公开(公告)号：US20170296155A1

公开(公告)日：2017-10-19

申请号：US15515980

申请日：2015-09-30

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Johan SKOG ,  Bill TISEL ,  Joseph BAKER

IPC: A61B10/00 ,  A61B90/90 ,  B01L3/00

CPC classification number: A61B10/007 ,  A61B90/90 ,  B01L3/50825 ,  B01L2200/0689 ,  B01L2200/087 ,  B01L2200/14 ,  B01L2200/141 ,  B01L2300/021 ,  B01L2300/042 ,  B01L2300/0832 ,  B01L2300/0851 ,  B01L2300/087

Abstract: In order to address an inability to separate portions of a sample and/or substance, in some embodiments, methods and apparatuses for separating components and/or portions of a sample are provided. For example, a sample collection container may comprise two areas within the container, one which is configured to contain a first volume of the sample, and one which is configured to contain a second volume of the sample. A float device may be configured to seal the portion of the container collecting the first volume from the portion of the container collecting the second volume, after the former portion of the container has been filled. The sample collection container may further comprise a top cap configured to further seal the two portions of the container, and to prevent leakage and/or spilling of the sample from the sample collection container.

公开(公告)号：US20160237422A1

公开(公告)日：2016-08-18

申请号：US15138746

申请日：2016-04-26

Applicant: Exosome Diagnostics, Inc.

Inventor： Wayne Comper ,  Leileata M. Russo ,  Johan Karl Olov Skog

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  C07H1/08 ,  C07H21/02 ,  C12N15/1006 ,  C12N15/101 ,  C12Q1/6806 ,  C12Q2527/127 ,  C12Q2563/161

Abstract: Methods for extracting high quality nucleic acids from a heterogenous collection of nucleic acid-containing materials from a biological sample are disclosed. The heterogenous collection of nucleic-acid containing materials may contain cells or microvesicles, or both. The extractions obtained by the methods described herein are characterized by high yield and high integrity, making the extracted nucleic acids useful for various applications in which high quality nucleic acid extractions are preferred, e.g., a diagnosis, prognosis, or therapy evaluation for a medical condition.

Abstract translation: 公开了从生物样品的含有核酸的材料的异质收集中提取高质量核酸的方法。 含有核酸的材料的异质收集可能含有细胞或微泡，或两者。 通过本文描述的方法获得的提取物的特征在于高产率和高完整性，使得提取的核酸可用于优先选择高质量核酸提取的各种应用，例如用于医疗状况的诊断，预后或治疗评估 。

公开(公告)号：US20160075788A1

公开(公告)日：2016-03-17

申请号：US14768275

申请日：2014-02-14

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Johan Karl Olov Skog ,  Emily Berghoff ,  Lori LoGuidice

IPC: C07K16/28 ,  G01N33/574 ,  C12N15/113 ,  C12Q1/68 ,  C07K14/71 ,  A61K47/48

CPC classification number: C07K16/2863 ,  A61K47/6849 ,  C07K14/485 ,  C07K14/71 ,  C07K2317/76 ,  C12N15/1138 ,  C12N2310/14 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156 ,  G01N33/57492 ,  G01N2333/71 ,  G01N2800/52

Abstract: The present invention features a novel EGFR variant, EGFRvVI, and methods for detecting the novel EGFR variant. The novel EGFR variant is preferentially expressed in some cancers. Methods for detecting EGFRvVI may aid in the diagnosis, prognosis, and therapeutic assessment of a subject.

Abstract translation: 本发明的特征在于一种新的EGFR变体，EGFRvVI和用于检测新型EGFR变体的方法。 新型EGFR变体优先在一些癌症中表达。 检测EGFRvVI的方法可能有助于对受试者的诊断，预后和治疗评估。

公开(公告)号：US20150252428A1

公开(公告)日：2015-09-10

申请号：US14433460

申请日：2013-10-03

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Wayne Comper ,  Aparna Ramachandran ,  Haoheng Yan ,  Leileata M. Russo ,  Johan Karl Olav Skog

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The present invention discloses methods for diagnosing or prognosing a disease or medical condition in a subject by detecting the presence or absence of BRAF mutant nucleic acids from nucleic acids extracted from microvesicles from a biological sample. The present invention also discloses methods for assessing the responsiveness or determining a treatment regimen for a subject in need thereof by detecting the presence or absence of BRAF mutant nucleic acids from nucleic acids extracted from microvesicles from a biological sample. Methods for isolating microvesicles and extracting DNA and/or RNA from the microvesicles are also described.

Abstract translation: 本发明公开了通过从生物样品的微泡提取的核酸中检测BRAF突变核酸的存在或不存在来诊断或预测受试者的疾病或医学病症的方法。 本发明还公开了通过从生物样品的微泡提取的核酸中检测BRAF突变核酸的存在或不存在来评价对有需要的受试者的反应性或确定治疗方案的方法。 还描述了分离微泡并从微泡中提取DNA和/或RNA的方法。

公开(公告)号：US20250075258A1

公开(公告)日：2025-03-06

申请号：US18579636

申请日：2022-07-18

Applicant: Exosome Diagnostics, Inc. ,  Trustees of Tufts College

Inventor： Sudipto Kumar CHAKRABORTTY ,  Steven LANG ,  Yevgenia Leona Khodor TOLAN ,  Martina RAUSCHER ,  Vasisht TADIGOTLA ,  Wei YU ,  Athena S. PAPAS ,  Johan Karl Olov SKOG ,  Brian C. HAYNES ,  Shuran XING

IPC: C12Q1/6832 ,  C12Q1/6869

Abstract: The present disclosure is directed to methods of using salivary exosomes to detect and treat Sjögren's syndrome in a subject.

公开(公告)号：US20230203587A1

公开(公告)日：2023-06-29

申请号：US17928452

申请日：2021-05-28

Applicant: Exosome Diagnostics, Inc. ,  The Brigham and Women's Hospital, Inc.

Inventor： Johan SKOG ,  Vasisht TADIGOTLA ,  James HURLEY ,  Jamil AZZI

IPC: C12Q1/6883 ,  C12Q1/686

CPC classification number: C12Q1/6883 ,  C12Q1/686 ,  C12Q2600/118 ,  C12Q2600/158

Abstract: The present disclosure relates to methods of identifying and treating kidney rejection in a subject comprising analyzing microvesicular RNA, cell-free DNA or the combination of microvesicular and cell-free DNA.”

公开(公告)号：US11214833B2

公开(公告)日：2022-01-04

申请号：US16098500

申请日：2017-05-05

Applicant: Exosome Diagnostics, Inc. ,  The Brigham and Women's Hospital, Inc.

Inventor： Johan Karl Olov Skog ,  Jamil Azzi

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6876 ,  C12Q1/686

Abstract: The invention relates generally to the use of microvesicle RNA signatures for diagnosis, predicting, and/or to monitor treatment efficacy, including patients who are candidates for renal transplant and/or who have received a renal transplant.

公开(公告)号：US20210403881A1

公开(公告)日：2021-12-30

申请号：US17295287

申请日：2019-11-20

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg STOLL ,  Daniel ENDERLE ,  Mikkel NOERHOLM ,  Johan Karl Olov SKOG

IPC: C12N7/00 ,  C12Q1/70 ,  C12N15/10

Abstract: The present disclosure provides compositions, methods and kits for internal controls of microvesicle isolations. The compositions, methods and kits can comprise enveloped viruses, including, but not limited to, inactive mouse hepatitis virus (MHV).

公开(公告)号：US20210388453A1

公开(公告)日：2021-12-16

申请号：US17411606

申请日：2021-08-25

Applicant: Exosome Diagnostics, Inc.

Inventor： Mikkel NOERHOLM ,  Susan BELZER ,  Charlotte ROMAIN ,  Johan Karl Olov SKOG ,  Leileata M. RUSSO ,  Wayne COMPER

IPC: C12Q1/6886 ,  C12N7/00

Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.

公开(公告)号：US11136627B2

公开(公告)日：2021-10-05

申请号：US14424694

申请日：2013-08-30

Applicant: Exosome Diagnostics, Inc.

Inventor： Mikkel Noerholm ,  Susan Belzer ,  Charlotte Romain ,  Johan Karl Olov Skog ,  Leileata M. Russo ,  Wayne Comper

IPC: C12P19/34 ,  C12Q1/6886 ,  C12N7/00

Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.