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    DUAL POLARITY ANALYSIS OF NUCLEIC ACIDS
    2.
    发明申请
    DUAL POLARITY ANALYSIS OF NUCLEIC ACIDS 审中-公开

    公开(公告)号：US20170362641A1

    公开(公告)日：2017-12-21

    申请号：US15689120

    申请日：2017-08-29

    申请人： Enzo Life Sciences, Inc.

    发明人： Elazar RABBANI ,  James J. Donegan

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6809 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q2565/525 ,  C12Q2565/501 ,  C12Q2531/143

    摘要： This invention provides methods for characterizing the amounts of nucleic acids, including plus/minus determinations, the use of different constructs, the use of a library and a reference library. Expression may also be compared in two or more samples using the methods of this invention. Also provided are heterophasic arrays comprising labeled positive copies of nucleic acids hybridized to the array and labeled negative copies of nucleic acids hybridized to the array, in which the labeled positive copies are separately quantifiable from the labeled negative copies.

    Assembly of high fidelity polynucleotides
    4.
    发明授权
    Assembly of high fidelity polynucleotides 有权
    装配高保真多核苷酸

    公开(公告)号：US09217144B2

    公开(公告)日：2015-12-22

    申请号：US13520383

    申请日：2011-01-06

    申请人： Joseph Jacobson ,  Larry Li-Yang Chu

    发明人： Joseph Jacobson ,  Larry Li-Yang Chu

    IPC分类号： C12Q1/68 ,  C12N15/10

    CPC分类号： B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00722 ,  C12N15/1031 ,  C12Q1/6811 ,  C12Q1/6837 ,  C12Q2565/525 ,  C12Q2525/301

    摘要： Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed. Assembly methods include cycles of annealing, stringent wash and extension of polynucleotides comprising a sequence region complementary to immobilized template oligonucleotides. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.

    摘要翻译： 方法和装置涉及高保真多核苷酸的合成和降低在固体支持物上核酸合成过程中产生的序列错误。 具体地，公开了载体结合的模板寡核苷酸的设计。 组装方法包括退火循环，严格洗涤和延伸包含与固定化模板寡核苷酸互补的序列区的多核苷酸。 由此产生的无错误的合成核酸可用于各种应用，包括合成生物燃料和增值药物产品。

    Methods and Compositions to Detect Nucleic Acids in a Biological Sample
    7.
    发明申请
    Methods and Compositions to Detect Nucleic Acids in a Biological Sample 有权
    检测生物样品中核酸的方法和组成

    公开(公告)号：US20120231551A1

    公开(公告)日：2012-09-13

    申请号：US12845640

    申请日：2010-07-28

    申请人： Michael M. BECKER ,  Mehrdad R. MAJLESSI

    发明人： Michael M. BECKER ,  Mehrdad R. MAJLESSI

    IPC分类号： G01N33/53 ,  G01N21/76 ,  C07H21/04

    CPC分类号： C12Q1/6837 ,  C12Q1/6834 ,  C12Q2565/525 ,  C12Q2565/501 ,  C12Q2525/301

    摘要： Kits, reaction mixtures and methods for separating a target nucleic acid from a sample by using at least one hairpin capture probe oligonucleotide that has the structure 5′-X.sub.n a′ b′ c′ Y.sub.n-3′, wherein X and Y each comprise nucleic acid sequences that can form a double stranded stem portion, one of X or Y is a capture sequence that is a first member of a specific binding pair and the other of X or Y is a terminal sequence of the hairpin capture probe, and a′ b′ c′ comprises a target-complementary sequence flanked by X and Y to thereby form a loop portion of the hairpin, thus forming a capture hybrid that is separated from other sample components before the target nucleic acid is released from the capture support and hybridized to a detection probe that hybridizes specifically to the same sequence that is at least partially hybridized by the a′ b′ c′ portion of the capture probe, thus forming a detectable detection hybrid to indicate the presence of the target nucleic acid in the sample.

    摘要翻译： 试剂盒，反应混合物和通过使用至少一种具有结构5'-XbaI'b'c'Y-3'的发夹捕获探针寡核苷酸从样品中分离靶核酸的方法， 其中X和Y各自包含可以形成双链茎部分的核酸序列，X或Y之一是作为特异性结合对的第一个成员的捕获序列，X或Y中的另一个是末端序列 发夹捕获探针，并且'b'c'包含侧翼为X和Y的靶互补序列，从而形成发夹的环部分，从而形成捕获杂交体，其在靶核酸为靶标之前与其它样品组分分离 从捕获载体释放并与检测探针杂交，所述检测探针特异性与与捕获探针的'b'c'部分至少部分杂交的相同序列杂交，从而形成可检测的检测杂交体以指示存在 柏油 得到样品中的核酸。

    Methods and Compositions to Detect Nucleic Acids in a Biological Sample
    8.
    发明申请
    Methods and Compositions to Detect Nucleic Acids in a Biological Sample 有权
    检测生物样品中核酸的方法和组成

    公开(公告)号：US20110250599A1

    公开(公告)日：2011-10-13

    申请号：US13087282

    申请日：2011-04-14

    申请人： Michael M. BECKER ,  Mehrdad R. MAJLESSI

    发明人： Michael M. BECKER ,  Mehrdad R. MAJLESSI

    IPC分类号： C12Q1/68 ,  G01N33/569 ,  C07H21/00 ,  G01N33/53

    CPC分类号： C12Q1/6837 ,  C12Q1/6834 ,  C12Q2565/525 ,  C12Q2565/501 ,  C12Q2525/301

    摘要： Methods of the invention separate a target nucleic acid from a sample by using at least one capture probe oligonucleotide that contains a target-complementary region and a member of a specific binding pair that attaches the target nucleic acid to an immobilized probe on a capture support, thus forming a capture hybrid that is separated from other sample components before the target nucleic acid is released from the capture support and hybridized to a detection probe to form a detection hybrid that produces a detectable signal that indicates the presence of the target nucleic acid in the sample. Compositions for practicing the methods of the invention include a capture probe oligonucleotide made up a target-complementary region sequence and a covalently linked capture region sequence that includes a member of a specific binding pair.

    摘要翻译： 本发明的方法通过使用至少一种包含靶互补区的捕获探针寡核苷酸和将靶核酸附着在捕获载体上的固定化探针上的特异性结合对的成员来分离目标核酸， 从而形成在靶核酸从捕获载体释放之前与其它样品组分分离的捕获杂交物，并与检测探针杂交以形成检测杂交体，其产生可检测信号，其指示靶核酸存在于 样品。 用于实施本发明方法的组合物包括构成靶互补区序列的捕获探针寡核苷酸和包含特异性结合对成员的共价连接的捕获区序列。

    Reactions on a solid surface
    9.
    发明授权
    Reactions on a solid surface 有权
    固体表面上的反应

    公开(公告)号：US07820387B2

    公开(公告)日：2010-10-26

    申请号：US11926120

    申请日：2007-10-28

    申请人： Bruce P. Neri ,  Jeff G. Hall ,  Victor Lyamichev ,  Lloyd M. Smith

    发明人： Bruce P. Neri ,  Jeff G. Hall ,  Victor Lyamichev ,  Lloyd M. Smith

    IPC分类号： C12Q1/68

    CPC分类号： C12N9/22 ,  C12Q1/6823 ,  C12Q1/6827 ,  Y10S435/81 ,  Y10S435/822 ,  C12Q2565/525 ,  C12Q2561/109 ,  C12Q2525/301

    摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

    摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在固体支持物上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。