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    Methods for isolating nucleic acids
    2.
    发明授权
    Methods for isolating nucleic acids 有权
    分离核酸的方法

    公开(公告)号：US07329491B2

    公开(公告)日：2008-02-12

    申请号：US10962916

    申请日：2004-10-12

    申请人： Michael Kirchgesser ,  Frank Bergmann ,  Thomas Walter ,  Kurt Weindel ,  Ralf Zielenski ,  Emad Sarofim

    发明人： Michael Kirchgesser ,  Frank Bergmann ,  Thomas Walter ,  Kurt Weindel ,  Ralf Zielenski ,  Emad Sarofim

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/1006 ,  C12N15/1003 ,  C12N15/1013

    摘要： A method for purification of a nucleic acid comprising the steps of (a) adsorbing on a substrate the nucleic acid from a composition containing (i) an aqueous buffer, (ii) salts in a high concentration, (iii) a water-miscible, non-acidic organic compound, and (iv) the nucleic acid; (b) optionally washing with a washing solution the substrate with the adsorbed nucleic acid; (c) contacting the substrate with the adsorbed nucleic acid with a solution containing salts in a lower concentration compared to the composition of step (a), thereby desorbing the nucleic acid from the substrate; (d) separating the solution with the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid; and optionally (e) precipitating the desorbed nucleic acid from the solution of step (d) and isolating the precipitated nucleic acid, thereby further purifying the nucleic acid.

    摘要翻译： 一种用于纯化核酸的方法，包括以下步骤：（a）从含有（i）水性缓冲液，（ii）高浓度盐，（iii）水混溶性， 非酸性有机化合物，和（iv）核酸; （b）任选地用洗涤溶液洗涤具有吸附的核酸的底物; （c）与步骤（a）的组合物相比，使含有较低浓度盐的溶液与吸附的核酸接触，从而从底物解吸核酸; （d）将解离的核酸与底物分离，从而纯化核酸; 和任选地（e）从步骤（d）的溶液沉淀解吸的核酸并分离沉淀的核酸，由此进一步纯化核酸。

    Methods for isolating nucleic acids
    3.
    发明申请
    Methods for isolating nucleic acids 有权
    分离核酸的方法

    公开(公告)号：US20050079535A1

    公开(公告)日：2005-04-14

    申请号：US10962916

    申请日：2004-10-12

    申请人： Michael Kirchgesser ,  Frank Bergmann ,  Thomas Walter ,  Kurt Weindel ,  Ralf Zielenski ,  Emad Sarofim

    发明人： Michael Kirchgesser ,  Frank Bergmann ,  Thomas Walter ,  Kurt Weindel ,  Ralf Zielenski ,  Emad Sarofim

    IPC分类号： C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12N15/1006 ,  C12N15/1003 ,  C12N15/1013

    摘要： A method for purification of a nucleic acid comprising the steps of (a) adsorbing on a substrate the nucleic acid from a composition containing (i) an aqueous buffer, (ii) salts in a high concentration, (iii) a water-miscible, non-acidic organic compound, and (iv) the nucleic acid; ( b) optionally washing with a washing solution the substrate with the adsorbed nucleic acid; (c) contacting the substrate with the adsorbed nucleic acid with a solution containing salts in a lower concentration compared to the composition of step (a), thereby desorbing the nucleic acid from the substrate; (d) separating the solution with the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid; and optionally (e) precipitating the desorbed nucleic acid from the solution of step (d) and isolating the precipitated nucleic acid, thereby further purifying the nucleic acid.

    摘要翻译： 一种用于纯化核酸的方法，包括以下步骤：（a）从含有（i）水性缓冲液，（ii）高浓度盐的组合物，（iii）水混溶性， 非酸性有机化合物，和（iv）核酸; （b）任选地用洗涤溶液洗涤具有吸附的核酸的底物; （c）与步骤（a）的组合物相比，使含有较低浓度盐的溶液与吸附的核酸接触，从而从底物解吸核酸; （d）将解离的核酸与底物分离，从而纯化核酸; 和任选地（e）从步骤（d）的溶液沉淀解吸的核酸并分离沉淀的核酸，由此进一步纯化核酸。

    White trigger preparations for improving the signal detection in bioluminescent and chemiluminescent reactions
    7.
    发明授权
    White trigger preparations for improving the signal detection in bioluminescent and chemiluminescent reactions 失效
    用于改善生物发光和化学发光反应中的信号检测的白色触发剂制剂

    公开(公告)号：US06197594B1

    公开(公告)日：2001-03-06

    申请号：US08552795

    申请日：1995-11-03

    申请人： Kurt Weindel ,  Hans Hornauer

    发明人： Kurt Weindel ,  Hans Hornauer

    IPC分类号： G01N2176

    CPC分类号： G01N33/582 ,  G01N21/76 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/807

    摘要： The present invention concerns a method for the detection of an analyte in a sample liquid by luminescence measurement according to the principle of a ligand-receptor assay, e.g. an immunoassay or a hybridization assay or a combination thereof, wherein a sample liquid is incubated with at least one receptor which carries a luminescent label and the presence or/and the amount of the analyte to be detected is determined in the sample liquid by measuring the luminescence in a measuring medium containing dispersed components.

    摘要翻译： 本发明涉及通过根据配体 - 受体测定法的原理的发光测量来检测样品液体中的分析物的方法，例如， 免疫测定或杂交测定或其组合，其中将样品液体与携带发光标记的至少一种受体一起孵育，并且通过测量样品液体来测定待检测的分析物的存在或/和量， 含有分散组分的测量介质中的发光。

    Materials and methods relating to binding assays
    8.
    发明授权
    Materials and methods relating to binding assays 失效
    与结合测定相关的材料和方法

    公开(公告)号：US5830762A

    公开(公告)日：1998-11-03

    申请号：US796806

    申请日：1997-02-06

    申请人： Kurt Weindel

    发明人： Kurt Weindel

    IPC分类号： G01N33/543 ,  G01N35/02 ,  G01N33/50 ,  G01N33/53

    CPC分类号： G01N33/543 ,  G01N33/54393 ,  Y10T436/10

    摘要： Methods of optimising binding assays are disclosed using binding agents having binding sites specific for an analyte by varying the density of binding sites on a solid phase, advantageously extending the working range of the assay and/or improving the precision of the assay within a given range. Processes for manufacturing an assay kit following the optimisation method and to methods of carrying out assays for analytes using these assay kits are also provided.

    摘要翻译： 使用具有对分析物特异性结合位点的结合剂，通过改变固相结合位点的密度，有利地扩展测定的工作范围和/或提高测定精确度在给定范围内来公开优化结合测定的方法 。 还提供了优化方法制备测定试剂盒的方法和使用这些测定试剂盒进行分析物测定的方法。