摘要:
Succinic acid is produced by allowing a bacterium modified to enhance fumarate reductase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. More preferably, succinic acid is produced by allowing a bacterium modified to enhance activities of fumarate reductase and pyruvate carboxylase and decrease lactate dehydrogenase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. Succinic acid is obtained by collecting the produced succinic acid.
摘要:
A method of producing coryneform bacteria having an improved amino acid- or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid d- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method can construct a mutant capable of suitably enriching or controling the expression of an intended gene without using a plasmid and also capable of producing amino acids in a high yield, by the recombination or mutation.
摘要:
A method of producing coryneform bacteria having improved amino acid or nucleic acid productivity comprising the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence, or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and selecting a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows the construction of a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield by recombination or mutation.
摘要:
L-arginine or L-lysine is produced by culturing a coryneform bacterium having an L-arginine- or L-lysine-producing ability and modified so that glutamine synthetase activity is enhanced, e.g., a coryneform bacterium which is modified so that adenylylation of glutamine synthetase is eliminated. L-arginine or L-lysine are produced by culturing the bacterium in a medium and allowing L-arginine or L-lysine to accumulate in the medium, and collecting the L-arginine or L-lysine from the medium.
摘要:
A stencil is made by thermally forming perforations arranged in both a main scanning direction and a sub-scanning direction in a thermoplastic resin film of heat-sensitive stencil material by the use of a heat source which is heated through supply of energy. Supply of energy to the heat source is cut when a time interval not shorter than 50% and not longer than 100% of an estimated perforating time lapses from the time at which supply of energy to the heat source is started. The estimated perforating time is a time interval expected to be necessary for a perforation to be produced by the heat of the heat source and to be enlarged to a desired size as a final size as measured from the time at which supply of energy to the heat source is started.
摘要:
A method of pipe line washing is described, in which raw pulp liquid is washed by clean water or washing liquid having a lower concentration than liquid chemicals in the raw pulp liquid by making use of all or a part of a pipe line.
摘要:
Provided are an electric storage device in which a current collector is not easily broken even when vibration is applied, and a vehicle having this electric storage device. This electric storage device includes a case having a first inner surface and a second inner surface adjacent to the first inner surface, an electrode assembly housed in the case and including a positive electrode plate and a negative electrode plate insulated from each other, an electrode terminal disposed outside the case, and a current collector housed in the case and electrically connecting the electrode assembly and the electrode terminal to each other. A distal end edge of a distal end portion of the current collector is supported on the first inner surface of the case.
摘要:
A current collecting member includes first portion second portions. The second portion includes a base portion the first end portion of which is connected to the second end portion of the first portion, a twisted portion the first end portion of which is connected to the second end portion of the base portion, and an insertion portion the first end portion of which is connected to the second end portion of the twisted portion and which is inserted into a winding center of the electrode assembly, so as to be electrically connected to the electrode assembly.
摘要:
Provided is an electric storage device that can ensure seal in a peripheral area of a portion in a defining wall of a case through which a rivet passes even when a rivet is inserted through the defining wall of the case and the rivet is caulked. The electric storage device includes: an electrode assembly; a case that houses the electrode assembly, the case including a defining wall; and a rivet, wherein a peripheral area of a portion in the defining wall through which the rivet passes has a higher hardness than the remaining area.
摘要:
A fluid transport device transports fluid containing electrolytes within a flow channel. At least a portion of the inner walls of the flow channels are hydrophilic from the inlet to the outlet thereof except at at least one valve portion. The device also includes: the valve portions, which are hydrophobic and function to block transport of at least one fluid; electrodes, which are provided at the at least one valve portion and function to reduce the surface tension of the fluid; and air vents, which are provided at the at least one valve portion and function to introduce air in order to block the fluid.