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182 条记录 (耗时 0.041 秒)

    Amplification of nucleic acids
    1.
    发明授权
    Amplification of nucleic acids 有权
    核酸扩增

    公开(公告)号：US06582938B1

    公开(公告)日：2003-06-24

    申请号：US09854317

    申请日：2001-05-11

    申请人： Xing Su ,  Helin Dong ,  Thomas B. Ryder

    发明人： Xing Su ,  Helin Dong ,  Thomas B. Ryder

    IPC分类号： C12P1934

    CPC分类号： C12Q1/6865

    摘要： The present invention relates to the amplification of nucleic acids, preferably from mRNA. A primer and promoter are added to a target sequence to be amplified and then the target is amplified in an in vitro transcription reaction.

    摘要翻译： 本发明涉及核酸，优选mRNA的扩增。 将引物和启动子加入要扩增的靶序列中，然后在体外转录反应中扩增靶。

    Chemically induced optical signals and DNA sequencing
    2.
    发明授权
    Chemically induced optical signals and DNA sequencing 有权

    公开(公告)号：US09671558B2

    公开(公告)日：2017-06-06

    申请号：US12459309

    申请日：2009-06-30

    申请人： Xing Su ,  Liming Wang ,  Jianquan Liu ,  Kai Wu

    发明人： Xing Su ,  Liming Wang ,  Jianquan Liu ,  Kai Wu

    IPC分类号： C12Q1/68 ,  G02B6/122

    CPC分类号： G02B6/1221 ,  C12Q1/6874 ,  C12Q2565/607 ,  C12Q2563/107 ,  C12Q2527/125

    摘要： Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and optically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced.

    Enzymatic signal generation and detection of binding complexes in stationary fluidic chip
    4.
    发明授权
    Enzymatic signal generation and detection of binding complexes in stationary fluidic chip 有权
    在固定流体芯片中的酶信号产生和结合复合物的检测

    公开(公告)号：US08664008B2

    公开(公告)日：2014-03-04

    申请号：US13543488

    申请日：2012-07-06

    申请人： David J. Liu ,  Kai Wu ,  Xing Su

    发明人： David J. Liu ,  Kai Wu ,  Xing Su

    IPC分类号： G01N33/553 ,  C12Q1/00

    CPC分类号： G01N21/658 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2300/0654 ,  B01L2400/043 ,  B01L2400/086 ,  B82Y15/00 ,  B82Y30/00 ,  G01N21/255 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/76 ,  G01N33/54333 ,  G01N33/587

    摘要： An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

    摘要翻译： 本发明的一个实施例涉及用于检测样品中的分析物的装置。 该装置包括流体网络和集成电路部件。 流体网络包括样品区，清洁区和检测区。 流体网络包含磁性颗粒和/或信号颗粒。 引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。 微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。 存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

    Enzymatic signal generation and detection of binding complexes in stationary fluidic chip
    5.
    发明授权
    Enzymatic signal generation and detection of binding complexes in stationary fluidic chip 有权
    在固定流体芯片中的酶信号产生和结合复合物的检测

    公开(公告)号：US08409877B2

    公开(公告)日：2013-04-02

    申请号：US11647565

    申请日：2006-12-29

    申请人： David J. Liu ,  Kai Wu ,  Xing Su

    发明人： David J. Liu ,  Kai Wu ,  Xing Su

    IPC分类号： G01N33/553 ,  C12Q1/00

    CPC分类号： G01N21/658 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2300/0654 ,  B01L2400/043 ,  B01L2400/086 ,  B82Y15/00 ,  B82Y30/00 ,  G01N21/255 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/76 ,  G01N33/54333 ,  G01N33/587

    摘要： An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

    摘要翻译： 本发明的一个实施例涉及用于检测样品中的分析物的装置。 该装置包括流体网络和集成电路部件。 流体网络包括样品区，清洁区和检测区。 流体网络包含磁性颗粒和/或信号颗粒。 引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。 微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。 存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

    Solid-phase chelators and electronic biosensors
    6.
    发明申请
    Solid-phase chelators and electronic biosensors 有权
    固相螯合剂和电子生物传感器

    公开(公告)号：US20110159481A1

    公开(公告)日：2011-06-30

    申请号：US12655459

    申请日：2009-12-30

    申请人： David J. Liu ,  Xing Su ,  Kai Wu

    发明人： David J. Liu ,  Xing Su ,  Kai Wu

    IPC分类号： C12Q1/68 ,  G01N33/50 ,  G01N27/26

    CPC分类号： C12Q1/48 ,  C12Q1/6837 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N2333/9125 ,  C12Q2565/607 ,  C12Q2565/507 ,  C12Q2565/301

    摘要： Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and electronically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced. Additionally, devices and methods are provided for detecting biomolecules through the detection of pyrophosphate ions.

    摘要翻译： 介绍了核酸测序方法。 通过化学扩增DNA合成产物和化学扩增产物的检测来完成测序。 在本发明的实施方案中，提供了具有多个待测序的DNA分子的底物和能够螯合连接的焦磷酸根离子的多个分子，引入待测序的DNA分子，并且掺入下一个互补核苷酸， 切除多次导致待测序的DNA分子局部周围的焦磷酸盐离子的积聚。 焦磷酸根离子被底物连接的螯合剂捕获并电子检测以确定待测序的DNA分子中下一个互补核酸的同一性。 另外，提供了通过检测焦磷酸根离子来检测生物分子的装置和方法。

    Programmable electromagnetic array for molecule transport
    8.
    发明授权
    Programmable electromagnetic array for molecule transport 有权
    用于分子运输的可编程电磁阵列

    公开(公告)号：US07820454B2

    公开(公告)日：2010-10-26

    申请号：US11647578

    申请日：2006-12-29

    申请人： Xing Su ,  Kenneth Swartz ,  Liming Wang ,  David Eric Schwartz ,  Mineo Yamakawa

    发明人： Xing Su ,  Kenneth Swartz ,  Liming Wang ,  David Eric Schwartz ,  Mineo Yamakawa

    IPC分类号： G01N33/553

    CPC分类号： B01L3/502761 ,  B01F11/0266 ,  B01F13/0059 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/043 ,  B01L2400/0439 ,  B01L2400/0677

    摘要： An embodiment of the invention relates to a device comprising (1) an array of electromagnetic elements comprising coils, metal cores, and metal core heads, and (2) a controller that is adapted to control a current for one or more coils individually, to vary the current for said one or more coils individually, to reverse the current for one or more coils individually, and to generate a specific magnetic flux distribution and gradient across two or more coils; wherein the metal core head is at one end of the coil and the metal core head has a geometry to create a desired magnetic flux, intensity and gradient, in a region of interest between two adjacent coils; further wherein the device is functionally coupled to a fluidic device to concentrate and transport magnetic particles in a fluid without fluidic movement of the fluid.

    摘要翻译： 本发明的实施例涉及一种装置，包括（1）包括线圈，金属芯和金属芯头的电磁元件阵列，以及（2）适于一个或多个线圈分别控制电流的控制器， 单独地改变所述一个或多个线圈的电流，以单独地反转一个或多个线圈的电流，并且跨越两个或更多个线圈产生特定的磁通分布和梯度; 其中所述金属芯头位于所述线圈的一端，并且所述金属芯头具有几何形状以在两个相邻线圈之间的感兴趣区域中产生期望的磁通量，强度和梯度; 其中所述装置在功能上耦合到流体装置以将流体中的磁性颗粒集中并输送到流体中而不流体流动。

    Aptamer biochip for multiplexed detection of biomolecules
    9.
    发明申请
    Aptamer biochip for multiplexed detection of biomolecules 审中-公开
    适用于生物分子多重检测的生物芯片

    公开(公告)号：US20100240544A1

    公开(公告)日：2010-09-23

    申请号：US11529554

    申请日：2006-09-29

    申请人： David J. Liu ,  Xing Su

    发明人： David J. Liu ,  Xing Su

    IPC分类号： C40B30/04 ,  C07H21/04 ,  C12P19/34 ,  C40B40/08

    CPC分类号： C12Q1/6837 ,  C12Q2543/101 ,  C12Q2525/205 ,  C12Q2525/143

    摘要： The embodiments of the invention relate to an in situ generated and self-addressed aptamer biochip for the multiplexed detection of biomolecules. The inventive aptamer biochip uses sets of complementary probes to permit in situ generation and immobilization of aptamers on the aptamer biochip surface to form an addressable aptamer array. These aptamer biochip arrays can be used for detecting multiple biomolecules, especially those for disease signature pattern analysis.

    摘要翻译： 本发明的实施方案涉及用于多重检测生物分子的原位产生和自寻址的适体生物芯片。 本发明的适体生物芯片使用一组互补探针，以允许适配体在适体生物芯片表面上原位产生和固定以形成可寻址的适体阵列。 这些适配体生物芯片阵列可用于检测多种生物分子，特别是用于疾病特征模式分析的生物分子。

    Cellular analysis using Raman surface scanning
    10.
    发明授权
    Cellular analysis using Raman surface scanning 有权
    使用拉曼表面扫描的细胞分析

    公开(公告)号：US07776547B2

    公开(公告)日：2010-08-17

    申请号：US11027470

    申请日：2004-12-30

    申请人： Mark Roth ,  Andrew Berlin ,  Selena Chan ,  Tae-Woong Koo ,  Xing Su ,  Lei Sun

    发明人： Mark Roth ,  Andrew Berlin ,  Selena Chan ,  Tae-Woong Koo ,  Xing Su ,  Lei Sun

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54366 ,  G01N33/54373 ,  G01N33/58 ,  Y10T436/10

    摘要： Methods and apparatus are provided for assaying cell samples, which may be living cells, using probes labeled with composite organic-inorganic nanoparticles (COINs) and microspheres with COINs embedded within a polymer matrix to which the probe moiety is attached. COINs intrinsically produce SERS signals upon laser irradiation, making COIN-labeled probes particularly suitable in a variety of methods for assaying cells, including biological molecules that may be contained on or within cells, most of which are not inherently Raman-active. The invention provides variations of the sandwich immunoassay employing both specific and degenerate binding, methods for reverse phase assay of tissue samples and cell microstructures, in solution displacement and competition assays, and the like. Systems and chips useful for practicing the invention assays are also provided.

    摘要翻译： 提供了用于使用用复合有机 - 无机纳米颗粒（COIN）标记的探针和嵌入探针部分所连接的聚合物基质内的COIN的微球来测定可能为活细胞的细胞样品的方法和装置。 COIN在激光照射下固有地产生SERS信号，使得COIN标记的探针特别适用于多种测定细胞的方法，包括可能包含在细胞内或细胞内的生物分子，其中大部分不是固有的拉曼活性的。 本发明提供使用特异性和简并结合的夹心免疫测定的变体，组织样品和细胞微结构的反相测定方法，溶液置换和竞争测定等。 还提供了用于实施本发明测定的系统和芯片。