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公开(公告)号：US5387109A
公开(公告)日：1995-02-07
申请号：US66499
申请日：1993-05-24
CPC分类号： C12N9/0012 , C12Q1/26
摘要： Disclosed are a novel fructosylamine deglycase characterized by the specificity to amadori compounds of catalyzing oxidation of the compounds to produce an .alpha.-ketoaldehyde, an amine derivative and hydrogen peroxide; a method of producing the novel enzyme by cultivating microorganisms belonging to the genus Candida and having an ability of producing the novel enzyme; and a method of quantitative determination of amadori compounds by applying the novel enzyme to a sample containing amadori compounds to measure the amount of the hydrogen peroxide to be formed by the oxidation reaction or measure the amount of the oxygen to be consumed by the reaction to thereby obtain the amount of the amadori compounds from the measured value. The invention provides a novel enzyme characterized by the high specificity to the reaction with amadori compounds, especially that having therein a saccharide moiety as bonded to the .epsilon.-amino group, and also provides a method of producing the enzyme and a method of quantitative determination of amadori compounds with the enzyme. Using the enzyme, quantitative determination of amadori compounds, which is difficult by conventional enzymatic methods, is possible with ease. In particular, in measurement of the amount of a saccharified protein in a sample from a living body, which is an important index substance in diagnosis of diabetes, the enzymatic method of the invention of measuring fructosamine in the sample is hardly influenced by other interfering substances or impurities in the sample, the influence by them having been inevitable in conventional chemical methods. Accurate determination of the amount is possible by the method, and the method is free from the inconvenience of staining the kits and instruments used. For measuring glycohemoglobin, the method displays another characteristic feature that its operation is more simple and needs less labor and time than conventional methods.
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2.发明授权Methods for protecting vegetables, turfgrass, rice and fruit trees from fungi and bacteria 失效从真菌和细菌中保护蔬菜，草坪草，水稻和果树的方法
公开(公告)号：US5374627A
公开(公告)日：1994-12-20
申请号：US006145
申请日：1993-01-19
申请人： Kazuhiro Ito , Etsuzo Entani , Yoshiya Kawamura
发明人： Kazuhiro Ito , Etsuzo Entani , Yoshiya Kawamura
摘要： A variety of plant diseases and damage by certain pests in agricultural and horticultural plants can be efficiently controlled without substantial chemical injuries by applying a plant-protecting composition which comprises (a) 1 part by weight of a chitosan hydrolyzate having an average molecular weight of 10,000 to 50,000, obtained by acid hydrolysis or enzymatic hydrolysis of chitosan and (b) 0.25 to 4 parts by weight of acetic acid. Any possible chemical injury to plants caused by applying the composition can be further reduced when the composition is admixed with a deproteinized juice of alfalfa leaves.
摘要翻译： 通过施用植物保护组合物可以有效地控制农业和园艺植物中的某些害虫的各种植物病害和损害，而不会造成实质的化学损伤，所述植物保护组合物包含（a）1重量份的平均分子量为10,000的壳聚糖水解产物 至50,000，通过壳聚糖的酸水解或酶水解获得，和（b）0.25至4重量份的乙酸。 当将组合物与脱蛋白的苜蓿叶汁混合时，通过施用组合物引起的对植物的任何可能的化学损伤可以进一步降低。
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公开(公告)号：US5085851A
公开(公告)日：1992-02-04
申请号：US552533
申请日：1990-07-16
CPC分类号： A61K8/66 , A61Q11/00 , Y10S514/90 , Y10S514/901 , Y10S514/902
摘要： A dental plaque-degrading composition containing endodextranase produced by Arthrobacter globiformis W31 exhibiting high degradation efficacy to insoluble glucan produced by Streptococcus mutans IFO 13955, the dental plaque-degrading composition additionally containing .alpha.-amylase of various origins. These compositions are useful for preventing the settlement of Streptococcus mutans in the oral cavity and the formation of new dental plaque and for preventing the dental caries, and provided in the form of dentifrices, denture detergents, troches, mouth washes, chewing gums or candies.
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公开(公告)号：US5909114A
公开(公告)日：1999-06-01
申请号：US957393
申请日：1997-10-23
CPC分类号： G01N27/423 , G01N33/1806
摘要： The present invention provides a rapid coulometric analysis for the quantitative determination of a sample substance with high reproducibility without any application of voltage etc. from the outside, as well as a galvanic cell and a device for use in said coulometric analysis. According to the present invention, a wide variety of components can be analyzed by selection of electroactive substance introduced into the galvanic cell. The present invention enables a simple and easy analysis for food components such as glutamic acid, ascorbic acid, etc., water quality such as COD, etc.
摘要翻译： 本发明提供了一种用于定量测定样品物质的快速库仑分析，其重现性高，而没有外部施加电压等，以及用于所述库仑分析的电池和装置。 根据本发明，可以通过选择引入到原电池中的电活性物质来分析各种各样的组分。 本发明能够简单易用地分析谷物酸，抗坏血酸等食品成分，COD等水质等。
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公开(公告)号：US4575551A
公开(公告)日：1986-03-11
申请号：US545270
申请日：1983-10-25
CPC分类号： C12R1/02 , A23L29/269 , C12P19/04 , Y10S435/823
摘要： Novel highly viscous acidic heterpolysaccharide AM-2 is produced from a strain of genus Acetobacter isolated from vinegar mash, and is useful as an adhesive, coating agent, drilling mud additive, enhanced oil recovering agent, etc., as well as a material useful for the production of foods, drugs and cosmetics because of its safety and high viscosity.
摘要翻译： 新型高度粘稠的酸性多糖AM-2是从醋醪分离出的醋杆菌属菌株产生的，可用作粘合剂，包衣剂，钻井泥浆添加剂，增强油回收剂等，以及可用于 由于其安全性和高粘度，食品，药品和化妆品的生产。
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公开(公告)号：US4514563A
公开(公告)日：1985-04-30
申请号：US479919
申请日：1983-03-29
CPC分类号： C12P19/04 , C08B37/006
摘要： Novel highly viscous polysaccharides composed mainly of (a) glucose, (b) galactose, (c) mannose, and (d) glucuronic acid, the molar ratio of (a):(b):(c):(d) being 10:3-6:0.5-2:0.5-2 (preferably 10:3-4:1-2:1-2). The invention also provides the process for producing the highly viscous polysaccharides which comprises cultivating a highly viscous polysaccharide-producing microorganism which is an acetic acid bacteria in a nutrient medium until a substantial amount of polysaccharides has accumulated in the nutrient medium, and recovering the accumulated polysaccharides from the nutrient medium.
摘要翻译： 主要由（a）葡萄糖，（b）半乳糖，（c）甘露糖和（d）葡萄糖醛酸组成的新型高粘度多糖，（a）:( b）：（c）：（d）的摩尔比为10 ：3-6：0.5-2：0.5-2（优选10：3-4：1-2：1-2）。 本发明还提供了生产高粘度多糖的方法，其包括在营养培养基中培养高粘度多糖产生微生物，其是乙酸菌，直到在营养培养基中积累大量多糖，并回收积聚的多糖 从营养介质。
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公开(公告)号：US4364960A
公开(公告)日：1982-12-21
申请号：US192468
申请日：1980-09-30
申请人： Yoshio Kunimatsu , Hajime Okumura , Hiroshi Masai , Koki Yamada , Mikio Yamada
发明人： Yoshio Kunimatsu , Hajime Okumura , Hiroshi Masai , Koki Yamada , Mikio Yamada
CPC分类号： C12J1/00
摘要： A finished vinegar having an acetic acid concentration higher than 20 percent weight by volume is produced by repeating a fermentation cycle wherein a broth is fermented at 27.degree.-32.degree. C. in a 1st submerged fermentation tank by a continuous batch process, and, when the acetic acid concentration of the fermenting broth reaches 12-15 percent weight by volume, the large part of the fermenting broth in the 1st fermentation tank is withdrawn and charged in a 2nd submerged fermentation tank. The 1st fermentation tank is recharged with a mash and the fermentation of the fermenting broth in the 2nd tank is continued under aeration while lowering the temperature of the fermenting broth in such manner that the final fermentation temperature does not fall below 18.degree. C. and the lowering temperature does not become higher than the temperature once lowered.
摘要翻译： 乙酸浓度高于20体积重量的成品醋通过重复发酵循环来制备，其中在第一浸没发酵罐中通过连续分批过程在27℃-32℃发酵肉汤，并且当 发酵液的乙酸浓度达到12-15重量％，第一发酵罐中大部分发酵液被取出并装入第二个浸没发酵罐中。 第一发酵罐用醪液补充，并且在通气的同时继续在第二罐中发酵发酵液，同时以最终发酵温度不低于18℃的方式降低发酵液的温度，并且 降低温度不会高于一旦降低的温度。
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公开(公告)号：US5726565A
公开(公告)日：1998-03-10
申请号：US511476
申请日：1995-08-04
申请人： Shunichi Uchiyama , Takeshi Sato , Hirofumi Akano , Yoshiya Kawamura , Masahiro Fukaya , Hiroaki Ebisuya , Ko Furukawa , Kazuyo Kajino , Sumio Akita
发明人： Shunichi Uchiyama , Takeshi Sato , Hirofumi Akano , Yoshiya Kawamura , Masahiro Fukaya , Hiroaki Ebisuya , Ko Furukawa , Kazuyo Kajino , Sumio Akita
CPC分类号： G01N27/423 , G01N33/1806
摘要： The present invention provides a rapid coulometric analysis for the quantitative determination of a sample substance wherein the coulometric analysis has high reproducibility without any application of voltage, etc., from an outside source, as well as a galvanic cell and a device for use in said coulometric analysis. According to the present invention, a wide variety of components can be analyzed by selection of electroactive substance introduced into the galvanic cell. The present invention enables a simple and easy analysis for food components such as glutamic acid, ascorbic acid, etc., water quality such as COD, etc.
摘要翻译： 本发明提供了用于定量测定样品物质的快速库仑分析，其中库仑分析具有高的再现性，而不需要外部来源的电压等的施加，以及用于所述的电池的电池和装置 库仑分析 根据本发明，可以通过选择引入到原电池中的电活性物质来分析各种各样的组分。 本发明能够简单易用地分析谷物酸，抗坏血酸等食品成分，COD等水质等。
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9.发明授权Structural gene of membrane-bound alcohol dehydrogenase complex, plasmid containing the same and transformed acetic acid bacteria 失效膜结合醇脱氢酶复合物的结构基因，含有相同的转化乙酸菌的质粒
公开(公告)号：US5344777A
公开(公告)日：1994-09-06
申请号：US985458
申请日：1992-12-03
申请人： Toshimi Tamaki , Hiroshi Takemura , Kenji Tayama , Masahiro Fukaya , Hajime Okumura , Yoshiya Kawamura
发明人： Toshimi Tamaki , Hiroshi Takemura , Kenji Tayama , Masahiro Fukaya , Hajime Okumura , Yoshiya Kawamura
IPC分类号： C12N9/04 , C07K16/40 , C12N1/21 , C12N15/09 , C12N15/53 , C12R1/01 , C12R1/02 , C12N15/31 , C12N15/74
CPC分类号： C07K16/40 , C12N9/0006 , Y10S435/823
摘要： There is provided a structural gene of membrane-bound alcohol dehydrogenase complex having a molecular size of about 7.0 kilo base which is derived from a microorganism belonging to the genus Acetobacter represented by Acetobacter altoacetigenes and shown by the nucleotide sequence of SEQ ID. NO. 1 and SEQ ID NO. 2. This enzyme increases the efficiency of acetic acid fermentation and may be effectively utilized for quantitative determination of alcohol.
摘要翻译： 本发明提供一种具有约7.0千碱基的膜结合醇脱氢酶复合物的结构基因，该结构基因来源于由醋杆菌属（Acetobacter altoacetigenes）代表的属于醋杆菌属的微生物，由SEQ ID NO： 没有。 1和SEQ ID NO： 这种酶提高了乙酸发酵的效率，可以有效地用于定量测定酒精。
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10.发明授权Electrophoretic medium for electrophoretic separation, gel holder for holding the same medium, slab type electrophoretic apparatus using the same medium and gel holder, and electrophoretic gel cutter 失效用于电泳分离的电泳介质，用于保持相同介质的凝胶保持器，使用相同介质和凝胶保持器的板式电泳装置，以及电泳凝胶切割器
公开(公告)号：US5340461A
公开(公告)日：1994-08-23
申请号：US12194
申请日：1993-02-02
申请人： Tomoaki Fukushige , Hiroko Toda , Hirofumi Akano , Emiko Ito , Tomohiko Fukuta , Masahiro Fujimori , Yoshiya Kawamura
发明人： Tomoaki Fukushige , Hiroko Toda , Hirofumi Akano , Emiko Ito , Tomohiko Fukuta , Masahiro Fujimori , Yoshiya Kawamura
IPC分类号： B01D57/02 , G01N27/447 , C25B9/00
CPC分类号： G01N27/44704 , B01D57/02 , G01N27/44747
摘要： An electrophoretic medium best suited as a separation medium is composed of a gel solution and contains a water-soluble polymer and permits great improvement of the enzymic protein recovery character. A gel holder is provided with stopper members and thus can hold even a soft or slippery while maintaining a fixed shape thereof, thus permitting reliable separation and purification of an intended substance without possibility of denaturing of the substance. A slab type electrophoretic apparatus permits automatic detection of the electrophoretic state of a sample and automatic completion of the electrophoresis under optimum conditions without setting measurement condition, permits continuous measurement of the buffer solution temperature, which is important for obtaining an electrophoretic condition for maintaining the enzymic function, and permits separation and purification of a large quantity of an enzyme or like physiologically active substance in a very simple operation. An electrophoretic gel cutter cuts even large size electrophoretic gels vary accurately and safely to desired sizes and particularly cuts a soft and readily deformable electrophoretic gel of polyacrylamide or the like in a pressurized state, thus permitting accurate and safe cutting of the polyacrylamide gel, which has been very difficult to cut, without causing damage to the gel.
摘要翻译： 最适合作为分离介质的电泳介质由凝胶溶液组成并含有水溶性聚合物，并且可大大提高酶蛋白质的恢复特性。 凝胶保持器设置有止动件，因此可以保持柔软或滑动，同时保持其固定形状，从而允许可预期的物质的可靠分离和纯化，而不会使物质变性。 板式电泳装置允许自动检测样品的电泳状态，并在最佳条件下自动完成电泳，而不设置测量条件，允许连续测量缓冲溶液温度，这对于获得维持酶的电泳条件是重要的 功能，并且允许在非常简单的操作中分离和纯化大量的酶或类似的生理活性物质。 电泳凝胶切割机甚至将大尺寸电泳凝胶切割成精确和安全地变化到所需尺寸，并且特别地在加压状态下切割聚丙烯酰胺等的柔软且容易变形的电泳凝胶，从而允许精确和安全地切割聚丙烯酰胺凝胶，其具有 非常难以切割，不会对凝胶造成伤害。
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