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公开(公告)号：US11618976B2
公开(公告)日：2023-04-04
申请号：US16651232
申请日：2018-10-09
Applicant: SPIBER TECHNOLOGIES AB
Inventor： My Hedhammar , Mathias Kvick , Fredrik Lundell
Abstract: A method for producing a protein polymer fiber, the method comprising providing a liquid protein solution in a container for liquid, and repeatedly moving the liquid surface in the container back and forth between a first and a second position. Said movement of the liquid surface is such that the protein polymer solution is allowed to form a film in the interface between the liquid surface of the liquid protein solution and a surrounding fluid. The movement of the liquid surface being performed by respectively raising and lowering the liquid surface relative to the container or by moving an object extending through the liquid surface of the liquid protein solution. Also, a device for performing said method.
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公开(公告)号：US11484624B2
公开(公告)日：2022-11-01
申请号：US16301819
申请日：2017-05-16
Applicant: SPIBER TECHNOLOGIES AB
Inventor： My Hedhammar , Linnea Nilebäck
Abstract: A method for coating a solid surface with a recombinant spider silk protein capable of forming polymeric, solid structures is provided. The method is comprising the following steps: exposing the solid surface to an aqueous solution of the recombinant spider silk protein and thereby forming a surface layer of the recombinant spider silk protein adsorbed on the solid surface without formation of covalent bonds between the recombinant spider silk protein and the solid surface; and further exposing the surface layer of the solid surface to an aqueous solution of the recombinant spider silk protein and thereby forming an assembled silk structure layer of the recombinant spider silk protein on the surface layer; wherein the method does not include drying-in of spider silk protein.
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公开(公告)号：US11214601B2
公开(公告)日：2022-01-04
申请号：US16816486
申请日：2020-03-12
Applicant: SPIBER TECHNOLOGIES AB
Inventor： Jan Johansson , Anna Rising , Nina Kronqvist , Kerstin Nordling
IPC: C07K14/435 , C07K1/36
Abstract: A protein comprising a moiety of 100-160 amino acid residues having at least 70% identity with the N-terminal (NT) fragment of a spider silk protein, wherein the amino acid residue corresponding to position 40 in NT is selected from the group consisting of Lys, Arg and His; and wherein the amino acid residue corresponding to position 65 in NT is selected from the group consisting of Asp and Glu, is useful as a moiety in a fusion protein for enhancing the solubility of another moiety in the fusion protein, which is a desired protein or polypeptide.
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公开(公告)号：US10604550B2
公开(公告)日：2020-03-31
申请号：US15490328
申请日：2017-04-18
Applicant: SPIBER TECHNOLOGIES AB
Inventor： Jan Johansson , Anna Rising , My Hedhammar , Kerstin Nordling
IPC: C07K14/435 , C12N15/62
Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.
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公开(公告)号：US20190151505A1
公开(公告)日：2019-05-23
申请号：US16301819
申请日：2017-05-16
Applicant: SPIBER TECHNOLOGIES AB
Inventor： My HEDHAMMAR , Linnea NILEBÄCK
Abstract: A method for coating a solid surface with a recombinant spider silk protein capable of forming polymeric, solid structures is provided. The method is comprising the following steps: exposing the solid surface to an aqueous solution of the recombinant spider silk protein and thereby forming a surface layer of the recombinant spider silk protein adsorbed on the solid surface without formation of covalent bonds between the recombinant spider silk protein and the solid surface; and further exposing the surface layer of the solid surface to an aqueous solution of the recombinant spider silk protein and thereby forming an assembled silk structure layer of the recombinant spider silk protein on the surface layer; wherein the method does not include drying-in of spider silk protein.
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Patent Agency Ranking
公开(公告)号：US20180170977A1
公开(公告)日：2018-06-21
申请号：US15739057
申请日：2016-06-23
Applicant: Spiber Technologies AB
Inventor： My Hedhammar
IPC: C07K14/435 , C12N15/62 , C12N5/00 , C08H1/00
CPC classification number: C07K14/43518 , C07K16/2839 , C07K2319/70 , C08H1/00 , C12N5/0068 , C12N15/62 , C12N2533/50
Abstract: A recombinant fusion protein is comprising a spider silk fragment and a cyclic RGD cell-binding motif with selectivity for integrins, such as for α5β1 integrins. The fusion protein is useful as a cell scaffold material and for the cultivation of cells displaying integrins on their cell surface.
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公开(公告)号：US09644012B2
公开(公告)日：2017-05-09
申请号：US13634519
申请日：2010-10-27
Applicant: Jan Johansson , Anna Rising , My Hedhammar , Kerstin Nordling
Inventor： Jan Johansson , Anna Rising , My Hedhammar , Kerstin Nordling
IPC: C07K14/435 , C12N15/62
CPC classification number: C07K14/43518 , C12N15/62
Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.
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8.发明申请METHODS AND COMBINATION COMPRISING EUKARYOTIC CELLS AND RECOMBINANT SPIDER SILK PROTEIN 审中-公开包含真核细胞和重组天花蛋白丝蛋白的方法和组合公开(公告)号：US20160024464A1
公开(公告)日：2016-01-28
申请号：US14840653
申请日：2015-08-31
Applicant: SPIBER TECHNOLOGIES AB
Inventor： Jan JOHANSSON , Anna RISING , My HEDHAMMAR , Ulrika JOHANSSON , Mona WIDHE
IPC: C12N5/00
CPC classification number: C12N5/0068 , A61L27/227 , A61L27/3804 , A61L27/3834 , A61L27/56 , C07K14/005 , C07K14/43518 , C12N5/0606 , C12N5/0623 , C12N5/0629 , C12N5/0647 , C12N5/0656 , C12N5/067 , C12N5/0676 , C12N5/0691 , C12N2500/82 , C12N2533/50 , C12N2539/00 , D01F4/02
Abstract: A method and a combination for the cultivation of eukaryotic cells are provided, as well as a method for preparation of eukaryotic cells. The methods comprise providing a sample of eukaryotic cells to be cultured, applying said sample to a cell scaffold material; and maintaining said cell scaffold material having cells applied thereto under conditions suitable for cell culture. The combination comprises eukaryotic cells and a cell scaffold material. The cell scaffold material comprises a polymer of a spider silk protein.
Abstract translation: 提供了用于培养真核细胞的方法和组合，以及用于制备真核细胞的方法。 所述方法包括提供待培养的真核细胞样品，将所述样品施用于细胞支架材料; 以及在适于细胞培养的条件下保持所述细胞支架材料具有施加于其上的细胞。 该组合包含真核细胞和细胞支架材料。 细胞支架材料包括蜘蛛丝蛋白的聚合物。
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公开(公告)号：US20140079674A1
公开(公告)日：2014-03-20
申请号：US14086244
申请日：2013-11-21
Applicant: Spiber Technologies AB
Inventor： Jan JOHANSSON , Goran HJALM , Margareta STARK , Anna RISING , Stefan GRIP , Wilhelm ENGSTROM , My HEDHAMMAR
IPC: C07K14/435 , A61K47/42 , A61K35/12
CPC classification number: C07K14/43518 , A61K35/12 , A61K47/42 , D01F4/00
Abstract: The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula REP-CT. REP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.
Abstract translation: 本发明提供了一种分离的主要的安瓿蛋白质宿主蛋白，其由150至420个氨基酸残基组成，并由式REP-CT定义。 REP是具有80至300个氨基酸残基的重复的N末端衍生的蛋白质片段。 CT是具有70至120个氨基酸残基的C末端衍生的蛋白质片段。 本发明进一步提供了由第一蛋白片段（其是主要的安非他索特灵素蛋白质）和包含融合配偶体和切割剂识别位点的第二蛋白质片段组成的分离的融合蛋白质。 第一个蛋白质片段通过所述切割剂识别位点连接到融合配偶体。 本发明还提供了一种生产主要的安非他命复合蛋白及其聚合物的方法。
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公开(公告)号：US08173772B2
公开(公告)日：2012-05-08
申请号：US12087289
申请日：2006-12-28
Applicant: Jan Johansson , Göran Hjälm , Margareta Stark , Anna Rising , Stefan Grip , Wilhelm Engström , My Hedhammar
Inventor： Jan Johansson , Göran Hjälm , Margareta Stark , Anna Rising , Stefan Grip , Wilhelm Engström , My Hedhammar
CPC classification number: C07K14/43518 , A61K35/12 , A61K47/42 , D01F4/00
Abstract: The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula KEP-CT. KEP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.
Abstract translation: 本发明提供了一种分离的主要的安瓿蛋白质宿主蛋白，其由150至420个氨基酸残基组成，并由式KEP-CT定义。 KEP是具有80至300个氨基酸残基的重复的N末端衍生的蛋白质片段。 CT是具有70至120个氨基酸残基的C末端衍生的蛋白质片段。 本发明进一步提供了由第一蛋白片段（其是主要的安非他索特灵素蛋白质）和包含融合配偶体和切割剂识别位点的第二蛋白质片段组成的分离的融合蛋白质。 第一个蛋白质片段通过所述切割剂识别位点连接到融合配偶体。 本发明还提供了一种生产主要的安非他命复合蛋白及其聚合物的方法。
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