公开(公告)号：US11069054B2

公开(公告)日：2021-07-20

申请号：US15403940

申请日：2017-01-11

申请人： VISIONGATE, INC.

发明人： Alan C. Nelson ,  Michael G. Meyer ,  Daniel J. Sussman

IPC分类号： A61K31/167 ,  G06T7/00 ,  G01N33/483 ,  A61K31/5578 ,  A61K31/593 ,  G01N15/14 ,  G06K9/00 ,  G01N15/10

摘要： A method of treating a malignancy in a human subject by analyzing pseudo-projection images of cells obtained from a sputum specimen obtained from a subject employs a biological specimen classifier that identifies cells from the sputum specimen as normal or abnormal. If abnormal cells are detected, then the abnormal cells are further classified as dysplastic or cancerous. If the cells are classified as dysplastic, then an immunomodulating agent is administered to the subject over a predetermined time period designed to achieve a therapeutic dosage.

公开(公告)号：US20210049425A1

公开(公告)日：2021-02-18

申请号：US16976015

申请日：2019-02-28

申请人： VISIONGATE, INC.

发明人： Michael G. Meyer ,  Laimonis Kelbauskas ,  Rahul Katdare ,  Daniel J. Sussman ,  Timothy Bell ,  Alan C. Nelson

IPC分类号： G06K9/62 ,  G01N15/14 ,  G01N33/483 ,  G06T11/00 ,  G06K9/46 ,  G06T7/00

摘要： A method for a system and method for morphometric detection of malignancy associated change (MAC) is disclosed including the acts of obtaining a sample; imaging cells to produce 3D cell images for each cell; measuring a plurality of different structural biosignatures for each cell from its 3D cell image to produce feature data; analyzing the feature data by first using cancer case status as ground truth to supervise development of a classifier to test the degree to which the features discriminate between cells from normal or cancer patients; using the analyzed feature data to develop classifiers including, a first classifier to discriminate normal squamous cells from normal and cancer patients, a second classifier to discriminate normal macrophages from normal and cancer patients, and a third classifier to discriminate normal bronchial columnar cells from normal and cancer patients.

公开(公告)号：US20170003267A1

公开(公告)日：2017-01-05

申请号：US14788015

申请日：2015-06-30

申请人： VISIONGATE, INC.

发明人： Michael G. Meyer ,  Rahul Katdare ,  Chris Presley ,  Timothy Bell

IPC分类号： G01N33/483

CPC分类号： G01N33/4833 ,  G01N15/14 ,  G01N15/1434 ,  G01N15/147 ,  G01N15/1475 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1445 ,  G01N2015/1486 ,  G06T11/006 ,  G06T2207/20081

摘要： A cytological analysis test for 3D cell classification from a specimen. The method includes isolating and preserving a cell from the specimen and enriching the cell before embedding the enriched cell into an optical medium. The embedded cell is injected into a capillary tube where pressure is applied until the cell appears in a field of view of a pseudo-projection viewing subsystem to acquire a pseudo-projection image. The capillary tube rotates about a tube axis to provide a set of pseudo-projection images for each embedded cell which are reconstructed to produce a set of 3D cell reconstructions. Reference cells are classified and enumerated and a second cell classifier detects target cells. An adequacy classifier compares the number of reference cells against a threshold value of enumerated reference cells to determine specimen adequacy.

摘要翻译： 来自标本的3D细胞分类的细胞学分析测试。 该方法包括从样品中分离和保存细胞并在将富集的细胞嵌入光学介质之前富集细胞。 将嵌入式电池注入毛细管中，其中施加压力，直到细胞出现在伪投影观察子系统的视场中以获得伪投影图像。 毛细管围绕管轴旋转，以为每个嵌入式单元提供一组伪投影图像，这些图像被重建以产生一组3D细胞重建。 参考细胞被分类和枚举，第二细胞分类器检测靶细胞。 适当的分类器将参考单元的数量与枚举的参考单元的阈值进行比较，以确定样本充足度。

公开(公告)号：US08368035B2

公开(公告)日：2013-02-05

申请号：US13402149

申请日：2012-02-22

申请人： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

发明人： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

IPC分类号： G01N21/64

CPC分类号： G01N21/4795 ,  G01N15/1436 ,  G01N2015/1445 ,  G01N2021/1787 ,  G06T11/006

摘要： A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.

摘要翻译： 用于光学断层摄影系统中的细胞的3D成像的方法包括相对于显微镜物镜移动生物体以呈现不同的视角。 用具有限制在150nm和390nm之间的波长的光谱带宽的辐射照射该生物物体。 利用摄像机从多个不同的视角检测透过生物物体和显微镜物镜的辐射。 形成来自感测辐射的生物物体的多个伪投影，并重建多个伪投影以形成该细胞的3D图像。

公开(公告)号：US20100296713A1

公开(公告)日：2010-11-25

申请号：US12470413

申请日：2009-05-21

申请人： Michael G. Meyer ,  Rahul Katdare ,  David E. Steinhauer ,  J. Richard Rahn

发明人： Michael G. Meyer ,  Rahul Katdare ,  David E. Steinhauer ,  J. Richard Rahn

IPC分类号： G06K9/00 ,  G06T17/00

CPC分类号： G06K9/00147 ,  G06T7/0002 ,  G06T11/008 ,  G06T2200/04 ,  G06T2207/10101 ,  G06T2207/30024 ,  G06T2207/30168 ,  G06T2211/412

摘要： A system and method for detecting poor quality images in an optical tomography system includes an acquisition apparatus for acquiring a set of pseudo-projection images of an object having a center of mass, where each of the set of pseudo-projection images is acquired at a different angle of view. A reconstruction apparatus is coupled to receive the pseudo-projection images, for reconstruction of the pseudo-projection images into 3D reconstruction images. A quality apparatus is coupled to receive the 3D reconstruction images and operates to detect of selected features that characterize poor quality reconstructions.

摘要翻译： 一种用于在光学断层摄影系统中检测质量差的图像的系统和方法包括：获取装置，用于获取具有质心的物体的一组伪投影图像，其中在一组伪投影图像中获取每个伪投影图像 不同的视角。 耦合重建装置以接收伪投影图像，用于将伪投影图像重建成3D重建图像。 耦合质量装置以接收3D重建图像并操作以检测表征劣质重建的所选特征。

公开(公告)号：US20100214639A1

公开(公告)日：2010-08-26

申请号：US12391096

申请日：2009-02-23

申请人： Mathew D. Watson ,  Jon W. Hayenga

发明人： Mathew D. Watson ,  Jon W. Hayenga

IPC分类号： G02B21/06 ,  G02B26/10

CPC分类号： G02B21/025 ,  G01N21/4795 ,  G02B21/002

摘要： An object of interest is illuminated within the field of view of a microscope objective lens located to receive light passing through the object of interest. Light transmitted through the microscope objective lens impinges upon a variable power element. The variable power element is driven with respect to the microscope objective lens to scan through multiple focal planes in the object of interest. Light transmitted from the variable power element is sensed by a sensing element or array.

摘要翻译： 感兴趣的物体在位于接收通过感兴趣物体的光的显微镜物镜的视场内被照亮。 透过显微镜物镜的光照射在可变功率元件上。 可变功率元件相对于显微镜物镜被驱动以扫描感兴趣对象中的多个焦平面。 从可变功率元件发射的光由感测元件或阵列感测。

公开(公告)号：US20070071357A1

公开(公告)日：2007-03-29

申请号：US11558593

申请日：2006-11-10

申请人： John Rahn ,  Alan Nelson

发明人： John Rahn ,  Alan Nelson

IPC分类号： G06K9/40 ,  G06K9/00

CPC分类号： G02B21/26 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/05 ,  G01N21/4795 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2021/0346 ,  G01N2021/1787 ,  G01N2201/0813 ,  G01N2223/612 ,  G02B21/365 ,  G06T11/005 ,  G06T11/006 ,  G06T2211/412 ,  G06T2211/421

摘要： An apparatus and method for correction of relative object-detector motion between successive views during optical tomographic imaging in three dimensions. An object of interest is illuminated to produce an image. A lateral offset correction value is determined for the image. An axial offset correction value is determined for the image. The lateral offset correction value and the axial offset correction value are applied to the image to produce a corrected file image.

摘要翻译： 一种用于在三维尺度下在光学层析成像期间校正连续视图之间的相对物体检测器运动的装置和方法。 感兴趣的对象被照亮以产生图像。 确定图像的横向偏移校正值。 为图像确定轴向偏移校正值。 将横向偏移校正值和轴向偏移校正值应用于图像以产生校正的文件图像。

公开(公告)号：US20060096358A1

公开(公告)日：2006-05-11

申请号：US10975162

申请日：2004-10-28

申请人： Mark Fauver ,  Alan Nelson

发明人： Mark Fauver ,  Alan Nelson

IPC分类号： G01N30/00

CPC分类号： G02B21/0004 ,  B82Y10/00 ,  B82Y20/00 ,  G01N15/147 ,  G01N15/1475 ,  G01N2021/1787

摘要： A rotational system including a cylindrical container with a cylindrical container axis. The cylindrical container is inserted into at least one pair of opposing polymer grippers. A motor is coupled to rotate the cylindrical container.

摘要翻译： 一种旋转系统，包括具有圆柱形容器轴线的圆柱形容器。 将圆筒形容器插入至少一对相对的聚合物夹持器中。 电动机联接以旋转圆柱形容器。

公开(公告)号：US10890595B2

公开(公告)日：2021-01-12

申请号：US15782760

申请日：2017-10-12

申请人： VISIONGATE, INC.

发明人： Nathaniel Evans ,  Jon W. Hayenga

IPC分类号： G01N1/10 ,  G01N35/10 ,  B01L3/00 ,  G01N15/14 ,  G01N21/01 ,  G01N21/17 ,  G01N33/487

摘要： A method for pre-positioning a coaxial sample and sheath combination includes calculating a load shape profile including a plurality of layers of substantially equal volume. The calculated load shape profile is incrementally divided into cross-sectional slices at a first set of distance coordinates along a first axis. Each cross-sectional slice transects the plurality of layers. A sample includes a number of objects residing in solution. A sample chamber is loaded with the sample by incrementally dispensing the sample in a plurality of portions along a vertical axis divided into a second set of distance coordinates proportional to the first set of distance coordinates, where each portion has a volume proportional to the cross-sectional slice at the first distance coordinate nearest in value to the second distance coordinate.

公开(公告)号：US08947510B2

公开(公告)日：2015-02-03

申请号：US12999515

申请日：2009-06-08

申请人： Michael G. Meyer ,  J. Richard Rahn ,  Anna V. Tourovskaia ,  Julia Oi Yan Yu ,  Christy A Lancaster ,  Thomas Neumann ,  Mark E. Fauver

发明人： Michael G. Meyer ,  J. Richard Rahn ,  Anna V. Tourovskaia ,  Julia Oi Yan Yu ,  Christy A Lancaster ,  Thomas Neumann ,  Mark E. Fauver

IPC分类号： H04N13/02 ,  G06T11/00 ,  G01N15/14

CPC分类号： G06T11/006 ,  G01N15/1434 ,  G01N15/1475 ,  G01N21/49 ,  G01N21/6458 ,  G01N2015/1445

摘要： A method for 3D imaging of a biologic object (1) in an optical tomography system where a subcellular structure of a biological object (1) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object (1) is moved relatively to a microscope objective (62) to present varying angles of view and the labeled biological object (1) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object (1) and the microscope objective (62) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object (1) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object (1).

摘要翻译： 一种用于在其中通过引入至少一种纳米颗粒 - 生物标志物标记生物体（1）的亚细胞结构的光学层析成像系统中的生物物体（1）的3D成像的方法。 标记的生物体（1）相对于显微镜物镜（62）移动以呈现不同的视角，并且用波长在150nm和900nm之间的辐射照射标记的生物物体（1）。 用彩色照相机或一组至少四个单色照相机检测在至少一个波长带内通过标记的生物物体（1）和显微镜物镜（62）发射的辐射。 形成并重构来自感测辐射的生物体（1）的多个横截面图像，以便制成标记的生物体（1）的3D图像。