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    Process for the depletion or removal of endotoxins
    12.
    发明授权
    Process for the depletion or removal of endotoxins 失效
    内毒素消耗或去除的过程

    公开(公告)号：US5747663A

    公开(公告)日：1998-05-05

    申请号：US687522

    申请日：1996-09-30

    Applicant: Metin Colpan ,  Peter Moritz ,  Joachim Schorr

    Inventor： Metin Colpan ,  Peter Moritz ,  Joachim Schorr

    IPC: B01J20/281 ,  A61K31/70 ,  A61K31/7088 ,  A61K48/00 ,  A61P35/00 ,  A61P43/00 ,  B01J20/26 ,  B01J41/04 ,  C07H1/08 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/10 ,  C12N15/87 ,  G01N30/88 ,  C12P19/34 ,  C12N1/06

    CPC classification number: B01D15/363 ,  B01D15/3804 ,  C12N15/101 ,  C12N15/87 ,  Y10S435/81 ,  Y10S435/82 ,  Y10S436/808 ,  Y10T436/10

    Abstract: A process is disclosed for reducing or removing endotoxins from compositions containing therapeutic active substances extracted from natural sources by genetic engineering and/or biotechnology. For that purpose, the compositions are treated with chromatographic materials. The natural sources are disintegrated, the thus obtained fractions are, if required, centrifuged, filtered or treated using affinity chromatography methods, the fractions are pre-incubated in an aqueous salt solution and detergents, are treated with anion exchange materials, then washed with another salt solution. The active substances are eluted from the anion exchanger then further purified in a manner known per se.

    Abstract translation: PCT No.PCT / EP95 / 00391 Sec。 371日期1996年9月30日 102（e）1996年9月30日PCT提交1995年2月3日PCT公布。 WO95 / 21179 PCT公开号 日期1995年8月10日公开了一种通过基因工程和/或生物技术从含有从天然来源提取的治疗活性物质的组合物中还原或去除内毒素的方法。 为此，用色谱材料处理组合物。 将天然来源分解，如果需要，如此获得的级分用亲和层析方法离心，过滤或处理，将级分在盐水溶液和洗涤剂中预温育，用阴离子交换材料处理，然后用另一种 盐溶液。 活性物质从阴离子交换剂洗脱，然后以本身已知的方式进一步纯化。

    Device and a method for separating liquid samples
    14.
    发明授权
    Device and a method for separating liquid samples 失效
    装置和分离液体样品的方法

    公开(公告)号：US5264184A

    公开(公告)日：1993-11-23

    申请号：US671448

    申请日：1991-03-19

    Applicant: James E. Aysta ,  Metin Colpan

    Inventor： James E. Aysta ,  Metin Colpan

    IPC: B01D15/00 ,  B01D24/00 ,  B01D29/01 ,  B01D61/18 ,  B01L3/00 ,  B01L99/00 ,  C12M1/00 ,  C12Q1/68 ,  B01L11/00 ,  B01D29/00

    CPC classification number: B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L3/50855 ,  Y10T436/25375 ,  Y10T436/255

    Abstract: A device (10) for separating liquid samples has a sample container (12), bottom wall (20) of which has an outlet opening (22) provided therein. The outlet opening (22) is joined by an outlet spout (24) extending in the axial direction of the sample container (12). On bottom wall (20) there is a separation layer (28). Below sample container (12), a collecting container (32) is arranged abutting sample container (12) such that an exchange of air between the interior of the collecting chamber (32) and the environment is possible, yet an escape of liquid is largely inhibited. The contact surface between collecting chamber (32) and sample container (12) and end (26) of the outlet spout (24) through which the liquid is discharged, are axially spaced apart.

    Abstract translation: 用于分离液体样品的装置（10）具有样品容器（12），其底壁（20）具有设置在其中的出口（22）。 出口开口（22）通过在样品容器（12）的轴向方向上延伸的出口喷口（24）连接。 在底壁（20）上有分离层（28）。 在样本容器（12）的下面，收集容器（32）设置成邻接样本容器（12），使得可以在收集室（32）的内部和环境之间交换空气，但是液体逸出很大程度上 抑制。 收集室（32）和样品容器（12）之间的接触表面以及排出液体的出口喷口（24）的端部（26）轴向间隔开。

    Device and a process for the isolation of nucleic acids
    17.
    发明授权
    Device and a process for the isolation of nucleic acids 失效
    装置和分离核酸的方法

    公开(公告)号：US08247545B1

    公开(公告)日：2012-08-21

    申请号：US08796040

    申请日：1997-02-05

    Applicant: Metin Colpan

    Inventor： Metin Colpan

    IPC: C07H21/00

    CPC classification number: C12N15/101 ,  B01D39/00 ,  C12N15/1017 ,  Y10T436/25375 ,  Y10T436/255

    Abstract: There is described is a process for the isolation and purification of nucleic acids such as plasmid or genomic DNA from cells or other sources, wherein a) the cells containing nucleic acids are digested and cell debris is removed, or other samples containing nucleic acids are treated with anion exchangers, namely, in buffer solutions of low ionic strength, b) thereafter, the nucleic acids are desorbed from the anion exchanger using a buffer of high ionic strength, in order to be subsequently c) treated in said buffer of high ionic strength or in the presence of lower alcohols and/or poly(ethylene glycol) with a mineral support material, with adsorption of the nucleic acid to the surface of the mineral support materials, whereupon d) desorption of the nucleic acid is effected using water or a buffer solution of low ionic strength. The device for operating the inventive process consists of a hollow body (1) with an inlet opening (7) and an outlet opening (8), wherein in the hollow body (1), between two securing means (5, 6), a powdered first material based on silica gel (10) is arranged, and a second material (11) is placed between the first material (10) and the outlet opening (8), the first and second materials (10, 11) having different adsorption characteristics for nucleic acids.

    Abstract translation: 描述了用于分离和纯化来自细胞或其他来源的核酸如质粒或基因组DNA的方法，其中a）将含有核酸的细胞消化并除去细胞碎片，或处理含有核酸的其它样品 具有阴离子交换剂，即在低离子强度的缓冲溶液中，b）此后，使用高离子强度的缓冲液将核酸从阴离子交换剂中解吸，以便随后c）在所述高离子强度缓冲液中处理 或在具有矿物载体材料的低级醇和/或聚（乙二醇）的存在下，将核酸吸附到矿物载体材料的表面，因此d）核酸的解吸是使用水或 缓冲溶液的低离子强度。 用于操作本发明方法的装置包括具有入口开口（7）和出口开口（8）的空心体（1），其中在中空体（1）中，在两个固定装置（5,6）之间， 布置基于硅胶（10）的粉末状第一材料，并且第二材料（11）被放置在第一材料（10）和出口（8）之间，第一和第二材料（10,11）具有不同的吸附 核酸的特征。

    Device and process for isolating nucleic acids from cell suspension
    19.
    发明授权
    Device and process for isolating nucleic acids from cell suspension 失效
    从细胞悬浮液中分离核酸的装置和方法

    公开(公告)号：US5652141A

    公开(公告)日：1997-07-29

    申请号：US442100

    申请日：1995-05-16

    Applicant: Karsten Henco ,  Metin Colpan ,  Petra Feuser

    Inventor： Karsten Henco ,  Metin Colpan ,  Petra Feuser

    IPC: C12N15/10 ,  C12Q1/68 ,  C12N1/08 ,  C07H1/06

    CPC classification number: C12N15/101 ,  C12N15/1006 ,  C12Q1/6806

    Abstract: A device and a process for isolating nucleic acids by lysing intact cells and removing nucleic acids emerging from the lysed cells by the following steps:a) the cells are immobilized in a porous matrix, with the size of matrix voids being in the range of the type of cell to be lysed;b) the cells are lysed;c) the nucleic acids are fixated on the matrix surface, and subsequentlyd) are eluted.

    Abstract translation: 通过以下步骤裂解完整细胞和从裂解的细胞中除去出现的核酸来分离核酸的装置和方法：a）将细胞固定在多孔基质中，其中基质空隙的大小在 待裂解的细胞类型; b）细胞裂解; c）将核酸固定在基质表面上，随后d）被洗脱。

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