公开(公告)号：US5972710A

公开(公告)日：1999-10-26

申请号：US829679

申请日：1997-03-31

申请人： Bernhard H. Weigl ,  Paul Yager ,  James P. Brody ,  Mark R. Holl ,  Margaret Kenny ,  David Schutte ,  Gregory Hixson ,  M. Diane Zebert ,  Andrew Kamholz ,  Caicai Wu ,  Eric Altendorf

发明人： Bernhard H. Weigl ,  Paul Yager ,  James P. Brody ,  Mark R. Holl ,  Margaret Kenny ,  David Schutte ,  Gregory Hixson ,  M. Diane Zebert ,  Andrew Kamholz ,  Caicai Wu ,  Eric Altendorf

IPC分类号： G01N37/00 ,  B01F5/04 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  G01N15/06 ,  G01N15/14 ,  G01N30/00 ,  G01N30/38 ,  G01N30/60 ,  G01N30/74 ,  G01N31/20 ,  G01N35/08 ,  G01N21/64

CPC分类号： B01F5/0403 ,  B01F13/0059 ,  B01F13/0093 ,  B01J19/0093 ,  B01L3/502776 ,  G01N30/0005 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00952 ,  B01L2200/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  G01N15/14 ,  G01N30/38 ,  G01N30/6095 ,  G01N30/74 ,  G01N35/085 ,  Y10S366/01 ,  Y10T436/25375 ,  Y10T436/2575

摘要： A channel-cell system is provided for detecting the presence and/or measuring the presence of analyte particles in a sample stream comprising: a) a laminar flow channel; b) two inlets in fluid connection with the laminar flow channel for respectively conducting into the laminar flow channel (1) an indicator stream which may comprise an indicator substance which indicates the presence of the analyte particles by a detectable change in property when contacted with the analyte particles, and (2) the sample stream; c) wherein the laminar flow channel has a depth sufficiently small to allow laminar flow of the streams and a length sufficient to allow particles of the analyte to diffuse into the indicator stream to the substantial exclusion of the larger particles in the sample stream to form a detection area; and d) an outlet for conducting the streams out of the laminar flow channel to form a single mixed stream.

摘要翻译： 提供了一种通道细胞系统，用于检测样品流中存在和/或测量分析物颗粒的存在，其包括：a）层流通道; b）与层流通道流体连接的两个入口，用于分别导入层流通道（1）中，指示物流可包含指示物质，其指示物质与当与 分析物颗粒，和（2）样品流; c）其中层流通道具有足够小的深度以允许流的层流，并且足够的长度允许分析物的颗粒扩散到指示物流中，从而基本排除样品流中的较大颗粒，形成 检测区; 和d）用于将物流从层流通道导出以形成单一混合流的出口。

公开(公告)号：US07141429B2

公开(公告)日：2006-11-28

申请号：US10268620

申请日：2002-10-09

申请人： Matthew S. Munson ,  Catherine R. Cabrera ,  Paul Yager ,  Anson Hatch ,  Andrew Kamholz

发明人： Matthew S. Munson ,  Catherine R. Cabrera ,  Paul Yager ,  Anson Hatch ,  Andrew Kamholz

IPC分类号： G01N35/08 ,  B01D17/00

CPC分类号： B01F13/0076 ,  B01F13/0093 ,  B01F2005/0031 ,  B01F2215/0431 ,  B01F2215/0459 ,  B01J2219/00912 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0647 ,  G01N27/44704 ,  G01N27/44726 ,  Y10T436/117497 ,  Y10T436/118339

摘要： This invention provides methods for using liquid junction potentials to control the transport of charged particles in fluid streams that are in laminar flow within microfluidic channels. Applications of the methods of this invention include sample preconditioning (removal of interfering substances), electrophoretic separation (fractionation) of charged particles, enhanced or delayed mixing of charged particles across a fluid interface relative to diffusion only, focusing charged particles in a fluid stream in one or two dimensions, and concentration of charged reactants at a fluid interface.

公开(公告)号：US20060196771A1

公开(公告)日：2006-09-07

申请号：US11419717

申请日：2006-05-22

申请人： Matthew Munson ,  Catherine Cabrera ,  Paul Yager ,  Anson Hatch ,  Andrew Kamholz

发明人： Matthew Munson ,  Catherine Cabrera ,  Paul Yager ,  Anson Hatch ,  Andrew Kamholz

IPC分类号： C07K1/26 ,  G01N27/447

CPC分类号： B01F13/0076 ,  B01F13/0093 ,  B01F2005/0031 ,  B01F2215/0431 ,  B01F2215/0459 ,  B01J2219/00912 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0647 ,  G01N27/44704 ,  G01N27/44726 ,  Y10T436/117497 ,  Y10T436/118339

摘要： This invention provides methods for using liquid junction potentials to control the transport of charged particles in fluid streams that are in laminar flow within microfluidic channels. Applications of the methods of this invention include sample preconditioning (removal of interfering substances), electrophoretic separation (fractionation) of charged particles, enhanced or delayed mixing of charged particles across a fluid interface relative to diffusion only, focusing charged particles in a fluid stream in one or two dimensions, and concentration of charged reactants at a fluid interface.

摘要翻译： 本发明提供了使用液体接合电位来控制带电粒子在微流体通道内处于层流的流体流中的运输的方法。 本发明方法的应用包括样品预处理（去除干扰物质），带电粒子的电泳分离（分级），相对于仅扩散的带电粒子在流体界面上的增强或延迟混合，将带电粒子聚集在流体流中 一个或两个维度，以及带电反应物在流体界面处的浓度。

公开(公告)号：US06541213B1

公开(公告)日：2003-04-01

申请号：US09574797

申请日：2000-05-19

申请人： Bernhard H. Weigl ,  Paul Yager ,  Andrew Kamholz ,  Anson Hatch

发明人： Bernhard H. Weigl ,  Paul Yager ,  Andrew Kamholz ,  Anson Hatch

IPC分类号： G01N33533

CPC分类号： B01F5/0403 ,  B01F13/0059 ,  B01F13/0093 ,  B01J19/0093 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0487 ,  G01N15/10 ,  G01N15/14 ,  G01N30/0005 ,  G01N30/38 ,  G01N30/6095 ,  G01N30/74 ,  G01N33/558 ,  G01N35/085 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Methods and apparatuses are provided for determining presence and concentration of analytes by exploiting molecular binding reactions and differential diffusion rates. Analyte particles and binding particles are allowed to diffuse toward each other, and slowing of the diffusion front is detected when they meet. From the position of the diffusion front, presence and concentration of analyte particles can be determined. One embodiment provides a competitive immunoassay in a microfluidic format. This diffusion immunoassay (DIA) relies on measuring the concentration of labeled antigen along one dimension of a microchannel after allowing it to diffuse for a short time into a region containing specific antibodies. A simple microfluidic device, the T-Sensor, was used to implement a DIA to measure the concentration of phenytoin, a small drug molecule. Concentrations of analyte over the range of 50 to 1600 nM can be measured in less than a minute. The assay is homogeneous, rapid, requires only microliter volumes of reagents and sample, and is applicable to a wide range of analytes, including therapeutic drugs, molecular biological markers, and environmental contaminants. Methods for separating particles of similar size in a diffusion separator are also provided.

公开(公告)号：US06482306B1

公开(公告)日：2002-11-19

申请号：US09404454

申请日：1999-09-22

申请人： Paul Yager ,  Mark R. Holl ,  Andrew Kamholz ,  Catherine Cabrera ,  Katerina Macounova

发明人： Paul Yager ,  Mark R. Holl ,  Andrew Kamholz ,  Catherine Cabrera ,  Katerina Macounova

IPC分类号： B01F1300

CPC分类号： B01F13/0076 ,  B01F13/0001 ,  B01F13/0005 ,  B01F13/0077 ,  B01L3/5027 ,  Y10T137/2087 ,  Y10T137/2191

摘要： An electroösmotic mixing device and a method for mixing one or more fluids for use in meso- or microfluidic device applications. The mixing device provides batch or continuous mixing of one or more fluids in meso- or microfluidic channels. An electric field is generated in the channel in substantial contact with chargeable surfaces therein. No alterations of the geometry of existing flow paths need be made, and the degree of mixing in the device can be controlled by the length of the electrodes, the flow rate past the electrodes, and the voltage applied to those electrodes. The degree of mixing is affected by choice of materials for the chargeable surface (in some cases by the selection of materials or coatings for channel walls) and the ionic strength of the fluids and the type and concentration of ions in the fluids. The ionic strength of fluids to be mixed is sufficiently low to allow electroosmotic flow. The method and device of this invention is preferably applied to fluids to having low ionic strength less than or equal to about 1 mM.

摘要翻译： 一种电渗混合装置和用于混合用于中等或微流体装置应用的一种或多种流体的方法。 混合装置在中等或微流体通道中提供一种或多种流体的间歇或连续混合。 在通道中产生与其中的可充电表面实质接触的电场。 不需要改变现有流路的几何形状，并且可以通过电极的长度，通过电极的流速和施加到这些电极的电压来控制装置中的混合程度。 混合的程度受到可充电表面的材料选择（在某些情况下通过选择通道壁的材料或涂层）以及流体的离子强度以及流体中离子的类型和浓度的影响。 要混合的流体的离子强度足够低以允许电渗流。 本发明的方法和装置优选应用于具有小于或等于约1mM的低离子强度的流体。

公开(公告)号：US06454945B1

公开(公告)日：2002-09-24

申请号：US09703764

申请日：2000-11-01

申请人： Bernhard H. Weigl ,  Paul Yager ,  James P. Brody ,  Mark R. Holl ,  Fred K. Forster ,  Eric Altendorf ,  Paul C. Galambos ,  Margaret Kenny ,  David Schutte ,  Gregory Hixson ,  Diane Zebert ,  Andrew Kamholz ,  Caicai Wu

发明人： Bernhard H. Weigl ,  Paul Yager ,  James P. Brody ,  Mark R. Holl ,  Fred K. Forster ,  Eric Altendorf ,  Paul C. Galambos ,  Margaret Kenny ,  David Schutte ,  Gregory Hixson ,  Diane Zebert ,  Andrew Kamholz ,  Caicai Wu

IPC分类号： B02D1100

CPC分类号： B01D11/0492 ,  A61M1/14 ,  B01D11/0496 ,  B01L3/5027 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0487 ,  G01N15/1484 ,  Y10T436/25375

摘要： This invention provides microfabricated systems for extraction of desired particles from a sample stream containing desired and undesired particles. The sample stream is placed in laminar flow contact with an extraction stream under conditions in which inertial effects are negligible. The contact between the two streams is maintained for a sufficient period of time to allow differential transport of the desired particles from the sample stream into the extraction stream. In a preferred embodiment the differential transport mechanism is diffusion. The extraction system of this invention coupled to a microfabricated diffusion-based mixing device and/or sensing means allows picoliter quantities of fluid to be processed or analyzed on devices no larger than silicon wafers. Such diffusion-based mixing or sensing devices are preferably channel cell systems for detecting the presence and/or measuring the quantity of analyte particles in a sample stream.

摘要翻译： 本发明提供了用于从含有所需和不需要的颗粒的样品流中提取所需颗粒的微制造系统。 在惯性效应可忽略的条件下，将样品流与提取流层流接触。 保持两个物流之间的接触足够的时间，以便将期望的颗粒从样品流中差分输送到萃取物流中。 在优选实施例中，差分传送机构是扩散。 本发明的提取系统耦合到基于微制造的基于扩散的混合装置和/或感测装置允许在不大于硅晶片的装置上处理或分析皮升数量的流体。 这种基于扩散的混合或感测装置优选是用于检测样品流中分析物颗粒的存在和/或测量量的通道电池系统。