公开(公告)号：US09382521B2

公开(公告)日：2016-07-05

申请号：US13848070

申请日：2013-03-21

Applicant: DSM IP Assets B.V.

Inventor： James G. Metz ,  Jerry M. Kuner ,  James Casey Lippmeier

IPC: C12N9/10 ,  C12N9/00 ,  C12N15/82 ,  C12P7/64

CPC classification number: C12N9/1029 ,  C12N9/93 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  Y02P20/52

Abstract: Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

公开(公告)号：US08859855B2

公开(公告)日：2014-10-14

申请号：US13633770

申请日：2012-10-02

Applicant: DSM IP Assets B.V.

Inventor： Craig A. Weaver ,  Ross Zirkle ,  Daniel H. Doherty ,  James G. Metz

IPC: A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N1/20 ,  C07H21/04 ,  C12P7/64 ,  C12P7/62 ,  C12N9/00 ,  C12N15/52

CPC classification number: C12P7/6427 ,  C12N9/93 ,  C12N15/52 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/62 ,  C12P7/625 ,  C12P7/6472

Abstract: Disclosed are chimeric polyunsaturated fatty acid (PUFA) olyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from Schizochytrium and Thraustochytrium. Disclosed are nucleic acids and proteins encoding such chimeric PUFA PKS proteins and systems, genetically modified organisms comprising such chimeric PUFA PKS proteins and systems, and methods of making and using such chimeric PUFA PKS proteins and systems.

Abstract translation: 公开了嵌合多不饱和脂肪酸（PUFA）合成酮合成酶（PKS）蛋白和嵌合PUFA PKS系统，包括嵌合PUFA PKS蛋白和源自裂殖壶菌和破囊壶菌的系统。 公开了编码这种嵌合PUFA PKS蛋白质和系统的核酸和蛋白质，包含这种嵌合PUFA PKS蛋白质和系统的遗传修饰的生物体，以及制备和使用这种嵌合PUFA PKS蛋白质和系统的方法。

公开(公告)号：US20210395765A1

公开(公告)日：2021-12-23

申请号：US17354558

申请日：2021-06-22

Applicant: Dow AgroSciences LLC ,  DSM IP Assets B.V.

Inventor： Terence A. Walsh ,  Daniel J. Gachotte ,  Cory M. Larsen ,  Scott Bevan ,  P. Ann Owens-Merlo ,  James G. Metz ,  Ross Zirkle

IPC: C12N15/82

Abstract: This disclosure concerns recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US08945875B2

公开(公告)日：2015-02-03

申请号：US13923481

申请日：2013-06-21

Applicant: DSM IP Assets B.V.

Inventor： Paul G. Roessler ,  T. Dave Matthews ,  Tom M. Ramseier ,  James G. Metz

IPC: C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/82 ,  C12N9/88 ,  C12N15/79 ,  C12P21/02

CPC classification number: C12N15/82 ,  C12N9/88 ,  C12N15/79 ,  C12P21/02 ,  C12Y202/01006

Abstract: Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.

Abstract translation: 公开了乙酰乳酸合酶，乙酰乳酸合成酶调节区，α-微管蛋白启动子，破囊壶菌聚酮合成酶（PKS）系统的启动子和脂肪酸去饱和酶启动子的核酸和氨基酸序列，每种来自破囊壶菌属微生物。 还公开了可用于转录破囊索杆菌微生物的重组载体，以及转变破囊壶菌微生物的方法。 本发明的重组核酸分子可用于在破囊壶菌属微生物中表达外源核酸，以及用于在裂囊酵素微生物中的基因的缺失，突变或失活。

公开(公告)号：US11046968B2

公开(公告)日：2021-06-29

申请号：US16006485

申请日：2018-06-12

Applicant: Dow AgroSciences LLC ,  DSM IP Assets B.V.

Inventor： Terence A. Walsh ,  Daniel J. Gachotte ,  Cory M. Larsen ,  Scott Bevan ,  P. Ann Owens-Merlo ,  James G. Metz ,  Ross Zirkle

IPC: C12N15/82 ,  A23D9/00 ,  C12N9/12 ,  A23K20/158 ,  C11B1/00 ,  C11B1/04 ,  C11B1/10 ,  C12N9/10

Abstract: This disclosure concerns recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US10793837B2

公开(公告)日：2020-10-06

申请号：US15579751

申请日：2016-06-06

Applicant: DSM IP Assets B.V.

Inventor： James G. Metz ,  Jun Wang ,  Bastien Chevreux ,  Ross E. Zirkle ,  Anne-Cecile Bayne ,  James Casey Lippmeier

IPC: C12N15/82 ,  C12N9/10 ,  C12N9/00 ,  A23K20/158 ,  A23L33/115 ,  C12P7/64

Abstract: This disclosure concerns a novel modular docosahexaenoic acid (DHA) synthase and recombinant host organisms genetically modified with such synthase and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US20180282711A1

公开(公告)日：2018-10-04

申请号：US16006485

申请日：2018-06-12

Applicant: Dow AgroSciences LLC ,  DSM IP Assets B.V.

Inventor： Terence A. Walsh ,  Daniel J. Gachotte ,  Cory M. Larsen ,  Scott Bevan ,  P. Ann Owens-Merlo ,  James G. Metz ,  Ross Zirkle

IPC: C12N9/10 ,  C12N15/82 ,  C12N9/12 ,  C11B1/04 ,  C11B1/00 ,  A23K20/158 ,  A23D9/00 ,  C11B1/10

Abstract: This disclosure concerns recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US09994828B2

公开(公告)日：2018-06-12

申请号：US14555326

申请日：2014-11-26

Applicant: Dow AgroSciences LLC ,  DSM IP Assets B.V.

Inventor： Terence A. Walsh ,  Daniel J. Gachotte ,  Cory M. Larsen ,  Scott Bevan ,  P. Ann Owens-Merlo ,  James G. Metz ,  Ross Zirkle

IPC: C12N9/10 ,  C12N15/82 ,  A23D9/00 ,  C11B1/00 ,  C11B1/04 ,  C11B1/10 ,  A23K20/158 ,  C12N9/12

CPC classification number: C12N9/1029 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11B1/04 ,  C11B1/10 ,  C12N9/1288 ,  C12N15/82 ,  C12N15/8247 ,  C12Y203/01085

Abstract: This disclosure concerns recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US20150299676A1

公开(公告)日：2015-10-22

申请号：US14555326

申请日：2014-11-26

Applicant: DOW AGROSCIENCES LLC ,  DSM IP ASSETS B.V.

Inventor： Terence A. Walsh ,  Daniel J. Gachotte ,  Cory M. Larsen ,  Scott Bevan ,  P. Ann Owens-Merlo ,  James G. Metz ,  Ross Zirkle

IPC: C12N9/10 ,  C11B1/10 ,  C11B1/04 ,  C11B1/00

CPC classification number: C12N9/1029 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11B1/04 ,  C11B1/10 ,  C12N9/1288 ,  C12N15/82 ,  C12N15/8247 ,  C12Y203/01085

Abstract: This disclosure concerns recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

Abstract translation: 本公开涉及用多不饱和脂肪酸（PUFA）合酶系统遗传修饰的重组宿主生物体，以及允许和/或改善宿主生物体中PUFA生产的一种或多种辅助蛋白质。 本公开还涉及制造和使用这些生物以及从这些生物获得的产品的方法。

公开(公告)号：US20130295607A1

公开(公告)日：2013-11-07

申请号：US13923481

申请日：2013-06-21

Applicant: DSM IP Assets B.V.

Inventor： Paul G. ROESSLER ,  T. Dave Matthews ,  Tom M. Ramseier ,  James G. Metz

IPC: C12N15/82

CPC classification number: C12N15/82 ,  C12N9/88 ,  C12N15/79 ,  C12P21/02 ,  C12Y202/01006

Abstract: Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.

Abstract translation: 公开了乙酰乳酸合酶，乙酰乳酸合成酶调节区，α-微管蛋白启动子，破囊壶菌聚酮合成酶（PKS）系统的启动子和来自破囊壶菌菌体的脂肪酸去饱和酶启动子的核酸和氨基酸序列。 还公开了可用于转录破囊索杆菌微生物的重组载体，以及转变破囊壶菌微生物的方法。 本发明的重组核酸分子可用于在破囊壶菌属微生物中表达外源核酸，以及用于在裂囊酵素微生物中的基因的缺失，突变或失活。