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    SEQUENTIAL MULTIPLEX WESTERN BLOTTING
    104.
    发明申请
    SEQUENTIAL MULTIPLEX WESTERN BLOTTING 审中-公开

    公开(公告)号：US20200209229A1

    公开(公告)日：2020-07-02

    申请号：US16722706

    申请日：2019-12-20

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： William STRONG ,  Eli HEFNER

    IPC: G01N33/543 ,  G01N33/58 ,  C12Q1/6869 ,  C12Q1/6834

    Abstract: Described are methods and compositions for sequential multiplex detection of target analytes in a sample. The method comprises contacting the sample comprising analytes immobilized on a solid support with binding agents that specifically bind an analyte in the sample, wherein each of the binding agents binds a different analyte and is attached to a single-stranded nucleic acid molecule comprising a unique sequence. The sample is then contacted with a labeled complementary nucleic acid molecule that binds the single-stranded nucleic acid molecule attached to one binding agent. The signal from the label is detected, and then reduced or eliminated. The sample can be simultaneously contacted with a second labeled complementary nucleic acid molecule that binds a different binding agent, and the signal from the second label is detected. The process is repeated for each additional analyte in the sample, thereby sequentially detecting the presence of the analytes in the sample.

    Value assignment for customizable quality controls
    105.
    发明授权
    Value assignment for customizable quality controls 有权

    公开(公告)号：US10697981B2

    公开(公告)日：2020-06-30

    申请号：US15849514

    申请日：2017-12-20

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Alireza Ebrahim ,  Karl De Vore ,  Christopher Spates

    IPC: G01N33/96 ,  G01N33/78 ,  G01N33/48

    Abstract: Methods are provided for end users of diagnostic measurement procedures to prepare quality controls having desired analyte recoveries, estimate recoveries of quality controls already prepared, and compare estimated and measured recoveries. To prepare a quality control containing a particular analyte, a desired recovery of a measurement procedure for the analyte can be scaled by a correlation factor to obtain a target nominal concentration of the analyte in the quality control. Alternatively, the nominal concentration of an analyte in a quality control can be scaled by a correlation factor to obtain a predicted recovery of a measurement procedure for the analyte. The correlation factors can be based on recovery data previously obtained using the measurement procedure and optionally one or more reference procedures, and can be calculated using regression analysis of these data. Each quality control can be prepared by dissolving a number of solid beads containing the analyte(s) of interest in a volume of base matrix.

    Microfluidic system with fluid pickups
    106.
    发明授权
    Microfluidic system with fluid pickups 有权

    公开(公告)号：US10682647B2

    公开(公告)日：2020-06-16

    申请号：US15633413

    申请日：2017-06-26

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Anthony J. Makarewicz, Jr. ,  Luc Bousse ,  Stefano Schiaffino

    IPC: G01N1/00 ,  B01L3/00 ,  B01L7/00 ,  C12N15/10 ,  C12Q1/6806

    Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In an exemplary method, a sample-containing fluid may be dispensed into a well through a sample port of a channel component. The channel component may include (a) a body having a bottom surface attached to the well, and a top surface with a microchannel formed therein, and (b) an input tube projecting into the well from the bottom surface of the body. The sample-containing fluid when dispensed may contact a bottom end of the input tube and may be retained, with assistance from gravity, out of contact with the microchannel. A pressure differential may be created that drives at least a portion of the sample-containing fluid from the well via the input tube and through the microchannel.

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