摘要:
A microfiltration apparatus and method for separating cells, such as circulating tumor cells, from a sample using a microfiltration device having a top porous membrane and a bottom porous membrane. The porous membranes are formed from parylene and assembled using microfabrication techniques. The porous membranes are arranged so that the pores in the top membrane are offset from the pores in the bottom membrane.
摘要:
A microfiltration apparatus and method for separating cells, such as circulating tumor cells, from a sample using a microfiltration device having a top porous membrane and a bottom porous membrane. The porous membranes are formed from parylene and assembled using microfabrication techniques. The porous membranes are arranged so that the pores in the top membrane are offset from the pores in the bottom membrane.
摘要:
The present invention provides methods for designing a filtration systems for capturing viable tumor cells, such as circulating tumor cells at high efficiency and high viability. The methods involve development of a set of “key engineering design parameters” that are crucial to achieve high tumor cell viability. These important design parameters include the filter geometry design, fluid delivery method, drive pressure and filtration time.
摘要:
A diagnosable magnetic switch assembly and method for determining a seat occupant. A network in the assembly includes a resistor in series with a magnetic switch, and a resistor in parallel with the series combination. Such an arrangement provides distinct assembly resistance values when the magnetic switch is open or closed, and thereby provides a means for diagnosing the operational state of the diagnosable magnetic switch assembly. The diagnosable magnetic switch assembly may be included in a seat occupant indicator system that embeds the seat occupant indicator system and a magnet in a cushion of a seat assembly. When the seat assembly is occupied, the magnet is separated from the seat occupant indicator system by a sufficient distance so the seat occupant indicator system is in an open state. When an occupant sits in the seat assembly, the magnet and the seat occupant indicator system are brought closer together so that the magnetic field operate the seat occupant indicator system to a closed state. A controller measures the assembly resistance values to determine if the seat assembly is empty, occupied, or if an assembly fault state is occurring.
摘要:
Targeted advertisements to social contacts is described. In embodiment(s), social contacts can be correlated based on messaging interactions between mobile communication devices that are associated with the social contacts. Feedback about an advertisement can be received from mobile communication devices that are associated with the social contacts, and the advertisement can then be targeted to one or more of the social contacts based on the feedback about the advertisement.
摘要:
A microfiltration apparatus and method for separating cells, such as circulating tumor cells, from a sample using a microfiltration device having a top porous membrane and a bottom porous membrane. The porous membranes are formed from parylene and assembled using microfabrication techniques. The porous membranes are arranged so that the pores in the top membrane are offset from the pores in the bottom membrane.
摘要:
A method of fabricating a nonvolatile memory cell includes providing a substrate with a trench, with a sidewall where a tunnel oxide layer and a floating gate are successively formed, forming a control gate in the trench, performing a high density plasma deposition process to form an HDP oxide layer on the top surface of control gate.
摘要:
The present invention provides methods for designing a filtration systems for capturing viable tumor cells, such as circulating tumor cells at high efficiency and high viability. The methods involve development of a set of “key engineering design parameters” that are crucial to achieve high tumor cell viability. These important design parameters include the filter geometry design, fluid delivery method, transfilter pressure and total filtration time.
摘要:
Thin parylene C membranes having smooth front sides and ultrathin regions (e.g., 0.01 μm to 5 μm thick) interspersed with thicker regions are disclosed. The back sides of the membranes can be rough compared with the smooth front sides. The membranes can be used in vitro to grow monolayers of cells in a laboratory or in vivo as surgically implantable growth layers, such as to replace the Bruch's membrane in the eye. The thin regions of parylene are semipermeable to allow for proteins in serum to pass through, and the thick regions give mechanical support for handling by a surgeon. The smooth front side allows for monolayer cell growth, and the rough back side helps prevents cells from attaching there.
摘要:
The present invention provides a method for diagnosing cancer, predicting a disease outcome or response to therapy in a patient sample. The method involves isolating a circulating tumor cell (CTC), for example, a viable CTC, from a sample using a parylene microfilter device comprising a membrane filter having or consisting of a parylene substrate, which has an array of holes with a predetermined shape and size; and detecting and quantifying telomerase activity in blood circulating tumor cells. The invention further provides methods of using cells live-captured in various applications.