公开(公告)号：US11479814B2

公开(公告)日：2022-10-25

申请号：US16866211

申请日：2020-05-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul Angrish ,  Zhiwei Yang

IPC: C12Q1/68 ,  C12Q1/686 ,  C07D207/16 ,  C07C41/22 ,  C07C211/63 ,  C07C303/28 ,  C07D211/60 ,  C11D1/88 ,  C12N9/12 ,  C12N9/96

Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.

公开(公告)号：US11474070B2

公开(公告)日：2022-10-18

申请号：US16196502

申请日：2018-11-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marcin Sikora ,  Melville Davey ,  Christian Koller ,  Simon Cawley ,  Alan Williams ,  David Kulp

IPC: G01N27/414 ,  G16B30/00 ,  G16B30/10 ,  C12Q1/6869 ,  G01N27/27 ,  G16B30/20

Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.

公开(公告)号：US11473132B2

公开(公告)日：2022-10-18

申请号：US16948442

申请日：2020-09-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shoulian Dong ,  Chunmei Liu

IPC: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6832 ,  C12Q1/6848

Abstract: The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.

公开(公告)号：US11467092B2

公开(公告)日：2022-10-11

申请号：US16797561

申请日：2020-02-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mingsong Chen ,  Mauro Aguanno ,  Kuan Moon Boo ,  Woon Liang Terence Soh ,  Jacob K. Freudenthal ,  Jeffrey A. Marks

IPC: G01N21/64 ,  G01N21/27 ,  G01N21/03

Abstract: An instrument for processing and/or measuring a biological process contains a sample processing system, an excitation source, an excitation optical system, an optical sensor, and an emission optical system. The sample processing system is configured to retain a first sample holder and a second sample holder, wherein the number of sample cells is different for each sample holder or a characteristic dimension for the first sample cells is different from that of the second sample holder. The instrument also includes an excitation source temperature controller comprising a temperature sensor that is coupled to the excitation source. The temperature controller is configured to produce a first target temperature when the first sample holder is retained by the instrument and to produce a second target temperature when the second sample holder is retained by the instrument.

公开(公告)号：US11466328B2

公开(公告)日：2022-10-11

申请号：US16335553

申请日：2017-09-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ann Mongan ,  Alex Atkins

IPC: C12Q1/6886

Abstract: The present invention provides methods and compositions that are useful for assessing gene expression for tumor immune response profile of a sample. In particular, a target-specific primer panel is provided that allows for selective amplification of immune response target sequences in a sample. In one aspect, the invention relates to target-specific primers useful for selective amplification of one or more target sequences associated with immune response. In some aspects, amplified target sequences obtained using the disclosed methods, and compositions can be used in various processes including nucleic acid sequencing and used to detect the presence of genetic variants and/or expression levels of one or more targeted sequences associated with immune response.

公开(公告)号：US11459620B2

公开(公告)日：2022-10-04

申请号：US16706394

申请日：2019-12-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yongmei (Alexis) Ji ,  Pius Brzoska ,  Angela Burrell ,  Craig Cummings ,  Catherine O'Connell

IPC: C12P19/34 ,  C12Q1/689 ,  C12Q1/6806

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

公开(公告)号：US20220220534A1

公开(公告)日：2022-07-14

申请号：US17708813

申请日：2022-03-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Abraham ROSENBAUM ,  Collyn SEEGER ,  Jeremy GRAY ,  Hua YU

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6876

Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

公开(公告)号：US20220195499A1

公开(公告)日：2022-06-23

申请号：US17569245

申请日：2022-01-05

Applicant: Life Technologies Corporation

Inventor： Andrew DIX ,  Jeffrey Monette

IPC: C12Q1/6818 ,  C12Q1/6876

Abstract: The present disclosure is directed to methods and kits for detection and/or quantification of nucleic acids, such as double stranded DNA that is used in next-generation sequencing (NGS) applications. Nucleic acid-based probes, such as peptide nucleic acid (PNA) oligomers, molecular beacons, DNA flares and locked nucleic acid (LNA) oligomers, are also provided for use in the methods and kits of the present disclosure.

公开(公告)号：US11365389B2

公开(公告)日：2022-06-21

申请号：US16903242

申请日：2020-06-16

Applicant: Life TECHNOLOGIES CORPORATION

Inventor： Shawn Barrett ,  Scott Jacobia

IPC: C12P21/02 ,  C12N5/00

Abstract: Cell culture media, concentrated media and feeds, methods of manufacturing cell culture media and feeds, and methods of culturing cells are provided. One or more small peptides, including dipeptides are added to the cell culture media to provide improved stability and improved conditions for culturing cells.

公开(公告)号：US20220187614A1

公开(公告)日：2022-06-16

申请号：US17549114

申请日：2021-12-13

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES HOLDINGS PTE LTD

Inventor： Michael D Ward ,  Gregory Kaduchak ,  Lingzhi ZHU

IPC: G02B27/48 ,  F21V8/00 ,  G02B6/44 ,  G02B6/028

Abstract: Provided are laser sources, the laser sources comprising at least one diode; and an optic fiber of a predefined length disposed between the laser source and a position for a target such that the optic fiber communicates light pulses from the laser source as a source light to the position for the target, wherein the position is illuminated by the source light so as to reduce speckles in a captured image of the target. Also provided are methods for providing source light for generating an image, comprising: generating illumination with one or more laser diodes; and passing the illumination through an optic fiber having a plurality of bends therein such that source light is emitted from the optic fiber so as to illuminate a target with the source light, the source light reducing speckles in an image of the target.