公开(公告)号：US20050255513A1

公开(公告)日：2005-11-17

申请号：US11113837

申请日：2005-04-25

申请人： Ali Akin ,  Elizabeth Bodie ,  Shirley Burrow ,  Nigel Dunn-Coleman ,  Geoffrey Turner ,  Michael Ward

发明人： Ali Akin ,  Elizabeth Bodie ,  Shirley Burrow ,  Nigel Dunn-Coleman ,  Geoffrey Turner ,  Michael Ward

IPC分类号： C07K14/38 ,  C12N9/12 ,  C12N15/80 ,  C12P21/02 ,  C12Q1/68 ,  C07H21/04 ,  C07K14/39 ,  C12N1/18 ,  C12N15/74 ,  C12P21/06

CPC分类号： C12N9/1205 ,  C07K14/38 ,  C12N15/80 ,  C12P21/02

摘要： The present invention generally relates to hyphal growth in fungi and in particular describes the modulation of genes associated with hyphal growth in filamentous fungi. The present invention provides methods and systems for the production of proteins and/or chemicals from filamentous fungi which comprise modulation of genes associated with hyphal growth. Specifically, the present invention is directed to a full length cotA gene, its gene product and methods of use.

摘要翻译： 本发明通常涉及真菌中的菌丝生长，并且具体地描述了丝状真菌中菌丝生长相关基因的调节。 本发明提供了用于从丝状真菌生产蛋白质和/或化学物质的方法和系统，其包括调节与菌丝生长相关的基因。 具体地，本发明涉及全长cotA基因，其基因产物和使用方法。

公开(公告)号：US20050241612A1

公开(公告)日：2005-11-03

申请号：US10511517

申请日：2003-04-19

申请人： Michael Ward

发明人： Michael Ward

IPC分类号： F02B23/08 ,  F02B23/10 ,  F02B31/00 ,  F02B75/04 ,  F02B75/12 ,  F02D37/02 ,  F02F1/38 ,  F02M57/06 ,  F02M67/00 ,  F02M67/02 ,  F02M67/10 ,  F02M69/08 ,  F02N99/00 ,  F02P3/08 ,  F02P9/00 ,  F02P13/00 ,  F02P15/02 ,  F02P15/08

CPC分类号： F02B23/08 ,  F02B23/101 ,  F02B75/044 ,  F02B75/045 ,  F02B2023/085 ,  F02B2023/102 ,  F02B2075/125 ,  F02B2275/40 ,  F02D37/02 ,  F02D2041/389 ,  F02F1/38 ,  F02M57/06 ,  F02M67/02 ,  F02M67/10 ,  F02M69/08 ,  F02N99/002 ,  F02P3/0815 ,  F02P3/0869 ,  F02P9/007 ,  F02P13/00 ,  F02P15/08 ,  Y02T10/123 ,  Y02T10/125

摘要： An improved ignition-engine system for internal combustion engines comprising a compact combustion chamber in the cylinder head and two main squish zones (101 a, 101 b) for producing high flow and turbulence, and at least one minor squish zone (105) at the end of the intake valve (104), the system using one or two independently operated spark plugs (102a, 102b), placed asymmetrically at or near the edge of the high flow squish zones to handle both ultra-lean light load conditions and high load conditions without misfire or knocking, the engine leanness and high load operation further improved by using variable compression ratio and/or direct fuel injection, including air-blast fuel injectors (181) and more centrally located air-blast-ignition fuel injector (193) more ideally suited for four valve engines and mild hybrid engines.

摘要翻译： 一种用于内燃机的改进的点火发动机系统，包括在气缸盖中的紧凑的燃烧室和用于产生高流量和湍流的两个主要挤压区域（101a，101b），以及至少一个小的挤压区域 进气门（104）的端部，使用一个或两个独立操作的火花塞（102a，102b）的系统，其不对称地设置在高流动挤压区域的边缘处或附近，以处理超轻负载条件和 没有失火或爆震的高负载条件，通过使用可变的压缩比和/或直接燃料喷射，包括鼓风燃料喷射器（181）和更中心的鼓风点火燃料喷射器（ 193）更适合四个阀门发动机和温和的混合动力发动机。

公开(公告)号：US20050221369A1

公开(公告)日：2005-10-06

申请号：US11130427

申请日：2005-05-16

申请人： Nigel Dunn-Coleman ,  Frits Goedegebuur ,  Michael Ward ,  Jian Yao

发明人： Nigel Dunn-Coleman ,  Frits Goedegebuur ,  Michael Ward ,  Jian Yao

IPC分类号： C12N15/09 ,  A21D2/26 ,  A21D8/04 ,  A23K1/165 ,  C11D3/386 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/42 ,  C12N15/113 ,  C12Q1/68 ,  C07H21/04 ,  C12N1/18 ,  C12N9/44 ,  C12N15/74

CPC分类号： A21D8/042 ,  C11D3/38645 ,  C12N9/2445 ,  C12N15/1137 ,  C12Y302/01021 ,  Y02E50/17

摘要： The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.

摘要翻译： 本发明提供了命名为bgl3的新型β-葡糖苷酶核酸序列和相应的BGL3氨基酸序列。 本发明还提供了包含编码BGL3的核酸序列，重组BGL3蛋白的表达载体和宿主细胞及其制备方法。

公开(公告)号：US20050158825A1

公开(公告)日：2005-07-21

申请号：US11007886

申请日：2004-12-09

申请人： Scott Power ,  Huaming Wang ,  Michael Ward

发明人： Scott Power ,  Huaming Wang ,  Michael Ward

IPC分类号： C07K16/00 ,  C07K16/28 ,  C07K16/32 ,  C12N1/15 ,  C07K16/46 ,  C12N1/16

CPC分类号： C07K16/2818 ,  C07K16/00 ,  C07K16/32 ,  C07K2317/10 ,  C07K2317/41 ,  C07K2317/55 ,  C07K2317/73 ,  C07K2317/732 ,  C07K2319/00

摘要： Described herein are methods for the production of monoclonal antibodies in filamentous fungi host cells. The monoclonal antibodies are expressed as full-length fusion proteins that retain functional antigen binding and antibody-dependent cellular cytotoxicity capabilities. Improvements in the cleavage of the glucoamylase-light chain fusion protein to yield a mature antibody are also provided. The antibodies produced in filamentous fungi show equivalent pharmacokinetic disposition to antibodies produced in mammalian cells.

摘要翻译： 本文描述了在丝状真菌宿主细胞中生产单克隆抗体的方法。 单克隆抗体表达为全长融合蛋白，其保留功能性抗原结合和抗体依赖性细胞毒性能力。 还提供了葡糖淀粉酶 - 轻链融合蛋白切割产生成熟抗体的改进。 在丝状真菌中产生的抗体对哺乳动物细胞中产生的抗体显示出等效的药代动力学位置。

公开(公告)号：US06562340B1

公开(公告)日：2003-05-13

申请号：US08507362

申请日：1995-08-16

申请人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A Larenas

发明人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A Larenas

IPC分类号： A61K3847

CPC分类号： C12Y302/01004 ,  A23K20/189 ,  C12N9/2437 ,  C12N15/80 ,  C12Y302/01021 ,  C12Y302/01091

摘要： The use is provided of a composition as a feed additive which comprises one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. Such enzyme-based feed additives can be incorporated into a cereal-based feed which includes one or more of barley, wheat, tricale, rye and maize. The feed additive has the advantage of improving the feed conversion ratio and/or increasing the digestibility of a cereal-based feed in which it is included.

摘要翻译： 提供的组合物作为饲料添加剂，其包含一种或多种内切葡聚糖酶，并且基于组合物中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 所述凝集素可以是一种或多种EGI，EGII EGIII及其任何功能活性的衍生物。 这样的内切葡聚糖酶可以从真菌木霉的遗传修饰菌株获得。 还提供了一种基于酶的饲料添加剂，其包含缺乏纤维素结合结构域的EGI和/或EGII，并且基于添加剂中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 提供了另外一种基于酶的饲料添加剂，其包含基于谷物的载体，一种或多种内切葡聚糖酶，和基于添加剂中的纤维素酶蛋白质含量为0-20重量％的纤维二糖水解酶。 这种基于酶的饲料添加剂可以掺入包括一种或多种大麦，小麦，三倍体，黑麦和玉米的谷类饲料中。 饲料添加剂具有提高饲料转化率和/或增加其中包含谷物饲料的消化率的优点。

公开(公告)号：US06458928B1

公开(公告)日：2002-10-01

申请号：US09112498

申请日：1998-07-09

申请人： Barbara A. Swanson ,  Michael Ward ,  Merja Penttilä ,  Jaakko Pere ,  Markku Saloheimo

发明人： Barbara A. Swanson ,  Michael Ward ,  Merja Penttilä ,  Jaakko Pere ,  Markku Saloheimo

IPC分类号： C07K1400

CPC分类号： D21H21/14 ,  A23K10/12 ,  A23K20/147 ,  C07K14/37 ,  C11D3/386 ,  D06M16/003 ,  D06M2101/06 ,  D21H17/005

摘要： A novel microbial protein is described which appears to have significant homology to plant expansin proteins and has the ability to weaken filter paper and swell cellulose. A DNA is described which encodes the novel protein.

摘要翻译： 描述了一种似乎与植物扩展蛋白质具有显着同源性的新型微生物蛋白质，并具有削弱滤纸和溶胀纤维素的能力。 描述编码新蛋白质的DNA。

公开(公告)号：US5874276A

公开(公告)日：1999-02-23

申请号：US448873

申请日：1995-05-24

申请人： Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund Larenas

发明人： Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund Larenas

IPC分类号： C12N15/09 ,  A23K1/165 ,  C12N1/15 ,  C12N9/42 ,  C12N15/31 ,  C12N15/80 ,  C12R1/885

CPC分类号： C12Y302/01004 ,  A23K20/189 ,  C12N15/80 ,  C12N9/2437 ,  C12Y302/01021 ,  C12Y302/01091

摘要： The present invention relates to the cloning and high level expression of novel truncated cellulase proteins or derivatives thereof in the filamentous fungus Trichoderma longibrachiatum. Further aspects of the present invention relate to fungal transformants that express the novel truncated cellulases and derivatives, and expression vectors comprising the DNA gene fragments or variants thereof that code for the truncated cellulases derived from Trichoderma longibrachiatum using genetic engineering techniques.

公开(公告)号：US5753484A

公开(公告)日：1998-05-19

申请号：US438870

申请日：1995-05-10

申请人： Michael Ward ,  Kathleen A. Clarkson ,  Edmund A. Larenas ,  Jeffrey D. Lorch ,  Geoffrey L. Weiss

发明人： Michael Ward ,  Kathleen A. Clarkson ,  Edmund A. Larenas ,  Jeffrey D. Lorch ,  Geoffrey L. Weiss

IPC分类号： C12N15/09 ,  C11D3/386 ,  C11D11/00 ,  C12N9/24 ,  C12N9/42 ,  C12N15/56 ,  C12N15/80 ,  C12R1/885 ,  D06M16/00 ,  C07K1/00 ,  C12N9/00 ,  C12N9/14

CPC分类号： C11D3/38645 ,  C12N15/80 ,  C12N9/2437 ,  C12Y302/01004 ,  D06M16/003 ,  D06M2200/50

摘要： The present invention is directed to purified EG III cellulase enzyme isolated from Trichoderma longibrachiatum and the amino acid sequence of the secreted (mature) and non-secreted (preprotein) forms. The present invention is further directed to the DNA fragment and sequence that encodes the EG III cellulase enzyme. Also disclosed are methods for isolating either purified or highly enriched EG III cellulase obtained from Trichoderma spp. or genetically modified strains of Trichoderma spp.

摘要翻译： 本发明涉及从木霉木霉分离的纯化的EG III纤维素酶和分泌（成熟）和非分泌（蛋白质）形式的氨基酸序列。 本发明还涉及编码EG III纤维素酶的DNA片段和序列。 还公开了分离从木霉属（Trichoderma spp）获得的纯化的或高度富集的EG III纤维素酶的方法。 或木霉属的遗传修饰菌株。

公开(公告)号：US20140123404A1

公开(公告)日：2014-05-08

申请号：US14110123

申请日：2012-03-20

申请人： Huaming Wang ,  Michael Ward

发明人： Huaming Wang ,  Michael Ward

IPC分类号： C12N9/02 ,  D06L3/11

CPC分类号： C12N9/0061 ,  D06L4/40

摘要： The present compositions, methods, and systems, relating to variant laccase enzymes that demonstrate increased expression and/or activity compared to a reference parental laccase enzyme. The variant enzymes include mutations that affect glycosylation, surface charge, or surface hydrophobicity, resulting in improved enzyme expression and/or enzyme activity.

摘要翻译： 与参照亲本漆酶相比表现出增加的表达和/或活性的变体漆酶的本发明组合物，方法和系统。 变体酶包括影响糖基化，表面电荷或表面疏水性的突变，导致改善的酶表达和/或酶活性。

公开(公告)号：US08715647B2

公开(公告)日：2014-05-06

申请号：US11198426

申请日：2005-08-05

申请人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A. Larenas

发明人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A. Larenas

IPC分类号： A23K1/165 ,  A61K38/54 ,  A61K38/47 ,  A61K38/46 ,  A61K38/00

CPC分类号： C12Y302/01004 ,  A23K20/189 ,  A23K50/75 ,  C12N9/2437 ,  C12Y302/01091

摘要： The use is provided composition as a feed additive which comprises one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII, EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase.

摘要翻译： 提供的组合物作为饲料添加剂，其包含一种或多种内切葡聚糖酶，并且基于组合物中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 所述凝集素可以是EGI，EGII，EGIII及其任何的任何功能活性衍生物中的一种或多种。 这样的内切葡聚糖酶可以从真菌木霉的遗传修饰菌株获得。 还提供了一种基于酶的饲料添加剂，其包含缺乏纤维素结合结构域的EGI和/或EGII，并且基于添加剂中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 提供了另外一种基于酶的饲料添加剂，其包含基于谷物的载体，一种或多种内切葡聚糖酶，和基于添加剂中的纤维素酶蛋白质含量为0-20重量％的纤维二糖水解酶。