-
![IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES](/abs-image/US/2010/04/01/US20100081154A1/abs.jpg.150x150.jpg)
11.发明申请IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES 审中-公开细菌[2Fe-2S]二羟基脱氢酶的鉴定和应用公开(公告)号：US20100081154A1
公开(公告)日：2010-04-01
申请号：US12569636
申请日：2009-09-29
CPC分类号： G16B30/00 , C12N9/88 , C12P7/16 , C12P7/40 , C12P13/06 , C12P13/08 , C12Y402/01009 , Y02E50/10
摘要： A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.
摘要翻译： 发现一组具有[2Fe-2S]簇的细菌二羟基酸脱水酶。 细菌[2Fe-2S] DHADs在细菌和酵母细胞中表达为异源蛋白质，提供DHAD活性，用于将2,3-二羟基异戊酸酯转化为α-酮异戊酸酯或2,3-二羟甲基戊酸酯转化成α-酮戊二酸酯。 异丁醇和其他化合物可以在包括细菌[2Fe-2S] DHAD活性的途径中合成。
-
12.发明申请公开(公告)号：US20100022018A1
公开(公告)日：2010-01-28
申请号：US12446997
申请日：2007-11-28
申请人： Dennis Flint
发明人： Dennis Flint
CPC分类号： C12Q1/37 , G01N33/5308 , G01N33/84 , Y10T436/182
摘要： The invention relates to a method for detecting compounds containing a free sulfhydryl group in a sample and a test device which is used to detect such compounds. The method and test device use a papain-based assay where the papain is initially complexed with a metal ion making it inactive. Sulfydryl compounds in a sample activate the papain. Enzymatic activity of papain on a papain substrate releases a dye producing a detectable signal. The test device may be used in detecting compounds containing a free sulfhydryl group in industrial, environmental, experimental and biological samples.
摘要翻译： 本发明涉及用于检测样品中含有游离巯基的化合物的方法和用于检测这些化合物的测试装置。 该方法和测试装置使用基于木瓜蛋白酶的测定法，其中木瓜蛋白酶最初与金属离子复合使其失活。 样品中的Sulfydryl化合物激活木瓜蛋白酶。 木瓜蛋白酶在木瓜蛋白酶底物上的酶活性释放产生可检测信号的染料。 测试装置可用于检测工业，环境，实验和生物样品中含有游离巯基的化合物。
-
公开(公告)号：US07273737B2
公开(公告)日：2007-09-25
申请号：US10700171
申请日：2003-11-03
CPC分类号： C12N9/1085 , C12N15/8243 , C12N15/8279
摘要： This invention pertains to nucleic acid fragments encoding plant proteins that are homologs to the cis-prenyltransferases UPP synthase from the bacterium Micrococcus luteus or Dedol-PP synthase from yeast Saccharomyces cerevisiae. More specifically, this invention pertains to cis-prenyltransferase homologs from wheat, grape, soybean, rice, African daisy, rubber tree latex and pot marigold.
摘要翻译： 本发明涉及编码植物蛋白质的核酸片段，其与来自酵母酿酒酵母的细菌微球菌或Dedol-PP合酶的顺式 - 异戊烯基转移酶UPP合酶的同系物。 更具体地说，本发明涉及来自小麦，葡萄，大豆，大米，非洲雏菊，橡胶树乳胶和万寿菊的顺式 - 异戊烯基转移酶同源物。
-
公开(公告)号：US07217810B2
公开(公告)日：2007-05-15
申请号：US11336680
申请日：2006-01-20
申请人： Meyer Knut , Dennis Flint , Paul V. Vitanen
发明人： Meyer Knut , Dennis Flint , Paul V. Vitanen
CPC分类号： C12N15/8257
摘要： This invention relates to methods and materials to produce hydroquinone glucoside in genetically modified green plants and microorganisms.
摘要翻译： 本发明涉及在遗传修饰的绿色植物和微生物中产生氢醌葡糖苷的方法和材料。
-
公开(公告)号：US06645747B1
公开(公告)日：2003-11-11
申请号：US09655908
申请日：2000-09-06
IPC分类号： C12N912
CPC分类号： C12N9/1085 , C12N15/8243 , C12N15/8279
摘要： This invention pertains to nucleic acid fragments encoding plant proteins that are homologs to the cis-prenyltransferases UPP synthase from the bacterium Micrococcus luteus or Dedol-PP synthase from yeast Saccharomyces cerevisiae. More specifically, this invention pertains to cis-prenyltransferase homologs from wheat, grape, soybean, rice, African daisy, rubber tree latex and pot marigold.
摘要翻译： 本发明涉及编码植物蛋白质的核酸片段，其与来自酵母酿酒酵母的细菌微球菌或Dedol-PP合酶的顺式 - 异戊烯基转移酶UPP合酶的同系物。 更具体地说，本发明涉及来自小麦，葡萄，大豆，大米，非洲雏菊，橡胶树乳胶和万寿菊的顺式 - 异戊烯基转移酶同源物。
-
公开(公告)号：US08951774B2
公开(公告)日：2015-02-10
申请号：US12939315
申请日：2010-11-04
IPC分类号： C12P21/06 , C12P7/04 , C12N1/20 , C12N1/00 , C12N15/00 , C07H21/04 , C12N15/81 , C12N9/04 , C12N9/02 , C12N9/06 , C12N9/10 , C12N9/88 , C12N15/52 , C12P7/16 , C12N15/70 , C12N15/75 , C12N15/74
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0016 , C12N9/1022 , C12N9/1096 , C12N9/88 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/746 , C12N15/75 , C12N15/81 , C12Y101/01001 , C12Y101/01002 , C12Y101/01086 , C12Y102/00 , C12Y102/01003 , C12Y102/04004 , C12Y202/01006 , C12Y401/01072 , C12Y402/01009 , Y02E50/10 , Y02E50/343 , Y02P20/52
摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达异丁醇生物合成途径的重组细菌的发酵生长来生产丁醇，优选异丁醇。
-
公开(公告)号：US20110301388A1
公开(公告)日：2011-12-08
申请号：US13205351
申请日：2011-08-08
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0016 , C12N9/1022 , C12N9/1096 , C12N9/88 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/746 , C12N15/75 , C12N15/81 , C12Y101/01001 , C12Y101/01002 , C12Y101/01086 , C12Y102/00 , C12Y102/01003 , C12Y102/04004 , C12Y202/01006 , C12Y401/01072 , C12Y402/01009 , Y02E50/10 , Y02E50/343 , Y02P20/52
摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
-
公开(公告)号：US20110195505A1
公开(公告)日：2011-08-11
申请号：US12899760
申请日：2010-10-07
申请人： LORI JEAN EULER , DENNIS FLINT , BRIAN JAMES PAUL , TINA K. VAN DYK , RICK W. YE
发明人： LORI JEAN EULER , DENNIS FLINT , BRIAN JAMES PAUL , TINA K. VAN DYK , RICK W. YE
CPC分类号： C12P7/16 , C07K14/335 , C12N1/36 , Y02E50/10
摘要： Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the saturated fatty acids content in bacterial cell membrane was increased. Methods for increasing the concentration of saturated fatty acids in the membranes of bacteria that are not natural butanol produces are described whereby tolerance of the bacterial cell to butanol is increased. Saturated fatty acids concentration in the bacterial cell membrane increased upon exogenously feeding saturated fatty acids to cells. Bacterial strains useful for production of butanol are described herein having modified unsaturated fatty acid biosynthetic pathway.
摘要翻译： 当细菌细胞膜中的饱和脂肪酸含量增加时，发现不是天然丁醇生产者的细菌对丁醇具有增加的耐受性。 描述了增加非天然丁醇产生的细菌膜中饱和脂肪酸浓度的方法，其中细菌细胞对丁醇的耐受性增加。 饱和脂肪酸在细胞细胞膜中的浓度在将饱和脂肪酸外源供给细胞后增加。 本文描述了可用于生产丁醇的细菌菌株，其具有改性的不饱和脂肪酸生物合成途径。
-
公开(公告)号：US08426174B2
公开(公告)日：2013-04-23
申请号：US12110510
申请日：2008-04-28
申请人： Michael G. Bramucci , Dennis Flint , Edward S. Miller, Jr. , Vasantha Nagarajan , Natalia Sedkova , Manjari Singh , Tina K. Van Dyk
发明人： Michael G. Bramucci , Dennis Flint , Edward S. Miller, Jr. , Vasantha Nagarajan , Natalia Sedkova , Manjari Singh , Tina K. Van Dyk
IPC分类号： C12P7/16
摘要： A method for the production of 2-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.
摘要翻译： 公开了通过使用微生物生产宿主发酵生产2-丁醇的方法。 该方法在发酵过程中使用温度降低，导致生产主体对丁醇产物的更强的耐受性。
-
公开(公告)号：US08273558B2
公开(公告)日：2012-09-25
申请号：US12966333
申请日：2010-12-13
摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达异丁醇生物合成途径的重组细菌的发酵生长来生产丁醇，优选异丁醇。
-
-
-
-
-
-
-
-
-
搜索结果
37 条记录 (耗时 0.041 秒)
