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    NOVEL DIGLYCOSIDASE AND GENE ENCODING THE SAME
    2.
    发明申请
    NOVEL DIGLYCOSIDASE AND GENE ENCODING THE SAME 有权
    新型大分子酶和基因编码它们

    公开(公告)号：US20100221810A1

    公开(公告)日：2010-09-02

    申请号：US11997005

    申请日：2006-07-27

    申请人： Kazutaka Tsuruhami ,  Shigeharu Mori ,  Yoshinao Koide

    发明人： Kazutaka Tsuruhami ,  Shigeharu Mori ,  Yoshinao Koide

    IPC分类号： C12N9/24 ,  C07K14/385 ,  C12N15/31 ,  C12N15/63 ,  C12N1/15

    CPC分类号： C12R1/80 ,  C12N9/2402 ,  C12P19/12

    摘要： A novel diglycosidase produced by a microorganism belonging to the genus Penicillium, having the following physicochemical properties:(1) action and substrate specificity: it acts on a disaccharide glycoside, releasing the disaccharide sugar and the aglycone thereof; (2) optimum pH: around 4.5; (3) pH stability: it is stable at pH 4.0 to 8.0 under the processing condition of 37° C. for 30 minutes, and retains its 80% or more of the activity even after processing at pH 4.0 or lower; (4) optimum temperature: around 60° C. in a sodium acetate-acetic acid buffer solution (pH 5.5); (5) thermal stability: it is stable at 50° C. or lower in a sodium acetate-acetic acid buffer solution (pH 5.5) and retains 45% of the activity even after processing at 60° C. for 40 minutes; (6) molecular weight: 40,000±5,000 Da based on SDS-PAGE measurement; and (7) isoelectric point (pI): about 4.3.

    摘要翻译： 一种由属于青霉属的微生物产生的新型二糖苷酶，具有以下物理化学性质：（1）作用和底物特异性：其作用于二糖苷，释放二糖和其糖苷配基; （2）最适pH：约4.5; （3）pH稳定性：在37℃的加工条件下在pH4.0〜8.0下稳定30分钟，即使在pH4.0以下处理后仍保持80％以上的活性; （4）最适温度：约60℃在乙酸钠 - 乙酸缓冲溶液（pH5.5）中; （5）热稳定性：在乙酸钠 - 乙酸缓冲溶液（pH5.5）中在50℃以下稳定，即使在60℃下处理40分钟后也保持45％的活性; （6）基于SDS-PAGE测定的分子量：40,000±5,000Da; 和（7）等电点（pI）：约4.3。