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35 条记录 (耗时 0.026 秒)

    Method of DNA array construction, gene expression analysis, and discovery of useful genes
    15.
    发明申请
    Method of DNA array construction, gene expression analysis, and discovery of useful genes 审中-公开
    DNA阵列构建方法，基因表达分析和有用基因的发现

    公开(公告)号：US20050176036A1

    公开(公告)日：2005-08-11

    申请号：US11008275

    申请日：2004-12-10

    申请人： Takahide Yokoi ,  Takashi Minowa ,  Toshiro Saito ,  Yoshiko Kaku

    发明人： Takahide Yokoi ,  Takashi Minowa ,  Toshiro Saito ,  Yoshiko Kaku

    IPC分类号： G01N33/53 ,  C12M1/00 ,  C12M1/34 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N37/00 ,  B05D3/00

    CPC分类号： C12Q1/6837 ,  C12Q2600/158 ,  C12Q2525/179

    摘要： Methods for global monitoring of gene expression and searching for useful genes that are targeted to organisms lacking sequence information are realized by providing low-cost and efficient DNA arrays. A genomic library constructed from randomly cleaved genomic DNA fragments is directly fixed on a substrate that allows the library to be individually recognized. In this way, global monitoring of gene expression can be carried out without being limited by the amount of gene sequence information. Further, plasmids that are detected by a constructed random genomic DNA array are fragmented into shorter DNAs, which are fixed on another substrate to construct a sub DNA array. With the use of this sub DNA array, gene expression is analyzed and useful genes are searched.

    摘要翻译： 通过提供低成本和有效的DNA阵列来实现全球监测基因表达和寻找靶向缺乏序列信息的生物体的有用基因的方法。 由随机切割的基因组DNA片段构建的基因组文库直接固定在允许文库单独识别的底物上。 以这种方式，可以在不受基因序列信息量的限制的情况下进行基因表达的全局监测。 此外，通过构建的随机基因组DNA阵列检测的质粒被片段化成较短的DNA，其被固定在另一底物上以构建亚DNA阵列。 通过使用该DNA DNA阵列，分析基因表达，并搜索有用的基因。

    Recording/reproducing method and recording/reproducing apparatus
    16.
    发明授权
    Recording/reproducing method and recording/reproducing apparatus 失效
    记录/再现方法和记录/再现装置

    公开(公告)号：US5898607A

    公开(公告)日：1999-04-27

    申请号：US866307

    申请日：1997-05-30

    申请人： Toshiro Saito ,  Shigeo Suzuki ,  Masato Isogai

    发明人： Toshiro Saito ,  Shigeo Suzuki ,  Masato Isogai

    IPC分类号： G11B11/08 ,  G11B13/00 ,  G11C13/00

    CPC分类号： G11B11/08 ,  G11B13/00

    摘要： A voltage of an intensity corresponding to information to be recorded is applied to a recording medium which comprises a film containing a charge generating material. The film is sandwiched between two electrodes. Electric charges are accumulated in the recording medium upon application of the voltage so that information is recorded. When light is irradiated to the recording medium, the stored charges are converted into photo-current. The recorded information is read out by detecting the photo-current. When the electrodes are short-circuited, the recorded information is erased.

    摘要翻译： 对应于要记录的信息的强度的电压被施加到包括含有电荷产生材料的膜的记录介质。 该膜夹在两个电极之间。 在施加电压时，电荷积累在记录介质中，从而记录信息。 当光照射到记录介质时，所存储的电荷被转换为光电流。 通过检测光电流来读出记录的信息。 当电极短路时，记录的信息被擦除。

    METHOD AND APPARATUS FOR NUCLEIC ACID ANALYSIS
    19.
    发明申请
    METHOD AND APPARATUS FOR NUCLEIC ACID ANALYSIS 审中-公开
    核酸分析方法与装置

    公开(公告)号：US20140200162A1

    公开(公告)日：2014-07-17

    申请号：US14233256

    申请日：2012-05-16

    申请人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

    发明人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

    IPC分类号： C12Q1/68 ,  C12N15/10

    CPC分类号： C12Q1/6834 ,  C12N15/1065 ,  C12Q2525/207 ,  C12Q2537/125 ,  C12Q2537/143 ,  C12Q2563/143 ,  C12Q2563/149

    摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.

    摘要翻译： 提供了一种方便的核酸分析方法，使得能够以高全面性和至少四位数的动态范围共同分析1000种或更多种类型的核酸。 特别地，提供了非常有效的分析方法，特别是对于非翻译的RNA和微小RNA，其靶核酸的类型为10000或更低。 可以通过以下步骤以单分子灵敏度和分辨率的高全面性和定量性能方便快速地分析核酸：一次一个分子制备一组靶核酸片段，并将已知的核酸分子杂交 碱基序列，并用荧光物质标记，用靶核酸片段组来检测标记杂交核酸分子的荧光物质。

    Method for evaluating DNA probes position on substrate
    20.
    发明申请
    Method for evaluating DNA probes position on substrate 失效
    评估DNA探针在底物上的位置的方法

    公开(公告)号：US20050208561A1

    公开(公告)日：2005-09-22

    申请号：US11113195

    申请日：2005-04-25

    申请人： Kazuhito Rokutan ,  Hiroyuki Tomita ,  Toshiro Saito

    发明人： Kazuhito Rokutan ,  Hiroyuki Tomita ,  Toshiro Saito

    IPC分类号： G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N37/00 ,  C12M1/34

    CPC分类号： G06F19/20 ,  C12Q1/6883 ,  C12Q2565/513 ,  C12Q2600/158 ,  C12Q2600/166

    摘要： An oligonucleotide array comprising an array of multiple oligonucleotides with different base sequences fixed onto known and separate positions on a support substrate, wherein said oligonucleotides are biological stress related genes or complementary sequence chains to the said genes, and the said oligonucleotides are classified according to their gene functions, wherein the fixation region on the support substrate is divided into the said classification.

    摘要翻译： 一种寡核苷酸阵列，其包含具有不同碱基序列的多个寡核苷酸阵列，其固定在载体底物上的已知和分开的位置上，其中所述寡核苷酸是与所述基因相关的生物应激相关基因或互补序列链，并且所述寡核苷酸根据它们 基因功能，其中支撑衬底上的固定区域被划分为所述分类。