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360 条记录 (耗时 0.026 秒)

    Trehalose phosphorylase, its preparation and uses
    101.
    发明授权
    Trehalose phosphorylase, its preparation and uses 失效
    海藻糖磷酸化酶，其制备及用途

    公开(公告)号：US5910436A

    公开(公告)日：1999-06-08

    申请号：US102644

    申请日：1998-06-23

    申请人： Tetsuya Nakada ,  Michio Kubota ,  Hiroto Chaen ,  Toshio Miyake

    发明人： Tetsuya Nakada ,  Michio Kubota ,  Hiroto Chaen ,  Toshio Miyake

    IPC分类号： A61K38/00 ,  C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12P19/18 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N9/1051 ,  C12P19/18 ,  A61K38/00

    摘要： A thermostable trehalose phosphorylase which is obtainable from microorganisms of the genus Thermoanaerobium and which hydrolyzes trehalose in the presence of an inorganic phosphoric acid to form D-glucose and .beta.-D-glucose-1-phosphoric acid. The trehalose phosphorylase can be also prepared by recombinant DNA technology. When the enzyme is allowed to contact with .beta.-D-glucose-1-phosphoric acid as a saccharide donor in the presence of other saccharides, glucosyl-transferred saccharides including glucosyl-D-galactoside, which are conventionally known but scarcely obtainable, can be produced on an industrial-scale and in a relatively-low cost.

    摘要翻译： 一种热稳定的海藻糖磷酸化酶，其可从Thermoanaerobium属的微生物获得，并且在无机磷酸存在下水解海藻糖以形成D-葡萄糖和β-D-葡萄糖-1-磷酸。 海藻糖磷酸化酶也可以通过重组DNA技术制备。 当酶在其他糖类存在下与β-D-葡萄糖-1-磷酸作为糖供体接触时，通常已知但几乎不可获得的包括葡糖基-D-半乳糖苷的葡糖基转移的糖可以是 以工业规模生产，成本相对较低。

    DNA encoding enzyme, recombinant DNA and enzyme, transformant, and their preparation and uses
    105.
    发明授权
    DNA encoding enzyme, recombinant DNA and enzyme, transformant, and their preparation and uses 失效
    DNA编码酶，重组DNA和酶，转化体及其制备和用途

    公开(公告)号：US5871994A

    公开(公告)日：1999-02-16

    申请号：US714537

    申请日：1996-09-16

    申请人： Michio Kubota ,  Keiji Tsusaki ,  Kazuhiko Maruta ,  Toshiyuki Sugimoto

    发明人： Michio Kubota ,  Keiji Tsusaki ,  Kazuhiko Maruta ,  Toshiyuki Sugimoto

    IPC分类号： C12N1/21 ,  C12N9/90 ,  C12N15/56 ,  C12P19/12 ,  C12N9/24 ,  C12N9/26

    CPC分类号： C12P19/12 ,  C12N9/90

    摘要： A DNA encoding an enzyme, which forms non-reducing saccharides having trehalose structure as an end unit from amylaceous saccharides having a degree of glucose polymerization of 3 or higher, enables an industrial-scale production of a recombinant enzyme with such enzyme activity. Non-reducing saccharides obtainable by the recombinant enzyme can be used in a variety of food products, cosmetics, pharmaceuticals and feeds because of their substantial non-reducibility, mild and high-quality sweetness, adequate viscosity, and moisture-retaining ability.

    摘要翻译： 从葡萄糖聚合度为3以上的淀粉糖形成具有海藻糖结构的非还原性糖类作为末端单元的酶的DNA能够实现具有这种酶活性的重组酶的工业规模生产。 可以通过重组酶获得的非还原性糖可以用于各种食品，化妆品，药​​物和饲料中，因为它们具有显着的不可还原性，温和和高品质的甜度，足够的粘度和保湿能力。

    Non-reducing saccharides, their preparation and use
    106.
    发明授权
    Non-reducing saccharides, their preparation and use 失效
    非还原糖，其制备和用途

    公开(公告)号：US5858992A

    公开(公告)日：1999-01-12

    申请号：US962880

    申请日：1997-11-04

    申请人： Tomoyuki Nishimoto ,  Hiroto Chaen ,  Toshiyuki Sugimoto ,  Toshio Miyake

    发明人： Tomoyuki Nishimoto ,  Hiroto Chaen ,  Toshiyuki Sugimoto ,  Toshio Miyake

    IPC分类号： C07H3/06 ,  C12P19/04 ,  A61K31/715 ,  A01N43/04 ,  C07H1/00

    CPC分类号： C07H3/06 ,  C12P19/04

    摘要： .alpha.-Isomaltosyl .alpha.-isomaltoside represented by O-.alpha.-D-glucopyranosyl -(1.fwdarw.6)-.alpha.-D-glucopyranosyl O-.alpha.-D-glucopyranosyl-(1.fwdarw.6) -.alpha.-D-glucopyranoside is a novel non-reducing saccharide which promotes the growth of bifid bacteria, has an anti-caries activity, and promotes the mineral absorption. Similarly as the non-reducing saccharide, .alpha.-isomaltosyl .alpha.-glucoside and .alpha.-isomaltotriosyl .alpha.-glucoside can be also used to prevent caries and promote the growth of bifid bacteria and the mineral absorption. These saccharides can be used alone or in combination in a variety of foods, cosmetics and pharmaceuticals.

    摘要翻译： 由α-D-吡喃葡萄糖基 - （1-> 6）-β-D-吡喃葡萄糖基-O-α-D-吡喃葡萄糖基 - （1-> 6）-β-D-吡喃葡萄糖苷代表的α-甲氧咪唑烷基α-异麦芽糖苷是 促进双歧杆菌生长的新型非还原性糖类具有抗龋齿活性，促进矿物质吸收。 类似地，作为非还原性糖，α-异麦芽糖基α-葡萄糖苷和α-异麦芽糖基α-葡糖苷也可用于预防龋齿并促进双歧杆菌的生长和矿物质吸收。 这些糖类可以单独使用，也可以组合使用于各种食品，化妆品，药​​品等。

    Recombinant enzyme for converting maltose into trehalose from pimelobacter sp.
    108.
    发明授权
    Recombinant enzyme for converting maltose into trehalose from pimelobacter sp. 失效
    用于从麦芽糖转化成麦芽糖至海藻糖的重组酶。

    公开(公告)号：US5763228A

    公开(公告)日：1998-06-09

    申请号：US528199

    申请日：1995-09-14

    申请人： Michio Kubota ,  Keiji Tsusaki ,  Toshiyuki Sugimoto

    发明人： Michio Kubota ,  Keiji Tsusaki ,  Toshiyuki Sugimoto

    IPC分类号： C12N9/90 ,  C12P19/24 ,  C12P19/12 ,  C12N9/24 ,  C12N15/56

    CPC分类号： C12N9/90 ,  C12P19/24

    摘要： A recombinant enzyme, having a molecular weight of about 57,000-67,000 daltons on SDS-PAGE and a pI of about 4.1-5.1 on isoelectrophoresis, which converts maltose into trehalose and vice versa. Depending on the enzymatic conditions, the enzyme forms about 70 w/w % of trehalose when acts on maltose, while about 20 w/w % of maltose when acts on trehalose. The culture of a transformant, prepared by introducing into a host a recombinant DNA containing a DNA coding for the enzyme and a self-replicable vector, facilitates the industrial-scale production of trehalose.

    摘要翻译： 重组酶，SDS-PAGE上分子量约57,000-67,000道尔顿，在电泳上的pI约为4.1-5.1，将麦芽糖转化为海藻糖，反之亦然。 根据酶的条件，当作用于麦芽糖时，该酶形成约70w / w％的海藻糖，而当作用于海藻糖时约为20w / w％的麦芽糖。 通过向宿主中引入含有编码酶的DNA和自我复制载体的重组DNA制备的转化体的培养有利于海藻糖的工业规模生产。

    Recombinant DNA and transformant containing the same
    109.
    发明授权
    Recombinant DNA and transformant containing the same 失效
    重组DNA和含有其的转化体

    公开(公告)号：US5731193A

    公开(公告)日：1998-03-24

    申请号：US828511

    申请日：1997-03-31

    申请人： Tetsuya Mori ,  Kozo Yamamoto ,  Tsunetaka Ohta

    发明人： Tetsuya Mori ,  Kozo Yamamoto ,  Tsunetaka Ohta

    IPC分类号： C12N15/09 ,  C07K14/505 ,  C07K14/56 ,  C12N5/10 ,  C12N15/85 ,  C07H21/04 ,  C12N15/67 ,  C12P21/02

    CPC分类号： C12N15/85 ,  C07K14/505 ,  C07K14/56 ,  C12N2830/00 ,  C12N2830/42 ,  C12N2830/85

    摘要： Insertion of IFN-alpha promoters in recombinant DNAs improves their expression efficiencies for useful polypeptides. Expression of such a recombinant DNA in host cells of mammalian origin is artificially controllable by the presence and absence of external stimuli using IFN-alpha inducers. Thus, transformants with such a recombinant DNA readily increase to a maximized cell density with causing neither damages nor extinction due to polypeptides they produce, and subsequent exposure to IFN-alpha inducers allows the proliferated cells to efficiently produce polypeptides with significant glycosylations.

    摘要翻译： 在重组DNA中插入IFN-α启动子改善了其对有用多肽的表达效率。 这种重组DNA在哺乳动物来源的宿主细胞中的表达可以通过使用IFN-α诱导物的外部刺激的存在和不存在来人为可控。 因此，具有这种重组DNA的转化体容易增加到最大化的细胞密度，由于它们产生的多肽而不造成损伤或消灭，随后暴露于IFN-α诱导物允许增殖的细胞有效地产生具有显着糖基化的多肽。