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 3; (c) a nucleic acid molecule encoding a serine protease the amino acid sequence of which is at least 80% identical to the amino acid sequence of (a), preferably at least 85% identical, more preferably at least 90% identical, and most preferred 95% identical; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 80% identical to the nucleotide sequence of (b), preferably at least 85% identical, more preferably at least 90% identical, and most preferred 95% identical; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (b) or (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.

摘要翻译： 我们已经通过分子克隆鉴定了源自丝蚕幼虫的蛋白酶，由于其可用于清创伤口的活性，所以被称为脱氨酶。 描述了编码具有裂解纤维蛋白和酪蛋白的能力的丝氨酸蛋白酶的核酸分子，其是（a）编码包含或由SEQ ID NO：4的氨基酸序列组成的丝氨酸蛋白酶的核酸分子以及核酸 编码所述丝氨酸蛋白酶的前体或片段的酸分子; （b）包含SEQ ID NO：3的核苷酸序列或由其组成的核酸分子; （c）编码丝氨酸蛋白酶的核酸分子，其氨基酸序列与（a）的氨基酸序列至少80％相同，优选至少85％相同，更优选至少90％相同，最多 优选95％相同; （d）核酸分子，其包含与（b）的核苷酸序列至少80％相同，优选至少85％相同，更优选至少90％相同，最优选95％的核苷酸序列或由其组成， 相同; （e）相对于（b）或（d）的核酸分子简并的核酸分子; 或（f）对应于（a）至（d）中任一项的核酸分子的核酸分子，其中T被U.替代

公开(公告)号：US20110268792A1

公开(公告)日：2011-11-03

申请号：US13130128

申请日：2009-11-13

申请人： Andreas Buthe ,  Andreas Hafner ,  Franz Kaufmann ,  Esther Gabor ,  Guido Meurer ,  Jürgen Eck ,  Gordon Bradley

发明人： Andreas Buthe ,  Andreas Hafner ,  Franz Kaufmann ,  Esther Gabor ,  Guido Meurer ,  Jürgen Eck ,  Gordon Bradley

IPC分类号： A61K9/48 ,  A61P39/00 ,  A61K9/16 ,  A61K9/02 ,  A61K9/00 ,  A61K9/38

CPC分类号： A61K9/4891 ,  A61K9/0065 ,  A61K9/2063 ,  A61K9/2095 ,  A61K9/2873 ,  C07K14/37

摘要： The invention relates to a use of surface active hydrophobins for applications in pharmaceutical technology, in particular as excipients for galenic use. Provided is a method for either admixture of hydrophobins to galenic compositions or for treating the surface of pharmaceutical forms with a hydrophobin-containing solution to modify the pharmaceutical properties of the galenic form. In a preferred embodiment of the invention hydrophobins are used to improve the properties of a pharmaceutical composition, e.g. to act as a surfactant or to increase resistance to disintegration of the galenic forms to achieve a retarded drug release. The galenic form to be modified by the use of surface active proteins as excipients can be capsules, tablets, pills, microparticles, vesicles, and suppositories, although further galenic forms are envisioned. The surface active proteins used for the purpose of present invention can either be isolated from their respective natural source or prepared by recombinant techniques and expression in a suitable host.

摘要翻译： 本发明涉及用于制药技术中的表面活性疏水蛋白的用途，特别是作为涂覆液使用的赋形剂。 提供了将疏水蛋白与盖仑组合物混合或用含疏水蛋白的溶液处理药物形式的表面以改变盖仑膜形式的药物性质的方法。 在本发明的优选实施方案中，疏水蛋白用于改善药物组合物的性质，例如， 作为表面活性剂或增加耐受霜冻形式的崩解以实现延缓药物释放。 通过使用表面活性蛋白质作为赋形剂来修饰的盖仑形式可以是胶囊，片剂，丸剂，微粒，囊泡和栓剂，尽管可以预见到更多的盖仑制剂形式。 用于本发明目的的表面活性蛋白质可以从它们各自的天然来源中分离或者通过重组技术制备并在合适的宿主中表达。

公开(公告)号：US06271368B1

公开(公告)日：2001-08-07

申请号：US08776059

申请日：1997-06-19

申请人： Hans Lentzen ,  Jürgen Eck ,  Axel Baur ,  Holger Zinke

发明人： Hans Lentzen ,  Jürgen Eck ,  Axel Baur ,  Holger Zinke

IPC分类号： C12N121

CPC分类号： C07K14/42 ,  A61K38/00 ,  A61K47/6819 ,  C07K2319/00

摘要： The invention relates to nucleic acid molecules encoding preproproteins having after maturation the biological activity of the mistletoe lectin dimer, to vectors comprising these nucleic acid molecules, to hosts transformed with said vectors and to polypeptides and/or polypeptide dimers which are encoded by these nucleic acid molecules. The polypeptides and/or polypeptide dimers of the invention are widely therapeutically applicable. Thus, the present invention further relates to immunotoxins as well as to pharmaceutical compositions that contain the polypeptides and/or the polypeptide dimers of the invention. Additionally, the invention relates to diagnostic compositions comprising the nucleic acid molecules of the invention, the polypeptides and/or the polypeptide dimers of the invention and/or primers which hybridize specifically to the nucleic acid molecules of the invention. Finally, the invention relates to plant protective agents comprising the polypeptides of the invention and/or the polypeptide dimers of the invention.

摘要翻译： 本发明涉及将成熟后槲皮素凝集素二聚体的生物活性的前蛋白质，包含这些核酸分子的载体转染到用所述载体转化的宿主的核酸分子和由这些核酸编码的多肽和/或多肽二聚体 分子。 本发明的多肽和/或多肽二聚体在广泛的治疗上是适用的。 因此，本发明还涉及免疫毒素以及含有本发明的多肽和/或多肽二聚体的药物组合物。 另外，本发明涉及包含本发明的核酸分子，本发明的多肽和/或多肽二聚体的诊断组合物和/或与本发明的核酸分子特异性杂交的引物。 最后，本发明涉及包含本发明的多肽和/或本发明的多肽二聚体的植物保护剂。

公开(公告)号：US08226967B2

公开(公告)日：2012-07-24

申请号：US13130128

申请日：2009-11-13

申请人： Andreas Buthe ,  Andreas Hafner ,  Franz Kaufmann ,  Esther Gabor ,  Guido Meurer ,  Jürgen Eck ,  Gordon Bradley

发明人： Andreas Buthe ,  Andreas Hafner ,  Franz Kaufmann ,  Esther Gabor ,  Guido Meurer ,  Jürgen Eck ,  Gordon Bradley

IPC分类号： A01N25/34 ,  C07K1/00

CPC分类号： A61K9/4891 ,  A61K9/0065 ,  A61K9/2063 ,  A61K9/2095 ,  A61K9/2873 ,  C07K14/37

摘要： The invention relates to a use of surface active hydrophobins for applications in pharmaceutical technology, in particular as excipients for galenic use. Provided is a method for either admixture of hydrophobins to galenic compositions or for treating the surface of pharmaceutical forms with a hydrophobin-containing solution to modify the pharmaceutical properties of the galenic form. In a preferred embodiment of the invention hydrophobins are used to improve the properties of a pharmaceutical composition, e.g. to act as a surfactant or to increase resistance to disintegration of the galenic forms to achieve a retarded drug release. The galenic form to be modified by the use of surface active proteins as excipients can be capsules, tablets, pills, microparticles, vesicles, and suppositories, although further galenic forms are envisioned. The surface active proteins used for the purpose of present invention can either be isolated from their respective natural source or prepared by recombinant techniques and expression in a suitable host.

摘要翻译： 本发明涉及用于制药技术中的表面活性疏水蛋白的用途，特别是作为涂覆液使用的赋形剂。 提供了将疏水蛋白与盖仑组合物混合或用含疏水蛋白的溶液处理药物形式的表面以改变盖仑膜形式的药物性质的方法。 在本发明的优选实施方案中，疏水蛋白用于改善药物组合物的性质，例如， 作为表面活性剂或增加耐受霜冻形式的崩解以实现延缓药物释放。 通过使用表面活性蛋白质作为赋形剂来修饰的盖仑形式可以是胶囊，片剂，丸剂，微粒，囊泡和栓剂，尽管可以预见到更多的盖仑制剂形式。 用于本发明目的的表面活性蛋白质可以从其各自的天然来源分离或通过重组技术制备并在合适的宿主中表达。

公开(公告)号：US20100047863A1

公开(公告)日：2010-02-25

申请号：US12296057

申请日：2007-04-05

申请人： Jürgen Eck ,  Klaus Liebeton ,  Lutz Fischer ,  Sabine Lutz-Wahl ,  Martina Hensel ,  Christian Ewert ,  Marcus Wälz

发明人： Jürgen Eck ,  Klaus Liebeton ,  Lutz Fischer ,  Sabine Lutz-Wahl ,  Martina Hensel ,  Christian Ewert ,  Marcus Wälz

IPC分类号： C12P21/06 ,  C12P13/02 ,  C12N15/74 ,  C12N1/21 ,  C12N15/63 ,  C07K14/00 ,  C07K16/00 ,  C07H21/04

CPC分类号： C12N9/88 ,  C12P13/02 ,  C12P41/006

摘要： The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E.C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitrites.

摘要翻译： 本发明提供了编码具有腈水合酶（NHase）[E.C.]的酶的多核苷酸或一对多核苷酸。 4.2.1.84]活动。 此外，提供了包含所公开的多核苷酸或一对多核苷酸的载体和宿主及其生产方法。 此外，本发明涉及一对多肽或具有NHase活性的融合蛋白，特异性结合所述一对多肽或融合蛋白的抗体，在严格条件下与所公开的多核苷酸特异性杂交的引物或探针，或者 一对多核苷酸，包含多核苷酸或一对多核苷酸的组合物，一对多肽或融合蛋白，本发明的抗体和/或一种或多种引物或探针以及生产酰胺的方法，其包括对映选择性转化 亚硝酸盐

公开(公告)号：US07635567B2

公开(公告)日：2009-12-22

申请号：US10499297

申请日：2002-12-20

申请人： Johannes Müthing ,  Jasna Peter-Katalinic ,  Martin Langer ,  Babette Möckel ,  Jürgen Eck

发明人： Johannes Müthing ,  Jasna Peter-Katalinic ,  Martin Langer ,  Babette Möckel ,  Jürgen Eck

IPC分类号： G01N33/53 ,  G01N33/567

CPC分类号： C12Q1/48 ,  C07K16/18 ,  G01N33/566 ,  G01N33/66 ,  G01N2333/42 ,  G01N2400/00 ,  G01N2800/52

摘要： A method for the determination of the responsiveness of an individual to mistletoe lectin or to (an) mistletoe lectin single chain(s), wherein the expression of a membrane-bound receptor for mistletoe lectin is characteristic of a corresponding responsiveness.

摘要翻译： 用于测定个体对槲寄生凝集素或（一种）槲寄生凝集素单链的响应性的方法，其中槲寄生凝集素的膜结合受体的表达是相应的响应性的特征。

公开(公告)号：US09353386B2

公开(公告)日：2016-05-31

申请号：US14125409

申请日：2012-06-15

申请人： Daniel Koch ,  Guido Meurer ,  Jürgen Eck

发明人： Daniel Koch ,  Guido Meurer ,  Jürgen Eck

IPC分类号： C12P7/24 ,  C12P7/18 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10

CPC分类号： C12P7/24 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/13 ,  C12P7/18 ,  C12Y101/01077 ,  C12Y102/01 ,  C12Y208/03001 ,  Y02P20/52

摘要： The present invention relates to a host cell having an elevated expression or activity of an enzyme as compared with the parent cell from which it has been derived, said enzyme having lactoyl-CoA reductase activity. Furthermore, provided is a method of producing lactaldehyde and/or 1,2-propanediol, said method comprising culturing said host cell and/or utilizing said enzyme to produce said compound.

摘要翻译： 本发明涉及一种具有升高的酶表达或活性的宿主细胞，与其衍生的亲本细胞相比，所述酶具有乳酰基-CoA还原酶活性。 此外，提供了生产乳醛和/或1,2-丙二醇的方法，所述方法包括培养所述宿主细胞和/或利用所述酶产生所述化合物。