公开(公告)号：US09656960B2

公开(公告)日：2017-05-23

申请号：US14660453

申请日：2015-03-17

Applicant: Alessandra Bartolozzi ,  Stewart Campbell ,  Hope Foudoulakis ,  Enoch Kim ,  Paul Sweetnam ,  Yingfei Yang

Inventor： Alessandra Bartolozzi ,  Stewart Campbell ,  Hope Foudoulakis ,  Enoch Kim ,  Paul Sweetnam ,  Yingfei Yang

IPC: A61K31/472 ,  C07D217/06 ,  A61K31/18 ,  A61K31/47 ,  C07D417/04 ,  A61K31/4725 ,  A61K45/06

CPC classification number: C07D217/06 ,  A61K31/18 ,  A61K31/47 ,  A61K31/472 ,  A61K31/4725 ,  A61K45/06 ,  C07D417/04

Abstract: The present invention relates to compounds which are inhibitors of microsomal triglyceride transfer protein and/or apolipoprotein B (Apo B) secretion. These compounds can be useful for the prevention and treatment of various diseases, particularly atherosclerosis and its clinical sequelae, for lowering serum lipids, and related ailments. The invention further relates to pharmaceutical compositions comprising the compounds and to methods of treating diseases, such as hypertriglyceridemia, hyperchylomicronemia, atherosclerosis, obesity, and related conditions using the compounds. A method for decreasing apolipoprotein B (apo B) secretion is also provided.

公开(公告)号：US20090325262A1

公开(公告)日：2009-12-31

申请号：US12081650

申请日：2008-04-18

Applicant: Christian Hodneland ,  Stewart Campbell ,  David Duffy ,  Melina Agosto ,  Evelyn Wang

Inventor： Christian Hodneland ,  Stewart Campbell ,  David Duffy ,  Melina Agosto ,  Evelyn Wang

IPC: C12N11/14 ,  C07K17/14

CPC classification number: B82Y30/00 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00432 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00574 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00677 ,  B01J2219/00686 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00727 ,  B82Y40/00 ,  C07B2200/11 ,  C07K1/1077 ,  C12N9/18 ,  C12N11/14 ,  C40B40/00 ,  G01N33/54353

Abstract: The present invention provides an article for immobilizing functional organic biomolecules through a covalent bond to a thiolate monolayer on a coinage metal surface. Also provided are methods for making the article and method for the immobilization of functional organic biomolecules on the article. The thiolate monolayer contains two moieties, one having an inert group that is resistant to reacting with biomolecules and one having a covalent bond forming group that reacts with the functional organic biomolecule to covalently immobilize it on the monolayer.

Abstract translation: 本发明提供了通过共价键将功能性有机生物分子固定在造币金属表面上的硫醇盐单层的制品。 还提供了制备用于将功能性有机生物分子固定在制品上的制品和方法的方法。 硫醇盐单层包含两个部分，一个具有与生物分子反应的惰性基团，一个具有共价键形成基团的官能团与功能性有机生物分子反应以将其共价固定在单层上。

公开(公告)号：US07476523B2

公开(公告)日：2009-01-13

申请号：US09929736

申请日：2001-08-14

Applicant: Olivier Schueller ,  Enoch Kim ,  George Whitesides

Inventor： Olivier Schueller ,  Enoch Kim ,  George Whitesides

IPC: C12N11/00 ,  C12N11/14 ,  C12N11/02 ,  C12N11/08 ,  B41F31/00 ,  B41K1/38 ,  C07K17/00 ,  C07K17/02 ,  C07K17/08 ,  C07K17/14

CPC classification number: B82Y10/00 ,  B05D1/283 ,  B82Y30/00 ,  B82Y40/00 ,  G03F7/0002 ,  Y10S530/81 ,  Y10S530/811 ,  Y10S530/812 ,  Y10S530/815

Abstract: A deformable stamp for patterning a surface. The stamp can be placed in contact with an entire 3-dimensional object, such as a rod, in a single step. The stamp can also be used to pattern the inside of a tube or rolled over a surface to form a continuous pattern. The stamp may also be used for fluidic patterning by flowing material through channels defined by raised and recessed portions in the surface of the stamp as it contacts the substrate. The stamp may be used to deposit self-assembled monolayers, biological materials, metals, polymers, ceramics, or a variety of other materials. The patterned substrates may be used in a variety of engineering and medical applications.

Abstract translation: 用于图案化表面的可变形印模。 邮票可以在一个步骤中与整个三维物体（例如杆）接触。 印模也可以用于对管的内部进行图案化或者在表面上滚动以形成连续图案。 当印模接触基板时，印模也可以通过流动材料流过印模表面中由凸起和凹陷部分限定的通道而用于流体图案化。 邮票可用于沉积自组装单层，生物材料，金属，聚合物，陶瓷或各种其他材料。 图案化的基底可以用于各种工程和医疗应用。

公开(公告)号：US07338613B2

公开(公告)日：2008-03-04

申请号：US09950383

申请日：2001-09-10

Applicant: Olivier J. A. Schueller ,  Amar Kendale

Inventor： Olivier J. A. Schueller ,  Amar Kendale

IPC: B44C1/22 ,  B41K1/38

CPC classification number: G03F7/0002 ,  B05D1/283 ,  B82Y10/00 ,  B82Y30/00 ,  B82Y40/00 ,  C23C30/00 ,  H05K3/0079 ,  H05K3/12

Abstract: An automated process for microcontact printing is provided, comprising the steps of providing a substrate and a stamp; automatically aligning the substrate and stamp so that the stamp is aligned relative to the substrate to impart a pattern to the substrate at a desired location and with a desired orientation on the substrate; applying an ink to the stamp, the ink including a molecular species adapted to form a self-assembling monolayer (SAM) on the substrate; contacting the stamp and the substrate; and separating the stamp from the substrate.

Abstract translation: 提供了一种用于微接触印刷的自动化方法，包括提供基板和印模的步骤; 自动对准衬底和印模，使得印模相对于基底对准，以在期望的位置和基板上具有期望取向的基底赋予图案; 将油墨施加到印模上，所述油墨包括适于在所述基材上形成自组装单层（SAM）的分子种类; 使印模和基板接触; 并将印模与基板分开。

公开(公告)号：US07326563B2

公开(公告)日：2008-02-05

申请号：US10241445

申请日：2002-09-12

Applicant: Enoch Kim ,  Johanna R. Cruceta

Inventor： Enoch Kim ,  Johanna R. Cruceta

IPC: C12M1/34 ,  C12M3/00 ,  C12M1/22

CPC classification number: G01N33/5047 ,  B01L3/502753 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2400/0478 ,  B01L2400/049 ,  B01L2400/086 ,  G01N33/5029 ,  G01N33/5064 ,  G01N2500/00

Abstract: A device for monitoring leukocyte migration is provided. The device generally includes a plurality of chambers, each chamber including a channel in which leukocyte migration mediators or endothelial cells are disposed therein. The device is fabricated in the footprint of a standard microtiter plate. The invention also provides a method of using the device to monitor leukocyte migration in the presence of physiological shear flow and therefore simulate physiological conditions of a blood vessel in vivo. The invention further provides a method of using the device to high-throughput screen a plurality of test agents.

Abstract translation: 提供了一种用于监测白细胞迁移的装置。 该装置通常包括多个腔室，每个腔室包括其中设置有白细胞迁移介质或内皮细胞的通道。 该器件在标准微量滴定板的占位面积中制造。 本发明还提供了一种使用该装置在存在生理剪切流的情况下监测白细胞迁移并因此模拟体内血管生理条件的方法。 本发明还提供了一种使用该设备来高通量筛选多个测试代理的方法。

公开(公告)号：US07166459B2

公开(公告)日：2007-01-23

申请号：US10097329

申请日：2002-03-15

Applicant: Gregory Kirk ,  Matthew Brown ,  Emanuele Ostuni ,  Enoch Kim ,  Bernardo Aumond ,  Olivier Schueller ,  Paul Sweetnam ,  Brian Benoit

Inventor： Gregory Kirk ,  Matthew Brown ,  Emanuele Ostuni ,  Enoch Kim ,  Bernardo Aumond ,  Olivier Schueller ,  Paul Sweetnam ,  Brian Benoit

IPC: C12M1/34

CPC classification number: B01L3/5085 ,  B01D2325/08 ,  B01J2219/00317 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/5025 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0829 ,  B01L2300/0887 ,  B82Y30/00 ,  C12Q1/025 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1468 ,  G01N33/54366 ,  G01N2015/1497 ,  G06T7/0012 ,  G06T7/70 ,  G06T2207/30072 ,  Y10S435/81

Abstract: The present invention discloses a test device including a support member; a top member mounted to the support member, wherein in the support member and the top member are configured such that they together define a discrete chamber. The discrete chamber includes a first well region including at least one first well; a second well region including at least one second well; and a channel region including at least one channel connecting the first well region and the second well region with one another. The second region is preferably horizontally offset with respect to the first well region in a test orientation of the device.

Abstract translation: 本发明公开了一种包括支撑构件的测试装置， 安装到所述支撑构件的顶部构件，其中在所述支撑构件和所述顶部构件中，所述顶部构件被构造成使得它们在一起限定离散的室。 离散室包括包括至少一个第一井的第一井区; 包括至少一个第二井的第二井区; 以及沟道区域，其包括将第一阱区域和第二阱区域彼此连接的至少一个沟道。 在设备的测试方向上，第二区域优选地相对于第一阱区水平偏移。

公开(公告)号：US06844184B2

公开(公告)日：2005-01-18

申请号：US10206536

申请日：2002-07-29

Applicant: Enoch Kim ,  Gregory L. Kirk ,  Olivier Schueller ,  Emanuele Ostuni

Inventor： Enoch Kim ,  Gregory L. Kirk ,  Olivier Schueller ,  Emanuele Ostuni

IPC: B01L3/00 ,  C12M3/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/543 ,  G06T7/00 ,  C12M1/34

CPC classification number: C12M23/12 ,  B01D2325/08 ,  B01J2219/00317 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/5085 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0829 ,  B01L2300/0887 ,  B82Y30/00 ,  C12M25/06 ,  C12M41/46 ,  C12Q1/025 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1468 ,  G01N33/54366 ,  G01N2015/1497 ,  G06K9/0014 ,  G06T7/0012 ,  G06T7/70 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30072

Abstract: The invention relates to devices, devices for arraying biomolecules, including cells, methods for arraying biomolecules, assays for monitoring cellular movement, and systems for monitoring cellular movement. The devices include a support; a first layer configured to be placed in fluid-tight contact with the support, the first layer having an upper surface and defining a pattern of micro-orifices, each micro-orifice of the pattern of micro-orifices having walls and defining a micro-region on the support when the first layer is placed in fluid-tight contact with the support such that the walls of said each micro-orifice and the micro-region on the support together define a micro-well; and a second layer configured to be placed in fluid-tight contact with the upper surface of the first layer, the second layer defining a pattern of macro-orifices, each macro-orifice of the pattern of macro-orifices having walls and defining a macro-region when the first layer is placed in fluid-tight contact with the support and the second layer is placed in fluid-tight contact with the first layer such that the walls of the macro-orifice and the macro-region together define a macro-well.

Abstract translation: 本发明涉及用于排列生物分子的装置，包括细胞，用于排列生物分子的方法，用于监测细胞运动的测定和用于监测细胞运动的系统。 这些装置包括一个支架; 第一层，其被配置为与所述支撑件流体密封接触，所述第一层具有上表面并限定微孔图案，所述微孔图案的每个微孔具有壁并限定微孔， 当第一层与支撑件流体密封地接触时，支撑件上的区域在支撑件上，使得所述每个微孔的壁和支撑件上的微区域一起限定微孔; 以及第二层，其被配置为与所述第一层的上表面流体密封接触，所述第二层限定宏观孔的图案，所述宏观孔的图案的每个宏观孔口具有壁并且限定宏观 当所述第一层与所述支撑件流体密封接触时，所述第二层与所述第一层流体密封接触，使得所述宏观孔和所述宏观区域的壁一起限定宏观尺寸， 好。

公开(公告)号：US20040261981A1

公开(公告)日：2004-12-30

申请号：US10713619

申请日：2003-11-13

Applicant: Surface Logix, Inc.

Inventor： Christopher H. McCoy ,  John T. Chen ,  David R. Beaulieu

IPC: B32B003/10

CPC classification number: H01L23/3737 ,  H01L21/4871 ,  H01L23/3677 ,  H01L23/3733 ,  H01L2924/0002 ,  Y10T428/24273 ,  H01L2924/00

Abstract: A thermal management material that may be used is thermal interface material is described. An apparatus and methods of the making the thermal management material are also described, which includes a roll-to-roll apparatus for making the thermal management material.

Abstract translation: 可以使用的热管理材料是热界面材料。 还描述了制造热管理材料的装置和方法，其包括用于制造热管理材料的卷对卷装置。

公开(公告)号：US07419823B2

公开(公告)日：2008-09-02

申请号：US10688904

申请日：2003-10-21

Applicant: Gregory L. Kirk ,  Emanuele Ostuni ,  Enoch Kim ,  Olivier Schueller ,  Paul Sweetnam

Inventor： Gregory L. Kirk ,  Emanuele Ostuni ,  Enoch Kim ,  Olivier Schueller ,  Paul Sweetnam

IPC: C12M1/36 ,  C12M1/38 ,  C12M3/00 ,  C12M1/34 ,  C12M1/22

CPC classification number: G01N33/5029 ,  G01N33/5047 ,  G01N33/5064

Abstract: A device for monitoring leukocyte migration is provided. The invention also provides a method of using the device to monitor leukocyte migration in the presence of physiological shear flow and therefore simulate physiological conditions of a blood vessel in vivo. The invention further provides a method of using the device to high-throughput screen a plurality of test agents. The present invention further provides a flexible assay system and numerous assays that can be used to test biological interactions and systems. Laminar flow gradients are employed that mimic gradient situations present in vivo.

Abstract translation: 提供了一种用于监测白细胞迁移的装置。 本发明还提供了一种使用该装置在存在生理剪切流的情况下监测白细胞迁移并因此模拟体内血管生理条件的方法。 本发明还提供了一种使用该设备来高通量筛选多个测试代理的方法。 本发明进一步提供灵活的测定系统和可用于测试生物相互作用和系统的许多测定。 使用模拟体内存在的梯度情况的层流流动梯度。

公开(公告)号：US07371563B2

公开(公告)日：2008-05-13

申请号：US10206074

申请日：2002-07-29

Applicant: David Duffy ,  Gregory L. Kirk ,  Stewart Campbell ,  Olivier Schueller ,  Melina Amber Agosto ,  Enoch Kim

Inventor： David Duffy ,  Gregory L. Kirk ,  Stewart Campbell ,  Olivier Schueller ,  Melina Amber Agosto ,  Enoch Kim

IPC: C12M1/34 ,  C12M3/00 ,  C12M1/22 ,  B05C17/06

CPC classification number: G01N33/54366 ,  B01D2325/08 ,  B01J2219/00274 ,  B01J2219/00317 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/5085 ,  B01L2200/021 ,  B01L2200/025 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0829 ,  B01L2300/0848 ,  B01L2300/0887 ,  B01L2300/123 ,  B82Y5/00 ,  B82Y30/00 ,  C12M23/12 ,  C12Q1/025 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1468 ,  G01N2015/1497 ,  G06T7/0012 ,  G06T7/70 ,  G06T2207/30072 ,  Y10T428/24281 ,  Y10T428/24289 ,  Y10T428/24306

Abstract: Devices and methods for performing assays on materials, particularly biological materials, are provided. The devices and methods make use of self-sealing members, which can be applied to a flat surface to form wells to facilitate immobilization of materials on the flat surface, then removed to yield a flat surface that facilitates the performance of processes on and/or detection of the immobilized material.

Abstract translation: 提供了用于对材料，特别是生物材料进行测定的装置和方法。 这些装置和方法利用自密封构件，其可以应用于平坦表面以形成井，以便于材料在平坦表面上的固定，然后移除以产生平坦表面，其有助于在和/或 检测固定化材料。