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91.发明授权N8 -and c8 -linked purine based and structurally related heterocycles as universal nucleosides used oligonucleotides hybridization 有权N8-和c8连接的基于嘌呤和结构相关的杂环作为通用核苷使用寡核苷酸杂交公开(公告)号：US07063945B2
公开(公告)日：2006-06-20
申请号：US10221306
申请日：2001-03-27
摘要： The present invention is directed to a nucleic acid binding compound comprising N8- or C8-linked purine bases or structurally related heterocycles, a compound useful for the preparation of such compound, a binding product of this nucleic acid binding compound with a nucleic acid, a method for the determination of a nucleic acid using said compound, and several uses of 8-linked purine bases and structurally related heterocycles. Compounds according to the present invention have advantageous properties when used in hybridization methods.
摘要翻译： 本发明涉及包含N 8 - 或C 8 - 连接的嘌呤碱基或结构相关杂环的核酸结合化合物，可用于制备该化合物的化合物 ，该核酸结合化合物与核酸的结合产物，使用所述化合物测定核酸的方法，以及8-连接的嘌呤碱基和结构相关杂环的若干用途。 当用于杂交方法时，根据本发明的化合物具有有利的性质。
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92.发明授权Method for improving the stability of linear DNA in cell-free in vitro transcription/translation systems 失效提高无细胞体外转录/翻译系统中线性DNA稳定性的方法公开(公告)号：US07011958B2
公开(公告)日：2006-03-14
申请号：US10124663
申请日：2002-04-17
申请人： Manfred Watzele , Thomas Hoffmann , Cordula Nemetz , Dieter Heindl , Thomas Metzler , Wolfgang Mutter
发明人： Manfred Watzele , Thomas Hoffmann , Cordula Nemetz , Dieter Heindl , Thomas Metzler , Wolfgang Mutter
摘要： The present invention concerns a method for improving the stability of linear short DNA towards exonucleases in cell-free in vitro transcription/translation systems using lysates containing exonucleases or in cellular systems containing exonucleases, wherein the stability of the linear short DNA is improved by adding unspecific linear DNA.
摘要翻译： 本发明涉及使用含有外切核酸酶的裂解物或含有核酸外切酶的细胞系统，在无细胞体外转录/翻译系统中提高线性短DNA对外源核酸酶的稳定性的方法，其中线性短DNA的稳定性通过添加非特异性 线性DNA。
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公开(公告)号：US20050142598A1
公开(公告)日：2005-06-30
申请号：US11001785
申请日：2004-12-02
申请人： Dieter Heindl , Astrid Reiser , Karim Tabiti , Martina Junius , Alexander Hauber
发明人： Dieter Heindl , Astrid Reiser , Karim Tabiti , Martina Junius , Alexander Hauber
CPC分类号： C07H21/00 , Y10S436/808
摘要： The invention is directed to an oligonucleotide comprising a molecular rod, wherein preferentially the molecular rod covalently connects the 3′ end of a first nucleotide residue with the 5′ end of a second nucleotide residue. Such oligonucleotides are highly advantageous for real time PCR melting curve analysis.
摘要翻译： 本发明涉及包含分子棒的寡核苷酸，其中优先分子棒共价连接第一个核苷酸残基的3'末端与第二个核苷酸残基的5'末端。 这样的寡核苷酸对于实时PCR融合曲线分析是非常有利的。
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公开(公告)号：US20050037410A1
公开(公告)日：2005-02-17
申请号：US10903992
申请日：2004-07-30
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
CPC分类号： C12Q1/6818 , C12Q2561/113 , C12Q2531/113 , C12Q2521/319
摘要： The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3′-5′ exonuclease having a temperature optimum above 37° C., a pair of amplification primers, deoxynucleoside triphosphates, a detecting oligonucleotide carrying a first label and a second label, said first label being capable of acting as a fluorescent reporter entity when excited with light of an appropriate wavelength, said second label being capable of acting as a fluorescence quenching entity of said fluorescent reporter entity, characterized in that one label is bound to the 3′ end of said detecting oligonucleotide, and further characterized in that the other label is bound either internally or at the 5′ end of said detecting oligonucleotide, and monitoring fluorescence of said fluorescent reporter entity at least after a plurality of amplification cycles.
摘要翻译： 本发明涉及用于扩增和检测靶核酸的方法和组合物，其包括在热稳定性DNA聚合酶，耐热双链依赖性3'-5'的存在下使所述靶核酸进行实时PCR扩增反应， 温度最高在37℃以上的外切核酸酶，一对扩增引物，脱氧核苷三磷酸，携带第一标记和第二标记的检测寡核苷酸，所述第一标记当用 所述第二标记能够用作所述荧光报道实体的荧光猝灭实体，其特征在于一个标记与所述检测寡核苷酸的3'末端结合，其特征还在于另一个标记在内部结合 或在所述检测寡核苷酸的5'末端，并监测所述荧光报告的荧光 至少在多个放大循环之后。
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公开(公告)号：US20050004078A1
公开(公告)日：2005-01-06
申请号：US10816298
申请日：2004-04-01
IPC分类号： G01N33/53 , A61K31/513 , A61K31/52 , A61K31/675 , C07D405/04 , C07D495/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H21/00 , C07H21/04 , C12P19/34 , C12P19/38 , C12Q1/68 , G01N33/566 , G01N33/58 , G01N37/00
CPC分类号： A61K31/513 , A61K31/52 , A61K31/675 , C07D405/04 , C07D495/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H21/00 , C07H21/04 , C12P19/34 , C12P19/38 , C12Q1/68 , G01N33/53 , G01N33/566 , G01N33/58 , G01N37/00 , Y02P20/55
摘要： The present invention is related to compounds comprising six-membered rings capable of being incorporated into nucleic acids. Particularly, the six-membered ring is a derivative of cyclohexane, cyclohexene, tetrahydropyran, tetrahydrothiopyran or piperidine. These compounds may be used to build up oligomeric compounds. The invention is further related to uses of these oligomeric compounds for hybridization and as probes. In addition, methods for the detection of nucleic acids are provided wherein the oligomeric compounds are used.
摘要翻译： 本发明涉及能够并入核酸中的包含六元环的化合物。 特别地，六元环是环己烷，环己烯，四氢吡喃，四氢噻喃或哌啶的衍生物。 这些化合物可用于构建低聚化合物。 本发明还涉及这些寡聚化合物用于杂交和作为探针的用途。 此外，提供了检测核酸的方法，其中使用低聚化合物。
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公开(公告)号：US09896666B2
公开(公告)日：2018-02-20
申请号：US12859654
申请日：2010-08-19
CPC分类号： C12N9/0006 , C12N9/96
摘要： The present invention relates to a method for stabilizing an enzyme by storing the enzyme in the presence of a stable coenzyme. The present invention further relates to an enzyme stabilized with a stable coenzyme, and to the use thereof in test elements for detecting analytes.
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公开(公告)号：US09347092B2
公开(公告)日：2016-05-24
申请号：US12705679
申请日：2010-02-15
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2523/319 , C12Q2525/197 , C12Q2563/149 , C12Q2565/537
摘要： The present invention provides a solid support which is preferably a bead comprising at least two sequence specific amplification primers wherein at least one primer is bound to the support with an inducible cleavable linker. The present invention also provides various method for preparing a solid support comprising at least two sequence specific primers, further characterized in that at least one of the primers is cleavable.
摘要翻译： 本发明提供了一种固体支持物，其优选是包含至少两个序列特异性扩增引物的珠，其中至少一个引物用可诱导的可切割连接子与载体结合。 本发明还提供了用于制备包含至少两个序列特异性引物的固体支持物的各种方法，其进一步的特征在于至少一种引物是可切割的。
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98.发明授权Method and substances for the preparation of N-substituted pyridinium compounds 有权用于制备N-取代的吡啶鎓化合物的方法和物质公开(公告)号：US09085535B2
公开(公告)日：2015-07-21
申请号：US13359661
申请日：2012-01-27
申请人： Dieter Heindl , Peter Gebauer
发明人： Dieter Heindl , Peter Gebauer
IPC分类号： C07D213/803 , C07D213/80
CPC分类号： C07D213/803 , C07D213/80
摘要： Methods for the synthesis of N-substituted carboxylated pyridinium compounds by reaction of a pentamethine precursor with a primary amine are provided. In this reaction an N-substituted alkoxycarbonyl pyridinium heterocycle is formed.
摘要翻译： 提供了通过五胺前体与伯胺反应合成N-取代的羧化吡啶鎓化合物的方法。 在该反应中，形成N-取代的烷氧基羰基吡啶鎓杂环。
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99.发明申请公开(公告)号：US20130303768A1
公开(公告)日：2013-11-14
申请号：US13945556
申请日：2013-07-18
申请人： Peter Gebauer , Dieter Heindl , Carina Horn
发明人： Peter Gebauer , Dieter Heindl , Carina Horn
IPC分类号： C07F9/58 , C07C251/12
CPC分类号： C07F9/58 , C07C251/12 , C07D213/46
摘要： A method for the synthesis of N-substituted 3-acylated pyridinium compounds by reacting a pentamethine precursor with a primary amine.
摘要翻译： 通过使五甲胺前体与伯胺反应合成N-取代的3-酰化吡啶鎓化合物的方法。
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公开(公告)号：US20120276565A1
公开(公告)日：2012-11-01
申请号：US13400209
申请日：2012-02-20
申请人： Wolfgang Roedel , Carina Horn , Nelli Steinke , Nadine Bucci , Thomas Meier , Rainer Schmuck , Rolf Nagel , Dieter Heindl
发明人： Wolfgang Roedel , Carina Horn , Nelli Steinke , Nadine Bucci , Thomas Meier , Rainer Schmuck , Rolf Nagel , Dieter Heindl
CPC分类号： C12N9/96 , C12N9/0006 , C12N9/0036 , C12Q1/26 , C12Q1/32 , C12Y101/01001 , C12Y101/01002 , C12Y101/01047 , C12Y101/01049 , C12Y106/05002 , C12Y106/99
摘要： Methods for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme are disclosed. In addition, an enzyme stabilized with a stabilized coenzyme as well as the use thereof in test elements for detecting analytes are also disclosed. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.
摘要翻译： 公开了通过在稳定的辅酶的存在下储存酶来稳定酶的方法。 此外，还公开了用稳定的辅酶稳定的酶以及其用于检测分析物的测试元件中的用途。 其他方面包括独特的组合物，方法，技术，涉及酶稳定化的系统和装置。
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