公开(公告)号：US20090178935A1

公开(公告)日：2009-07-16

申请号：US12296639

申请日：2007-03-29

申请人： Frederic Reymond ,  Joel Stephane Rossier ,  Patrick Morier

发明人： Frederic Reymond ,  Joel Stephane Rossier ,  Patrick Morier

IPC分类号： G01N27/26

CPC分类号： G01N27/3273

摘要： A method to optimize the amperometric detection in a microsystem consists in limiting the detection to times when the diffusion layer (18-20) of the analyte to detect remains smaller than the microchannel (7) height. The charge detected during the second part of the amperometric measurement (which corresponds to the integral of the measured current over the corresponding time period) can also be considered so as to remove the contribution of the capacitive current and, when applicable, of the current resulting from the reduction or oxidation of the analyte molecules present in a recess above the electrode at the beginning of the detection. A microfluidic amperometric sensor for performing the method comprises at least one microchannel (7) having at least one electrode (15-17), integrated in one wall of the microchannel, and having a characteristic length or radius which is smaller than half the microchannel height.

摘要翻译： 在微系统中优化电流检测的方法包括将检测限于待检测的分析物的扩散层（18-20）保持小于微通道（7）高度的时间。 在电流分析测量的第二部分期间检测到的电荷（其对应于相应时间段内的测量电流的积分）也可以被考虑，以消除电容电流的贡献，并且在适用时消除所产生的电流 从在检测开始时存在于电极上方的凹槽中的分析物分子的还原或氧化。 用于执行该方法的微流体电流计传感器包括至少一个微通道（7），其具有集成在微通道的一个壁中的至少一个电极（15-17），并且具有小于微通道高度的一半的特征长度或半径 。

公开(公告)号：US07615354B2

公开(公告)日：2009-11-10

申请号：US10275041

申请日：2001-05-04

申请人： Michel D. Faupel ,  Hubert H. Girault ,  Frederic Reymond ,  Alexandra Ros ,  Joel Stephane Rossier

发明人： Michel D. Faupel ,  Hubert H. Girault ,  Frederic Reymond ,  Alexandra Ros ,  Joel Stephane Rossier

IPC分类号： G01N33/53

CPC分类号： G01N27/44795 ,  G01N27/44769 ,  G01N2030/0035 ,  G01N2030/285

摘要： A device, method and kit for the electrophoretic separation and purification of charged and neutral compounds in an analyte solution. The device comprises a chamber (1), at least one wall of which is composed of a chemical buffering system (4). A potential difference is applied across the buffering system, resulting in the charged and neutral compounds being differentially separated by extraction of the charged compounds into the buffering system. The device also comprises means for collecting the separated compounds, preferably in ampholyte-free or buffer-free solution and optionally means for recycling the separated fractions.

摘要翻译： 用于在分析物溶液中电荷分离和纯化带电和中性化合物的装置，方法和试剂盒。 该装置包括室（1），其至少一个壁由化学缓冲系统（4）组成。 在缓冲系统上施加电位差，导致带电和中性化合物通过将带电荷的化合物萃取到缓冲系统中而被差异地分离。 该装置还包括用于收集分离的化合物的装置，优选以不含两性电解质或不含缓冲液的溶液和用于再循环分离的级分的方法。

公开(公告)号：US20070240986A1

公开(公告)日：2007-10-18

申请号：US11718774

申请日：2005-11-11

申请人： Frederic Reymond ,  Joel Rossier ,  Patrick Morier

发明人： Frederic Reymond ,  Joel Rossier ,  Patrick Morier

IPC分类号： G01N27/26 ,  B23P15/00

CPC分类号： G01N33/5438 ,  B01L3/5027 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2300/0825 ,  B01L2400/0406 ,  C12Q1/001 ,  Y10T29/53204

摘要： An electrochemical microfluidic device has one or a plurality of microstructures, such as a microchannel, in which an electrically conductive means is integrated to reduce the ohmic resistance within the microstructure and hence to improve electrochemical measurements particularly when large current densities are involved. The electrically conductive means can be connected as a counter-electrode and can be used to re-generate the product of the reaction occurring at the working electrode. A method of fabricating electrochemical microfluidic devices comprising such an electrically conductive means is also disclosed. The invention may particularly be used in all electrochemical sensor applications where detection is performed in small volumes.

摘要翻译： 电化学微流体装置具有一个或多个微结构，例如微通道，其中整合了导电装置以降低微结构内的欧姆电阻，因此特别是当涉及大电流密度时，改善电化学测量。 导电装置可以作为对电极连接，并且可用于重新产生在工作电极处发生的反应的产物。 还公开了一种制造包括这种导电装置的电化学微流体装置的方法。 本发明可以特别用于所有电化学传感器应用中，其中以小体积进行检测。

公开(公告)号：US20060141469A1

公开(公告)日：2006-06-29

申请号：US10543762

申请日：2004-01-14

申请人： Joel Rossier ,  Frederic Reymond ,  Patrick Morier

发明人： Joel Rossier ,  Frederic Reymond ,  Patrick Morier

IPC分类号： C12Q1/68 ,  C12M1/34 ,  G01N33/558

CPC分类号： B01L3/502707 ,  B01L2300/0645 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  G01N27/333

摘要： The present invention relates to a microfluidic electrochemical sensor apparatus and a method for conducting analytical tests with said apparatus for multi-reactant assays. The apparatus of this invention is a multi-layer body made of at least three layers, the first one being a polymer layer (1) comprising a microstructure (5) with at least one integrated microelectrode (4) and conductive tracks (13) for connection to an external electrochemical unit, the second one being a non-porous material serving to cover said microstructure so as to enable microfluidic manipulations and the third one being a porous layer (2) such as a membrane or a glass frit, said porous layer comprising at least one reagent (3) to be solubilized upon contact with a test solution (7) and reacting with an analyte (6) present in said solution to form a product that is transported along said microstructure so as to enable electrochemical detection of said analyte. The invention notably enables the performance of multi-reactant assays in a reduced number of steps.

摘要翻译： 本发明涉及微流体电化学传感器装置和用于多反应物测定的所述装置进行分析测试的方法。 本发明的装置是由至少三层制成的多层体，第一层是包括具有至少一个集成微电极（4）的微结构（5）和导电轨道（13）的聚合物层（1），用于 连接到外部电化学单元，第二个是用于覆盖所述微结构的无孔材料，以便能够进行微流体操作，第三个是多孔层（2），例如膜或玻璃料，所述多孔层 包括至少一种试剂（3），其在与测试溶液（7）接触时溶解并与存在于所述溶液中的分析物（6）反应以形成沿着所述微结构传送的产物，以便能够电化学检测所述 分析物。 本发明特别地能够以减少数量的步骤进行多反应物测定。

公开(公告)号：US20070039835A1

公开(公告)日：2007-02-22

申请号：US10571986

申请日：2004-09-15

申请人： Joel Rossier ,  Patrick Morier ,  Frederic Reymond

发明人： Joel Rossier ,  Patrick Morier ,  Frederic Reymond

IPC分类号： G01F1/64 ,  G01N33/487

CPC分类号： B01L3/50273 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2400/0457 ,  B01L2400/0487 ,  G01F1/56 ,  G01F1/64

摘要： An electrochemical flow monitoring device comprises a microfluidic system comprising at least one covered microchannel (3) having an inlet (4) and an outlet (5). A pressure difference is applied between the inlet and the outlet of the microfluidic system, for example by changing the relative heights of the inlet (4) and outlet (5), such as to generate a flow of solution within the microchannel (3). The microfluidic system has at least one electrode (8) for monitoring said flow of solution by measuring an electrochemical property of said solution.

摘要翻译： 电化学流量监测装置包括微流体系统，其包括具有入口（4）和出口（5）的至少一个覆盖的微通道（3）。 在微流体系统的入口和出口之间施加压力差，例如通过改变入口（4）和出口（5）的相对高度，例如在微通道（3）内产生溶液流。 微流体系统具有至少一个用于通过测量所述溶液的电化学性质来监测溶液流的电极（8）。

公开(公告)号：US20060207877A1

公开(公告)日：2006-09-21

申请号：US11410106

申请日：2006-04-25

申请人： Walter Schmidt ,  Frederic Reymond ,  Joel Rossier

发明人： Walter Schmidt ,  Frederic Reymond ,  Joel Rossier

IPC分类号： G01N27/26 ,  B32B9/04

CPC分类号： B32B9/04 ,  B32B3/266 ,  B32B15/08 ,  B32B27/08 ,  B32B27/16 ,  B32B2307/206 ,  B32B2307/728 ,  B32B2307/73 ,  B32B2535/00 ,  Y10T428/31504

摘要： The technology is based on the plasma etching of organic polymer sheets partially protected by a metallic mask. The originality of the process is to pattern the surface properties by the same physical means as the one used for the three dimensional fabrication and simultaneously to this fabrication. Surface properties mean, but are not limited to hydrophobicity hydrophilicity, conductivity, reflectability, rugosity and more precisely the chemical and/or physical state of the surface. It is also possible to generate the desired fonctionalities, for instance carboxylic acid, ester, ether, amid or imide, during the etching process. The patterning of the different properties may be achieved by two different techniques that may be used separately or simultaneously.

摘要翻译： 该技术基于部分被金属掩模保护的有机聚合物片的等离子体蚀刻。 该方法的原创性是通过与用于三维制造的物理手段相同的物理手段来模拟表面性质，并同时进行该制造。 表面性质意味着但不限于疏水性亲水性，导电性，反射性，粗糙度，更准确地说是表面的化学和/或物理状态。 在蚀刻过程中也可以产生所需的功能，例如羧酸，酯，醚，酰胺或酰亚胺。 不同性质的图案化可以通过可以单独使用或同时使用的两种不同技术来实现。

公开(公告)号：US07037417B2

公开(公告)日：2006-05-02

申请号：US09812065

申请日：2001-03-19

申请人： Joel S. Rossier ,  Frederic Reymond ,  Hye Jin Lee ,  Xiaoxia Bai ,  Hubert H. Girault

发明人： Joel S. Rossier ,  Frederic Reymond ,  Hye Jin Lee ,  Xiaoxia Bai ,  Hubert H. Girault

IPC分类号： G01N27/26

CPC分类号： B01L3/502738 ,  B01L3/50273 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0622 ,  G01N1/14 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44791 ,  Y10T137/86493 ,  Y10T137/87708

摘要： An apparatus for manipulating fluid samples comprises a micro-chip device having first and second covered channels, each channel having openings at both ends. The channels intersect to form a common intersection. Three of the openings of the channels are connected to a multi-port valve to control pressure in the device.

摘要翻译： 用于操纵流体样品的装置包括具有第一和第二被覆通道的微芯片装置，每个通道在两端具有开口。 通道相交形成一个共同的交点。 通道中的三个开口连接到多口阀，以控制装置中的压力。

公开(公告)号：US20060113463A1

公开(公告)日：2006-06-01

申请号：US10534301

申请日：2003-11-07

申请人： Joel Rossier ,  Frederic Reymond

发明人： Joel Rossier ,  Frederic Reymond

IPC分类号： H01J49/00

CPC分类号： H01J49/0018 ,  H01J49/167

摘要： The present invention relates to an apparatus to dispense a sample for subsequent electrospray ionisation (ESI) mass spectrometry (MS) analysis, to a method of fabricating such apparatus and to applications of such apparatus in biological and chemical analysis. The apparatus consists of an electrically non-conductive substrate comprising at least two covered microstructures (generally microchannels) having one extremity formed at the edge of the substrate, one of said microstructures containing the sample to be dispensed into a mass spectrometer by electrospray ionisation and at least a second of said microstructure containing a fluid used as sheath liquid or sheath gas, said at least two microstrctures being formed in such a manner that the sample and the sheath liquid/gas come in contact to each other and/or are mixed directly in the Taylor cone of the spray.

摘要翻译： 本发明涉及一种用于随后的电喷雾离子化（ESI）质谱（MS）分析的样品的分配装置，涉及制造这种装置的方法以及这种装置在生物和化学分析中的应用。 该装置由包括至少两个覆盖的微结构（通常为微通道）的非导电衬底组成，其具有在衬底的边缘处形成的一个末端，所述微结构之一包含通过电喷雾电离分配到质谱仪中的样品 所述至少二分之一的所述微结构包含用作鞘液或鞘气的流体，所述至少两个微结构以这样的方式形成，使得样品和鞘液/气彼此接触和/或直接混合在 泰勒锥的喷雾。