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    Cell lysis device and methods of lysing cells or viruses
    6.
    发明授权
    Cell lysis device and methods of lysing cells or viruses 有权
    细胞裂解装置和裂解细胞或病毒的方法

    公开(公告)号：US08986986B2

    公开(公告)日：2015-03-24

    申请号：US13284364

    申请日：2011-10-28

    申请人： Kyu-youn Hwang ,  Joon-ho Kim ,  Sung-hong Kwon ,  Chin-sung Park ,  Hee-kyun Lim ,  Sun-ok Jung ,  Won-jong Jung

    发明人： Kyu-youn Hwang ,  Joon-ho Kim ,  Sung-hong Kwon ,  Chin-sung Park ,  Hee-kyun Lim ,  Sun-ok Jung ,  Won-jong Jung

    IPC分类号： C12M1/33 ,  C12M3/08 ,  C12Q1/68 ,  C12Q1/70 ,  C12M3/00 ,  C12M1/00

    CPC分类号： C12M1/00 ,  C12M1/33 ,  C12M47/06

    摘要： A method of lysing at least one of a cell and a virus, the method including: contacting a sample, which includes at least one of a cell and a virus, with a plurality of beads which are disposed in a first chamber to obtain a combination of the sample and the beads; and agitating the combination of the sample and the beads to lyse the at least one of the cell and the virus, wherein in the first chamber a liquid volume fraction is 0.6 or less, and wherein the liquid volume fraction is a value obtained by dividing a liquid volume of the first chamber by a pure void volume equivalent to a sum of the liquid volume of the first chamber and a void volume of the first chamber.

    摘要翻译： 一种裂解细胞和病毒中的至少一种的方法，所述方法包括：将包括细胞和病毒中的至少一种的样品与设置在第一室中的多个珠接触以获得组合 的样品和珠粒; 并搅拌样品和珠的组合以裂解细胞和病毒中的至少一种，其中在第一室中液体体积分数为0.6或更小，并且其中液体体积分数是通过将 第一室的液体体积相当于第一室的液体体积和第一室的空隙体积的总和的纯空隙体积。

    Fluorescence detecting optical system and multi-channel fluorescence detection apparatus including the same
    8.
    发明授权
    Fluorescence detecting optical system and multi-channel fluorescence detection apparatus including the same 有权
    荧光检测光学系统和包括其的多通道荧光检测装置

    公开(公告)号：US09000399B2

    公开(公告)日：2015-04-07

    申请号：US13271101

    申请日：2011-10-11

    申请人： Kyung-ho Kim ,  Joon-ho Kim ,  Kak Namkoong ,  Won-seok Chung

    发明人： Kyung-ho Kim ,  Joon-ho Kim ,  Kak Namkoong ,  Won-seok Chung

    IPC分类号： G01N21/64 ,  G01J3/44 ,  G01J3/02 ,  G01N21/05 ,  G01N21/03

    CPC分类号： G01N21/645 ,  G01J3/0208 ,  G01J3/0229 ,  G01J3/0237 ,  G01J3/4406 ,  G01N21/05 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6478 ,  G01N2201/0635

    摘要： A fluorescence detection optical system detects fluorescence beams with two or more different wavelengths and maintains a focal position through an automatic focusing function. A multi-channel fluorescence detection apparatus includes the fluorescence detection optical system. The fluorescence detection optical system includes an automatic focusing unit which receives light reflected off a microfluidic device and determines a focal point by using an astigmatic method or a knife edge method, and an actuator which adjusts a position of an objective lens according to control of the automatic focusing unit. In addition, the fluorescence detection optical system may include a plurality of dual band pass filters, dichroic devices, etc., which provide light beams emitted from at least two light sources and transfer fluorescence generated from the microfluidic device to a photodetector.

    摘要翻译： 荧光检测光学系统检测具有两个或更多个不同波长的荧光束并通过自动聚焦功能保持焦点位置。 多通道荧光检测装置包括荧光检测光学系统。 荧光检测光学系统包括自动聚焦单元，其接收从微流体装置反射的光并且通过使用散光方法或刀刃方法确定焦点，以及致动器，其根据控制的方式调整物镜的位置 自动对焦单元 此外，荧光检测光学系统可以包括多个双带通滤波器，二向色装置等，其提供从至少两个光源发射的光束并将从微流体装置产生的荧光转移到光电检测器。

    Polymerase chain reaction apparatus
    9.
    发明授权
    Polymerase chain reaction apparatus 有权
    聚合酶链反应装置

    公开(公告)号：US08663976B2

    公开(公告)日：2014-03-04

    申请号：US13039630

    申请日：2011-03-03

    申请人： Won-seok Chung ,  Kak Namkoong ,  Joon-ho Kim

    发明人： Won-seok Chung ,  Kak Namkoong ,  Joon-ho Kim

    IPC分类号： C12M1/34 ,  C12M3/00

    CPC分类号： B01L9/527 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/147 ,  B01L2300/1827

    摘要： A polymerase chain reaction (“PCR”) apparatus includes a PCR chip holder which accommodates and supports a PCR chip, a housing which supports ends of the PCR chip holder, a temperature control element which moves perpendicularly with respect to the PCR chip holder in a space between the housing and the PCR chip holder in the housing, and an elastic unit which elastically biases the temperature control element toward the PCR chip holder, between the temperature control element and the housing. The temperature control element includes a top surface which is selectively contacted to a bottom surface of the PCR chip.

    摘要翻译： 聚合酶链式反应（“PCR”）装置包括：一个PCR芯片保持器，其容纳和支持PCR芯片;支撑PCR芯片保持器端部的壳体;一个相对于PCR芯片保持器垂直移动的温度控制元件 壳体和壳体中的PCR芯片保持器之间的空间以及在温度控制元件和壳体之间弹性地将温度控制元件朝向PCR芯片保持器偏置的弹性单元。 温度控制元件包括选择性地接触PCR芯片的底表面的顶表面。

    Fluorescence detecting optical system and multi-channel fluorescence detection apparatus having the same
    10.
    发明授权
    Fluorescence detecting optical system and multi-channel fluorescence detection apparatus having the same 有权
    荧光检测光学系统和具有该荧光检测光学系统的多通道荧光检测装置

    公开(公告)号：US08624201B2

    公开(公告)日：2014-01-07

    申请号：US13421119

    申请日：2012-03-15

    申请人： Kyung-ho Kim ,  Joon-ho Kim ,  Kak Namkoong ,  Won-seok Chung

    发明人： Kyung-ho Kim ,  Joon-ho Kim ,  Kak Namkoong ,  Won-seok Chung

    IPC分类号： G01N21/64

    CPC分类号： G01N21/645 ,  G01N21/6445

    摘要： A fluorescence detecting optical system, and a multi-channel fluorescence detection apparatus comprising same, comprising a light source that emits excitation light, a polarizer that transmits light having a predetermined polarization component, a polarizing beam splitter that transmits light having a predetermined polarization component and reflects light having a different polarization component than the predetermined polarization component, and a quarter-wave plate converting linearly polarized to circularly polarized light or vice versa.

    摘要翻译： 一种荧光检测光学系统和包括其的多通道荧光检测装置，包括发射激发光的光源，透射具有预定偏振分量的光的偏振器，透射具有预定偏振分量的光的偏振分束器和 反射具有与预定偏振分量不同的偏振分量的光，以及将线偏振成圆偏振光的四分之一波片反射。