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公开(公告)号：US11739044B2
公开(公告)日：2023-08-29
申请号：US17183324
申请日：2021-02-23
Inventor： Ming-Hsi Chuang , Lin-Hsiang Chuang , Ming-Hsuan Lu , Wen-Peng Shen , Yun-Chen Lin , Chi-Hsuan Chuang
Abstract: The present invention relates to a method for synthesizing artepillin C and a synthetic method for synthesizing an intermediate compound for use in preparing artepillin C. The structural formula of artepillin C is shown in the following formula (IV), and the intermediate compound is shown in the following formula (II). The synthesis method includes the following steps: a first reaction step: in the presence of a base initiator, a compound A shown in formula I and 3,3-dimethylallyl bromide are mixed, the reaction continues for a first reaction time and is stopped to generate an intermediate of artepillin C shown in formula II; and a second reaction step: the intermediate of artepillin C is dissolved in an aqueous alcohol solution and then mixed with an alkali metal salt, and the reaction continues for a second reaction time to generate artepillin C.
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12.发明公开公开(公告)号：US20230201266A1
公开(公告)日：2023-06-29
申请号：US17470487
申请日：2021-09-09
Inventor： Feng-Huei Lin , Che-Yung Kuan , Yu-Ying Lin , Ching-Yun Chen , Zhi-Yu Chen , I-Hsuan Yang , Ming-Hsi Chuang , Po-Cheng Lin , Chia-Hsin Lee , Kai-Ling Zhang , Pei-Syuan Chao , Wan-Sin Syu , Chun-Hung Chen , Ting-Ju Wang
IPC: A61K35/28 , A61P19/02 , C12N5/0775 , C12N5/00
CPC classification number: A61K35/28 , A61P19/02 , C12N5/0062 , C12N5/0667 , C12N2513/00 , C12N2533/30
Abstract: The present disclosure provides a method for treating arthritis by using a stem cell preparation. The stem cell preparation of the present disclosure can effectively delay cartilage degeneration caused by arthritis, and it is confirmed by whole blood analysis and blood biochemical analysis that the stem cell preparation in the form of three-dimensional stem cell spheres provides a safe treatment for arthritis. The present disclosure also provides a method for preparing the stem cell preparation.
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公开(公告)号：US20230193198A1
公开(公告)日：2023-06-22
申请号：US18066999
申请日：2022-12-15
Inventor： Ming-Hsi Chuang , Po-Cheng Lin , Chia-Hsin Lee , Mao-Hsuan Huang
IPC: C12N5/074
CPC classification number: C12N5/0607 , C12N2533/74 , C12N2500/98 , C12N2500/12 , C12N2500/32 , C12N2523/00
Abstract: A method for stably amplifying a pluripotent stem cell comprises the following steps: (a) a cell implantation step: implanting pluripotent stem cells directly into a porous scaffold such that the porous scaffold contains 1×104 or more of the pluripotent stem cells; and (b) a cell amplification step: immersing the porous scaffold in a specific culture medium which is xeno-free (XF) and performing amplification culture at an ambient temperature of 35.5-39.5° C. and a CO2 concentration of 5% to obtain the amplified pluripotent stem cells, wherein the amplified pluripotent stem cells aggregate to present an embryoid body state. The amplification method of the present disclosure can easily obtain an excellent effect of increasing an amplification multiple of the pluripotent stem cells to about 3 times or more.
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公开(公告)号：US20220098139A1
公开(公告)日：2022-03-31
申请号：US17183324
申请日：2021-02-23
Inventor： Ming-Hsi Chuang , Lin-Hsiang Chuang , Ming-Hsuan Lu , Wen-Peng Shen , Yun-Chen Lin , Chi-Hsuan Chuang
Abstract: The present invention relates to a method for synthesizing artepillin C and a synthetic method for synthesizing an intermediate compound for use in preparing artepillin C. The structural formula of artepillin C is shown in the following formula (IV), and the intermediate compound is shown in the following formula (II). The synthesis method includes the following steps: a first reaction step: in the presence of a base initiator, a compound A shown in formula I and 3,3-dimethylallyl bromide are mixed, the reaction continues for a first reaction time and is stopped to generate an intermediate of artepillin C shown in formula II; and a second reaction step: the intermediate of artepillin C is dissolved in an aqueous alcohol solution and then mixed with an alkali metal salt, and the reaction continues for a second reaction time to generate artepillin C.
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公开(公告)号：US20150274923A1
公开(公告)日：2015-10-01
申请号：US14632730
申请日：2015-02-26
Inventor： Jui-Yang Lai , Kao-Yuan Chang , Zih-Han Hong , Po-Cheng Lin , Ming-Hsi Chuang
CPC classification number: C08J9/36 , A61K9/5031 , A61K35/28 , A61K35/35 , A61L27/16 , A61L27/18 , A61L27/22 , A61L27/50 , A61L27/56 , A61L27/58 , A61L2300/222 , A61L2300/236 , A61L2300/25 , A61L2300/252 , A61L2300/64 , A61L2430/28 , C08J2207/10 , C08J2367/04 , C08J2405/08 , C08L67/04 , C08L29/04
Abstract: A method for manufacturing a hollow particle is provided. The method comprises the steps of (a) providing a hollow particulate; (b) soaking the hollow particulate in an amine solution to form amine groups on the surface of the hollow particulate; (c) adding a polypeptide, and the polypeptide is linked to the amine groups on the surface of the hollow particulate; and (d) adding a target molecule, and the target molecule is bound to the amine group which are still not bound.
Abstract translation: 提供一种制造中空颗粒的方法。 该方法包括以下步骤：（a）提供中空颗粒; （b）将中空颗粒浸泡在胺溶液中以在中空颗粒的表面上形成胺基; （c）加入多肽，并且多肽与中空颗粒表面上的胺基连接; 和（d）添加目标分子，并且靶分子与尚未结合的胺基结合。
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Patent Agency Ranking
公开(公告)号：US20150238534A1
公开(公告)日：2015-08-27
申请号：US14632760
申请日：2015-02-26
Inventor： Jui-Yang Lai , Li-Jyuan Luo , Zih-Han Hong , Kao-Yuan Chang , Po-Cheng Lin , Ming-Hsi Chuang
CPC classification number: C08J9/36 , A61K9/5031 , A61K35/28 , A61K35/35 , A61L27/16 , A61L27/18 , A61L27/22 , A61L27/50 , A61L27/56 , A61L27/58 , A61L2300/222 , A61L2300/236 , A61L2300/25 , A61L2300/252 , A61L2300/64 , A61L2430/28 , C08J2207/10 , C08J2367/04 , C08J2405/08 , C08L67/04 , C08L29/04
Abstract: A hollow particle is provided. The hollow particle of the invention comprises (a) a hollow microparticle, (b) a plural of linking molecules binding the surface of the microparticle, (c) a polypeptide binding the linking molecule, and (d) a target molecule binding the linking molecule. The hollow microparticle of the invention can deliver more cells and provides a higher rate of cell transplantation by the hollow structures, polypeptides and target molecules. The present invention also provides a manufacture of the hollow microparticle and a cell carrier.
Abstract translation: 提供中空颗粒。 本发明的中空颗粒包含（a）中空微粒，（b）多个结合微粒表面的连接分子，（c）与结合分子结合的多肽，和（d）结合连接分子的靶分子 。 本发明的中空微粒可以递送更多的细胞并通过中空结构，多肽和靶分子提供更高的细胞移植速率。 本发明还提供了中空微粒和电池载体的制造。
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公开(公告)号：US20240124844A1
公开(公告)日：2024-04-18
申请号：US18376459
申请日：2023-10-04
Inventor： Chia-Hsin Lee , Po-Cheng Lin , Yong-Cheng Kao , Ming-Hsi Chuang , Chun-Hung Chen , Chao-Liang Chang , Kai-Ling Zhang
IPC: C12N5/0775 , A61P19/02
CPC classification number: C12N5/0667 , A61P19/02 , C12N2500/32 , C12N2500/38 , C12N2501/10
Abstract: The present disclosure provides a method for preparing a composition including mesenchymal stem cells, extracellular vesicles produced by the mesenchymal stem cells, and growth factors, the composition prepared by the method, and use of the composition for treating arthritis. The composition of the present disclosure achieves the effect of treating arthritis through various efficacy experiments.
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公开(公告)号：US20230233615A1
公开(公告)日：2023-07-27
申请号：US18097509
申请日：2023-01-17
Inventor： Chi-Ying Huang , Yu-Chen Tsai , Ming-Hsi Chuang , Po-Cheng Lin
IPC: A61K35/28 , A61P25/28 , C12N15/113 , C12N9/64 , C12N5/0775
CPC classification number: A61K35/28 , A61P25/28 , C12N5/0667 , C12N9/6494 , C12N15/113 , C12Y304/24011 , C12N2310/141 , C12N2310/531 , C12N2500/32 , C12N2500/42
Abstract: The present disclosure provides a pharmaceutical composition for treating Alzheimer’s disease, which at least includes an extracellular vesicle that is prepared by a method including: a first culturing step: performing an amplification culture of an adipose-derived stem cell in a first culture medium at a cell density of 6,000-15,000 cells/cm2 until an amplification amount of the adipose-derived stem cell is above 90% of that before the culture; a second culturing step: culturing the amplified adipose-derived stem cell in a second culture medium at a cell density of 10,000-100,000 cells/cm2 for 20-30 hours; and an extracellular vesicle separating step: collecting a culture solution and separating the extracellular vesicle from the culture solution by utilizing a tangential flow filtration (TFF) or ultrafiltration method.
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公开(公告)号：US20190136195A1
公开(公告)日：2019-05-09
申请号：US16237399
申请日：2018-12-31
Inventor： Shao-Chih Chiu , Wan-Sin Syu , Ming-Hsi Chuang , Po-Cheng Lin
IPC: C12N5/0775 , A61K35/28
Abstract: A composition for treatment of tendonitis is provided. The composition comprises a pretreated adipose derived stem cell (ADSC), wherein the ADSC is pretreated with butylidenephthalide, and the concentration of butylidenephthalide is greater or equal to. The composition of the invention has abilities to repair damaged tendon fiber, enhance tissue regeneration, and decrease inflammation. The invention also provides a method for manufacturing a composition for treatment of tendonitis, comprising culturing an ADSC in a medium containing butylidenephthalide.
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公开(公告)号：US20180127722A1
公开(公告)日：2018-05-10
申请号：US15416502
申请日：2017-01-26
Inventor： Shao-Chih Chiu , Wan-Sin Syu , Ming-Hsi Chuang , Po-Cheng Lin
IPC: C12N5/0775 , A61K35/28
CPC classification number: C12N5/0667 , A61K35/28 , C12N2500/30 , C12N2500/76
Abstract: A composition for treatment of tendonitis is provided. The composition comprises a pretreated adipose derived stem cell (ADSC), wherein the ADSC is pretreated with butylidenephthalide, and the concentration of butylidenephthalide is greater or equal to. The composition of the invention has abilities to repair damaged tendon fiber, enhance tissue regeneration, and decrease inflammation. The invention also provides a method for manufacturing a composition for treatment of tendonitis, comprising culturing an ADSC in a medium containing butylidenephthalide.
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