公开(公告)号：US09101547B2

公开(公告)日：2015-08-11

申请号：US13561105

申请日：2012-07-30

申请人： Yu Qian ,  Li Juan Zhang ,  Zhi Min Wu ,  Li Ying Zhou ,  Wei Jiang ,  Li Ling ,  Qian Luo ,  Xin Dong Guo

发明人： Yu Qian ,  Li Juan Zhang ,  Zhi Min Wu ,  Li Ying Zhou ,  Wei Jiang ,  Li Ling ,  Qian Luo ,  Xin Dong Guo

IPC分类号： A61K9/50 ,  A61K38/28 ,  A61K9/16 ,  A61K9/48 ,  B82Y5/00

CPC分类号： A61K9/5026 ,  A61K9/1635 ,  A61K9/1647 ,  A61K9/4891 ,  A61K38/28 ,  B82Y5/00

摘要： The invention relates to an enteric-coated capsule containing cationic nanoparticles for oral insulin delivery, in particular to a type of cationic nanoparticle including a polycationic and mucoadhesive polymer and a biodegradable polymer, wherein each of the nanoparticles has positive surface charge and enhanced permeability for paracellular insulin delivery; the enteric-coated capsule further includes a pH-sensitive polymer as the coating. The enteric-coated capsule containing cationic nanoparticles, when being orally administered to a subject, are configured to prevent the acidic degradation of the active substance such as insulin before being released from said cationic nanoparticles to a specific absorption site along the gastrointestinal tract.

摘要翻译： 本发明涉及含有用于口服胰岛素递送的阳离子纳米颗粒的肠溶衣胶囊，特别涉及一种阳离子纳米颗粒，包括聚阳离子和粘膜粘附聚合物和可生物降解的聚合物，其中每种纳米颗粒具有正表面电荷并且增加细胞旁通道的渗透性 胰岛素分泌 肠溶衣胶囊还包括作为涂层的pH敏感聚合物。 包含阳离子纳米颗粒的肠溶衣胶囊当被口服给药时被配置成防止活性物质如胰岛素在从所述阳离子纳米颗粒释放到沿着胃肠道的特定吸收部位之前的酸性降解。

公开(公告)号：US08859004B2

公开(公告)日：2014-10-14

申请号：US13561106

申请日：2012-07-30

申请人： Lijuan Zhang ,  Li Ling ,  Li Ying Zhou ,  Zhi Min Wu ,  Xin Dong Guo ,  Wei Jiang ,  Qian Luo ,  Yu Qian

发明人： Lijuan Zhang ,  Li Ling ,  Li Ying Zhou ,  Zhi Min Wu ,  Xin Dong Guo ,  Wei Jiang ,  Qian Luo ,  Yu Qian

IPC分类号： A61K9/51 ,  A61K38/28

CPC分类号： A61K9/5153 ,  A61K9/5161 ,  A61K9/5192 ,  A61K38/28 ,  Y10S977/906

摘要： The present invention discloses the pH-sensitive nanoparticles composed of pH-sensitive polymer, hydrophobic material, internal stabilizer, external stabilizer content and insulin drug. The present invention also includes a method for preparation of pH-sensitive nanoparticles, in particular, a multiple emulsions solvent evaporation method. The pH-sensitive nanoparticles of the present invention show good pH-sensitive property with 100-300 nanometer particle size. Significant decrease in blood glucose level is observed in streptozotocin (STZ)-induced diabetic rats and the bioavailability of insulin is more than 10% after oral administration of the insulin-loaded pH-sensitive nanoparticles.

摘要翻译： 本发明公开了由pH敏感聚合物，疏水材料，内部稳定剂，外部稳定剂含量和胰岛素药物组成的pH敏感纳米颗粒。 本发明还包括用于制备pH敏感性纳米颗粒的方法，特别是多重乳液溶剂蒸发法。 本发明的pH敏感纳米颗粒具有100-300纳米粒度的良好的pH敏感性质。 在链脲佐菌素（STZ）诱导的糖尿病大鼠中观察到血糖水平的显着降低，并且口服施用负载胰岛素的pH敏感纳米颗粒后胰岛素的生物利用度大于10％。

公开(公告)号：US20100096273A1

公开(公告)日：2010-04-22

申请号：US12256418

申请日：2008-10-22

申请人： Qian Luo ,  Arvind Sundarrajan ,  Hua Chung ,  Xianmin Tang ,  Jick M. Yu ,  Murali K. Narasimhan

发明人： Qian Luo ,  Arvind Sundarrajan ,  Hua Chung ,  Xianmin Tang ,  Jick M. Yu ,  Murali K. Narasimhan

IPC分类号： H01L21/288

CPC分类号： H01L21/76873 ,  C25D5/02 ,  C25D21/12 ,  H01L21/76843 ,  H01L21/76856 ,  H01L21/76862 ,  H01L21/76864 ,  H01L21/76877

摘要： A method and apparatus for selectively controlling deposition rate of conductive material during an electroplating process. Dopants are predominantly incorporated into a conductive seed layer on field regions of a substrate prior to filling openings in the field regions by electroplating. A substrate is positioned in one or more processing chambers, and barrier and conductive seed layers formed. A dopant precursor is provided to the chamber and ionized, with or without voltage bias. The dopant predominantly incorporates into the conductive seed layer on the field regions. Electrical conductivity of the conductive seed layer on the field regions is reduced relative to that of the conductive seed layer in the openings, resulting in low initial deposition rate of metal on the field regions during electroplating, and little or no void formation in the metal deposited in the openings.

摘要翻译： 一种用于在电镀过程中选择性地控制导电材料的沉积速率的方法和装置。 在通过电镀在场区域中填充开口之前，掺杂剂主要被结合到衬底的场区域上的导电种子层中。 衬底被定位在一个或多个处理室中，形成阻挡层和导电种子层。 在室内提供掺杂剂前体，并且在电压偏置或没有电压偏置的情况下电离。 掺杂剂主要并入到场区域上的导电种子层中。 导电种子层在场区域的电导率相对于开口中的导电种子层的导电率降低，导致电镀期间金属在场区域上的初始沉积速率较低，并且金属沉积中几乎没有或没有空隙形成 在开口。

公开(公告)号：US20070207224A1

公开(公告)日：2007-09-06

申请号：US11622468

申请日：2007-01-12

申请人： Ka Ming NG ,  Qian Luo ,  Jingnan Zhang ,  Benny Harjo

发明人： Ka Ming NG ,  Qian Luo ,  Jingnan Zhang ,  Benny Harjo

IPC分类号： A61K36/48 ,  A61K31/353

CPC分类号： A61K36/48 ,  A61K31/353

摘要： This invention relates to a novel soy isoflavone product with high purities and strong biological activities and the method of producing the same from natural soybeans, soybean materials (i.e. tofu dregs, soy molasses) and other plant sources. The method includes three steps consisting of extraction with an organic solvent, hydrolysis using an acid and crystallization using an antisolvent. The procedure is very simple and thus can be easily adapted for large-scale manufacturing. Moreover, the procedure is able to produce a high yield of total isoflavones at a lower cost. HPLC analysis and E-Screen bioassay reveal that the obtained product not only contains a high content of isoflavone aglycones by weight of dry matter but also exhibits strong estrogenic activity toward human cells. Therefore, the product should be efficacious for relieving menopausal symptoms and other estrogen-deficient diseases and can be used in health care supplements or as additives for foods, beverages or cosmetics.

摘要翻译： 本发明涉及一种具有高纯度和强生物活性的新型大豆异黄酮产品及其生产方法，包括天然大豆，大豆原料（即豆腐渣，大豆糖蜜）和其他植物来源。 该方法包括用有机溶剂萃取，使用酸水解和使用反溶剂进行结晶的三个步骤。 该程序非常简单，因此可以轻松适应大规模制造。 此外，该方法能够以更低的成本产生高产量的总异黄酮。 HPLC分析和E-Screen生物测定显示，所得产物不仅含有高含量的异黄酮糖苷配基，其重量为干物质，而且对人细胞表现出强烈的雌激素活性。 因此，该产品应有效减轻绝经症状和其他雌激素缺乏症，可用于保健品或食品，饮料或化妆品的添加剂。

公开(公告)号：US07049400B1

公开(公告)日：2006-05-23

申请号：US09551380

申请日：2000-04-18

申请人： Donald Choy Chang ,  Qian Luo

发明人： Donald Choy Chang ,  Qian Luo

IPC分类号： C07K1/00 ,  C12N9/00 ,  C12N9/50 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12Q1/37 ,  C07K14/43595 ,  G01N33/582 ,  G01N2333/96466 ,  G01N2510/00

摘要： The present invention concerns fluorescent proteins modified such that said modified fluorescent protein incorporates a cleavage site for a protease, cleavage of said modified fluorescent protein at said cleavage site by said protease causing the alteration of at least one of the emission and excitation spectra of said modified fluorescent protein. In particular, the invention is concerned with using these modified fluorescent proteins as probes for detecting protease activity in living cells during the programmed cell death process (apoptosis).Also provided are nucleic acid sequence encoding same, recombinant DNA constructs expressing same, cells transformed or transfected with same, methods for detecting protease activity, and methods of detecting agents which affect protease activity, and kits for same.

摘要翻译： 本发明涉及修饰的荧光蛋白，使得所述修饰的荧光蛋白掺入蛋白酶的切割位点，所述蛋白酶在所述切割位点切割所述修饰的荧光蛋白，导致所述修饰的发射和激发光谱中的至少一个的改变 荧光蛋白。 特别地，本发明涉及使用这些修饰的荧光蛋白作为在程序性细胞死亡过程（细胞凋亡）期间检测活细胞中的蛋白酶活性的探针。 还提供了编码其的核酸序列，表达相同的重组DNA构建体，用其转化或转染的细胞，检测蛋白酶活性的方法，以及检测影响蛋白酶活性的试剂的方法，以及用于其的试剂盒。

公开(公告)号：US07553505B2

公开(公告)日：2009-06-30

申请号：US11622468

申请日：2007-01-12

申请人： Ka Ming Ng ,  Qian Luo ,  Jingnan Zhang ,  Benny Harjo

发明人： Ka Ming Ng ,  Qian Luo ,  Jingnan Zhang ,  Benny Harjo

IPC分类号： A61K36/48

CPC分类号： A61K36/48 ,  A61K31/353

摘要： This invention relates to a novel soy isoflavone product with high purities and strong biological activities and the method of producing the same from natural soybeans, soybean materials (i.e. tofu dregs, soy molasses) and other plant sources. The method includes three steps consisting of extraction with an organic solvent, hydrolysis using an acid and crystallization using an antisolvent. The procedure is very simple and thus can be easily adapted for large-scale manufacturing. Moreover, the procedure is able to produce a high yield of total isoflavones at a lower cost. HPLC analysis and E-Screen bioassay reveal that the obtained product not only contains a high content of isoflavone aglycones by weight of dry matter but also exhibits strong estrogenic activity toward human cells. Therefore, the product should be efficacious for relieving menopausal symptoms and other estrogen-deficient diseases and can be used in health care supplements or as additives for foods, beverages or cosmetics.

摘要翻译： 本发明涉及一种具有高纯度和强生物活性的新型大豆异黄酮产品及其生产方法，包括天然大豆，大豆原料（即豆腐渣，大豆糖蜜）和其他植物来源。 该方法包括用有机溶剂萃取，使用酸水解和使用反溶剂进行结晶的三个步骤。 该程序非常简单，因此可以轻松适应大规模制造。 此外，该方法能够以更低的成本产生高产量的总异黄酮。 HPLC分析和E-Screen生物测定显示，所得产物不仅含有高含量的异黄酮糖苷配基，其重量为干物质，而且对人细胞表现出强烈的雌激素活性。 因此，该产品应有效减轻绝经症状和其他雌激素缺乏症，可用于保健品或食品，饮料或化妆品的添加剂。

公开(公告)号：US08764961B2

公开(公告)日：2014-07-01

申请号：US12256418

申请日：2008-10-22

申请人： Qian Luo ,  Arvind Sundarrajan ,  Hua Chung ,  Xianmin Tang ,  Jick M. Yu ,  Murali K. Narasimhan

发明人： Qian Luo ,  Arvind Sundarrajan ,  Hua Chung ,  Xianmin Tang ,  Jick M. Yu ,  Murali K. Narasimhan

IPC分类号： C25D5/34 ,  C25D7/12

CPC分类号： H01L21/76873 ,  C25D5/02 ,  C25D21/12 ,  H01L21/76843 ,  H01L21/76856 ,  H01L21/76862 ,  H01L21/76864 ,  H01L21/76877

摘要： A method and apparatus for selectively controlling deposition rate of conductive material during an electroplating process. Dopants are predominantly incorporated into a conductive seed layer on field regions of a substrate prior to filling openings in the field regions by electroplating. A substrate is positioned in one or more processing chambers, and barrier and conductive seed layers formed. A dopant precursor is provided to the chamber and ionized, with or without voltage bias. The dopant predominantly incorporates into the conductive seed layer on the field regions. Electrical conductivity of the conductive seed layer on the field regions is reduced relative to that of the conductive seed layer in the openings, resulting in low initial deposition rate of metal on the field regions during electroplating, and little or no void formation in the metal deposited in the openings.

摘要翻译： 一种用于在电镀过程中选择性地控制导电材料的沉积速率的方法和装置。 在通过电镀在场区域中填充开口之前，掺杂剂主要被结合到衬底的场区域上的导电种子层中。 衬底被定位在一个或多个处理室中，形成阻挡层和导电种子层。 在室内提供掺杂剂前体，并且在电压偏置或没有电压偏置的情况下电离。 掺杂剂主要并入到场区域上的导电种子层中。 导电种子层在场区域的电导率相对于开口中的导电种子层的导电率降低，导致电镀期间金属在场区域上的初始沉积速率较低，并且金属沉积中几乎没有或没有空隙形成 在开口。

公开(公告)号：US20080190760A1

公开(公告)日：2008-08-14

申请号：US11838796

申请日：2007-08-14

申请人： XIANMIN TANG ,  Arvind Sundarrajan ,  Daniel Lubben ,  Qian Luo ,  Tza-Jing Gung ,  Anantha Subramani ,  Hua Chung ,  Xinyu Fu ,  Rongjun Wang ,  Yong Cao ,  Jick Yu ,  John Forster ,  Praburam Gopalraja

发明人： XIANMIN TANG ,  Arvind Sundarrajan ,  Daniel Lubben ,  Qian Luo ,  Tza-Jing Gung ,  Anantha Subramani ,  Hua Chung ,  Xinyu Fu ,  Rongjun Wang ,  Yong Cao ,  Jick Yu ,  John Forster ,  Praburam Gopalraja

IPC分类号： C23C14/32

CPC分类号： C23C14/165 ,  C23C14/046 ,  C23C14/358 ,  C23C14/5826 ,  C23C14/584 ,  H01J37/3408 ,  H01J37/3455 ,  H01L21/2855 ,  H01L21/76865 ,  H01L21/76873 ,  H01L21/76877

摘要： An integrated copper deposition process, particularly useful for forming a copper seed layer in a narrow via prior to electrochemical plating of copper, including at least one cycle of sputter deposition of copper followed by sputter etching of the deposited copper, preferably performed in a same sputter chamber. The deposition is performed under conditions promoting high copper ionization fractions and strong wafer biasing to draw the copper ions into the via. The etching may be done with argon ions, preferably inductively excited by an RF coil around the chamber, or by copper ions, which may be formed with high target power and intense magnetron or by use of the RF coil. Two or more cycles of deposition/etch may be performed. A final flash deposition may be performed with high copper ionization and low wafer biasing.

摘要翻译： 一种集成的铜沉积工艺，特别适用于在铜的电化学电镀之前在窄通孔中形成铜籽晶层，包括至少一个铜的溅射沉积循环，然后溅射蚀刻沉积的铜，优选在相同的溅射 房间。 沉积在促进高铜离子化分数和强晶片偏置的条件下进行以将铜离子吸入通孔。 蚀刻可以用氩离子进行，优选地通过围绕室的RF线圈进行感应激励，或者通过可以用高目标功率和强磁控管形成的铜离子或通过使用RF线圈进行。 可以执行两个或更多个沉积/蚀刻循环。 最终的闪蒸沉积可以用高铜离子化和低晶片偏压进行。