公开(公告)号：US20130273013A1

公开(公告)日：2013-10-17

申请号：US13989805

申请日：2011-12-15

Applicant: Michel Revel ,  Judith Chebath ,  Guy Slutsky ,  Alon Levy ,  Michal Izrael ,  Arik Hasson ,  Molakandov Kfir ,  Rosalia Kaufman

Inventor： Michel Revel ,  Judith Chebath ,  Guy Slutsky ,  Alon Levy ,  Michal Izrael ,  Arik Hasson ,  Molakandov Kfir ,  Rosalia Kaufman

IPC: C12N5/071

CPC classification number: C12N5/0676 ,  C12N5/0677 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2501/01 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/58 ,  C12N2501/60 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2533/54 ,  C12N2533/74

Abstract: A method of generating islet cells from pluripotent stem cells is disclosed. The method comprises: (a) culturing the pluripotent stem cells in a differentiation medium so as to differentiate the pluripotent stem cells into endoderm cells; and (b) culturing the endoderm cells in a medium comprising at least one growth factor, a cAMP inducer and retinoic acid (RA), said at least one growth factor being selected from the group consisting of FGF10, bFGF and FGF7 so as to generate further differentiated cells; and (c) culturing the further differentiated cells in a medium comprising a maturation factor selected from the group consisting of nicotinamide, GLP-1 and exendin 4, thereby generating islet cells from pluripotent stem cells. Further methods of generating islet cells are also disclosed, isolated cell populations comprising same and uses thereof.

Abstract translation: 公开了从多能干细胞产生胰岛细胞的方法。 该方法包括：（a）在分化培养基中培养多能干细胞，以将多能干细胞分化成内胚层细胞; 和（b）在包含至少一种生长因子，cAMP诱导剂和视黄酸（RA）的培养基中培养所述内胚层细胞，所述至少一种生长因子选自FGF10，bFGF和FGF7，以产生 进一步分化细胞; 和（c）在包含选自烟酰胺，GLP-1和毒蜥外泌肽4的成熟因子的培养基中培养进一步分化的细胞，从而从多能干细胞产生胰岛细胞。 还公开了产生胰岛细胞的其它方法，包含其的分离的细胞群体及其用途。

公开(公告)号：US5902576A

公开(公告)日：1999-05-11

申请号：US430248

申请日：1995-04-28

Applicant: Lea Eisenbach ,  Angel Porgador ,  Michael Feldman ,  Michel Revel

Inventor： Lea Eisenbach ,  Angel Porgador ,  Michael Feldman ,  Michel Revel

IPC: A61K39/00 ,  A61K48/00 ,  C12N5/00

CPC classification number: A61K39/0011 ,  A61K2039/5152 ,  A61K2039/5156 ,  A61K48/00

Abstract: An anti-tumor pharmaceutical composition includes cells into which a gene encoding human IL-6 has been inserted. A method of treatment of a patient suffering from cancer to prevent and/or inhibit the development of metastases by administering to the patient the anti-tumor pharmaceutical composition including the above mentioned cells.

Abstract translation: 抗肿瘤药物组合物包括插入了编码人IL-6的基因的细胞。 一种通过向患者施用包含上述细胞的抗肿瘤药物组合物来治疗患有癌症的患者以预防和/或抑制转移发展的方法。

公开(公告)号：US5643749A

公开(公告)日：1997-07-01

申请号：US328256

申请日：1994-10-24

Applicant: Michel Revel ,  Carolina Abramovich ,  Edward Ratovitski

Inventor： Michel Revel ,  Carolina Abramovich ,  Edward Ratovitski

IPC: C12N15/09 ,  A61K38/00 ,  A61P1/16 ,  A61P25/00 ,  A61P31/12 ,  A61P35/00 ,  A61P35/02 ,  A61P37/02 ,  A61P37/06 ,  A61P43/00 ,  C07H21/04 ,  C07K1/22 ,  C07K14/52 ,  C07K14/555 ,  C07K14/56 ,  C07K14/565 ,  C07K14/715 ,  C07K16/00 ,  C07K19/00 ,  C12N15/12 ,  C12P21/02 ,  G01N33/68 ,  C12N15/63

CPC classification number: C07K14/715 ,  G01N33/6866 ,  A61K38/00 ,  C07K2319/00

Abstract: New forms of interferon .alpha.-receptors are provided. They may be prepared recombinantly and may be used in diagnosis and therapy.

Abstract translation: 提供了新形式的干扰素α受体。 它们可以重组制备，可用于诊断和治疗。

公开(公告)号：US5468609A

公开(公告)日：1995-11-21

申请号：US425933

申请日：1982-09-28

Applicant: Michel Revel ,  Pierre Tiollais

Inventor： Michel Revel ,  Pierre Tiollais

IPC: C12N15/09 ,  C07H21/02 ,  C07H21/04 ,  C07K14/54 ,  C07K14/565 ,  C12N15/00 ,  C12N15/22 ,  C12P19/34 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91 ,  C07K14/52

CPC classification number: C07K14/5412 ,  C07K14/565

Abstract: The present invention relates to a process to isolate genetic material (DNA) containing the nucleotide sequence coding for interferon in human fibroblastic cells which comprises cultivating cells producing interferon when exposed to an inducer of interferon, exposing same to such inducer, extracting messenger RNA from said induced cells, purifying the interferon messenger RNA, transcribing the messenger RNA into DNA and cloning the DNA in a suitable vector. Preferred cells are human diploid foreskin cells. The invention further relates to a process for engineering a bacterial strain to produce interferon polypeptide which comprises introducing a cloned interferon DNA into a suitable vector-carrier. A preferred vector-carrier is E. coli. The invention also relates to the mRNA of human interferon in highly purified form, to the mRNA of human interferon in .beta.1 highly purified form, to the mRNA of human interferon in .beta.2 highly purified form, to the DNA coding for a polypeptide having interferon activity, insertable in a vector, such as plasmid pBR322, and also to human interferon .beta.1 in highly purified form, and human interferon .beta.2 in highly purified form.

Abstract translation: 本发明涉及一种分离含有人成纤维细胞中编码干扰素的核苷酸序列的遗传物质（DNA）的方法，其包括在暴露于干扰素诱导剂时培养产生干扰素的细胞，将其暴露于所述诱导物，从所述诱导物中提取信使RNA 诱导细胞，纯化干扰素信使RNA，将信使RNA转录成DNA，并将DNA克隆在合适的载体中。 优选的细胞是人二倍体包皮细胞。 本发明还涉及一种用于工程化细菌菌株以产生干扰素多肽的方法，其包括将克隆的干扰素DNA引入合适的载体载体中。 优选的载体载体是大肠杆菌。 本发明还涉及高度纯化形式的人干扰素的mRNA，β1高度纯化形式的人干扰素的mRNA与β2高度纯化形式的人干扰素的mRNA与编码具有干扰素的多肽的DNA 活性，可插入载体，如质粒pBR322，以及高度纯化形式的人干扰素β1和高度纯化形式的人干扰素β2。

公开(公告)号：US5216128A

公开(公告)日：1993-06-01

申请号：US530580

申请日：1990-05-30

Applicant: Daniela Novick ,  Louise Chen ,  Hartmut Engelmann ,  Michel Revel ,  Menachem Rubinstein ,  David Wallach

Inventor： Daniela Novick ,  Louise Chen ,  Hartmut Engelmann ,  Michel Revel ,  Menachem Rubinstein ,  David Wallach

IPC: G01N33/53 ,  A61K38/00 ,  A61K38/21 ,  A61K39/395 ,  A61P35/00 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C07K14/00 ,  C07K14/52 ,  C07K14/54 ,  C07K14/555 ,  C07K14/565 ,  C07K14/705 ,  C07K14/715 ,  C07K16/00 ,  C12N15/12 ,  C12P21/00 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577

CPC classification number: C07K14/715 ,  A61K38/215 ,  C07K14/7155 ,  Y10S530/834

Abstract: Human natural IFN-.beta.2/IL-6 receptor and its soluble extracellular fragment are provided in substantially purified form and are shown to stimulate and to enhance beneficial effects of IFN-.beta.2/IL-6, such as its antiproliferative activity. Polyclonal and monoclonal antibodies raised against the soluble fragment of the receptor are also provided.

公开(公告)号：US5071963A

公开(公告)日：1991-12-10

申请号：US36104

申请日：1987-04-08

Applicant: Michel Revel ,  Judith Chebath

Inventor： Michel Revel ,  Judith Chebath

IPC: G01N33/53 ,  A61K38/43 ,  A61K39/395 ,  C07H21/04 ,  C07K7/08 ,  C07K14/00 ,  C07K14/435 ,  C07K14/555 ,  C07K16/00 ,  C07K16/40 ,  C07K19/00 ,  C12N1/20 ,  C12N1/21 ,  C12N9/00 ,  C12N9/12 ,  C12N9/16 ,  C12N15/00 ,  C12N15/09 ,  C12P21/00 ,  C12P21/02 ,  C12Q1/02 ,  C12Q1/25 ,  C12Q1/68 ,  C12R1/19 ,  C12R1/91 ,  G01N33/573

CPC classification number: C12N9/93 ,  C12Q1/6897 ,  G01N33/573 ,  Y10S435/81 ,  Y10S436/80 ,  Y10S436/804 ,  Y10S436/813 ,  Y10S530/806

Abstract: Human DNA encoding enzymes having (2'-5') oligo A synthetase has been sequenced. The amino acid sequences of the enzymes have been deduced. Antigenic peptides have been prepared and have been used to raise antibodies which recognize and immunoprecipitate the 40 kd, 46 kd, 67 kd and 100 kd forms of (2'-5') oligo A synthetase. Methods of monitoring interferon activity in a subject are presented.

Abstract translation: 编码具有（2'-5'）oligo A合成酶的人类DNA已被测序。 推测了酶的氨基酸序列。 已经制备抗原肽并且已经用于产生识别和免疫沉淀40kd，46kd，67kd和100kd形式的（2'-5'）oligo A合成酶的抗体。 介绍了受试者中干扰素活性的监测方法。

公开(公告)号：US08809052B2

公开(公告)日：2014-08-19

申请号：US12310495

申请日：2007-08-15

Applicant: Michel Revel ,  Judith Chebath ,  Michal Izrael ,  Rosalia Kaufman

Inventor： Michel Revel ,  Judith Chebath ,  Michal Izrael ,  Rosalia Kaufman

IPC: C12N5/071 ,  C12N5/00

CPC classification number: C12N5/0622 ,  A61K35/30 ,  C12N5/0619 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2506/02 ,  C12N2533/52 ,  C12N2533/90 ,  G01N33/5058

Abstract: A method of generating neural and glial cells is provided. The method comprising growing human stem cells under conditions which induce differentiation of said human stem cells into the neural and glial cells, said conditions comprising the presence of retinoic acid and an agent capable of down-regulating Bone Morphogenic Protein activity.

Abstract translation: 提供了一种产生神经和神经胶质细胞的方法。 该方法包括在诱导所述人类干细胞分化为神经和神经胶质细胞的条件下培养人类干细胞，所述条件包括视黄酸和能够下调骨形态发生蛋白活性的试剂的存在。

公开(公告)号：US07524928B2

公开(公告)日：2009-04-28

申请号：US11350094

申请日：2006-02-09

Applicant: Michel Revel ,  Carolina Abramovitch ,  Judith E. Chebath

Inventor： Michel Revel ,  Carolina Abramovitch ,  Judith E. Chebath

IPC: C07K14/00

CPC classification number: C12N9/1007 ,  A61K38/21 ,  C07K14/4702 ,  C07K14/555 ,  A61K2300/00

Abstract: Novel proteins IR1B1 and IR1B4 have been isolated which bind to the type I IFN receptor IFNAR1 and function in the cellular response to IFNs. DNA encoding such proteins in either the sense or anti-sense orientation can be administered to either enhance or inhibit the cellular response to IFNs. Antibodies to the proteins can be used for isolation of the new protein or for immunodetection thereof.

Abstract translation: 已经分离出新的蛋白质IR1B1和IR1B4，其结合I型IFN受体IFNAR1并在细胞对IFN的反应中起作用。 可以施用编码有义或反义方向的蛋白质的DNA，以增强或抑制对IFN的细胞应答。 蛋白质的抗体可用于分离新蛋白质或用于免疫检测。

公开(公告)号：US07264945B2

公开(公告)日：2007-09-04

申请号：US10309280

申请日：2002-12-04

Applicant: Michel Revel ,  Carolina Abramovitch ,  Judith E. Chebath

Inventor： Michel Revel ,  Carolina Abramovitch ,  Judith E. Chebath

IPC: C12N15/00 ,  C12N5/00

CPC classification number: C12N9/1007 ,  A61K38/21 ,  C07K14/4702 ,  A61K2300/00

Abstract: Novel proteins IR1B1 and IR1B4 have been isolated which bind to the type I IFN receptor IFNAR1 and function in the cellular response to IFNs. DNA encoding such proteins in either the sense or anti-sense orientation can be administered to either enhance or inhibit the cellular response to IFNs. Antibodies to the proteins can be used for isolation of the new protein or for immunodetection thereof.

Abstract translation: 已经分离出新的蛋白质IR1B1和IR1B4，其结合I型IFN受体IFNAR1并在细胞对IFN的反应中起作用。 可以施用编码有义或反义方向的蛋白质的DNA，以增强或抑制对IFN的细胞应答。 蛋白质的抗体可用于分离新蛋白质或用于免疫检测。

公开(公告)号：US20050220761A1

公开(公告)日：2005-10-06

申请号：US10498899

申请日：2002-12-31

Applicant: Shalom Haggiag ,  Michel Revel ,  Judith Chebath ,  Alon Levy ,  Peilin Zhang

Inventor： Shalom Haggiag ,  Michel Revel ,  Judith Chebath ,  Alon Levy ,  Peilin Zhang

IPC: A61K48/00 ,  A61K35/12 ,  A61K35/48 ,  A61K35/76 ,  A61K38/00 ,  A61K38/17 ,  A61K38/20 ,  A61K38/22 ,  A61K38/27 ,  A61P25/00 ,  A61P25/14 ,  A61P25/16 ,  A61P25/28 ,  A61K38/18 ,  A61K38/19

CPC classification number: A61K35/30 ,  A61K38/1793 ,  A61K38/204 ,  A61K2300/00

Abstract: The present invention provides for the use of IL6R/IL6 chimera for the manufacture of medicaments for the treatment of neurological diseases and disorders.

Abstract translation: 本发明提供IL6R / IL6嵌合体用于制备用于治疗神经疾病和病症的药物的用途。