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    Design and synthesis of bispecific reagents: use of double stranded DNAs as chemically and spatially defined cross-linkers
    12.
    发明授权
    Design and synthesis of bispecific reagents: use of double stranded DNAs as chemically and spatially defined cross-linkers 失效
    双特异性试剂的设计和合成：使用双链DNA作为化学和空间定义的交联剂

    公开(公告)号：US5849878A

    公开(公告)日：1998-12-15

    申请号：US481065

    申请日：1995-06-07

    Applicant: Charles R. Cantor ,  Roy S. Chuck ,  Doris B. Tse

    Inventor： Charles R. Cantor ,  Roy S. Chuck ,  Doris B. Tse

    IPC: C07K16/28 ,  C07K16/46 ,  C07K17/00 ,  C07K19/00

    CPC classification number: C07K16/2812 ,  C07K16/2809 ,  C07K16/468

    Abstract: The invention relates to bis-protein-DNA conjugates. A protein having a specific ligand binding activity is covalently linked to each end of a derivatized DNA molecule. These bis-protein-DNA conjugates can be used for immunoassays, PCR assays and measuring distances between proteins at up to 3.4 A resolution. The invention also relates to methods of synthesizing these bis-protein-DNA conjugates. Synthesis of the conjugates entails derivatizing the 5' or 3' end of a DNA oligonucleotide and covalently linking that DNA to a protein. The DNA can be conjugated to the proteins, including antibodies or Fab' fragments, using disulfide bond linkage.

    Abstract translation: 本发明涉及双蛋白-DNA缀合物。 具有特异性配体结合活性的蛋白质与衍生的DNA分子的每个末端共价连接。 这些双蛋白-DNA缀合物可用于免疫测定，PCR测定和测量蛋白质之间的距离，高达3.4A分辨率。 本发明还涉及合成这些双蛋白-DNA缀合物的方法。 共轭物的合成需要衍生DNA寡核苷酸的5'或3'末端并共价连接该DNA与蛋白质。 使用二硫键可将DNA与蛋白质缀合，包括抗体或Fab'片段。

    Nucleic acid quantification products and processes
    15.
    发明授权
    Nucleic acid quantification products and processes 有权

    公开(公告)号：US10533215B2

    公开(公告)日：2020-01-14

    申请号：US13129797

    申请日：2009-11-20

    Applicant: Charles R. Cantor

    Inventor： Charles R. Cantor

    IPC: C12Q1/68 ,  C12Q1/6837

    Abstract: Described herein are products and processes for nucleic acid quantification, which are in part useful for detecting and determining the nucleotide sequence of rare nucleic acids (i.e., low copy number nucleic acids) in a sample. Such products and processes are useful for reducing the dynamic range among different nucleic acid species.

    Methods and compositions for the analysis of nucleic acids
    16.
    发明授权
    Methods and compositions for the analysis of nucleic acids 有权
    用于分析核酸的方法和组合物

    公开(公告)号：US08852864B2

    公开(公告)日：2014-10-07

    申请号：US12863169

    申请日：2009-01-16

    Applicant: Charles R. Cantor

    Inventor： Charles R. Cantor

    IPC: C12Q1/68

    CPC classification number: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2525/161 ,  C12Q2563/185

    Abstract: Provided herein are compositions and methods for analysis of nucleic acids, including, methods and compositions for genotyping, haplotyping, sequencing and performing other genetic and epigenetic analysis on nucleic acids, for example. In some embodiments, methods and compositions suitable for whole-genome sequencing on single molecules of nucleic acid are provided. In some embodiments, analysis of single molecules of nucleic acid are performed in conjunction with nanopores and/or nanopore devices.

    Abstract translation: 本文提供了用于核酸分析的组合物和方法，包括用于基因分型，单倍型，测序和对核酸进行其他遗传和表观遗传学分析的方法和组合物。 在一些实施方案中，提供适用于单分子核酸上的全基因组测序的方法和组合物。 在一些实施方案中，结合纳米孔和/或纳米孔装置进行单分子核酸的分析。

    Haplotype Analysis
    18.
    发明申请
    Haplotype Analysis 有权
    单倍型分析

    公开(公告)号：US20120295263A1

    公开(公告)日：2012-11-22

    申请号：US13565105

    申请日：2012-08-02

    Applicant: Charles R. Cantor ,  Chunming Ding

    Inventor： Charles R. Cantor ,  Chunming Ding

    IPC: G01N27/62

    CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2527/137 ,  C12Q2537/143 ,  C12Q2565/627

    Abstract: The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.

    Abstract translation: 本发明提供了高通量单倍型分析的有效方法。 可以通过多个平行的单分子稀释物中的单个核酸分子的多重PCR来同时并可靠地测定几种多态性核酸标记，例如SNP，并且随后对来自这些平行的单分子多重PCR反应的结果的统计分析导致可靠的测定 的单倍体存在于受试者。 核酸标记在染色体上可以彼此有任何距离。 此外，分析重叠的DNA标记物的方法可用于将较小的单倍型连接到较大的单元型中。 因此，本发明为诊断单倍型和鉴定新型单体型提供了强大的新工具。

    METHOD FOR NON-INVASIVE PRENATAL DIAGNOSIS
    19.
    发明申请
    METHOD FOR NON-INVASIVE PRENATAL DIAGNOSIS 审中-公开
    非侵入性预后诊断方法

    公开(公告)号：US20120225798A1

    公开(公告)日：2012-09-06

    申请号：US13369000

    申请日：2012-02-08

    Applicant: Charles R. Cantor ,  Chunming Ding ,  Yuk Ming Dennis Lo ,  Rossa Wai Kwum Chiu

    Inventor： Charles R. Cantor ,  Chunming Ding ,  Yuk Ming Dennis Lo ,  Rossa Wai Kwum Chiu

    IPC: C40B30/10

    CPC classification number: C12Q1/6881 ,  C12Q1/6872 ,  C12Q2600/156 ,  C12Q2600/172 ,  C12Q2535/125

    Abstract: The present invention is directed to methods of detecting nucleic acids in a biological sample. The method is based on a novel combination of a base extension reaction, which provides excellent analytical specificity, and a mass spectrometric analysis, which provides excellent specificity. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method allows accurate detection of nucleic acids that are present in very small amounts in a biological sample. For example, the method of the present invention is preferably used to detect fetal nucleic acid in a maternal blood sample; circulating tumor-specific nucleic acids in a blood, urine or stool sample; and donor-specific nucleic acids in transplant recipients. In another embodiment, one can detect viral, bacterial, fungal, or other foreign nucleic acids in a biological sample.

    Abstract translation: 本发明涉及检测生物样品中核酸的方法。 该方法基于提供优异分析特异性的碱基延伸反应和提供优异特异性的质谱分析的新型组合。 该方法可用于例如诊断，预后和治疗目的。 该方法允许精确检测在生物样品中以非常少的量存在的核酸。 例如，本发明的方法优选用于检测母体血液样品中的胎儿核酸; 在血液，尿液或粪便样品中循环肿瘤特异性核酸; 和供体特异性核酸。 在另一个实施方案中，可以检测生物样品中的病毒，细菌，真菌或其它外来核酸。

    Nucleic acid supported protein complementation
    20.
    发明授权
    Nucleic acid supported protein complementation 有权
    核酸支持的蛋白质互补

    公开(公告)号：US07999095B2

    公开(公告)日：2011-08-16

    申请号：US12638871

    申请日：2009-12-15

    Applicant: Charles R. Cantor ,  Natalia E. Broude ,  Carlos Witte-Hoffman

    Inventor： Charles R. Cantor ,  Natalia E. Broude ,  Carlos Witte-Hoffman

    IPC: C07H21/04 ,  C12Q1/68 ,  G01N33/53

    CPC classification number: C12Q1/6818 ,  C12Q1/6813 ,  C12Q2563/131 ,  C12Q2561/107

    Abstract: The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive polypeptide fragment are brought in close proximity during hybridization to a target nucleic acid, they re-associate into an active, detectable protein.

    Abstract translation: 本发明涉及用于靶核酸分子（包括DNA和RNA靶标）以及核酸类似物的体外和体内检测的新方法。 本发明基于蛋白质互补，其中两个单独的多肽是无活性的。 当两个无活性多肽片段在与靶核酸杂交期间紧密接近时，它们重新连接成活性的可检测蛋白质。

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