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25 条记录 (耗时 0.033 秒)

    INTERACTION SCREENING METHODS, SYSTEMS AND DEVICES
    17.
    发明申请
    INTERACTION SCREENING METHODS, SYSTEMS AND DEVICES 审中-公开
    交互式筛选方法，系统和设备

    公开(公告)号：US20110166027A1

    公开(公告)日：2011-07-07

    申请号：US12674374

    申请日：2008-08-21

    申请人： Michael P. Weiner

    发明人： Michael P. Weiner

    IPC分类号： C40B20/04 ,  C40B30/04 ,  C12N1/00

    CPC分类号： G01N33/5302 ,  G01N33/54366

    摘要： An interaction screening method for identifying binding moieties encapsulates candidate binding moieties in droplets with a first known moiety and a second known moiety. The candidate binding moieties are different in the different droplets. The method further comprises determining for one or more of the droplets whether the candidate binding moiety is bound to the first known moiety and/or the second known moiety. Optionally, the method further comprises segregating at least one droplet in which the candidate binding moiety is bound to the first known moiety or to the first and second known moiety.

    摘要翻译： 用于鉴定结合部分的相互作用筛选方法用第一已知部分和第二已知部分将候选结合部分包封在液滴中。 候选结合部分在不同的液滴中是不同的。 所述方法还包括确定所述液滴中的一个或多个，所述候选结合部分是否与第一已知部分和/或第二已知部分结合。 任选地，该方法进一步包括将候选结合部分与第一已知部分或第一和第二已知部分结合的至少一个液滴分离。

    Systems and methods for nucleic acid sequencing
    18.
    发明授权
    Systems and methods for nucleic acid sequencing 有权

    公开(公告)号：US09797010B2

    公开(公告)日：2017-10-24

    申请号：US12809120

    申请日：2008-12-19

    申请人： David A. Weitz ,  Jeremy Agresti ,  Michael P. Weiner ,  Adam R. Abate ,  Tony Hung

    发明人： David A. Weitz ,  Jeremy Agresti ,  Michael P. Weiner ,  Adam R. Abate ,  Tony Hung

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q2537/143 ,  C12Q2563/149

    摘要： The present invention relates to systems and methods for sequencing nucleic acids, including sequencing nucleic acids in fluidic droplets. In one set of embodiments, the method employs sequencing by hybridization using droplets such as microfluidic droplets. In some embodiments, droplets are formed which include a target nucleic acid, a nucleic acid probe, and at least one identification element, such as a fluorescent particle. The nucleic acid probes that hybridize to the target nucleic acid are determined, in some instances, by determining the at least one identification element. The nucleic acid probes that hybridize to the target nucleic acid may be used to determine the sequence of the target nucleic acid. In certain instances, the microfluidic droplets are provided with reagents that modify the nucleic acid probe. In some cases, a droplet, such as those described above, is deformed such that the components of the droplets individually pass a target area.

    Method for the directional cloning of DNA
    20.
    发明授权
    Method for the directional cloning of DNA 失效
    DNA定向克隆方法

    公开(公告)号：US5523221A

    公开(公告)日：1996-06-04

    申请号：US78662

    申请日：1993-06-16

    申请人： Michael P. Weiner

    发明人： Michael P. Weiner

    IPC分类号： C12N15/66 ,  C12N15/11

    CPC分类号： C12N15/66

    摘要： A method for directionally cloning an insert DNA fragment into a target sequence using differential phosphorylation is disclosed, Monophosphorylated PCR fragments are directionally cloned into a monophosphorylated plasmid, Methods for directionally cloning non-PCR fragments into target DNA sequences are also discussed.

    摘要翻译： 公开了使用差异磷酸化将插入DNA片段定向克隆到靶序列中的方法，单磷酸化PCR片段被定向克隆到单磷酸化质粒中。还讨论了将非PCR片段定向克隆到靶DNA序列中的方法。