Method for producing RFeB-based magnet
    1.
    发明授权
    Method for producing RFeB-based magnet 有权
    制造基于RFeB的磁体的方法

    公开(公告)号:US09368277B2

    公开(公告)日:2016-06-14

    申请号:US14492444

    申请日:2014-09-22

    申请人: Shinobu Takagi

    发明人: Shinobu Takagi

    摘要: Provided is a method for producing an RFeB-based magnet, the method including: disposing a nozzle so as to be opposed to an attachment surface of a base material that is a sintered magnet or hot-plastic worked magnet composed of an RFeB-based magnet containing a light rare earth element RL that is at least one element selected from the group consisting of Nd and Pr, Fe, and B; ejecting a mixture, from the nozzle, obtained by mixing an organic solvent and an RH-containing powder containing a heavy rare earth element RH that is at least one element selected from the group consisting of Dy, Tb and Ho so as to attach the mixture to the attachment surface; and heating the base material together with the mixture.

    摘要翻译: 本发明提供一种RFeB系磁体的制造方法,其特征在于,包括:将喷嘴配置成与由烧结磁体构成的基材的配合面或由RFeB系磁铁构成的热塑性加工磁铁 含有选自Nd和Pr,Fe和B中的至少一种元素的轻稀土元素RL; 喷射通过混合有机溶剂和含有选自Dy,Tb和Ho中的至少一种元素的重稀土元素RH的含RH粉末得到的混合物,以便将混合物 到附件表面; 并与混合物一起加热基材。

    METHOD FOR PRODUCING RFeB-BASED MAGNET
    2.
    发明申请
    METHOD FOR PRODUCING RFeB-BASED MAGNET 有权
    用于生产基于RFeB的磁体的方法

    公开(公告)号:US20150086710A1

    公开(公告)日:2015-03-26

    申请号:US14492444

    申请日:2014-09-22

    申请人: Shinobu TAKAGI

    发明人: Shinobu TAKAGI

    摘要: Provided is a method for producing an RFeB-based magnet, the method including: disposing a nozzle so as to be opposed to an attachment surface of a base material that is a sintered magnet or hot-plastic worked magnet composed of an RFeB-based magnet containing a light rare earth element RL that is at least one element selected from the group consisting of Nd and Pr, Fe, and B; ejecting a mixture, from the nozzle, obtained by mixing an organic solvent and an RH-containing powder containing a heavy rare earth element RH that is at least one element selected from the group consisting of Dy, Tb and Ho so as to attach the mixture to the attachment surface; and heating the base material together with the mixture.

    摘要翻译: 本发明提供一种RFeB系磁体的制造方法,其特征在于,包括:将喷嘴配置成与由烧结磁体构成的基材的配合面或由RFeB系磁铁构成的热塑性加工磁铁 含有选自Nd和Pr,Fe和B中的至少一种元素的轻稀土元素RL; 喷射通过混合有机溶剂和含有选自Dy,Tb和Ho中的至少一种元素的重稀土元素RH的含RH粉末得到的混合物,以便将混合物 到附件表面; 并与混合物一起加热基材。

    Lipoxygenase
    4.
    发明授权
    Lipoxygenase 失效
    脂氧合酶

    公开(公告)号:US07456001B2

    公开(公告)日:2008-11-25

    申请号:US10362776

    申请日:2001-09-05

    CPC分类号: C12N9/0069

    摘要: The invention provides sequence information of a microbial protein having lipoxy-genase activity and a method of producing the protein by recombinant DNA technology. More specifically, the inventors have isolated a gene encoding a lipoxygenase from Gaeu-mannomyces graminis, cloned it into an E. coli strain and sequenced it. A comparison shows less than 25% identity to known lipoxygenase sequences, the closest being human 15S li-poxygenase. The inventors have expressed the lipoxygenase recombinantly and found that the recombinant lipoxygenase is glycosylated.

    摘要翻译: 本发明提供具有脂氧合酶活性的微生物蛋白质的序列信息和通过重组DNA技术生产该蛋白质的方法。 更具体地说,本发明人已经分离出来自禾谷镰刀菌的编码脂氧合酶的基因,将其克隆到大肠杆菌菌株中并对其进行测序。 比较显示与已知的脂氧合酶序列具有小于25%的同一性,最接近的是人15S li-加氧酶。 本发明人重组表达了脂氧合酶,发现重组脂氧合酶被糖基化。

    Endoglucanases
    6.
    发明授权
    Endoglucanases 失效
    内切葡聚糖酶

    公开(公告)号:US08642730B2

    公开(公告)日:2014-02-04

    申请号:US12567302

    申请日:2009-09-25

    IPC分类号: C07K1/00

    摘要: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys(SEQ ID NO: 80) 1   2   3   4   5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    摘要翻译: 本发明涉及基本上由具有纤维素分解活性的酶组成并包含具有以下序列的第一氨基酸序列的酶制剂(SEQ ID NO:79)Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 123456789101112 Trp Xaa 1314 和具有以下序列的第二氨基酸序列Trp Cys Cys Xaa Cys(SEQ ID NO:80)12345其中,在第一序列的3位,氨基酸为Trp,Tyr或Phe; 在第一序列的位置4处,氨基酸是Trp,Tyr或Phe; 在第一序列的位置8,氨基酸是Arg,Lys或His; 在第一序列的第9,10,12和14位,第2个序列的第4位,氨基酸是20个天然存在的氨基酸残基中的任一个,条件是在第一个氨基酸序列 ,(i)当第12位的氨基残基为Ser时,第14位的氨基酸残基不为Ser,(ii)当第12位的氨基残基为Gly时,第14位的氨基酸残基为 Ala在工业应用中表现良好,例如洗衣组合物,用于生物抛光新制造的纺织品,用于提供纤维素织物或衣服的磨损外观以及纸浆处理。 此外,本发明涉及编码这些酶的DNA构建体,提供编码这种酶的基因的方法,生产酶的方法,含有这些酶的酶制剂,以及这些酶用于许多工业应用的用途。

    Corrosion resistant conductive parts
    9.
    发明授权
    Corrosion resistant conductive parts 有权
    耐腐蚀导电部件

    公开(公告)号:US07985487B2

    公开(公告)日:2011-07-26

    申请号:US10981708

    申请日:2004-11-05

    IPC分类号: B32B15/18 C25D3/48 C25D7/00

    摘要: Disclosed is a corrosion resistant conductive part, which is made by forming a thin plated gold layer of thickness 100 nm or less on a stainless steel sheet, and for which high corrosion resistance is guaranteed even with a very thin gold layer. The part is characterized in that the maximum value “v” of atomic concentration of gold given by Auger analysis is at least 98% of the total atomic concentrations of all the elements, which is deemed to be 100%, in the thin gold layer ranging from the surface to the interface of the gold layer and the metal substrate, at which the descending curve of gold crosses the ascending curve of the most predominant component of the substrate metal.

    摘要翻译: 公开了一种耐腐蚀性导电部件,其通过在不锈钢板上形成厚度为100nm以下的薄镀金层而形成,并且即使使用非常薄的金层也能保证高耐腐蚀性。 该部分的特征在于,通过俄歇分析给出的金的原子浓度的最大值“v”是所有元素的总原子浓度的至少98%,其被认为是100%,在薄金层中 从金层和金属基底的表面到界面处,金的下降曲线与基底金属最主要成分的上升曲线交叉。

    Expression of Genes from Gram Negative Bacteria in Fungi
    10.
    发明申请
    Expression of Genes from Gram Negative Bacteria in Fungi 审中-公开
    革兰氏阴性菌在真菌中的表达

    公开(公告)号:US20100261259A1

    公开(公告)日:2010-10-14

    申请号:US12376372

    申请日:2007-08-03

    IPC分类号: C12N1/19 C12N1/15 C07H21/04

    摘要: The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.

    摘要翻译: 本发明提供了在适于工业生产的真菌宿主中重组来自革兰氏阴性细菌的多肽的方法。 在第一方面,本发明涉及在真菌宿主细胞中从革兰氏阴性细菌重组表达多肽的方法,其包括以下步骤:i)提供编码所述多肽的核酸序列,所述核酸序列包含第一 编码真菌信号肽的核酸序列和编码具有至少一个修饰密码子的多肽的第二核酸序列,其中所述修饰不改变由所述密码子编码的氨基酸,并且所述密码子的核酸序列与 存在于所述革兰氏阴性细菌中的野生型核酸序列中相应的密码子; ii)在真菌宿主中表达经修饰的核酸序列。