公开(公告)号：US08859004B2

公开(公告)日：2014-10-14

申请号：US13561106

申请日：2012-07-30

申请人： Lijuan Zhang ,  Li Ling ,  Li Ying Zhou ,  Zhi Min Wu ,  Xin Dong Guo ,  Wei Jiang ,  Qian Luo ,  Yu Qian

发明人： Lijuan Zhang ,  Li Ling ,  Li Ying Zhou ,  Zhi Min Wu ,  Xin Dong Guo ,  Wei Jiang ,  Qian Luo ,  Yu Qian

IPC分类号： A61K9/51 ,  A61K38/28

CPC分类号： A61K9/5153 ,  A61K9/5161 ,  A61K9/5192 ,  A61K38/28 ,  Y10S977/906

摘要： The present invention discloses the pH-sensitive nanoparticles composed of pH-sensitive polymer, hydrophobic material, internal stabilizer, external stabilizer content and insulin drug. The present invention also includes a method for preparation of pH-sensitive nanoparticles, in particular, a multiple emulsions solvent evaporation method. The pH-sensitive nanoparticles of the present invention show good pH-sensitive property with 100-300 nanometer particle size. Significant decrease in blood glucose level is observed in streptozotocin (STZ)-induced diabetic rats and the bioavailability of insulin is more than 10% after oral administration of the insulin-loaded pH-sensitive nanoparticles.

摘要翻译： 本发明公开了由pH敏感聚合物，疏水材料，内部稳定剂，外部稳定剂含量和胰岛素药物组成的pH敏感纳米颗粒。 本发明还包括用于制备pH敏感性纳米颗粒的方法，特别是多重乳液溶剂蒸发法。 本发明的pH敏感纳米颗粒具有100-300纳米粒度的良好的pH敏感性质。 在链脲佐菌素（STZ）诱导的糖尿病大鼠中观察到血糖水平的显着降低，并且口服施用负载胰岛素的pH敏感纳米颗粒后胰岛素的生物利用度大于10％。

公开(公告)号：US20130034589A1

公开(公告)日：2013-02-07

申请号：US13561106

申请日：2012-07-30

申请人： Lijuan ZHANG ,  Li LING ,  Li Ying ZHOU ,  Zhi Min WU ,  Xin Dong GUO ,  Wei JIANG ,  Qian LUO ,  Yu QIAN

发明人： Lijuan ZHANG ,  Li LING ,  Li Ying ZHOU ,  Zhi Min WU ,  Xin Dong GUO ,  Wei JIANG ,  Qian LUO ,  Yu QIAN

IPC分类号： A61K9/14 ,  A61K38/28 ,  A61P3/10 ,  B82Y40/00 ,  B82Y5/00

CPC分类号： A61K9/5153 ,  A61K9/5161 ,  A61K9/5192 ,  A61K38/28 ,  Y10S977/906

摘要： The present invention discloses the pH-sensitive nanoparticles composed of pH-sensitive polymer, hydrophobic material, internal stabilizer, external stabilizer content and insulin drug. The present invention also includes a method for preparation of pH-sensitive nanoparticles, in particular, a multiple emulsions solvent evaporation method. The pH-sensitive nanoparticles of the present invention show good pH-sensitive property with 100-300 nanometer particle size. Significant decrease in blood glucose level is observed in streptozotocin (STZ)-induced diabetic rats and the bioavailability of insulin is more than 10% after oral administration of the insulin-loaded pH-sensitive nanoparticles.

摘要翻译： 本发明公开了由pH敏感聚合物，疏水材料，内部稳定剂，外部稳定剂含量和胰岛素药物组成的pH敏感纳米颗粒。 本发明还包括用于制备pH敏感性纳米颗粒的方法，特别是多重乳液溶剂蒸发法。 本发明的pH敏感纳米颗粒具有100-300纳米粒度的良好的pH敏感性质。 在链脲佐菌素（STZ）诱导的糖尿病大鼠中观察到血糖水平的显着降低，并且口服施用负载胰岛素的pH敏感纳米颗粒后胰岛素的生物利用度大于10％。

公开(公告)号：US20100096273A1

公开(公告)日：2010-04-22

申请号：US12256418

申请日：2008-10-22

申请人： Qian Luo ,  Arvind Sundarrajan ,  Hua Chung ,  Xianmin Tang ,  Jick M. Yu ,  Murali K. Narasimhan

发明人： Qian Luo ,  Arvind Sundarrajan ,  Hua Chung ,  Xianmin Tang ,  Jick M. Yu ,  Murali K. Narasimhan

IPC分类号： H01L21/288

CPC分类号： H01L21/76873 ,  C25D5/02 ,  C25D21/12 ,  H01L21/76843 ,  H01L21/76856 ,  H01L21/76862 ,  H01L21/76864 ,  H01L21/76877

摘要： A method and apparatus for selectively controlling deposition rate of conductive material during an electroplating process. Dopants are predominantly incorporated into a conductive seed layer on field regions of a substrate prior to filling openings in the field regions by electroplating. A substrate is positioned in one or more processing chambers, and barrier and conductive seed layers formed. A dopant precursor is provided to the chamber and ionized, with or without voltage bias. The dopant predominantly incorporates into the conductive seed layer on the field regions. Electrical conductivity of the conductive seed layer on the field regions is reduced relative to that of the conductive seed layer in the openings, resulting in low initial deposition rate of metal on the field regions during electroplating, and little or no void formation in the metal deposited in the openings.

摘要翻译： 一种用于在电镀过程中选择性地控制导电材料的沉积速率的方法和装置。 在通过电镀在场区域中填充开口之前，掺杂剂主要被结合到衬底的场区域上的导电种子层中。 衬底被定位在一个或多个处理室中，形成阻挡层和导电种子层。 在室内提供掺杂剂前体，并且在电压偏置或没有电压偏置的情况下电离。 掺杂剂主要并入到场区域上的导电种子层中。 导电种子层在场区域的电导率相对于开口中的导电种子层的导电率降低，导致电镀期间金属在场区域上的初始沉积速率较低，并且金属沉积中几乎没有或没有空隙形成 在开口。

公开(公告)号：US20120024229A1

公开(公告)日：2012-02-02

申请号：US13195171

申请日：2011-08-01

申请人： GUOJUN LIU ,  XIANMIN TANG ,  QIAN LUO ,  YONG CAO

发明人： GUOJUN LIU ,  XIANMIN TANG ,  QIAN LUO ,  YONG CAO

IPC分类号： C23C16/511 ,  H01F7/02

CPC分类号： H04M3/58 ,  H01F7/0278 ,  H01J37/3408 ,  H01J37/3461 ,  H04L65/1016 ,  H04M2207/18 ,  H04W48/20 ,  H04W60/00 ,  H04W76/10

摘要： Magnetrons for use in physical vapor deposition (PVD) chambers and methods of use thereof are provided herein. In some embodiments, an apparatus may include a support member having an axis of rotation; a plurality of first magnets coupled to the support member on a first side of the axis of rotation and having a first polarity oriented in a first direction perpendicular to the support member; and a second magnet coupled to the support member on a second side of the axis of rotation opposite the first side and having a second polarity oriented in a second direction opposite the first direction. In some embodiments, the apparatus is capable of forming a magnetic field including one or more magnetic nulls that modulate local plasma uniformity in a physical vapor deposition (PVD) chamber.

摘要翻译： 本文提供了用于物理气相沉积（PVD）室的磁控管及其使用方法。 在一些实施例中，装置可以包括具有旋转轴线的支撑构件; 多个第一磁体，其在所述旋转轴线的第一侧上联接到所述支撑构件，并且具有在垂直于所述支撑构件的第一方向上定向的第一极性; 以及第二磁体，其在所述旋转轴线的与所述第一侧相反的第二侧上与所述支撑构件相耦合，并且具有在与所述第一方向相反的第二方向上定向的第二极性。 在一些实施例中，该装置能够形成包括调制物理气相沉积（PVD）室中的局部等离子体均匀性的一个或多个磁零点的磁场。

公开(公告)号：US20070207224A1

公开(公告)日：2007-09-06

申请号：US11622468

申请日：2007-01-12

申请人： Ka Ming NG ,  Qian Luo ,  Jingnan Zhang ,  Benny Harjo

发明人： Ka Ming NG ,  Qian Luo ,  Jingnan Zhang ,  Benny Harjo

IPC分类号： A61K36/48 ,  A61K31/353

CPC分类号： A61K36/48 ,  A61K31/353

摘要： This invention relates to a novel soy isoflavone product with high purities and strong biological activities and the method of producing the same from natural soybeans, soybean materials (i.e. tofu dregs, soy molasses) and other plant sources. The method includes three steps consisting of extraction with an organic solvent, hydrolysis using an acid and crystallization using an antisolvent. The procedure is very simple and thus can be easily adapted for large-scale manufacturing. Moreover, the procedure is able to produce a high yield of total isoflavones at a lower cost. HPLC analysis and E-Screen bioassay reveal that the obtained product not only contains a high content of isoflavone aglycones by weight of dry matter but also exhibits strong estrogenic activity toward human cells. Therefore, the product should be efficacious for relieving menopausal symptoms and other estrogen-deficient diseases and can be used in health care supplements or as additives for foods, beverages or cosmetics.

摘要翻译： 本发明涉及一种具有高纯度和强生物活性的新型大豆异黄酮产品及其生产方法，包括天然大豆，大豆原料（即豆腐渣，大豆糖蜜）和其他植物来源。 该方法包括用有机溶剂萃取，使用酸水解和使用反溶剂进行结晶的三个步骤。 该程序非常简单，因此可以轻松适应大规模制造。 此外，该方法能够以更低的成本产生高产量的总异黄酮。 HPLC分析和E-Screen生物测定显示，所得产物不仅含有高含量的异黄酮糖苷配基，其重量为干物质，而且对人细胞表现出强烈的雌激素活性。 因此，该产品应有效减轻绝经症状和其他雌激素缺乏症，可用于保健品或食品，饮料或化妆品的添加剂。

公开(公告)号：US07049400B1

公开(公告)日：2006-05-23

申请号：US09551380

申请日：2000-04-18

申请人： Donald Choy Chang ,  Qian Luo

发明人： Donald Choy Chang ,  Qian Luo

IPC分类号： C07K1/00 ,  C12N9/00 ,  C12N9/50 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12Q1/37 ,  C07K14/43595 ,  G01N33/582 ,  G01N2333/96466 ,  G01N2510/00

摘要： The present invention concerns fluorescent proteins modified such that said modified fluorescent protein incorporates a cleavage site for a protease, cleavage of said modified fluorescent protein at said cleavage site by said protease causing the alteration of at least one of the emission and excitation spectra of said modified fluorescent protein. In particular, the invention is concerned with using these modified fluorescent proteins as probes for detecting protease activity in living cells during the programmed cell death process (apoptosis).Also provided are nucleic acid sequence encoding same, recombinant DNA constructs expressing same, cells transformed or transfected with same, methods for detecting protease activity, and methods of detecting agents which affect protease activity, and kits for same.

摘要翻译： 本发明涉及修饰的荧光蛋白，使得所述修饰的荧光蛋白掺入蛋白酶的切割位点，所述蛋白酶在所述切割位点切割所述修饰的荧光蛋白，导致所述修饰的发射和激发光谱中的至少一个的改变 荧光蛋白。 特别地，本发明涉及使用这些修饰的荧光蛋白作为在程序性细胞死亡过程（细胞凋亡）期间检测活细胞中的蛋白酶活性的探针。 还提供了编码其的核酸序列，表达相同的重组DNA构建体，用其转化或转染的细胞，检测蛋白酶活性的方法，以及检测影响蛋白酶活性的试剂的方法，以及用于其的试剂盒。

公开(公告)号：US09226912B2

公开(公告)日：2016-01-05

申请号：US14009431

申请日：2012-02-13

申请人： Kathy Qian Luo ,  Ting Yu ,  Hou-Wei Luo

发明人： Kathy Qian Luo ,  Ting Yu ,  Hou-Wei Luo

IPC分类号： A61K31/343 ,  C07D307/77 ,  C07D307/92 ,  C07D253/00 ,  A61K31/337 ,  A61K45/06

CPC分类号： A61K31/343 ,  A61K31/337 ,  A61K45/06 ,  C07D253/00 ,  C07D307/77 ,  C07D307/92

摘要： The present invention relates to the use of a compound of Formula I for the manufacture of a medicament for treating or preventing cancer in a subject. Formula I

摘要翻译： 本发明涉及式I化合物在制备用于治疗或预防受试者癌症的药物中的用途。 一级方程式

公开(公告)号：US09101547B2

公开(公告)日：2015-08-11

申请号：US13561105

申请日：2012-07-30

申请人： Yu Qian ,  Li Juan Zhang ,  Zhi Min Wu ,  Li Ying Zhou ,  Wei Jiang ,  Li Ling ,  Qian Luo ,  Xin Dong Guo

发明人： Yu Qian ,  Li Juan Zhang ,  Zhi Min Wu ,  Li Ying Zhou ,  Wei Jiang ,  Li Ling ,  Qian Luo ,  Xin Dong Guo

IPC分类号： A61K9/50 ,  A61K38/28 ,  A61K9/16 ,  A61K9/48 ,  B82Y5/00

CPC分类号： A61K9/5026 ,  A61K9/1635 ,  A61K9/1647 ,  A61K9/4891 ,  A61K38/28 ,  B82Y5/00

摘要： The invention relates to an enteric-coated capsule containing cationic nanoparticles for oral insulin delivery, in particular to a type of cationic nanoparticle including a polycationic and mucoadhesive polymer and a biodegradable polymer, wherein each of the nanoparticles has positive surface charge and enhanced permeability for paracellular insulin delivery; the enteric-coated capsule further includes a pH-sensitive polymer as the coating. The enteric-coated capsule containing cationic nanoparticles, when being orally administered to a subject, are configured to prevent the acidic degradation of the active substance such as insulin before being released from said cationic nanoparticles to a specific absorption site along the gastrointestinal tract.

摘要翻译： 本发明涉及含有用于口服胰岛素递送的阳离子纳米颗粒的肠溶衣胶囊，特别涉及一种阳离子纳米颗粒，包括聚阳离子和粘膜粘附聚合物和可生物降解的聚合物，其中每种纳米颗粒具有正表面电荷并且增加细胞旁通道的渗透性 胰岛素分泌 肠溶衣胶囊还包括作为涂层的pH敏感聚合物。 包含阳离子纳米颗粒的肠溶衣胶囊当被口服给药时被配置成防止活性物质如胰岛素在从所述阳离子纳米颗粒释放到沿着胃肠道的特定吸收部位之前的酸性降解。

公开(公告)号：US20120181166A1

公开(公告)日：2012-07-19

申请号：US13007228

申请日：2011-01-14

申请人： QIAN LUO ,  Ye Xu ,  Tza-Jing Gung ,  Xianmin Tang

发明人： QIAN LUO ,  Ye Xu ,  Tza-Jing Gung ,  Xianmin Tang

IPC分类号： C23C14/35

CPC分类号： C23C14/345 ,  C23C14/3492 ,  C23C14/35 ,  H01J37/3455

摘要： Embodiments of the present invention generally relate to methods for physical vapor deposition processes. The methods generally include synchronizing process chamber conditions with the position of a magnetron. As the magnetron is scanned over a first area of a target, the conditions within the chamber are adjusted to a first set of predetermined process conditions. As the magnetron is subsequently scanned over a second area of the target, the conditions within the chamber are adjusted to a second set of predetermined process conditions different the first set. The target may be divided into more than two areas. By correlating the position of the magnetron with different sets of process conditions, film uniformity can be improved by reducing center-to-edge non-uniformities, such as re-sputter rates which may be higher when the magnetron is near the edge of the target.

摘要翻译： 本发明的实施方案一般涉及物理气相沉积方法的方法。 方法通常包括使处理室条件与磁控管的位置同步。 当磁控管在目标的第一区域上被扫描时，腔室内的条件被调整到第一组预定的工艺条件。 随着磁控管随后在目标的第二区域上扫描，腔室内的条件被调整到与第一组不同的第二组预定过程条件。 目标可分为两个以上的区域。 通过将磁控管的位置与不同的工艺条件相关联，可以通过减小中心到边缘的不均匀性来改善膜均匀性，例如当磁控管靠近靶的边缘时可能更高的再溅射速率 。

公开(公告)号：US20110301236A1

公开(公告)日：2011-12-08

申请号：US12793690

申请日：2010-06-04

申请人： XUE-MEI GAO ,  Ting Yu ,  Kathy Qian Luo ,  Hong Xi Xu

发明人： XUE-MEI GAO ,  Ting Yu ,  Kathy Qian Luo ,  Hong Xi Xu

IPC分类号： A61K31/235 ,  A61P35/00 ,  C07C69/94

CPC分类号： A61K31/235 ,  C07C69/94

摘要： A novel compound isolated from the plant Garcinia paucinervis. The compound's structure is as follows: The compound possesses a potent ability to activate caspase-3 in HeLa-C3 cells within 72 h at a low concentration of 25 μM. The IC50 on HeLa cells for this compound was measured to be below 10 μM, indicating a strong HeLa cell growth inhibitory effect.

摘要翻译： 一种从植物中分离出的新颖化合物。 该化合物的结构如下：该化合物在低浓度25μM下在72小时内具有在HeLa-C3细胞中活化半胱天冬酶-3的有效能力。 测量该化合物对HeLa细胞的IC50低于10μM，表明具有强的HeLa细胞生长抑制作用。