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公开(公告)号：US09962623B2
公开(公告)日：2018-05-08
申请号：US14363360
申请日：2012-12-06
摘要： The present invention relates to processes to remove butanol and other product alcohols from a fermentation broth employing vacuum vaporization.
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92.发明授权公开(公告)号：US09765365B2
公开(公告)日：2017-09-19
申请号：US14991512
申请日：2016-01-08
IPC分类号： C12P7/16 , C12N1/20 , C12N1/00 , C12N1/12 , C07H21/04 , C12N9/04 , C12N9/10 , C12N9/88 , C12N15/52 , C12P7/18 , C12P7/26 , C12P7/64
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1022 , C12N9/88 , C12N15/52 , C12P7/18 , C12P7/26 , C12P7/649 , C12Y101/01001 , C12Y101/01002 , C12Y101/01086 , C12Y101/01265 , C12Y202/01006 , C12Y401/01001 , C12Y401/01072 , C12Y402/01009 , Y02E50/10 , Y02E50/13
摘要： The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells.
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公开(公告)号：US09670511B2
公开(公告)日：2017-06-06
申请号：US13874737
申请日：2013-05-01
IPC分类号： C12M1/00 , C12M3/00 , C12P7/16 , C12N1/14 , C12N15/00 , B01D3/00 , B01D21/26 , C07C29/86 , C11B1/02 , C11B13/00 , C12P7/64 , C12P7/18
CPC分类号： C12P7/16 , A23K10/38 , B01D3/002 , B01D11/0492 , B01D21/26 , B01D21/262 , C07C29/86 , C11B1/025 , C11B13/00 , C12F3/10 , C12M1/00 , C12M21/12 , C12M45/04 , C12M45/09 , C12N1/14 , C12N15/00 , C12P7/18 , C12P7/6418 , Y02E50/10 , Y02E50/16 , Y02E50/17 , Y02W30/74
摘要： A method and system for efficiently producing a fermentative product alcohol such as butanol utilizing in situ product extraction are provided. The efficiency is obtained through separating undissolved solids after liquefying a given feedstock to create a feedstock and prior to fermentation, for example, through centrifugation. Removal of the undissolved solids avoids problems associated with having the undissolved solids present during in situ production extraction, and thereby increases the efficiency of the alcohol production.
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公开(公告)号：US09605281B2
公开(公告)日：2017-03-28
申请号：US13828353
申请日：2013-03-14
发明人： Stephane Francois Bazzana , James Timothy Cronin , Michael Charles Grady , David J. Lowe , Brian Michael Roesch , Joseph J. Zaher , Raymond Richard Zolandz
CPC分类号： C12P7/16 , C11B1/025 , C11B1/10 , C12M21/12 , C12M33/00 , C12M41/26 , C12M41/48 , C12M43/02 , C12M47/10 , C12P7/10 , C12P7/14 , C12P2203/00 , Y02E50/16
摘要： The present invention relates to the fermentative production of alcohols including ethanol and butanol, and processes for improving alcohol fermentation employing in situ product removal methods.
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公开(公告)号：US09550999B2
公开(公告)日：2017-01-24
申请号：US14335734
申请日：2014-07-18
IPC分类号： C12N1/20 , C12N1/00 , C12N9/00 , C12N15/00 , C12P7/16 , C07H21/04 , C12N15/81 , C12N9/02 , C12N9/10 , C12N9/88 , C12N15/52 , C12N15/74 , C12P7/18 , C12P7/26 , C12P7/46 , C12P7/56 , C12P13/06 , C12P13/08
CPC分类号： C12P7/16 , C12N9/0004 , C12N9/0008 , C12N9/1029 , C12N9/88 , C12N15/52 , C12N15/74 , C12N15/81 , C12P7/18 , C12P7/26 , C12P7/46 , C12P7/56 , C12P13/06 , C12P13/08 , C12Y102/01003 , C12Y102/07001 , C12Y114/15003 , C12Y118/01001 , C12Y118/01002 , C12Y203/01008 , C12Y203/01054 , C12Y401/01001 , C12Y401/02009 , Y02E50/10 , Y02P20/52
摘要： The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
摘要翻译： 本发明涉及重组宿主细胞，其包含：（i）编码将丙酮酸转化为乙醛，乙酰磷酸或乙酰辅酶A的多肽的内源基因中的至少一个缺失，突变和/或取代; 和（ii）编码具有磷酸酮醇酶活性的多肽的异源多核苷酸。 本发明还涉及重组宿主细胞，其还包含（iii）编码具有磷酸转乙酰酶活性的多肽的异源多核苷酸。
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公开(公告)号：US09481842B2
公开(公告)日：2016-11-01
申请号：US13624669
申请日：2012-09-21
IPC分类号： C10L1/182 , C10L1/16 , C10G3/00 , C10G35/04 , C10G63/00 , C10G65/04 , C10G65/08 , C10G69/04 , C10G69/08 , C10G11/18 , C10G45/02 , C10G45/44 , C10G45/58 , C10L1/02
CPC分类号： C10L1/1824 , C10G3/00 , C10G11/18 , C10G35/04 , C10G45/02 , C10G45/44 , C10G45/58 , C10G63/00 , C10G65/043 , C10G65/08 , C10G69/04 , C10G69/08 , C10G2300/305 , C10G2300/4043 , C10G2300/80 , C10G2400/02 , C10G2400/04 , C10L1/023 , C10L1/1616 , C10L10/02 , C10L2200/0415 , C10L2200/0423 , C10L2230/22 , C10L2270/023 , C10L2290/24 , Y02P30/20
摘要： Systems and processes for the production of fuel and fuel blends involve the production of fuels for blending with one or more alcohols such as ethanol and/or butanol. A method for producing a fuel blend includes blending a light distillate product from an oil refinery with butanol. The fuel blending can be at the oil refinery.
摘要翻译： 用于生产燃料和燃料混合物的系统和方法涉及生产用于与一种或多种醇如乙醇和/或丁醇共混的燃料。 一种生产燃料混合物的方法包括将来自炼油厂的轻馏分产物与丁醇混合。 燃油混合可以在炼油厂。
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公开(公告)号：US09422582B2
公开(公告)日：2016-08-23
申请号：US13840676
申请日：2013-03-15
发明人： Larry Cameron Anthony , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica McElvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
CPC分类号： C12P7/16 , C12N1/38 , C12N9/0006 , C12N15/81 , C12N15/815 , C12P7/42 , Y02E50/10 , Y02P20/52
摘要： Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
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公开(公告)号：US09422581B2
公开(公告)日：2016-08-23
申请号：US13840649
申请日：2013-03-15
发明人： Larry Cameron Anthony , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica McElvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
CPC分类号： C12P7/16 , C12N1/38 , C12N9/0006 , C12N15/81 , C12N15/815 , C12P7/42 , Y02E50/10 , Y02P20/52
摘要： Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
摘要翻译： 本文提供重组酵母宿主细胞及其用于生产异丁醇的方法。 提供的酵母宿主细胞包含异丁醇生物合成途径和降低或消除的醛脱氢酶活性，降低或消除的乙酰乳酸还原酶活性中的至少一种; 或编码具有酮酸酸还原异构酶活性的多肽的异源多核苷酸。
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公开(公告)号：US09371547B2
公开(公告)日：2016-06-21
申请号：US14481950
申请日：2014-09-10
IPC分类号： C12P7/16 , C12N1/14 , C12N15/00 , B01D3/00 , B01D21/26 , C07C29/86 , C11B1/02 , C11B13/00 , C12M1/00 , C12P7/64 , C12P7/18
CPC分类号： C12P7/16 , A23K10/38 , B01D3/002 , B01D11/0492 , B01D21/26 , B01D21/262 , C07C29/86 , C11B1/025 , C11B13/00 , C12F3/10 , C12M1/00 , C12M21/12 , C12M45/04 , C12M45/09 , C12N1/14 , C12N15/00 , C12P7/18 , C12P7/6418 , Y02E50/10 , Y02E50/16 , Y02E50/17 , Y02W30/74
摘要： In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant. The fermentation product extractant can be contacted with a fermentation broth for in situ removal of a product alcohol.
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公开(公告)号：US09297028B2
公开(公告)日：2016-03-29
申请号：US11527995
申请日：2006-09-27
申请人： Gail K. Donaldson , Lixuan Lisa Huang , Lori Ann Maggio-Hall , Vasantha Nagarajan , Charles E. Nakamura , Wonchul Suh
发明人： Gail K. Donaldson , Lixuan Lisa Huang , Lori Ann Maggio-Hall , Vasantha Nagarajan , Charles E. Nakamura , Wonchul Suh
IPC分类号： C12P7/16 , C12N9/10 , C12N9/04 , C12N9/88 , C12N9/02 , C12N9/06 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P7/16 , C12N9/0004 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1029 , C12N9/88 , C12Y101/01157 , C12Y103/99002 , C12Y203/01009 , C12Y402/01055 , Y02E50/10 , Y02P20/52
摘要： Methods for the fermentive production of four carbon alcohols is provided. Specifically, butanol, preferably 1-butanol is produced by the fermentive growth of a recombinant bacterium expressing a 1-butanol biosynthetic pathway.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达1-丁醇生物合成途径的重组细菌的发酵生长来制备丁醇，优选1-丁醇。
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