公开(公告)号：US09228196B2

公开(公告)日：2016-01-05

申请号：US14067317

申请日：2013-10-30

Applicant: Academia Sinica

Inventor： Yi-Fang Tsay ,  Shu-Chun Fan ,  Hui-Yu Chen ,  Kuo-En Chen

IPC: C12N5/14 ,  C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8243 ,  C07K14/415 ,  C12N15/8261 ,  Y02A40/146

Abstract: The present invention provides a method for changing nitrogen utilization efficiency in a plant comprises regulating the expression of Arabidopsis NRT 1.7 or an ortholog thereof so that the nitrate remobilization from older leaves to young leaves in the plant is regulated, thereby the nitrogen utilization efficiency is changed. The present invention also provides a transgenic plant obtainable by transforming a plant with an expression construct with a high or low level of expression of NRT 1.7. On the other hand, the present invention yet provides a chimera nitrate transporter, a DNA molecule coding for this chimera transporter and an expression vector thereof.

Abstract translation: 本发明提供一种改变植物氮利用效率的方法，包括调节拟南芥NRT 1.7或其直系同源物的表达，从而调节植物中从老叶到幼叶的硝酸盐再生，从而改变氮利用效率 。 本发明还提供了通过用具有高或低水平的NRT1.7表达的表达构建体转化植物可获得的转基因植物。 另一方面，本发明还提供了嵌合体硝酸盐转运蛋白，编码该嵌合体转运蛋白的DNA分子及其表达载体。

公开(公告)号：US20150377887A1

公开(公告)日：2015-12-31

申请号：US14769294

申请日：2014-02-27

Applicant: Yu-cheng SU ,  Steve R. ROFFLER ,  ACADEMIA SINICA

Inventor： Yu-Cheng Su ,  Steve R. ROFFLER

IPC: G01N33/577 ,  C07K16/40 ,  C07K16/44 ,  G01N33/68 ,  G01N33/50

CPC classification number: G01N33/577 ,  C07K16/00 ,  C07K16/40 ,  C07K16/44 ,  C07K2317/14 ,  G01N33/5008 ,  G01N33/6854

Abstract: This disclosure relates to a method to mimicking the germinal center reaction to generate antibodies with altered binding properties or increased affinity directly in hybridomas. To allow convenient and rapid affinity maturation of antibodies, a controllable activation-induced cytidine deaminase (AID) expression system to induce somatic hypermutation in hybridomas is developed. Selection of high affinity antibodies is achieved by fluorescence-activated cell sorting of hybridomas that preferentially bind fluorescence-labeled antigens. The disclosure also relates to de novo generation of hybridomas with tunable antibody affinity by generating myeloma fusion partners with controllable AID expression.

Abstract translation: 本公开涉及模拟生发中心反应以产生具有改变的结合特性或直接在杂交瘤中增加的亲和力的抗体的方法。 为了允许抗体的方便和快速的亲和力成熟，开发了可控的活化诱导的胞苷脱氨酶（AID）表达系统以诱导杂交瘤中的体细胞超突变。 通过优先结合荧光标记的抗原的杂交瘤的荧光激活细胞分选来实现高亲和力抗体的选择。 本公开还涉及通过产生具有可控AID表达的骨髓瘤融合配偶体从而产生具有可调节抗体亲和力的杂交瘤。

公开(公告)号：US20150369806A1

公开(公告)日：2015-12-24

申请号：US14742305

申请日：2015-06-17

Applicant: National Chung Hsing University ,  Academia Sinica

Inventor： GOU-JEN WANG ,  Shie-Liang Hsieh ,  Yen-Ting Tung

IPC: G01N33/569 ,  G01N33/543 ,  C25D3/48 ,  C23C14/34 ,  C23C14/18 ,  G01N33/553 ,  G01N27/12

CPC classification number: G01N33/56983 ,  C23C14/18 ,  C23C14/34 ,  C25D11/04 ,  C25D11/24 ,  G01N27/026 ,  G01N27/127 ,  G01N33/54346 ,  G01N33/54366 ,  Y02A50/53 ,  Y02A50/60

Abstract: A sensor for detecting molecular interactions between a target and a binding domain by electrochemical impedance spectroscopy. The sensor includes an anodic aluminum oxide barrier layer having a gold-coated array of regularly spaced nano-hemispheres and gold nanoparticles coated with a binding domain attached thereto. Also provided are methods for producing the sensor and for using the sensor to detect the presence of a virus in a sample.

Abstract translation: 用于通过电化学阻抗光谱法检测靶和结合结构域之间的分子相互作用的传感器。 传感器包括阳极氧化铝阻挡层，其具有规则间隔的纳米半球的金涂层阵列和涂覆有附着于其上的结合结构域的金纳米颗粒。 还提供了用于产生传感器和使用传感器来检测样品中病毒的存在的方法。

公开(公告)号：US20150344900A1

公开(公告)日：2015-12-03

申请号：US14759529

申请日：2013-11-21

Applicant: ACADEMIA SINICA

Inventor： Su-May Yu ,  Yi-Shih Chen ,  Shuen-Fang Lo ,  Tuan-Hua David Ho

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8261 ,  C07K14/415 ,  C12N15/8271 ,  C12N15/8293 ,  Y02A40/146

Abstract: The present invention relates to a method for enhancing root growth of a plant by introducing a polynucleotide encoding late embryogenesis abundant protein, group 3 (LEA3) into the plant. Plant root architecture is essential for its functions in water and nutrient uptake, anchorage and interactions with microbes in the soil.

Abstract translation: 本发明涉及通过将编码晚期胚胎发生丰富蛋白质第3组（LEA3）的多核苷酸引入植物来提高植物根系生长的方法。 植物根系结构对于其在水中的功能和养分吸收，锚定和与土壤中微生物的相互作用至关重要。

公开(公告)号：US20150335728A1

公开(公告)日：2015-11-26

申请号：US14182296

申请日：2014-02-18

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Che MA ,  Cheng-Chi WANG ,  Juine-Ruey CHEN

IPC: A61K39/145 ,  C12N7/00

CPC classification number: A61K39/145 ,  A61K39/12 ,  A61K2039/55505 ,  C07K16/1018 ,  C07K2317/76 ,  C12N7/00 ,  C12N2740/11034 ,  C12N2740/16034 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2760/16171 ,  C12N2770/24134 ,  Y02A50/386

Abstract: Immunogenic compositions comprising partially glycosylated viral glycoproteins for use as vaccines against viruses are provided. Vaccines formulated using mono-, di-, or tri-glycosylated viral surface glycoproteins and polypeptides provide potent and broad protection against viruses, even across strains. Pharmaceutical compositions comprising monoglycosylated hemagglutinin polypeptides and vaccines generated therefrom and methods of their use for prophylaxis or treatment of viral infections are disclosed. Methods and compositions are disclosed for influenza virus HA, NA and M2, RSV proteins F, G and SH, Dengue virus glycoproteins M or E, hepatitis C virus glycoprotein E1 or E2 and HIV glycoproteins gp120 and gp41.

Abstract translation: 提供了包含部分糖基化的病毒糖蛋白的免疫原性组合物，其用作抗病毒疫苗。 使用单糖，二糖或三糖基化的病毒表面糖蛋白和多肽配制的疫苗甚至可以跨毒株提供有效和广泛的防病毒保护。 公开了包含单糖基化血凝素多肽和由其产生的疫苗的药物组合物及其用于预防或治疗病毒感染的方法。 公开了用于流感病毒HA，NA和M2，RSV蛋白F，G和SH，登革病毒糖蛋白M或E，丙型肝炎病毒糖蛋白E1或E2和HIV糖蛋白gp120和gp41的方法和组合物。

公开(公告)号：US09193665B2

公开(公告)日：2015-11-24

申请号：US14287858

申请日：2014-05-27

Applicant: Academia Sinica

Inventor： Rong-Jie Chein

IPC: C07C69/96 ,  C07C47/36 ,  C07C69/753 ,  C07C35/37 ,  C07C49/753 ,  C07C69/757 ,  C07C49/757 ,  C07C62/32 ,  C07C29/15 ,  C07C45/00 ,  C07C45/41 ,  C07C67/30 ,  C07C253/00 ,  C07C253/30 ,  C07C259/06 ,  C07C271/24 ,  C07C275/28 ,  C07C211/31 ,  C07C215/42 ,  C07C321/20 ,  C07C323/15 ,  C07C225/14 ,  C07C323/22 ,  C07C323/28 ,  C07C327/06 ,  C07C327/16 ,  C07C33/16 ,  C07C33/44 ,  C07C69/24 ,  C07C69/33 ,  C07C69/587 ,  C07C47/46 ,  C07F9/117 ,  C07C22/02 ,  C07D303/46 ,  C07C69/007

CPC classification number: C07C69/96 ,  C07C22/02 ,  C07C29/15 ,  C07C33/16 ,  C07C33/44 ,  C07C35/37 ,  C07C45/00 ,  C07C45/41 ,  C07C47/36 ,  C07C47/46 ,  C07C49/757 ,  C07C62/32 ,  C07C67/30 ,  C07C69/007 ,  C07C69/24 ,  C07C69/33 ,  C07C69/587 ,  C07C69/757 ,  C07C211/31 ,  C07C215/42 ,  C07C225/14 ,  C07C253/00 ,  C07C253/30 ,  C07C259/06 ,  C07C271/24 ,  C07C275/28 ,  C07C321/20 ,  C07C323/15 ,  C07C323/22 ,  C07C323/28 ,  C07C327/06 ,  C07C327/16 ,  C07C2603/30 ,  C07D303/46 ,  C07F9/117

Abstract: The present disclosure provides methods of preparing glucagon-like peptide-1 (GLP-1) receptor modulators, such as compounds of Formula (II) or (VI), or intermediates thereof. The compounds that may be prepared by the described methods are useful for regulating blood glucose levels and/or treating a disease mediated by a GLP-1 receptor (e.g., diabetes).

Abstract translation: 本公开提供了制备胰高血糖素样肽-1（GLP-1）受体调节剂的方法，例如式（II）或（VI）的化合物或其中间体。 可以通过所述方法制备的化合物可用于调节血糖水平和/或治疗由GLP-1受体介导的疾病（例如糖尿病）。

公开(公告)号：US20150284735A1

公开(公告)日：2015-10-08

申请号：US14606159

申请日：2015-01-27

Applicant: Academia Sinica

Inventor： SU-MAY YU ,  CHIEN-RU LIN

IPC: C12N15/82

CPC classification number: C12N15/8245 ,  C12N15/8218 ,  C12N15/8261 ,  Y02A40/146

Abstract: The present invention provides relates to a SKIN gene silencing plasmid comprising a promoter; two DNA fragments, which are obtained from one DNA fragment derived from the cDNA of SKIN1 or SKIN2 and arranged in sense and antisense orientation; and a third DNA fragment inserted between the two DNA fragments. SKIN is a negative regulator of the growth and yield of rice, and decreasing the expression of endogenous SKIN increases the yield of rice. The present invention also relates to a transformed plant cell and a transgenic plant comprising the SKIN gene silencing plasmid.

Abstract translation: 本发明涉及包含启动子的SKIN基因沉默质粒; 两个DNA片段，其从衍生自SKIN1或SKIN2的cDNA的一个DNA片段获得并以正义和反义方向排列; 和插入两个DNA片段之间的第三个DNA片段。 SKIN是水稻生长和产量的负调节因子，降低内源性SKIN的表达增加了水稻的产量。 本发明还涉及转化的植物细胞和包含SKIN基因沉默质粒的转基因植物。

公开(公告)号：US20150273034A1

公开(公告)日：2015-10-01

申请号：US14675838

申请日：2015-04-01

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Chung-Yi Wu ,  Alice L. Yu ,  John Yu

IPC: A61K39/00

CPC classification number: A61K39/0011 ,  A61K2039/55511 ,  A61K2039/6037 ,  A61K2039/6081 ,  A61K2039/627 ,  C12N15/1137 ,  C12N2310/14

Abstract: An immunogenic composition containing a glycan conjugate including a carrier protein, and a glycan including Globo H, an immunogenic fragment thereof, or stage-specific embryonic antigen-4 (SSEA-4), wherein the glycan is conjugated with the carrier protein through a linker.

Abstract translation: 含有载体蛋白的聚糖缀合物和包含Globo H，其免疫原性片段或阶段特异性胚胎抗原-4（SSEA-4）的聚糖的免疫原性组合物，其中所述聚糖通过接头与载体蛋白缀合 。

公开(公告)号：US09124387B2

公开(公告)日：2015-09-01

申请号：US13900637

申请日：2013-05-23

Applicant: Academia Sinica

Inventor： Kate Ching-Ju Lin ,  Wei-Liang Shen ,  Shyamnath Gollakota ,  Dina Katabi

IPC: H04W4/00 ,  H04L1/00

CPC classification number: H04L1/0002 ,  H04B7/0452 ,  H04L1/0015 ,  H04L1/06

Abstract: Dynamic adaption of transmission rate for multiuser MIMO networks is disclosed. The invention controls a first communication device to transmit data at rates optimal determined under the condition that the channel is occupied by the first communication device, a second communication device to transmit data at rates optimal determined under the condition that the channel is shared by the first and second communication devices and a third communication to transmit data at rates optimal determined under the condition that the channel is shared by the first, second and third communication device.

Abstract translation: 公开了多用户MIMO网络的传输速率的动态适应性。 本发明控制第一通信设备以在该信道被第一通信设备占用的条件下最佳确定的速率发送数据;第二通信设备，用于以最佳的速率发送数据，该信道在该信道被第一通信设备共享的条件下确定 以及第二通信设备和第三通信，以在由第一通信设备，第二通信设备和第三通信设备共享信道的条件下最佳确定的速率发送数据。

公开(公告)号：US20150232417A1

公开(公告)日：2015-08-20

申请号：US14188678

申请日：2014-02-24

Applicant: ACADEMIA SINICA

Inventor： Chi-Huey WONG ,  Che Alex MA ,  Ting-Jen Rachel CHENG ,  Wei-Chieh CHENG

IPC: C07C235/64 ,  G01N33/573

CPC classification number: C07C235/64 ,  C07C235/84 ,  C07K2299/00 ,  C12Q1/18 ,  C12Q1/48 ,  G01N33/573 ,  G01N2333/91102 ,  G01N2500/00 ,  G01N2500/04 ,  G01N2500/20

Abstract: Crystal structure at 2.16 Å resolution of full-length Escherichia coli penicillin-binding protein 1b (PBP1b) in complex with its inhibitor moenomycin, is provided. 3D structures of amino acid residues involved in moenomycin binding and transglycosylation activity are identified. Binding sites for peptidoglycan synthesis inhibitors comprising amino acid residues from transglycosylase (TG), UvrB domain 2 homolog (UB2H) and transmembrane (TM) domains of PBP1b are identified at atomic level resolution. Rational drug design, based on the atomic coordinates, are disclosed. Methods for screening for antibiotics using anisotropic binding and transglycosylase inhibitor assays and novel antibiotics based on the screening assays are provided.

Abstract translation: 提供了全长大肠杆菌青霉素结合蛋白1b（PBP1b）与其抑制剂新霉素复合物的分辨率为2.16Å的晶体结构。 鉴定涉及霉酚霉素结合和转糖基化活性的氨基酸残基的3D结构。 在原子级分辨率下鉴定了包含来自转糖苷酶（TG），UvrB结构域2同源物（UB2H）和PBP1b跨膜（TM）结构域的氨基酸残基的肽聚糖合成抑制剂的结合位点。 公开了基于原子坐标的合理药物设计。 提供了使用各向异性结合和转糖苷酶抑制剂测定法筛选抗生素的方法以及基于筛选试验的新型抗生素。