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    ASSAYS INVOLVING COLLOIDS AND NON-COLLOIDAL STRUCTURES
    94.
    发明申请
    ASSAYS INVOLVING COLLOIDS AND NON-COLLOIDAL STRUCTURES 审中-公开
    涉及胶体和非胶结构的测定

    公开(公告)号：WO0043783A3

    公开(公告)日：2000-11-02

    申请号：PCT/US0001504

    申请日：2000-01-21

    Applicant: MINERVA BIOTECHNOLOGIES CORP ,  BAMDAD CYNTHIA CAROL ,  BAMDAD R SHOSHANA

    Inventor： BAMDAD CYNTHIA CAROL ,  BAMDAD R SHOSHANA

    IPC: G01N33/566 ,  C12Q1/02 ,  G01N21/78 ,  G01N27/12 ,  G01N27/327 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/545 ,  G01N33/548 ,  G01N33/553 ,  G01N37/00

    CPC classification number: B82Y30/00 ,  B82Y5/00 ,  B82Y15/00 ,  G01N33/5438 ,  G01N2610/00 ,  G01N2800/52

    Abstract: The invention provides novel techniques for derivatizing colloids with self-assembled monolayers. This provides the capability of a wide variety of assays including chemical or biochemical agent/agent interaction studies. Bio-derivatized colloids, with or without signaling entities, are used to probe interactions with species on non-colloidal structures. The invention provides techniques for immobilizing colloidal particles on a wide variety of non-colloidal structures. Included is the ability to decorate a variety of non-colloidal structures including beads, with colloids as a detectable assay. This allows, in many cases, assays detectable via the unaided human eye, as well as assays detectable via automated determination of a change of interaction of electromagnetic radiation with the colloids, e.g., absorption, light-scattering, and the like.

    Abstract translation: 本发明提供用自组装单层衍生化胶体的新技术。 这提供了各种测定的能力，包括化学或生物化学试剂/药物相互作用研究。 具有或不具有信号传导实体的生物衍生胶体用于探测与非胶体结构物质的相互作用。 本发明提供了将胶体颗粒固定在各种非胶体结构上的技术。 包括装饰各种非胶体结构（包括珠粒）的能力，胶体作为可检测的测定法。 这在许多情况下允许通过无辅助人眼可检测的测定，以及通过自动测定电磁辐射与胶体的相互作用变化（例如吸收，光散射等）可检测的测定。

    INTERACTION OF COLLOID-IMMOBILIZED SPECIES WITH SPECIES ON NON-COLLOIDAL STRUCTURES
    95.
    发明申请
    INTERACTION OF COLLOID-IMMOBILIZED SPECIES WITH SPECIES ON NON-COLLOIDAL STRUCTURES 审中-公开
    胶体固定物种与非晶体结构物种的相互作用

    公开(公告)号：WO00043783A2

    公开(公告)日：2000-07-27

    申请号：PCT/US2000/001504

    申请日：2000-01-21

    IPC: G01N33/566 ,  C12Q1/02 ,  G01N21/78 ,  G01N27/12 ,  G01N27/327 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/545 ,  G01N33/548 ,  G01N33/553 ,  G01N37/00

    CPC classification number: B82Y30/00 ,  B82Y5/00 ,  B82Y15/00 ,  G01N33/5438 ,  G01N2610/00 ,  G01N2800/52

    Abstract: The invention provides novel techniques for derivatizing colloids with self-assembled monolayers. This provides the capability of a wide variety of assays including chemical or biochemical agent/agent interaction studies. Bio-derivatized colloids, with or without signaling entities, are used to probe interactions with species on non-colloidal structures. The invention provides techniques for immobilizing colloidal particles on a wide variety of non-colloidal structures. Included is the ability to decorate a variety of non-colloidal structures including beads, with colloids as a detectable assay. This allows, in many cases, assays detectable via the unaided human eye, as well as assays detectable via automated determination of a change of interaction of electromagnetic radiation with the colloids, e.g., absorption, light-scattering, and the like.

    Abstract translation: 本发明提供用自组装单层衍生化胶体的新技术。 这提供了各种测定的能力，包括化学或生物化学试剂/药物相互作用研究。 具有或不具有信号传导实体的生物衍生胶体用于探测与非胶体结构物质的相互作用。 本发明提供了将胶体颗粒固定在各种非胶体结构上的技术。 包括装饰各种非胶体结构（包括珠粒）的能力，胶体作为可检测的测定法。 这在许多情况下允许通过无辅助人眼可检测的测定，以及通过自动测定电磁辐射与胶体的相互作用变化（例如吸收，光散射等）可检测的测定。

    METHOD FOR ISOLATING, IN PARTICULAR FOR DETECTING OR QUANTIFYING AN ANALYTE IN A MEDIUM
    96.
    发明申请
    METHOD FOR ISOLATING, IN PARTICULAR FOR DETECTING OR QUANTIFYING AN ANALYTE IN A MEDIUM 审中-公开
    用于分离，特别是用于检测或定量分析仪在中等的方法

    公开(公告)号：WO98034116A1

    公开(公告)日：1998-08-06

    申请号：PCT/FR1998/000182

    申请日：1998-01-30

    IPC: G01N33/553 ,  G01N33/543 ,  G01N33/548 ,  G01N33/76

    CPC classification number: G01N33/54326 ,  B01J2219/00648 ,  B01J2219/00659 ,  G01N33/54306 ,  Y10S436/801 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/824 ,  Y10T436/24

    Abstract: The invention concerns a method for isolating at least an analyte (4) in a medium, in which it is distributed, consisting in: (a) providing at least one reagent (3) comprising a free support, in discrete form, exhibiting at least a receptor of the analyte; (b) providing another capture support, the accessible surface of which comprises at least an effective or active zone, of limited surface, exhibiting at least another receptor (10) of an entity to be captured, viz. of said analyte or said receptor; (c) contacting the reagent (3) with a liquid sample obtained from the medium containing the analyte, for forming an intermediate medium comprising, also in discrete form and distributed in said medium, a complex between said support, the receptor and the analyte; (d) contacting the accessible surface of said other support with at least a capture reactant, viz. the reagent that has not reacted during step (c) or the complex, for binding said capture reactant to said accessible surface, and obtaining a concentrated deposit in said effective zone, fixed on said other support by the bond of said other receptor with said receptor or analyte; (e) concentrating the deposit of said capture reactant in said effective zone, by increasing the amount of said reactant per surface unit in said effective zone.

    Abstract translation: 本发明涉及一种用于分离其中分布的介质中的至少一种分析物（4）的方法，其包括：（a）提供至少一种试剂（3），其包含离散形式的至少显示出离散形式的游离载体 分析物的受体; （b）提供另一个捕获载体，其可接近的表面包含至少一个有限表面的有效或活性区域，其表现出待被捕获的实体的至少另一个受体（10），即， 的所述分析物或所述受体; （c）使试剂（3）与从含有分析物的培养基获得的液体样品接触，以形成中间培养基，其也以离散形式并分布在所述培养基中，所述载体，受体和分析物之间的复合物; （d）使所述其它载体的可接近表面与至少一种捕获反应物接触，即， 步骤（c）中没有反应的试剂或络合物，用于将所述捕获反应物结合到所述可接近表面，并且通过所述另一受体与所述受体的键固定在所述有效区中的浓缩沉积物 或分析物; （e）通过增加所述有效区域中每个表面单元的反应物的量来浓缩所述捕获反应物在所述有效区域中的沉积物。

    앱타머를 이용한 인플루엔자 바이러스 검출방법
    100.
    发明申请
    앱타머를 이용한 인플루엔자 바이러스 검출방법 审中-公开

    公开(公告)号：WO2021215875A1

    公开(公告)日：2021-10-28

    申请号：PCT/KR2021/005175

    申请日：2021-04-23

    Applicant: ㈜에스비바이오사이언스

    Inventor： 박진주 ,  염규호 ,  강주영 ,  김민곤

    IPC: G01N33/569 ,  G01N33/548 ,  G01N33/68 ,  C12N15/115 ,  C12Q1/6806

    Abstract: 본 발명은 인플루엔자 바이러스 유래 뉴클레오캡시드 단백질(NP)에 특이적으로 결합하는 앱타머 및 상기 앱타머를 검출하기 위한 복합체를 포함하는 인플루엔자 바이러스 검출용 조성물, 상기 조성물을 포함하는 인플루엔자 바이러스 검출용 키트, 상기 조성물 또는 키트를 사용하여 인플루엔자 바이러스의 감염여부 확인에 필요한 정보를 제공하는 방법 및 인플루엔자 감염여부 확인을 위한 상기 조성물의 용도에 관한 것이다. 본 발명에서 제공하는 앱타머를 이용한 인플루엔자 바이러스 검출방법을 이용하면, 환자가 인플루엔자 바이러스에 감염되었는지의 여부를 높은 민감도와 특이도로서 검출할 수 있으므로, 인플루엔자 바이러스의 보다 효과적인 치료에 널리 활용될 수 있을 것이다.

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