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公开(公告)号：US09206404B2
公开(公告)日：2015-12-08
申请号：US12592852
申请日：2009-12-03
申请人： Xiaoxia Cui , Aron M. Geurts , Fyodor Urnov
发明人： Xiaoxia Cui , Aron M. Geurts , Fyodor Urnov
IPC分类号： C12N15/00 , C12N9/22 , A01K67/027 , C12N15/85
CPC分类号： C12N15/907 , A01K67/0275 , A01K67/0276 , A01K67/0278 , A01K2217/054 , A01K2217/15 , A01K2227/10 , A01K2227/105 , A01K2267/03 , A01K2267/0393 , C07K2319/00 , C07K2319/81 , C12N9/22 , C12N15/1024 , C12N15/8509 , C12N2015/8527 , C12N2800/80
摘要： Disclosed herein are methods and compositions for genome editing of one or more loci in a rat, using fusion proteins comprising a zinc-finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
摘要翻译： 本文公开了使用包含锌指蛋白和切割结构域或裂解半结构域的融合蛋白进行大鼠中一个或多个基因座的基因组编辑的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸，以及包含所述多核苷酸和融合蛋白的细胞。
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公开(公告)号：US20100218264A1
公开(公告)日：2010-08-26
申请号：US12592852
申请日：2009-12-03
申请人： Xiaoxia Cui , Aron M. Geurts , Fyodor Urnov
发明人： Xiaoxia Cui , Aron M. Geurts , Fyodor Urnov
IPC分类号： A01K67/027 , C12N15/87
CPC分类号： C12N15/907 , A01K67/0275 , A01K67/0276 , A01K67/0278 , A01K2217/054 , A01K2217/15 , A01K2227/10 , A01K2227/105 , A01K2267/03 , A01K2267/0393 , C07K2319/00 , C07K2319/81 , C12N9/22 , C12N15/1024 , C12N15/8509 , C12N2015/8527 , C12N2800/80
摘要： Disclosed herein are methods and compositions for genome editing of one or more loci in a rat, using fusion proteins comprising a zinc-finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
摘要翻译： 本文公开了使用包含锌指蛋白和切割结构域或裂解半结构域的融合蛋白进行大鼠中一个或多个基因座的基因组编辑的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸，以及包含所述多核苷酸和融合蛋白的细胞。
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公开(公告)号：US09255259B2
公开(公告)日：2016-02-09
申请号：US12931760
申请日：2011-02-09
申请人： Gregory J. Cost , Dmitry M. Guschin , Fyodor Urnov
发明人： Gregory J. Cost , Dmitry M. Guschin , Fyodor Urnov
CPC分类号： C12N15/902 , C07K2319/80 , C07K2319/81 , C12N9/22 , C12N15/907
摘要： Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
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公开(公告)号：US08329986B2
公开(公告)日：2012-12-11
申请号：US12653735
申请日：2009-12-17
申请人： Holly J. Butler , David R. Corbin , Yannick Doyon , Zhifang Gao , Vipula K. Shukla , Fyodor Urnov , Sarah E. Worden
发明人： Holly J. Butler , David R. Corbin , Yannick Doyon , Zhifang Gao , Vipula K. Shukla , Fyodor Urnov , Sarah E. Worden
CPC分类号： C12N15/8213
摘要： Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into a plant Zp15 locus, for example, for expression of a polypeptide of interest.
摘要翻译： 本文公开了用于将外源序列靶向整合到植物Zp15基因座中的方法和组合物，例如用于表达感兴趣的多肽。
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公开(公告)号：US20120142055A1
公开(公告)日：2012-06-07
申请号：US13341228
申请日：2011-12-30
CPC分类号： C12N15/907 , C07K14/435 , C07K14/43595 , C07K2319/81 , C12N9/22 , C12N15/1082 , C12N15/113 , C12N2800/10 , C12N2800/30 , C12Y301/21004
摘要： Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest.
摘要翻译： 本文公开了用于将外源序列靶向整合到人PPP1R12C基因座中的方法和组合物，例如用于表达感兴趣的多肽。
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公开(公告)号：US20120060230A1
公开(公告)日：2012-03-08
申请号：US13188417
申请日：2011-07-21
申请人： Trevor Collingwood , Laurence J.N. Cooper , Philip D. Gregory , Michael C. Holmes , Jeffrey C. Miller , Edward J. Rebar , Andreas Reik , Fyodor Urnov
发明人： Trevor Collingwood , Laurence J.N. Cooper , Philip D. Gregory , Michael C. Holmes , Jeffrey C. Miller , Edward J. Rebar , Andreas Reik , Fyodor Urnov
IPC分类号： A61K48/00 , C12N15/11 , A61P5/38 , A61P19/00 , A61P37/00 , A61P7/02 , A61P3/00 , A61P31/14 , A61P5/14 , A61P29/00 , A61P17/06 , A61P19/02 , A61P17/00 , A61P19/04 , C12N9/96 , C12N15/63 , C12N1/19 , C12N5/10 , C12N5/071 , C12N5/0775 , C12N5/0735 , C12N5/0797 , A01K67/027 , C07K7/06
CPC分类号： C07K14/70539 , A61K35/17 , C07K2319/81 , C12N15/907 , C12N2800/80
摘要： Disclosed herein are methods and compositions for modulating the expression of a HLA locus or for selectively deleting or manipulating a HLA locus or HLA regulator.
摘要翻译： 本文公开了用于调节HLA基因座表达或选择性删除或操纵HLA基因座或HLA调节子的方法和组合物。
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公开(公告)号：US07923542B2
公开(公告)日：2011-04-12
申请号：US10083682
申请日：2001-10-24
发明人： Alan P. Wolffe , Fyodor Urnov
CPC分类号： G06F19/22 , C07K14/4702 , C07K2319/00 , C07K2319/02 , C07K2319/09 , C07K2319/24 , C07K2319/43 , C07K2319/71 , C07K2319/75 , C07K2319/81 , C12N15/102 , C12N15/1034 , C12N15/62 , C12N15/63 , C12N15/66 , C12N15/67 , C12Q1/6811 , G06F19/28 , C12Q2531/113 , C12Q2525/191 , C12Q2522/101
摘要： Methods and compositions for the identification, isolation and characterization of regulatory DNA sequences in a cell of interest are provided. In particular, libraries of regulatory sequences are provided, in which each member of the library comprises a polynucleotide comprising sequences from an accessible region of cellular chromatin.
摘要翻译： 提供了用于鉴定，分离和鉴定感兴趣的细胞中调节DNA序列的方法和组合物。 特别地，提供了调节序列的文库，其中文库的每个成员包含包含来自细胞染色质的可及区域的序列的多核苷酸。
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公开(公告)号：US07785792B2
公开(公告)日：2010-08-31
申请号：US12217953
申请日：2008-07-10
申请人： Alan P. Wolffe , Elizabeth J. Wolffe, legal representative , Trevor Collingwood , Philip D. Gregory , Andrew Snowden , Fyodor Urnov
CPC分类号： C12N15/1058 , C07K14/4702 , C07K2319/00 , C07K2319/02 , C07K2319/09 , C07K2319/24 , C07K2319/43 , C07K2319/71 , C07K2319/75 , C07K2319/81 , C12N15/102 , C12N15/1034 , C12N15/62 , C12N15/63 , C12N15/66 , C12N15/67 , C12Q1/6811 , G06F19/22 , G06F19/28
摘要： Methods and compositions for targeted modification of chromatin structure, within a region of interest in cellular chromatin, are provided. Such methods and compositions are useful for facilitating processes such as, for example, transcription and recombination, that require access of exogenous molecules to chromosomal DNA sequences.
摘要翻译： 提供了在细胞染色质感兴趣的区域内靶向修饰染色质结构的方法和组合物。 这些方法和组合物可用于促进需要外源分子进入染色体DNA序列的过程，例如转录和重组。
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公开(公告)号：US20060166206A1
公开(公告)日：2006-07-27
申请号：US10533847
申请日：2003-11-17
申请人： Fyodor Urnov , Eric Rhodes
发明人： Fyodor Urnov , Eric Rhodes
CPC分类号： C12Q1/6837 , C12N15/1034
摘要： Methods for constructing arrays of regulatory sequences, and the arrays so obtained, are provided. Regulatory sequences for use on the arrays are isolated based on their accessibility in cellular chromatin. A number of methods for using the arrays are disclosed, including regulatory DNA profiling, epigenome profiling, toxicological profiling and identification of in vivo binding sites of DNA binding proteins in complex genomes.
摘要翻译： 提供了构建调节序列阵列的方法以及如此得到的阵列。 基于其在细胞染色质中的可及性，分离用于阵列的调节序列。 公开了许多使用阵列的方法，包括调节DNA分析，表观基因组分析，毒理学分析和鉴定复合基因组中DNA结合蛋白的体内结合位点。
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公开(公告)号：US20050032108A1
公开(公告)日：2005-02-10
申请号：US10922546
申请日：2004-08-19
申请人： Casey Case , Fyodor Urnov
发明人： Casey Case , Fyodor Urnov
IPC分类号： C12N15/09 , C12N15/10 , C12N15/12 , C12N15/82 , C12Q1/68 , G01N33/50 , G01N33/48 , G06F19/00
CPC分类号： C12N15/1055 , C12N15/1079 , C12N15/8216 , C12N15/8241 , C12N15/8243 , C12Q1/6897 , G01N33/50 , G01N33/5005
摘要： The present disclosure provides methods and compositions for identifying transcribed regions in a genome. The methods include the use of exogenous molecules such as zinc finger proteins which are capable of binding to and modulating transcription, followed by assay for one or more selected phenotypes.
摘要翻译： 本公开提供用于鉴定基因组中转录区的方法和组合物。 所述方法包括使用能够结合和调节转录的外来分子例如锌指蛋白，随后测定一种或多种选择的表型。
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