公开(公告)号：US20220307073A1

公开(公告)日：2022-09-29

申请号：US17835439

申请日：2022-06-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John Brian Hutchison ,  Jeffrey Charles Olson ,  Darren Roy Link

IPC: C12Q1/686

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided.

公开(公告)号：US20220213530A1

公开(公告)日：2022-07-07

申请号：US17574780

申请日：2022-01-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US11351510B2

公开(公告)日：2022-06-07

申请号：US15996253

申请日：2018-06-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Michael Weiner ,  David Marran ,  Jonathan M. Rothberg

IPC: B01F23/41 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6874 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12N15/10 ,  B01J19/00 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  C12Q1/00 ,  G01N15/14 ,  G01N21/05 ,  G01N21/64 ,  G01N35/08 ,  B01F33/3011 ,  G01N27/327 ,  B01L7/00 ,  B01L9/00 ,  G01N35/00 ,  G01N21/03

Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

公开(公告)号：US11077415B2

公开(公告)日：2021-08-03

申请号：US16181256

申请日：2018-11-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yevgeny Yurkovetsky ,  Darren Roy Link ,  Jonathan William Larson

IPC: B01L3/00 ,  B01F13/00 ,  B01F5/04 ,  B01L7/00 ,  B01F3/08 ,  B01F5/00

Abstract: The invention generally relates to methods for forming mixed droplets. In certain embodiments, methods of the invention involve forming a droplet, and contacting the droplet with a fluid stream, wherein a portion of the fluid stream integrates with the droplet to form a mixed droplet.

公开(公告)号：US20190330683A1

公开(公告)日：2019-10-31

申请号：US16460247

申请日：2019-07-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Benjamin J. Miller

IPC: C12Q1/686

Abstract: The invention generally relates to detecting target molecules.

公开(公告)号：US10428369B2

公开(公告)日：2019-10-01

申请号：US15176710

申请日：2016-06-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Miller ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6806 ,  B05B1/08 ,  B05B1/26 ,  B05B7/00 ,  B01F13/00 ,  B01F15/00 ,  B01F3/08 ,  B05B1/02 ,  B01L3/00 ,  B01L7/00

Abstract: The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.

公开(公告)号：US12146134B2

公开(公告)日：2024-11-19

申请号：US15996222

申请日：2018-06-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC: C12N15/10 ,  B01F33/302 ,  B01F33/3031 ,  B01J19/00 ,  B01L3/00 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/04 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6876 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N33/536 ,  G01N33/543 ,  G01N33/68 ,  B01F23/41 ,  B01L7/00 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/6869 ,  G01N21/64 ,  G01N33/542

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

公开(公告)号：US12091710B2

公开(公告)日：2024-09-17

申请号：US17174519

申请日：2021-02-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link

IPC: C12Q1/6848 ,  B01F23/41 ,  B01F33/3011 ,  B01J19/00 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  C12N15/10 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N1/38 ,  G01N15/14 ,  G01N35/00 ,  G01N35/08 ,  B01L7/00 ,  B01L9/00

CPC classification number: C12Q1/6848 ,  B01F23/41 ,  B01F33/3011 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L3/565 ,  B03C5/005 ,  B03C5/026 ,  C12N15/1075 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N1/38 ,  G01N15/147 ,  G01N35/08 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00903 ,  B01J2219/0097 ,  B01J2219/00988 ,  B01L3/502746 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C2201/26 ,  C12Q2525/191 ,  C12Q2535/101 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326

Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

公开(公告)号：US20240150813A1

公开(公告)日：2024-05-09

申请号：US18400468

申请日：2023-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Miller ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6806 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/221 ,  B01L3/00 ,  B05B1/02 ,  B05B1/08 ,  B05B1/26 ,  B05B7/00

CPC classification number: C12Q1/6806 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/2213 ,  B01L3/502761 ,  B01L3/502784 ,  B05B1/02 ,  B05B1/08 ,  B05B1/26 ,  B05B7/0012 ,  B01L7/525 ,  B01L2200/0647 ,  B01L2300/14 ,  B01L2400/0487 ,  Y10T436/2575

Abstract: The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.

公开(公告)号：US20230026713A1

公开(公告)日：2023-01-26

申请号：US17956416

申请日：2022-09-29

Applicant: Bio-Rad Laboratories, Inc. ,  Université de Strasbourg

Inventor： Felix Kleinschmidt ,  Andrew David Griffiths ,  Francois Caron ,  Abdeslam El Harrak ,  Eric Brouzes ,  Darren Roy Link

IPC: G01N1/38 ,  G01N33/53

Abstract: The present invention generally relates to systems and methods to create stable emulsions with low rates of exchange of molecules between microdroplets.