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22 条记录 (耗时 0.014 秒)

    Vinyl chloride polymerization with recycle return projecting into gas phase region of reactor
    13.
    发明授权
    Vinyl chloride polymerization with recycle return projecting into gas phase region of reactor 失效
    乙烯基氯化物聚合反应再循环回到反应器的气相区域

    公开(公告)号：US5447999A

    公开(公告)日：1995-09-05

    申请号：US193866

    申请日：1994-07-09

    申请人： Takuya Ueda ,  Yoshihiro Shirota ,  Yoshihiko Hirai ,  Toshiaki Maruyama ,  Tadashi Amano

    发明人： Takuya Ueda ,  Yoshihiro Shirota ,  Yoshihiko Hirai ,  Toshiaki Maruyama ,  Tadashi Amano

    IPC分类号： B01J19/00 ,  C08F2/00 ,  C08F2/16 ,  C08F14/06 ,  C08F114/06

    CPC分类号： C08F2/002 ,  B01J19/002 ,  C08F14/06 ,  B01J2219/00252

    摘要： A polymerization process, comprises a polymerization vessel, a reflux condenser installed outside the polymerization vessel and a pipe connected between the reflux condenser and a wall of the polymerization vessel with an end thereof opening into a gaseous phase region inside the polymerization vessel, for returning the liquid condensate to the polymerization vessel, wherein said end projects from an inner surface of the wall of the polymerization vessel into the gaseous phase region. In polymerizing a monomer having an ethylenically unsaturated double bond using the polymerization apparatus, the quantity of heat removed by the reflux condenser is increased stepwise or continuously with progress of the polymerization, such that not less than 20% of the total reaction heat generated during the whole course of the polymerization is removed by the reflux condenser. With 100 or more repeated polymerization runs, polymer scale deposition inside the vessel is minimal even below the pipe end.

    摘要翻译： 聚合方法包括聚合容器，安装在聚合容器外部的回流冷凝器和连接在回流冷凝器和聚合容器壁之间的管道，其端部开口到聚合容器内的气相区域， 液体冷凝物到聚合容器中，其中所述端部从聚合容器的壁的内表面突出到气相区域中。 在使用聚合装置聚合具有烯键式不饱和双键的单体时，由回流冷凝器除去的热量随着聚合的进行而逐步或连续地增加，使得不少于20℃时产生的反应热总量 通过回流冷凝器除去整个聚合过程。 通过100次或更多次的反复聚合运行，在管端下方，容器内的聚合物垢沉积是最小的。

    HIGH-SPEED MATURATION METHOD FOR AN OLIGONUCLEOTIDE LIBRARY FOR THE PURPOSE OF PREPARING A PROTEIN LIBRARY
    15.
    发明申请
    HIGH-SPEED MATURATION METHOD FOR AN OLIGONUCLEOTIDE LIBRARY FOR THE PURPOSE OF PREPARING A PROTEIN LIBRARY 有权
    用于制备蛋白质库的目的的寡核苷酸文库的高速成熟方法

    公开(公告)号：US20130143773A1

    公开(公告)日：2013-06-06

    申请号：US13806470

    申请日：2011-06-23

    申请人： Takuya Ueda ,  Takashi Kanamori ,  Kanehisa Kojoh ,  Shizue Katoh ,  Akira Miyakoshi

    发明人： Takuya Ueda ,  Takashi Kanamori ,  Kanehisa Kojoh ,  Shizue Katoh ,  Akira Miyakoshi

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1065 ,  C12N15/1041 ,  C12N15/1093 ,  C12N15/67

    摘要： The present invention provides a method of producing a maturated oligonucleotide library, including a step of obtaining a terminal-modified product of a maturation target oligonucleotide library, including adding a tag sequence to the 5′ terminus of the maturation target oligonucleotide library and an arrest sequence, which stalls translation elongation on a ribosome, to the 3′ terminus of the maturation target oligonucleotide library, a step of transcribing the terminal-modified sequence product to give a transcript, and a step of in vitro translation for translating the transcript in vitro, wherein the maturation target oligonucleotide library is a random oligonucleotide library.

    摘要翻译： 本发明提供一种生产成熟寡核苷酸文库的方法，包括获得成熟靶寡核苷酸文库的末端修饰产物的步骤，包括向成熟靶寡核苷酸文库的5'末端加入标签序列， ，其将核糖体上的翻译延伸失活到成熟靶寡核苷酸文库的3'末端，转录末端修饰的序列产物以产生转录物的步骤，以及用于体外翻译转录物的体外翻译步骤， 其中成熟靶寡核苷酸文库是随机寡核苷酸文库。

    Process for producing peptides by using in vitro transcription/translation system
    16.
    发明授权
    Process for producing peptides by using in vitro transcription/translation system 有权
    通过使用体外转录/翻译系统生产肽的方法

    公开(公告)号：US07118883B2

    公开(公告)日：2006-10-10

    申请号：US09983067

    申请日：2001-10-23

    申请人： Akio Inoue ,  Yoshihiro Shimizu ,  Takuya Ueda

    发明人： Akio Inoue ,  Yoshihiro Shimizu ,  Takuya Ueda

    IPC分类号： C12P21/06

    CPC分类号： C12P21/02

    摘要： The present invention relates to a reaction system whereby a peptide produced in an in vitro peptide synthesis system can be efficiently isolated at a high purity from the reaction system. Thus the present invention is a process for producing a peptide or a peptide derivative by using a reaction system of transcribing a DNA into an RNA and then translating the RNA produced or a reaction system of translating an RNA in vitro wherein at least one protein component of the reaction system is labeled with a first substance which adheres to a second substance, and said second substance is used as an adsorbent for capturing said labeled protein components after translating.

    摘要翻译： 本发明涉及一种反应体系，其中可以从体系肽合成体系中产生的肽以高纯度从反应体系中高效分离。 因此，本发明是通过使用将DNA转录成RNA，然后翻译生成的RNA或体外翻译RNA的反应体系的反应体系来生产肽或肽衍生物的方法，其中至少一种蛋白质成分 反应体系用粘附于第二物质的第一物质标记，所述第二物质用作在翻译后捕获所述标记的蛋白质成分的吸附剂。

    POLYNUCLEOTIDE CONSTRUCT CAPABLE OF DISPLAYING FAB IN A CELL-FREE TRANSLATION SYSTEM, AND METHOD FOR MANUFACTURING AND SCREENING FAB USING SAME
    19.
    发明申请
    POLYNUCLEOTIDE CONSTRUCT CAPABLE OF DISPLAYING FAB IN A CELL-FREE TRANSLATION SYSTEM, AND METHOD FOR MANUFACTURING AND SCREENING FAB USING SAME 有权
    可在无细胞翻译系统中显示FAB的多核苷酸结构，以及使用其制造和筛选FAB的方法

    公开(公告)号：US20130288908A1

    公开(公告)日：2013-10-31

    申请号：US13990654

    申请日：2011-11-30

    申请人： Yasuhiro Fujino ,  Risako Fujita ,  Kouichi Wada ,  Kotomi Oda ,  Takuya Ueda ,  Yoshihiro Shimizu ,  Takashi Kanamori

    发明人： Yasuhiro Fujino ,  Risako Fujita ,  Kouichi Wada ,  Kotomi Oda ,  Takuya Ueda ,  Yoshihiro Shimizu ,  Takashi Kanamori

    IPC分类号： G01N33/68

    CPC分类号： G01N33/6854 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/55 ,  C12N15/1041 ,  C12N15/1062 ,  C12N15/1075 ,  G01N33/6857

    摘要： The polynucleotide construct of (1) or (2) below is used to perform ribosome display, CIS display and/or mRNA display in order to screen a Fab against an antigen of interest: (1) a polynucleotide construct which monocistronically comprises a ribosome-binding sequence, Fab first chain-coding sequence, linker peptide-coding sequence, Fab second chain-coding sequence and scaffold-coding sequence in this order, and further comprises at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself, and (2) a polynucleotide construct which comprises a Fab first chain-expressing cistron and a Fab second chain-expressing cistron each containing a ribosome-binding sequence, a Fab first chain-coding sequence or Fab second chain-coding sequence, and a scaffold-coding sequence in this order, the first Fab-expressing cistron further comprising at its 3′-end a ribosome stall sequence, said Fab second chain-expressing cistron further comprising at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself.

    摘要翻译： 以下的（1）或（2）的多核苷酸构建体用于进行核糖体展示，CIS显示和/或mRNA显示，以便针对感兴趣的抗原筛选Fab：（1）单核细胞系包含核糖体 - 结合序列，Fab第一链编码序列，接头肽编码序列，Fab第二链编码序列和支架编码序列，并且在其3'末端还包含与Fab保持复合物所必需的结构 其自身编码，和（2）包含Fab第一链表达顺反子和Fab第二链表达顺反子的多核苷酸构建体，其各自含有核糖体结合序列，Fab第一链编码序列或Fab第二链编码序列 和支架编码序列，所述第一Fab表达顺反子在其3'末端进一步包含核糖体失速序列，所述Fab第二链表达顺反子在其3'末端还包含结构ne 维持与Fab自身编码的复合物的塞车。