GENERATION OF HIGH POLYHYDROXYBUTRATE PRODUCING OILSEEDS
    1.
    发明申请
    GENERATION OF HIGH POLYHYDROXYBUTRATE PRODUCING OILSEEDS 有权
    生产油脂的高聚羟基树脂的生成

    公开(公告)号:US20120180162A1

    公开(公告)日:2012-07-12

    申请号:US13395702

    申请日:2010-09-15

    CPC分类号: C12N15/8214 C12N15/8257

    摘要: Transgenic plants, plant material, plant cells, and genetic constructs for synthesis of biopolymers, for example polyhydroxyalkanoates (“PHA”) are provided. In one embodiment, the transgenic plants synthesize polyhydroxybutyrate (“PHB”). In one embodiment the transgenic plant encodes siRNA for one or more of the genes encoding enzymes for producing PHA. In a more preferred embodiment, the siRNA expression is under the control of an inducible regulatory element. In another embodiment, the transgenic plant contains transgenes that encode expression enzymes that will degrade the polymer. In a preferred embodiment, the expression of these enzymes is under the control of a germination specific, inducible, or minimal promoter. In another embodiment, the transgenic plant contains transgenes encoding enzymes that increase carbon flow for polymer synthesis. In a preferred embodiment, these transgenes encode enzymes that increase carbon flow in the Calvin Cycle.

    摘要翻译: 提供了用于合成生物聚合物的转基因植物,植物材料,植物细胞和遗传构建体,例如聚羟基链烷酸酯(“PHA”)。 在一个实施方案中,转基因植物合成聚羟基丁酸酯(“PHB”)。 在一个实施方案中,转基因植物编码编码用于产生PHA的酶的一种或多种基因的siRNA。 在更优选的实施方案中,siRNA表达受诱导型调节元件的控制。 在另一个实施方案中,转基因植物含有编码将降解聚合物的表达酶的转基因。 在优选的实施方案中,这些酶的表达受发芽特异性,诱导型或最小启动子的控制。 在另一个实施方案中,转基因植物含有编码增加聚合物合成碳流的酶的转基因。 在优选的实施方案中,这些转基因编码增加卡尔文循环中碳流的酶。

    Generation of high polyhydroxybutyrate producing oilseeds
    2.
    发明授权
    Generation of high polyhydroxybutyrate producing oilseeds 有权
    生产高聚羟基丁酸酯油籽油

    公开(公告)号:US09181559B2

    公开(公告)日:2015-11-10

    申请号:US13395702

    申请日:2010-09-15

    CPC分类号: C12N15/8214 C12N15/8257

    摘要: Transgenic plants, plant material, plant cells, and genetic constructs for synthesis of biopolymers, for example polyhydroxyalkanoates (“PHA”) are provided. In one embodiment, the transgenic plants synthesize polyhydroxybutyrate (“PHB”). In one embodiment the transgenic plant encodes siRNA for one or more of the genes encoding enzymes for producing PHA. In a more preferred embodiment, the siRNA expression is under the control of an inducible regulatory element. In another embodiment, the transgenic plant contains transgenes that encode expression enzymes that will degrade the polymer. In a preferred embodiment, the expression of these enzymes is under the control of a germination specific, inducible, or minimal promoter. In another embodiment, the transgenic plant contains transgenes encoding enzymes that increase carbon flow for polymer synthesis. In a preferred embodiment, these transgenes encode enzymes that increase carbon flow in the Calvin Cycle.

    摘要翻译: 提供了用于合成生物聚合物的转基因植物,植物材料,植物细胞和遗传构建体,例如聚羟基链烷酸酯(“PHA”)。 在一个实施方案中,转基因植物合成聚羟基丁酸酯(“PHB”)。 在一个实施方案中,转基因植物编码编码用于产生PHA的酶的一种或多种基因的siRNA。 在更优选的实施方案中,siRNA表达受诱导型调节元件的控制。 在另一个实施方案中,转基因植物含有编码将降解聚合物的表达酶的转基因。 在优选的实施方案中,这些酶的表达受发芽特异性,诱导型或最小启动子的控制。 在另一个实施方案中,转基因植物含有编码增加聚合物合成碳流的酶的转基因。 在优选的实施方案中,这些转基因编码增加卡尔文循环中碳流的酶。

    GREEN PROCESS AND COMPOSITIONS FOR PRODUCING POLY(5HV) AND 5 CARBON CHEMICALS
    8.
    发明申请
    GREEN PROCESS AND COMPOSITIONS FOR PRODUCING POLY(5HV) AND 5 CARBON CHEMICALS 有权
    用于生产聚(5HV)和5碳化合物的绿色方法和组合物

    公开(公告)号:US20100168481A1

    公开(公告)日:2010-07-01

    申请号:US12637706

    申请日:2009-12-14

    摘要: Recombinant hosts for producing polyhydroxyalkanoates and methods of producing polyhydroxyalkanoates from renewable carbon substrates are provided. Certain recombinant hosts that produce 5 carbon chemicals such as 5-aminopentanoate (5AP), 5-hydroxyvalerate (5HV), glutarate, and 1,5 pentanediol (PDO) are also provided. One embodiment provides a recombinant host expressing a gene encoding a heterologous enzyme selected from the group consisting of a polyhydroxyalkanoate synthase and a 5-hydroxyvalerate-CoA (5HV-CoA) transferase, wherein the host produces a polymer containing 5-hydroxyvalerate. Preferably, the host expresses both a polyhydroxyalkanoate synthase and a 5HV-CoA transferase. The host can be prokaryotic or eukaryotic. A preferred prokaryotic host is E. coli. The polymers produced by the recombinant hosts can be homopolymers or copolymers of 5-hydroxyvalerate. A preferred copolymer is poly(3-hydroxybutyrate-co-5-hydroxyvalerate).

    摘要翻译: 提供了用于生产聚羟基链烷酸酯的重组宿主和从可再生碳底物生产聚羟基链烷酸酯的方法。 还提供了产生5种碳化学物质的某些重组宿主,如5-氨基戊酸(5AP),5-羟基戊酸酯(5HV),戊二酸酯和1,5-戊二醇(PDO)。 一个实施方案提供了表达编码选自聚羟基链烷酸酯合酶和5-羟基戊酸酯-CoA(5HV-CoA)转移酶的异源酶的基因的重组宿主,其中所述宿主产生含有5-羟基戊酸酯的聚合物。 优选地,宿主表达聚羟基链烷酸酯合成酶和5HV-CoA转移酶。 宿主可以是原核生物或真核生物。 优选的原核宿主是大肠杆菌。 由重组宿主产生的聚合物可以是5-羟基戊酸酯的均聚物或共聚物。 优选的共聚物是聚(3-羟基丁酸酯 - 共-5-羟基戊酸酯)。