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794 条记录 (耗时 0.052 秒)

    Sequence-specific detection of methylation in biomolecules
    103.
    发明授权
    Sequence-specific detection of methylation in biomolecules 有权
    生物分子中甲基化的序列特异性检测

    公开(公告)号：US08129106B2

    公开(公告)日：2012-03-06

    申请号：US11570672

    申请日：2005-06-14

    申请人： Elmar Weinhold ,  Thomas Meier ,  Hartmut Düfel ,  Christine Markert-Hahn ,  Rainer Schmuck

    发明人： Elmar Weinhold ,  Thomas Meier ,  Hartmut Düfel ,  Christine Markert-Hahn ,  Rainer Schmuck

    IPC分类号： C12Q1/68 ,  C12Q1/70 ,  G01N33/53 ,  C07H15/00 ,  C07H21/04 ,  C07H19/04

    CPC分类号： C07H19/16 ,  C12Q1/6827 ,  C12Q2537/164 ,  C12Q2522/101 ,  C12Q2521/125

    摘要： A method for detecting sequence specific methylation in a biomolecule, comprising: (a) contacting the biomolecule with an S-adenosyl-L-methionine-dependent methyltransferase in the presence of a detectable cofactor of said methyltransferase; and (b) detecting whether the recognition sequence of said methyltransferase has been modified with the cofactor or a derivative thereof, wherein modification of the recognition sequence of said methyltransferase is indicative of an absence of methylation at said recognition sequence. Also disclosed is a cofactor specific for S-adenosyl-L-methionine-dependent methyltransferases, wherein said cofactor is an N-adenosylaziridine derivative with a reporter group attached to the 6 or 7 position of the adenine ring or attached to the aziridine ring. A complex of the cofactor and a methyltransferase a composition comprising the cofactor or the complex and the use of the cofactor or the complex for detecting sequence-specific methylation in DNA molecules are also disclosed.

    摘要翻译： 一种用于检测生物分子中序列特异性甲基化的方法，包括：（a）在所述甲基转移酶的可检测辅因子的存在下，使所述生物分子与S-腺苷-L-甲硫氨酸依赖性甲基转移酶接触; 和（b）检测所述甲基转移酶的识别序列是否已经用辅因子或其衍生物修饰，其中所述甲基转移酶的识别序列的修饰指示在所述识别序列处不存在甲基化。 还公开了对S-腺苷-L-甲硫氨酸依赖性甲基转移酶特异性的辅因子，其中所述辅因子是N-腺苷氮丙啶衍生物，其报告基团连接到腺嘌呤环的6或7位或连接到氮丙啶环上。 还公开了辅因子和甲基转移酶的复合物，其包含辅因子或复合物以及辅因子或复合物用于检测DNA分子中的序列特异性甲基化的组合物。

    Biomass energy recovery apparatus
    105.
    发明授权
    Biomass energy recovery apparatus 有权
    生物质能回收装置

    公开(公告)号：US08117974B2

    公开(公告)日：2012-02-21

    申请号：US12173251

    申请日：2008-07-15

    申请人： Raymond C Ganga ,  Greg Imig ,  Blake McBurney ,  Robert Jensen ,  John Kerr ,  Steve J Reust

    发明人： Raymond C Ganga ,  Greg Imig ,  Blake McBurney ,  Robert Jensen ,  John Kerr ,  Steve J Reust

    IPC分类号： F23D1/00 ,  F23J15/00

    CPC分类号： F23G7/10 ,  F23C6/045 ,  F23C2900/06041 ,  F23G5/24 ,  F23G5/46 ,  F23G5/50 ,  F23G2207/105 ,  F23G2207/60 ,  F23G2900/00001 ,  F23J1/00 ,  F23J7/00 ,  F23J15/006 ,  F23J2215/101 ,  F23J2219/10 ,  F23J2219/80

    摘要： An apparatus for extracting energy from fiber introduced as a fuel into a vertically elongated combustion chamber having a suspension burner capable of projecting a flame down the axis of the combustion chamber with a heat collection surface located radially from the flame and below the burner, and an exhaust opening located below the flame and below at least a portion of the heat collection surface, in which the combusted fiber yields a mixture containing hot flue gas and molten ash above the exhaust opening and heat transfers therefrom to the heat collection surface prior to any substantial contact of ash to a surface of the combustion chamber, to yield a mixture containing warm flue gas and non-molten ash, that is thereafter cooled to yield a mixture containing cool flue gas and non-molten ash.

    摘要翻译： 一种用于从作为燃料引入的纤维中提取能量的装置，其具有能够沿着燃烧器的轴线位于燃烧室的轴线处的火焰下方的集热表面而将火焰向下突出的悬架燃烧器， 排气开口位于火焰下方并且在集热表面的至少一部分下方，其中燃烧的纤维产生含有热烟道气和在排气口上方的熔融灰分的混合物，并且在任何实质的 将灰分与燃烧室的表面接触，得到含有热烟气和非熔融灰的混合物，然后冷却，得到含有冷烟道气和非熔融灰的混合物。

    Benzanilides with insecticidal activity
    106.
    发明授权
    Benzanilides with insecticidal activity 失效
    具有杀虫活性的苯甲酰苯胺

    公开(公告)号：US08110689B2

    公开(公告)日：2012-02-07

    申请号：US11917411

    申请日：2006-06-02

    申请人： Katsuaki Wada ,  Tetsuya Murata ,  Katsuhiko Shibuya ,  Eiichi Shimojo

    发明人： Katsuaki Wada ,  Tetsuya Murata ,  Katsuhiko Shibuya ,  Eiichi Shimojo

    IPC分类号： C07D213/00 ,  C07C233/00 ,  A61K31/44 ,  A61K31/16

    CPC分类号： C07C323/42 ,  A01N37/30 ,  A01N41/10 ,  A01N43/40 ,  C07C205/11 ,  C07C205/26 ,  C07C211/52 ,  C07C317/28 ,  C07C317/50 ,  C07C323/62 ,  C07D209/48 ,  C07D213/40 ,  C07D213/75

    摘要： Benzanilides of the formula: in which X represents hydrogen, halogen, nitro, C1-6 alkylthio, C1-6 alkylsulfinyl, C1-6 alkylsulfonyl or C1-6 alkylsulfonyloxy; Y represents halogen or C1-6 alkyl; R1 represents C1-6 alkyl, C1-6 alkylthio-C1-6 alkyl, C1-6 alkylsulfinyl-C1-6 alkyl or C1-6 alkylsulfonyl-C1-6 alkyl; R2 represents hydrogen, C1-6 alkyl or C1-6 haloalkyl; R3 represents hydrogen or hydroxy; W represents CH or N; and Q represents optionally substituted phenyl or optionally substituted pyridyl wherein the substituent is at least one group selected from the group consisting of halogen, C1-6 haloalkyl, C1-6 haloalkoxy, C1-6 haloalkylthio, C1-6 haloalkylsulfinyl and C1-6 haloalkylsulfoxy; provided that when R3 is hydroxy, R2 is not C1-6 alkyl, or when R2 is C1-6 haloalkyl, R3 is hydroxy, W is CH, and the substituents of Q are two or more C1-6 haloalkyl. Insecticides comprising the benzanilides are herein provided.

    摘要翻译： 下式的苯甲酰苯胺：其中X表示氢，卤素，硝基，C 1-6烷硫基，C 1-6烷基亚磺酰基，C 1-6烷基磺酰基或C 1-6烷基磺酰氧基; Y表示卤素或C 1-6烷基; R 1表示C 1-6烷基，C 1-6烷硫基-C 1-6烷基，C 1-6烷基亚磺酰基-C 1-6烷基或C 1-6烷基磺酰基-C 1-6烷基; R 2表示氢，C 1-6烷基或C 1-6卤代烷基; R3表示氢或羟基; W表示CH或N; 并且Q表示任选取代的苯基或任选取代的吡啶基，其中取代基是选自卤素，C 1-6卤代烷基，C 1-6卤代烷氧基，C 1-6卤代烷硫基，C 1-6卤代烷基亚磺酰基和C 1-6卤代烷基亚磺酰基中的至少一个基团 ; 条件是当R 3是羟基时，R 2不是C 1-6烷基，或者当R 2是C 1-6卤代烷基时，R 3是羟基，W是CH，并且Q的取代基是两个以上的C 1-6卤代烷基。 本文提供了包含苯甲酰苯胺的杀虫剂。

    Methods and compositions for controlling plant pests
    110.
    发明授权
    Methods and compositions for controlling plant pests 有权
    防治植物害虫的方法和组成

    公开(公告)号：US08076533B2

    公开(公告)日：2011-12-13

    申请号：US13027816

    申请日：2011-02-15

    申请人： Kimberly S. Sampson ,  Daniel John Tomso

    发明人： Kimberly S. Sampson ,  Daniel John Tomso

    IPC分类号： C12N15/82 ,  C12N1/20 ,  C12N5/00 ,  C12P21/06 ,  A01H5/00

    CPC分类号： C07K14/325 ,  A01N37/46 ,  C07K14/32 ,  C12N15/8286 ,  Y02A40/162

    摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-24 or the nucleotide sequence set forth in SEQ ID NO:1-12 and 25-44, as well as variants and fragments thereof.

    摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了包含编码SEQ ID NO：13-24所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-12和25-44所示的核苷酸序列的分离的核酸分子，以及 作为其变体和片段。