公开(公告)号：US07220445B2

公开(公告)日：2007-05-22

申请号：US10492782

申请日：2002-10-25

Applicant: Benjamin Bower ,  Frits Goedegebuur ,  Kathleen A. Clarkson ,  Scott D. Power

Inventor： Benjamin Bower ,  Frits Goedegebuur ,  Kathleen A. Clarkson ,  Scott D. Power

IPC: A23J3/20 ,  A23J3/34 ,  C12N9/16 ,  C07H21/04

CPC classification number: C12N9/16

Abstract: DNA is provided which encodes an enzyme having phytase activity isolated from Penicillium, Fusarium, Humicola and Emericella. Also provided for is a method of isolating DNA encoding an enzyme having phytase activity from organisms which possess such DNA, transformation of the DNA into a suitable host organism, expression of the transformed DNA and the use of the expressed phytase protein in feed as a supplement.

Abstract translation: 提供了编码具有从青霉，镰刀霉，腐质霉和Emericicella分离的植酸酶活性的酶的DNA。 还提供了分离编码具有植酸酶活性的酶的DNA从具有这种DNA的生物中分离的DNA的方法，将DNA转化为合适的宿主生物体，转化的DNA的表达和在饲料中使用表达的植酸酶蛋白作为补充 。

公开(公告)号：US5849549A

公开(公告)日：1998-12-15

申请号：US468698

申请日：1995-06-06

Applicant: Christopher C. Barnett ,  Leif P. Solheim ,  Colin Mitchinson ,  Scott D. Power ,  Carol A. Requadt

Inventor： Christopher C. Barnett ,  Leif P. Solheim ,  Colin Mitchinson ,  Scott D. Power ,  Carol A. Requadt

IPC: C12N15/09 ,  C11D3/386 ,  C12N1/21 ,  C12N5/10 ,  C12N9/26 ,  C12N9/28 ,  C12N15/56 ,  C12N15/75 ,  C12P19/14

CPC classification number: C12N9/2417 ,  C11D3/386 ,  C12N15/75 ,  C12P19/14

Abstract: Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.

Abstract translation: 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。

公开(公告)号：US5429950A

公开(公告)日：1995-07-04

申请号：US860468

申请日：1992-03-30

Applicant: Scott D. Power ,  Johanna M. A. van Kimmenade ,  Louann Carlomagno

Inventor： Scott D. Power ,  Johanna M. A. van Kimmenade ,  Louann Carlomagno

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/20 ,  C12N9/56 ,  C12N15/75 ,  C12R1/125 ,  C12N1/20 ,  C12N15/00

CPC classification number: C12N9/54 ,  C12N15/75 ,  C12N9/20 ,  C07K2319/00 ,  C07K2319/02

Abstract: There are described processes for expressing a lipase in a heterologous host. Further described are transformed cells comprising heterologous DNA and particular plasmids containing heterologous fusion DNA constructs.

Abstract translation: 描述了在异源宿主中表达脂肪酶的方法。 进一步描述包含异源DNA和含有异源融合DNA构建体的特定质粒的转化细胞。

公开(公告)号：US5182204A

公开(公告)日：1993-01-26

申请号：US807786

申请日：1991-12-11

Applicant: David A. Estell ,  Richard R. Bott ,  Scott D. Power ,  James A. Wells

Inventor： David A. Estell ,  Richard R. Bott ,  Scott D. Power ,  James A. Wells

IPC: C11D3/386 ,  C12N9/54 ,  C12N9/56 ,  C12N15/10 ,  C12N15/55 ,  C12N15/75 ,  C12N1/21

CPC classification number: C12R1/07 ,  C11D3/386 ,  C12N15/102 ,  C12N15/75 ,  C12N9/54 ,  Y10S435/832

Abstract: Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to encode the substitution of an amino acid in the amino acid sequence of a precursor carbonyl hydrolase. Such mutants have properties which are different than the precursor hydrolase.

Abstract translation: 衍生自天然或重组非人羰基水解酶的DNA序列的新型羰基水解酶突变体。 突变体羰基水解酶通常通过体外修饰编码天然存在或重组的羰基水解酶的前体DNA序列获得，以编码前体羰基水解酶的氨基酸序列中的氨基酸的取代。 这些突变体具有与前体水解酶不同的性质。

公开(公告)号：US08206966B2

公开(公告)日：2012-06-26

申请号：US12263886

申请日：2008-11-03

Applicant: Luis G. Cascāo-Pereira ,  James T. Kellis, Jr. ,  Bradley A. Paulson ,  Scott D. Power ,  Sandra W. Ramer ,  Vivek Sharma ,  Andrew Shaw ,  Jayarama K. Shetty ,  Donald E. Ward

Inventor： Luis G. Cascāo-Pereira ,  James T. Kellis, Jr. ,  Bradley A. Paulson ,  Scott D. Power ,  Sandra W. Ramer ,  Vivek Sharma ,  Andrew Shaw ,  Jayarama K. Shetty ,  Donald E. Ward

IPC: C12N9/26 ,  C12N9/28 ,  C12N9/14 ,  C12N9/16 ,  C12N1/20 ,  C12N15/00 ,  C12P19/14 ,  C12P21/06 ,  C12P19/34 ,  C07H21/04 ,  C12C11/00 ,  A21D2/16 ,  C07K1/00

CPC classification number: C11D3/38681 ,  C11D3/386 ,  C12N9/2417 ,  D06M16/003

Abstract: Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.

Abstract translation: 公开了包含α-淀粉酶变体的组合物，其具有α-淀粉酶活性，并且相对于其来源的亲本AmyS样α-淀粉酶表现出改变的性质。 组合物包含另外的酶如植酸酶。 还公开了使用组合物的方法和与其相关的试剂盒。

公开(公告)号：US20110256608A1

公开(公告)日：2011-10-20

申请号：US13082979

申请日：2011-04-08

Applicant: Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

Inventor： Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

IPC: C12N9/42 ,  C12N1/21 ,  C12N9/00 ,  C12N15/63

CPC classification number: C07K14/811 ,  A61K8/64 ,  A61Q7/02

Abstract: The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care.

Abstract translation: 本发明涉及包含结合靶蛋白的肽的修饰变体Bowman Birk蛋白酶抑制剂蛋白（BBPI），并且被进一步修饰以具有比未修饰的BBPI更大的蛋白酶抑制活性和/或以更高的产量产生。 本发明包括多核苷酸构建体和含有编码修饰变体BBPI的多核苷酸序列的表达载体，表达和产生修饰变体BBPI的转化宿主细胞，修饰变体BBPI蛋白，包含修饰变体BBPI的组合物和制备方法 并在个人护理中使用修改后的变体BBPI。

公开(公告)号：US07947475B2

公开(公告)日：2011-05-24

申请号：US12779793

申请日：2010-05-13

Applicant: Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

Inventor： Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

IPC: C12P21/00

CPC classification number: C07K14/811 ,  A61K8/64 ,  A61Q7/02

Abstract: The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care.

Abstract translation: 本发明涉及包含结合靶蛋白的肽的修饰变体Bowman Birk蛋白酶抑制剂蛋白（BBPI），并且被进一步修饰以具有比未修饰的BBPI更大的蛋白酶抑制活性和/或以更高的产量产生。 本发明包括多核苷酸构建体和含有编码修饰变体BBPI的多核苷酸序列的表达载体，表达和产生修饰变体BBPI的转化宿主细胞，修饰变体BBPI蛋白，包含修饰变体BBPI的组合物和制备方法 并在个人护理中使用修改后的变体BBPI。

公开(公告)号：US20110097778A1

公开(公告)日：2011-04-28

申请号：US12990201

申请日：2009-04-23

Applicant: Scott D. Power ,  Andrew Shaw

Inventor： Scott D. Power ,  Andrew Shaw

IPC: C12N9/26 ,  C07H21/00 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10 ,  C12S9/00 ,  C12S11/00 ,  C12P7/02 ,  C12P7/06 ,  C11D3/386 ,  A23L1/05

CPC classification number: C12N9/96 ,  A23L29/06 ,  C11D3/38618 ,  C12N9/2417 ,  C12P19/14 ,  Y02E50/17

Abstract: Chimeric alpha amylases having the characteristics of high thermostability and good performance in starch degradation, especially high-temperature liquefaction processes, are provided. The alpha-amylases are chimeras of AmyL and AmyS enzymes, and are useful in starch degradation processes. Methods of making the chimeric enzymes, and methods of using the chimeric alpha-amylases for liquefaction, cleaning starch residue from a surface, and treating woven material to remove coatings. Kits for practicing the methods are provided. Polynucleotides encoding the chimeric amylases, vectors, and expression hosts also are provided.

Abstract translation: 提供了具有高淀粉降解特性，特别是高温液化工艺的高热稳定性和良好性能的嵌合α淀粉酶。 α-淀粉酶是AmyL和AmyS酶的嵌合体，并且可用于淀粉降解过程。 制备嵌合酶的方法，以及使用嵌合α-淀粉酶液化的方法，从表面清洗淀粉残渣，以及处理织造材料以除去涂层。 提供实践方法的工具包。 还提供了编码嵌合淀粉酶，载体和表达宿主的多核苷酸。

公开(公告)号：US07772181B2

公开(公告)日：2010-08-10

申请号：US12252032

申请日：2008-10-15

Applicant: Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

Inventor： Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

IPC: A61K38/00 ,  A61K8/00

CPC classification number: A61K8/64 ,  A61K38/55 ,  A61Q1/02 ,  A61Q1/04 ,  A61Q1/06 ,  A61Q1/10 ,  A61Q3/00 ,  A61Q3/02 ,  A61Q5/006 ,  A61Q5/02 ,  A61Q5/06 ,  A61Q5/12 ,  A61Q17/04 ,  A61Q19/00 ,  A61Q19/002 ,  A61Q19/04 ,  A61Q19/10

Abstract: The present invention relates to compositions that comprise modified variant Bowman Birk Protease Inhibitor proteins (BBPIs). The modified variant BBPIs comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses the compositions comprising the modified variant BBPIs, and the methods for making and using the compositions in personal care.

Abstract translation: 本发明涉及包含修饰的变体Bowman Birk蛋白酶抑制蛋白（BBPI）的组合物。 修饰的变体BBPI包含结合靶蛋白的肽，并且被进一步修饰以具有比未修饰的BBPI更大的蛋白酶抑制活性和/或以更高的产量产生。 本发明包括包含修饰变体BBPI的组合物，以及在个人护理中制备和使用组合物的方法。

公开(公告)号：US07696173B2

公开(公告)日：2010-04-13

申请号：US12172921

申请日：2008-07-14

Applicant: Katherine Collier ,  Grant Ganshaw ,  Hans De Nobel ,  Scott D. Power ,  Anita Van Kimmenade ,  Marc Kolkman ,  Jeffrey Miller ,  Brian Schmidt ,  Gudrun Vogtentanz ,  David Estell

Inventor： Katherine Collier ,  Grant Ganshaw ,  Hans De Nobel ,  Scott D. Power ,  Anita Van Kimmenade ,  Marc Kolkman ,  Jeffrey Miller ,  Brian Schmidt ,  Gudrun Vogtentanz ,  David Estell

IPC: A61K38/16

CPC classification number: C07K14/81 ,  A61K38/00 ,  C07K7/06 ,  C07K14/811 ,  C07K2319/00 ,  C12N15/62

Abstract: The present invention provides compositions and methods related to expression of protease inhibitors and variants thereof in bacterial species. The present invention further provides fusion nucleic acids, vectors, fusion polypeptides, and processes for obtaining the protease inhibitors.

Abstract translation: 本发明提供了与细菌种中的蛋白酶抑制剂及其变体的表达相关的组合物和方法。 本发明进一步提供融合核酸，载体，融合多肽以及获得蛋白酶抑制剂的方法。