公开(公告)号：US20190193050A1

公开(公告)日：2019-06-27

申请号：US16301303

申请日：2017-07-10

Applicant: HITACHI CHEMICAL COMPANY, LTD.

Inventor： Masashi MARUYAMA ,  Nanae YAMASHITA ,  Yuzuru SHIMAZAKI ,  Hiroshi YOSHIDA ,  Keisuke SHIBUYA

IPC: B01J20/26 ,  B01D15/38 ,  B01J20/285 ,  B01D15/02 ,  C12N9/76 ,  C07K16/00 ,  C07K14/76 ,  C07K1/22

CPC classification number: B01J20/26 ,  B01D15/00 ,  B01D15/02 ,  B01D15/08 ,  B01D15/3809 ,  B01D15/3814 ,  B01D15/3823 ,  B01J20/28 ,  B01J20/285 ,  C07K1/22 ,  C07K14/76 ,  C07K16/00 ,  C12M1/26 ,  C12N9/6427 ,  G01N30/88

Abstract: An object of the present invention is to provide an adsorbent material having high dispersibility and reversibility. The adsorbent material has a polymer material having a plurality of functional groups ionizable in water and exhibiting no lower limit critical solution temperature, an adsorption site capable of interacting with a target substance, and a carrier.

公开(公告)号：US4775483A

公开(公告)日：1988-10-04

申请号：US908692

申请日：1986-09-18

Applicant: Sailen S. Mookerjea ,  Arun Nagpurkar ,  Uday Saxena

Inventor： Sailen S. Mookerjea ,  Arun Nagpurkar ,  Uday Saxena

IPC: B01D15/00 ,  B01D15/08 ,  B01J20/32

CPC classification number: B01D15/3809 ,  B01J20/28004 ,  B01J20/3092 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3246 ,  B01J20/3248 ,  B01J20/3251 ,  B01J20/3257 ,  B01J20/3259 ,  B01J20/3261 ,  B01J20/3274 ,  B01J20/3425 ,  B01J20/3475 ,  B01D15/3814 ,  B01D15/426 ,  B01J2220/58

Abstract: Cholesterol in liquids such as blood plasma can be reduced by passing such liquids over an insoluble matrix such as agarose or treated glass beads to which is attached a C-reactive protein capable of binding cholesterol. An eluent such as sodium chloride or phosphorylcholine can be used to remove bound cholesterol and regenerate the insoluble matrix-C-reactive protein combination for re-use.

Abstract translation: 通过将这样的液体通过不溶性基质例如琼脂糖或处理过的玻璃珠，可以降低液体如血浆中的胆固醇，其中附着有能够结合胆固醇的C-反应蛋白。 洗脱液如氯化钠或磷酸胆碱可用于去除结合的胆固醇并再生不溶性基质-C反应蛋白组合以供重复使用。

公开(公告)号：US09909113B2

公开(公告)日：2018-03-06

申请号：US14732662

申请日：2015-06-05

Applicant: RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK

Inventor： David H. Calhoun ,  Abass Abdullahi

IPC: C12N9/40 ,  B01D15/38

CPC classification number: C12N9/2465 ,  B01D15/3814 ,  C12Y302/01022

Abstract: In one embodiment, the invention provides a method of purifying recombinant alpha-galactosidase A. The method includes obtaining a lysate from cells recombinantly expressing alpha-galactosidase A grown in a cell culture medium having non-precipitating phosphate; contacting said lysate with a first chromatography media that binds α-D-mannopyranosyl or α-D-glucopyranosyl; eluting alpha-galactosidase A from the first chromatography media to generate a first eluate having alpha-galactosidase A, wherein said eluting includes at least one elution pause between 4 and 16 hours; contacting the first eluate with a second chromatography media that binds galactose binding proteins; and eluting alpha-galactosidase A from said second chromatography media to generate a second eluate containing said recombinant alpha-galactosidase A.

公开(公告)号：US08343350B2

公开(公告)日：2013-01-01

申请号：US10516405

申请日：2003-06-17

Applicant: Wolfgang Demmer ,  Dietmar Nussbaumer ,  Hans-Heinrich Hörl

Inventor： Wolfgang Demmer ,  Dietmar Nussbaumer ,  Hans-Heinrich Hörl

IPC: B01D15/00

CPC classification number: C07K14/81 ,  B01D15/00 ,  B01D15/3814 ,  B01D61/00 ,  B01D67/0093 ,  B01D69/14 ,  B01J20/28033 ,  B01J20/2808 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3255 ,  B01J20/3274 ,  C07K14/8107

Abstract: The invention comprises a membrane and device and method for removing proteases from fluids, particularly from biological fluids and pharmaceutical solutions, which uses a microporous membrane body, whereby inhibitors that selectively bind proteases are coupled to the membrane body by chemically activated groups.

Abstract translation: 本发明包括用于从流体，特别是从生物流体和药物溶液中去除蛋白酶的膜和装置和方法，其使用微孔膜体，由此通过化学活化的基团将选择性结合蛋白酶的抑制剂偶联到膜体上。

公开(公告)号：US20050245431A1

公开(公告)日：2005-11-03

申请号：US10516405

申请日：2003-06-17

Applicant: Wolfgang Demmer ,  Dietmar Nussbaumer ,  Hans-Heinrich Horl

Inventor： Wolfgang Demmer ,  Dietmar Nussbaumer ,  Hans-Heinrich Horl

IPC: B01D15/00 ,  B01D61/00 ,  B01D67/00 ,  B01D69/14 ,  B01J20/32 ,  C07K14/81 ,  A61K38/17 ,  C12N9/99

CPC classification number: C07K14/81 ,  B01D15/00 ,  B01D15/3814 ,  B01D61/00 ,  B01D67/0093 ,  B01D69/14 ,  B01J20/28033 ,  B01J20/2808 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3255 ,  B01J20/3274 ,  C07K14/8107

Abstract: The invention comprises a membrane and device and method for removing proteases from fluids, particularly from biological fluids and pharmaceutical solutions, which uses a microporous membrane body, whereby inhibitors that selectively bind proteases are coupled to the membrane body by chemically activated groups.

Abstract translation: 本发明包括用于从流体，特别是从生物流体和药物溶液中去除蛋白酶的膜和装置和方法，其使用微孔膜体，由此通过化学活化的基团将选择性结合蛋白酶的抑制剂偶联到膜体上。

公开(公告)号：US20050118686A1

公开(公告)日：2005-06-02

申请号：US10790069

申请日：2004-03-02

Applicant: Chung-Liang Lin ,  Tung-Li Huang ,  Jeen-Kuan Chen ,  Chi-Sheng Wu

Inventor： Chung-Liang Lin ,  Tung-Li Huang ,  Jeen-Kuan Chen ,  Chi-Sheng Wu

IPC: C12P19/28 ,  B01D15/36 ,  B01D15/38 ,  C12P19/26 ,  G01N30/84 ,  C12P19/44

CPC classification number: G01N30/84 ,  B01D15/362 ,  B01D15/3814 ,  C12P19/26 ,  G01N30/461 ,  G01N2030/8429

Abstract: A purification process for manufacturing a high pure acarbose relates to a process for preparing high pure acarbose from acarbose-containing fermentation broth. The acarbose was purified through steps of alcohol precipitation, a strongly acidic cation exchanger chromatography and an immobilized enzyme affinity chromatography. Acarbose is generally applied in treating diabetes.

Abstract translation: 用于制造高纯度阿卡波糖的纯化方法涉及从含阿卡波糖的发酵液制备高纯度阿卡波糖的方法。 阿卡波糖通过醇沉淀，强酸性阳离子交换层析和固定化酶亲和层析的步骤纯化。 阿卡波糖通常用于治疗糖尿病。

公开(公告)号：US20180319838A1

公开(公告)日：2018-11-08

申请号：US15773739

申请日：2016-11-04

Applicant: Biogen MA Inc.

Inventor： Carl CO ,  John E. COUGHLIN

IPC: C07K1/22 ,  C12N11/02 ,  B01J20/286 ,  B01J20/32 ,  B01D15/38 ,  B01D15/20

CPC classification number: C07K1/22 ,  B01D15/20 ,  B01D15/3814 ,  B01D15/3823 ,  B01J20/286 ,  B01J20/3217 ,  C12N11/02 ,  C12Y205/01018

Abstract: The present disclosure relates, in some aspects, to protein-ligand localized conjugation technology with respect to immobilized functional proteins for affinity enrichment and/or modified proteins for therapeutic applications.

公开(公告)号：US09650413B2

公开(公告)日：2017-05-16

申请号：US14627559

申请日：2015-02-20

Applicant: Genzyme Corporation

Inventor： Konstantin Konstantinov ,  Rahul Godawat ,  Veena Warikoo ,  Sujit Jain

IPC: C07K1/22 ,  C07K16/00 ,  C12N9/00 ,  C12P21/00

CPC classification number: C07K1/16 ,  B01D15/1807 ,  B01D15/1864 ,  B01D15/1871 ,  B01D15/327 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01D15/3814 ,  B01D15/422 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/00 ,  C07K2317/14 ,  C12M23/52 ,  C12M47/10 ,  C12M47/12 ,  C12N9/00 ,  C12N9/2402 ,  C12N9/2465 ,  C12P21/00 ,  C12P21/005 ,  C12Y302/01022 ,  C12Y302/01045

Abstract: Provided herein are integrated continuous biomanufacturing processes for producing a therapeutic protein drug substance. Also provided are systems that are capable of continuously producing a therapeutic protein drug substance.

公开(公告)号：US5294341A

公开(公告)日：1994-03-15

申请号：US974178

申请日：1992-11-10

Applicant: Chikafusa Fukazawa

Inventor： Chikafusa Fukazawa

IPC: C12N9/00 ,  B01D15/08 ,  C12N9/26 ,  C12R1/07 ,  C12R1/145

CPC classification number: C12N9/2425 ,  B01D15/3814

Abstract: Disclosed is a method of separating .beta.-amylase from a solution containing .beta.-amylase, in which the solution is treated with an .alpha.-cyclodextrin fixed water-insoluble high polymer compound in the presence of ammonium sulfate to form an adsorbed composite of the high polymer compound and .beta.-amylase and soluble impurities not adsorbed to the high polymer compound are separated and removed. By the method, a high-purity .beta.-amylase, which is highly useful in the starch saccharification industry, may specifically and efficiently be isolated from a .beta.-amylase-containing mixture solution.

Abstract translation: 公开了一种从含有β-淀粉酶的溶液中分离β-淀粉酶的方法，其中溶液在硫酸铵存在下用α-环糊精固定的水不溶性高分子化合物处理以形成高聚物的吸附复合物 分离和除去未吸附到高分子化合物上的化合物和β-淀粉酶和可溶性杂质。 通过该方法，在淀粉糖化工业中非常有用的高纯度β-淀粉酶可以从含有β-淀粉酶的混合物溶液中有效地分离。

公开(公告)号：US4217415A

公开(公告)日：1980-08-12

申请号：US886504

申请日：1978-03-14

Applicant: Raymond C. Barabino ,  Melvin H. Keyes

Inventor： Raymond C. Barabino ,  Melvin H. Keyes

IPC: C12N11/14 ,  B01D15/08 ,  C12Q1/34 ,  C12Q1/40 ,  C07G7/02 ,  C12D13/04 ,  G01N31/08 ,  G01N31/14

CPC classification number: C12Q1/40 ,  B01D15/3814

Abstract: A carbohydrate substrate such as starch for a carbohydrate hydrolyzing enzyme is immobilized on a solid inorganic porous support to form a stable substrate-support composite useful in affinity chromatography and in methods where a precise amount of substrate is needed to perform an enzyme-substrate reaction to quantify the enzyme. The substrate may be activated with an agent such as cyanogen bromide or imidazole prior to deposition on the support so that it may be effectively modified while on the support. After deposition, the substrate is modified by reaction with an epoxyhalogen, aliphatic dihalide or aliphatic diamine to aid in holding it on the support. In an alternative embodiment, the carbohydrate, prior to deposition and modification on the support, is hydrolyzed with an enzyme, preferably dextranase.

Abstract translation: 将碳水化合物底物如用于碳水化合物水解酶的淀粉固定在固体无机多孔载体上以形成可用于亲和层析的稳定的底物载体复合物，以及需要精确量的底物以进行酶 - 底物反应 量化酶。 在沉积到载体上之前，可以用诸如溴化氰或咪唑的试剂来活化底物，使得其可以在载体上被有效地改性。 沉积后，通过与环氧卤素，脂族二卤化物或脂族二胺的反应来改性底物以帮助将其保持在载体上。 在替代实施方案中，在载体上沉积和修饰之前，碳水化合物用酶优选葡聚糖酶水解。