公开(公告)号：US20040121454A1

公开(公告)日：2004-06-24

申请号：US10633448

申请日：2003-08-01

Applicant: BioProcessors Corp.

Inventor： Andrey Zarur Jury ,  Mark D. Angelino

IPC: C12M001/00

CPC classification number: B01F13/0064 ,  B01F5/0256 ,  B01F5/061 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0094 ,  B01F15/00935 ,  B01F2005/0621 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/00835 ,  B01J2219/00862 ,  B01J2219/00871 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00907 ,  B01J2219/00957 ,  B01J2219/00961 ,  B01J2219/00963 ,  B01J2219/00966 ,  C12M23/16

Abstract: Chemical and biological reactors, including microreactors, are provided. Exemplary reactors include a plurality of reactors operable in parallel, where each reactor has a small volume and, together, the reactors produce a large volume of product. Reaction systems can include mixing chambers, heating/dispersion units, reaction chambers, and separation units. Components of the reactors can be readily formed from a variety of materials. For example, they can be etched from silicon. Components are connectable to and separable from each other to form a variety of types of reactors, and the reactors can be attachable to and separable from each other to add significant flexibility in parallel and/or series reactor operation.

Abstract translation: 提供化学和生物反应堆，包括微反应器。 示例性反应器包括可并行操作的多个反应器，其中每个反应器具有小体积，并且一起反应器产生大体积的产物。 反应系统可以包括混合室，加热/分散单元，反应室和分离单元。 反应器的组分可以容易地由各种材料形成。 例如，它们可以从硅上蚀刻。 组件可彼此连接并可分离以形成各种类型的反应器，并且反应器可以彼此附接和分离，以在并联和/或串联反应器操作中增加显着的灵活性。

公开(公告)号：US20070048863A1

公开(公告)日：2007-03-01

申请号：US11492445

申请日：2006-07-25

Applicant: Seth Rodgers ,  Fan Zhang ,  Mohamed Shaheen ,  Benjamin Alexander

Inventor： Seth Rodgers ,  Fan Zhang ,  Mohamed Shaheen ,  Benjamin Alexander

IPC: C12N5/06 ,  G06F19/00

CPC classification number: C12M41/48 ,  G16B50/00

Abstract: Computer-facilitated design of large-scale, multi-factorial cell culture experiments and the like, and control of reaction sites and/or arrays of reaction sites to perform such experiments using automated devices. In certain cases, the invention is directed to controlling a plurality of cell culture experiments, e.g., using an automated cell culture device. In one set of embodiments, a data structure or a “descriptor” for use with cell culture experiments is provided. The descriptor may be used, for instance, to control one or more cell culture experiments, to identify one or more cell culture experiments, and/or to identify or “tag” data arising from one or more cell culture experiments, e.g., for further analysis or recall.

Abstract translation: 大规模，多因素细胞培养实验等的计算机辅助设计，以及反应位点和/或反应位点阵列的控制，以使用自动化装置进行这种实验。 在某些情况下，本发明涉及控制多种细胞培养实验，例如使用自动细胞培养装置。 在一组实施例中，提供了用于细胞培养实验的数据结构或“描述符”。 描述符可以用于例如控制一个或多个细胞培养实验，以鉴定一个或多个细胞培养实验，和/或识别或“标记”由一个或多个细胞培养实验产生的数据，例如进一步 分析或召回。

公开(公告)号：US20070036690A1

公开(公告)日：2007-02-15

申请号：US11146922

申请日：2005-06-07

Applicant: Scott Miller ,  A. Russo ,  Sean LeBlanc ,  Timothy Johnson

Inventor： Scott Miller ,  A. Russo ,  Sean LeBlanc ,  Timothy Johnson

IPC: B01J19/00 ,  B01L3/00

CPC classification number: B01J19/0093 ,  B01J2219/00837 ,  B01J2219/00862 ,  B01J2219/00869 ,  B01J2219/00907 ,  B01J2219/00952 ,  B01J2219/00963 ,  B01J2219/0097 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502746 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0825 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/10 ,  B01L2400/0457 ,  B01L2400/0688 ,  B01L2400/086

Abstract: The present invention generally relates to chemical, biological, and/or biochemical reactor microreactors and other reaction systems such as microreactor systems, as well as systems and methods for constructing and using such devices. In one aspect, a reactor on a chip has a container in fluid communication with a channel, and the channel is in fluid communication with a port for connecting the container to a source of fluid to be introduced into the container. The container can be very small, for example, with a volume of less than about 2 milliliters, and the fluid channel can have a channel volume of less than 1.5 percent of the container volume. According to another aspect, the combined volume of the port volume and the channel volume can be less than about 10 percent of the container volume. Such a configuration may increase the percentage of added fluid that reaches the container. In fed-batch operations, species may be added and removed via the same channel so that a gas headspace can be maintained within the reactor.

Abstract translation: 本发明一般涉及化学，生物和/或生化反应器微反应器和其它反应系统，例如微反应器系统，以及用于构建和使用这些装置的系统和方法。 在一个方面，芯片上的反应器具有与通道流体连通的容器，并且通道与用于将容器连接到待引入容器的流体源的端口流体连通。 容器可以非常小，例如，体积小于约2毫升，并且流体通道可以具有小于容器体积的1.5％的通道体积。 根据另一方面，端口容积和通道容积的组合体积可以小于容器体积的约10％。 这种构造可以增加到达容器的添加流体的百分比。 在补料分批操作中，可以通过相同的通道添加和除去物质，使得可以在反应器内保持气体顶部空间。

公开(公告)号：US20070015276A1

公开(公告)日：2007-01-18

申请号：US11474050

申请日：2006-06-23

Applicant: Sean LeBlanc ,  Ian MacGregor ,  Bernardo Aumond ,  George Vella ,  A. Russo ,  Seth Rodgers ,  Andrey Zarur

Inventor： Sean LeBlanc ,  Ian MacGregor ,  Bernardo Aumond ,  George Vella ,  A. Russo ,  Seth Rodgers ,  Andrey Zarur

IPC: C12M3/00 ,  A61M31/00

CPC classification number: B01L3/0275 ,  B01L2200/026 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2300/0681

Abstract: Fluid transfer devices described herein can include a body portion and a tip portion. A fluid pathway extends through the body and tip portions through which fluid may be transferred, for example, from a fluid-dispensing apparatus to a fluidic chamber of a microreactor. In some embodiments, the fluid transfer device is connected to the fluid-dispensing apparatus with an engaging element. The engaging element may be part of the body, and can enable the dispensing apparatus to repeatedly engage the body at one predetermined position. The body is capable of storing the fluid received from the dispensing apparatus. The tip portion may be formed of a rigid material (e.g., a metal), and/or may be configured to repeatedly pierce a septum without damaging either the tip or the body. Advantageously, in certain embodiments, the fluid transfer device can controllably transfer small volumes of fluid (e.g., 1 μL) with a high degree of accuracy.

Abstract translation: 本文所述的流体输送装置可以包括主体部分和尖端部分。 流体通道延伸穿过主体和尖端部分，流体可通过该尖端部分例如从流体分配装置传送到微反应器的流体室。 在一些实施例中，流体输送装置用接合元件连接到流体分配装置。 接合元件可以是主体的一部分，并且能够使得分配装置能够在一个预定位置反复地接合身体。 身体能够存储从分配装置接收的流体。 尖端部分可以由刚性材料（例如，金属）形成，和/或可以构造成反复刺穿隔膜而不损坏尖端或者身体。 有利地，在某些实施方案中，流体转移装置可以高度准确地可控地传递小体积的流体（例如，1μl）。

公开(公告)号：US06905657B2

公开(公告)日：2005-06-14

申请号：US09827570

申请日：2001-04-05

Applicant: Allyn Hubbard ,  Samesh Kale ,  Scott Rollins ,  Jeremy P. Springhorn ,  Stephen P. Squinto ,  Prasad R. Akkapeddi

Inventor： Allyn Hubbard ,  Samesh Kale ,  Scott Rollins ,  Jeremy P. Springhorn ,  Stephen P. Squinto ,  Prasad R. Akkapeddi

IPC: B01J19/00 ,  B81B3/00 ,  C40B60/14 ,  C40B70/00 ,  G01N35/10 ,  B01L3/02 ,  B65B1/04 ,  G01N1/10

CPC classification number: G01N35/1074 ,  B01J19/0046 ,  B01J2219/00369 ,  B01J2219/00378 ,  B01J2219/00547 ,  C40B60/14 ,  C40B70/00 ,  G01N2035/1039 ,  Y10T436/2575

Abstract: In a liquid handling system including a liquid handling substrate having a plurality of channels for conducting a liquid sample in said substrate, where the channels terminate in a plurality of exit ports in an outer surface of the substrate for transfer of a quantity of the liquid sample. The handling system also includes a liquid storage and dispensing substrate having a plurality of separable cartridges corresponding to the channels, with each cartridge terminating at a microelectro mechanical system (MEMS) comprising a laminate of glass, silicon and a piezoelectric substance. The handling system further includes a liquid detecting system comprising a light emitting diode and a photo-detector, where each channel includes a reservoir in communication with a corresponding cartridge creating an interface therebetween. The handling system enables a method for storing and dispensing liquids including drawing a liquid sample into the channels either by capillary action, vacuum, electrostatic flow, a minipump or any combination thereof, storing the liquid sample into the cartridge, and dispensing the liquid sample.

Abstract translation: 在液体处理系统中，该液体处理系统包括液体处理基板，该液体处理基板具有用于在所述基板中传导液体样品的多个通道，其中所述通道终止于所述基板的外表面中的多个出口，用于转移一定数量的液体样品 。 处理系统还包括具有对应于通道的多个可分离盒的液体存储和分配基板，每个盒终止于包括玻璃，硅和压电物质的层叠体的微电子机械系统（MEMS）。 处理系统还包括液体检测系统，其包括发光二极管和光检测器，其中每个通道包括与相应的墨盒连通的储存器，其在其间形成界面。 处理系统实现了一种用于存储和分配液体的方法，包括通过毛细管作用，真空，静电流，微型泵或其任何组合将液体样品吸入通道，将液体样品储存在盒中并分配液体样品。

公开(公告)号：US07632675B2

公开(公告)日：2009-12-15

申请号：US10863584

申请日：2004-06-07

Applicant: Andrey J. Zarur ,  Seth T. Rodgers ,  Todd A. Basque ,  Ian K. MacGregor ,  Timothy J. Johnson

Inventor： Andrey J. Zarur ,  Seth T. Rodgers ,  Todd A. Basque ,  Ian K. MacGregor ,  Timothy J. Johnson

IPC: C12M1/02 ,  C12M3/02

CPC classification number: C12M23/08 ,  B01F9/0001 ,  B01F9/002 ,  B01F11/00 ,  B01L7/00 ,  B01L9/52 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0829 ,  B01L2400/0409 ,  C12M23/10 ,  C12M23/48 ,  C12M27/10 ,  C12M41/14 ,  Y10S435/809

Abstract: This disclosure generally relates to systems and methods for manipulating chambers and other substrates for chemical, biological, or biochemical samples, such as cell culture and other chambers, within units such as incubators. In certain embodiments, the invention provides a technique for maintaining a plurality of substrates or chambers in a housing within which a predetermined environment is maintained, and moving substrates or chambers in and out of the housing, in some cases without creating a large opening in the housing (e.g., by opening a door significantly larger than the substrates). A technique is provided, in certain embodiments, in which a plurality of substrates are mounted in fixed, secured relation to each other within a housing providing a predetermined, controlled environment, and are moved within the housing so that they can be evenly exposed to any differences in environment within the housing. In still another embodiment, the invention provides a method for rotating a substrate or chamber about a substantially vertical and/or horizontal axis.

Abstract translation: 本公开总体上涉及用于在诸如培养箱的单元内操作用于化学，生物或生化样品（例如细胞培养物和其它室）的室和其它底物的系统和方法。 在某些实施例中，本发明提供了一种用于在保持预定环境的壳体中保持多个基板或室的技术，以及将基板或室移入和移出壳体的方法，在某些情况下不会在 （例如，通过打开比基板大得多的门）。 在某些实施例中提供了一种技术，其中多个基板以固定的，固定的关系安装在提供预定的受控环境的壳体内，并且在壳体内移动，使得它们可以均匀地暴露于任何 房屋环境差异。 在另一个实施例中，本发明提供了一种用于围绕基本垂直和/或水平轴线旋转衬底或室的方法。

公开(公告)号：US20090275115A1

公开(公告)日：2009-11-05

申请号：US12347579

申请日：2008-12-31

Applicant: Andrey Zarur Jury ,  Mark D. Angelino

Inventor： Andrey Zarur Jury ,  Mark D. Angelino

IPC: C12M3/00

CPC classification number: B01F13/0064 ,  B01F5/0256 ,  B01F5/061 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0094 ,  B01F15/00935 ,  B01F2005/0621 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/00835 ,  B01J2219/00862 ,  B01J2219/00871 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00907 ,  B01J2219/00957 ,  B01J2219/00961 ,  B01J2219/00963 ,  B01J2219/00966 ,  C12M23/16

Abstract: Chemical and biological reactors, including microreactors, are provided. Exemplary reactors include a plurality of reactors operable in parallel, where each reactor has a small volume and, together, the reactors produce a large volume of product. Reaction systems can include mixing chambers, heating/dispersion units, reaction chambers, and separation units. Components of the reactors can be readily formed from a variety of materials. For example, they can be etched from silicon. Components are connectable to and separable from each other to form a variety of types of reactors, and the reactors can be attachable to and separable from each other to add significant flexibility in parallel and/or series reactor operation.

Abstract translation: 提供化学和生物反应堆，包括微反应器。 示例性反应器包括可并行操作的多个反应器，其中每个反应器具有小体积，并且一起反应器产生大体积的产物。 反应系统可以包括混合室，加热/分散单元，反应室和分离单元。 反应器的组分可以容易地由各种材料形成。 例如，它们可以从硅上蚀刻。 组件可彼此连接并可分离以形成各种类型的反应器，并且反应器可以彼此附接和分离，以在并联和/或串联反应器操作中增加显着的灵活性。

公开(公告)号：US07485454B1

公开(公告)日：2009-02-03

申请号：US09707852

申请日：2000-11-07

Applicant: Andrey Zarur Jury ,  Mark D. Angelino

Inventor： Andrey Zarur Jury ,  Mark D. Angelino

IPC: C12M1/12

CPC classification number: B01F13/0064 ,  B01F5/0256 ,  B01F5/061 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0094 ,  B01F15/00935 ,  B01F2005/0621 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/00835 ,  B01J2219/00862 ,  B01J2219/00871 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00907 ,  B01J2219/00957 ,  B01J2219/00961 ,  B01J2219/00963 ,  B01J2219/00966 ,  C12M23/16

Abstract: Chemical and biological reactors, including microreactors, are provided. Exemplary reactors include a plurality of reactors operable in parallel, where each reactor has a small volume and, together, the reactors produce a large volume of product. Reaction systems can include mixing chambers, heating/dispersion units, reaction chambers, and separation units. Components of the reactors can be readily formed from a variety of materials. For example, they can be etched from silicon. Components are connectable to and separable from each other to form a variety of types of reactors, and the reactors can be attachable to and separable from each other to add significant flexibility in parallel and/or series reactor operation.

Abstract translation: 提供化学和生物反应堆，包括微反应器。 示例性反应器包括可并行操作的多个反应器，其中每个反应器具有小体积，并且一起反应器产生大体积的产物。 反应系统可以包括混合室，加热/分散单元，反应室和分离单元。 反应器的组分可以容易地由各种材料形成。 例如，它们可以从硅上蚀刻。 组件可彼此连接并可分离以形成各种类型的反应器，并且反应器可以彼此附接和分离，以在并联和/或串联反应器操作中增加显着的灵活性。

公开(公告)号：US20070099292A1

公开(公告)日：2007-05-03

申请号：US11584343

申请日：2006-10-20

Applicant: Scott Miller ,  Sean LeBlanc ,  Seth Rodgers ,  Andrey Zarur

Inventor： Scott Miller ,  Sean LeBlanc ,  Seth Rodgers ,  Andrey Zarur

IPC: C12M1/34

CPC classification number: B01L3/502715 ,  B01L2200/025 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2400/046 ,  G01N2021/0346

Abstract: Various aspects of the present invention relate to light-interacting components suitable for use in chips and other reactor systems. These components may include waveguides, optical fibers, light sources, photodetectors, optical elements, and the like. If waveguides are used, they may be fashioned out of any material able to transmit light to or from the reaction site. The chip may contain a reaction site having a volume of less than about 1 ml. In some embodiments, the chip may be constructed in such a way as to be able to support a living cell. The chip may be used for imaging or analysis, or the chip may be used to facilitate a chemical or biological reaction, which may be light-sensitive or light-activated in certain cases. Other facilitated reactions may include the production or consumption of a chemical or biological species. In some embodiments, the chip may include more than one component or component type, or more than one reaction site.

Abstract translation: 本发明的各个方面涉及适用于芯片和其它反应器系统的光相互作用组件。 这些组件可以包括波导，光纤，光源，光电检测器，光学元件等。 如果使用波导，则它们可以从能够将光透射到反应位点的任何材料形成。 芯片可以含有体积小于约1ml的反应位点。 在一些实施例中，芯片可以以能够支撑活细胞的方式构造。 该芯片可以用于成像或分析，或者芯片可以用于促进在某些情况下可能是光敏感或光激活的化学或生物反应。 其他促进反应可能包括化学或生物物种的生产或消费。 在一些实施方案中，芯片可以包括多于一种组分或组分类型，或多于一个反应位点。

公开(公告)号：US20070037278A1

公开(公告)日：2007-02-15

申请号：US11584037

申请日：2006-10-20

Applicant: Seth Rodgers ,  A. Russo ,  Howard Schreyer ,  Andrey Jury

Inventor： Seth Rodgers ,  A. Russo ,  Howard Schreyer ,  Andrey Jury

IPC: C12M1/12

CPC classification number: C12M25/02 ,  C12M23/12 ,  C12M23/24 ,  C12M29/04 ,  C12M41/34

Abstract: The present invention is directed to materials and reactor systems having humidity and/or gas control. The material may have high oxygen permeability and/or low water vapor permeability. In some cases, the material may have sufficient permeance and/or permeability to allow cell culture to occur in a chip or other reactor system using the material. In certain embodiments, the material may be positioned adjacent to or abut a reaction site within a chip or reactor; in other embodiments, the material may be positioned such that it is in fluidic communication with the reaction site. The material may also be porous and/or transparent in some cases. In one set of embodiments, the material include a polymer that is branched, and/or contains bulky side groups that allow the polymer to have a more open structure. In some cases, the material may include two or more layers. Each layer may have a desired property, which may include, for example, permeability, transparency, cytophilicity, biophilicity, hydrophilicity, or a structural feature. In some embodiments, the material may be chosen so as to promote cell growth within the chip or reactor.