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11.发明授权Automated analyzer for screening cells or formed bodies for enumeration of populations expressing selected characteristics 失效用于筛选细胞或成体的自动分析仪，用于计数表达所选特征的群体
公开(公告)号：US5464752A
公开(公告)日：1995-11-07
申请号：US046404
申请日：1993-04-08
IPC分类号： G01N15/10 , G01N15/12 , G01N15/14 , G01N33/50 , G01N33/53 , G01N33/543 , G01N33/569 , G01N35/00 , C12Q1/24 , G01N33/577
CPC分类号： G01N33/5094 , G01N33/5302 , G01N33/54326 , G01N33/56972 , G01N15/12 , G01N15/14 , G01N2015/1037 , G01N2015/1062 , G01N2015/1081 , G01N35/0098 , Y10S436/808
摘要： A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected white blood cell population and/or subset thereof of a whole blood sample or portion thereof. A volume of a biological sample containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population analysis.The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.
摘要翻译： 一种用于自动并快速地检索，计数和/或分析全血样本或其部分的至少一个所选择的白细胞群体和/或其子集的方法和装置。 制备含有白细胞的体积的生物样品，并将至少一种特异性或优先选择的至少一些选定的生物细胞引入其中并在短时间内快速混合。 可以修改细胞的不透明度和/或体积参数，然后在一个或多个步骤中对混合物进行计数和分析以获得所需的白细胞群体分析。 生物样品可以是全血样品，并且反应物可以包括结合微球的裂解液或单克隆抗体，其将与特定细胞结合，或与结合抗体的溶血和微球体的组合。 微球可以是磁性的，并且结合的细胞可被磁性去除，用于检索和分析剩余的血细胞群体。
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公开(公告)号：US5653686A
公开(公告)日：1997-08-05
申请号：US372196
申请日：1995-01-13
CPC分类号： G01N35/1079 , A61J1/2089 , B01L3/5082 , A61J1/201 , A61J1/2017 , A61J1/2058 , A61J1/2072 , A61J1/2075 , A61J1/2082 , A61J1/2086 , A61J1/2096
摘要： A method and transfer device for transferring fluids between closed vials. The transfer device includes a cylinder having a pair of opposing open tubes or tubular portions into which a pair of vials can be inserted to transfer fluid therebetween. The tubes are separated by a wall through which extends a fluid transfer needle and a vent needle which first is inserted into a first vial to vent the first vial into a vent chamber or reservoir formed in the transfer device before the transfer needle is inserted into the first vial. The first vial, stopper first, is inserted into first tube until seated onto an exposed end of the transfer needle. A second vial, stopper first, then is inserted into the second tube until seated onto the opposite exposed end of the transfer needle. A pressure differential between the closed vials preferably can be utilized to transfer a desired fluid portion between the vials.
摘要翻译： 一种用于在密封小瓶之间传送流体的方法和传送装置。 转移装置包括具有一对相对的开放管或管状部分的圆柱体，一对小瓶可插入其中以在其间传送流体。 管被壁分开，通过该壁将流体输送针和通气针延伸，首先将其插入第一小瓶中，以将第一小瓶排出到形成在转移装置中的通气室或储存器中，然后将输送针插入 第一个小瓶。 首先将第一个小瓶，塞子插入第一个管子，直到坐到转移针的暴露端上。 首先将第二个小瓶，塞子插入第二个管子中，直到安置在转移针的相对的暴露端上。 密封小瓶之间的压力差优选地可用于在小瓶之间转移期望的流体部分。
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13.发明授权Particle analyzer of the Coulter type including coincidence error correction circuitry 失效Coulter型粒子分析仪包括重合误差校正电路
公开(公告)号：US3968429A
公开(公告)日：1976-07-06
申请号：US594209
申请日：1975-07-09
申请人： Wallace H. Coulter , Walter R. Hogg
发明人： Wallace H. Coulter , Walter R. Hogg
CPC分类号： G01N15/1245 , G01N15/1227
摘要： A particle analyzer of the Coulter type has at least first and second sensing zone arrangements, each having substantially the same dimensions and each generating a train of particle pulses in response to passage through the respective sensing zone of a sample containing a plurality of particles to be counted. A summing circuit connected to the output of each sensing zone sums together each of the trains of particle pulses to develop a summed train of pulses. Additional circuitry is connected to each sensing zone and the summing circuitry. This circuitry is operative to change mathematically at least the number of particle pulses in the summed train of particle pulses. The mathematically changed summed train of pulses and the train of pulses from the first and second sensing zones then are accumulated to produce an error corrected particle pulse count.
摘要翻译： 库尔特型的粒子分析仪具有至少第一和第二感测区域布置，每个具有基本上相同的尺寸，并且每个产生一系列粒子脉冲以响应于通过包含多个颗粒的样品的相应感测区域而成 算了 连接到每个感测区域的输出的求和电路将每个粒子脉冲列组合在一起，以产生一个总和的脉冲序列。 附加电路连接到每个感测区域和求和电路。 该电路用于在数学上改变粒子脉冲的总和列中的至少粒子脉冲的数量。 然后累积数学改变的脉冲序列和来自第一和第二感测区域的脉冲串，以产生误差校正的粒子脉冲计数。
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14.发明授权公开(公告)号：US06900029B1
公开(公告)日：2005-05-31
申请号：US08556667
申请日：1995-11-13
IPC分类号： B01D21/00 , G01N33/543 , G01N33/553 , G01N33/569 , G01N33/574 , A01N1/02 , C12Q1/18 , G01N33/53
CPC分类号： G01N33/54333 , G01N33/553 , G01N33/56972 , G01N33/574 , Y10S436/824
摘要： A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including an enriched population is separated from the particles with the selected population bound thereto. The enriched populations in the biological sample supernatant can be further enriched by multiple removal steps.
摘要翻译： 用于使用相对重的，致密的颗粒和重力沉降从生物样品中分离所选择的所需或不需要的群体的分离程序。 颗粒具有一个或多个与其结合的反应物，其特异于并将与所选群体结合。 颗粒优选通过重复使颗粒沉淀通过样品的大部分与样品结合而与样品混合。 然后允许具有结合的所选群体的颗粒优先沉降在样品中，并且将包含富集群体的上清液从结合有所选群体的颗粒中分离。 通过多次去除步骤，可以进一步富集生物样品上清液中的富集群体。
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15.发明授权
公开(公告)号：US3936740A
公开(公告)日：1976-02-03
申请号：US442110
申请日：1974-02-13
申请人： Walter R. Hogg , Wallace H. Coulter
发明人： Walter R. Hogg , Wallace H. Coulter
CPC分类号： H03K5/14
摘要： An apparatus which includes circuitry for automatically sampling each pulse in a series of pulses a predetermined average number of times, sequentially storing the samples for a period of time, and reproducing the pulses from the samples, delayed in time.A method of operation is encompassed within the invention as well as use of the above apparatus in a particle study device.
摘要翻译： 一种装置，其包括用于以预定的平均次数自动采样一系列脉冲中的每个脉冲的电路，顺序地将样本存储一段时间，并且在时间上延迟地从样本中再现脉冲。
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16.发明授权Method and apparatus for transporting carriers of sealed sample tubes and mixing the samples 失效运送密封样品管的载体并混合样品的方法和装置
公开(公告)号：US4609017A
公开(公告)日：1986-09-02
申请号：US541603
申请日：1983-10-13
IPC分类号： G01N33/48 , G01N1/00 , G01N1/10 , G01N33/483 , G01N35/00 , G01N35/02 , G01N35/04 , G01N35/10 , G01N1/14
CPC分类号： G01N35/1079 , G01N35/0095 , G01N2035/00524 , G01N2035/041 , G01N2035/0415
摘要： A plurality of sealed blood sample tubes are housed in a rack and a plurality of these racks are vertically stacked, with the sample tubes lying horizontally. The racks are successively deposited onto a horizontal conveyor belt which is housed in and moves longitudinally on a table that rocks around its longitudinal axis to mix the samples in a semi-inverting mode as a rack is stepped from the stack to a sample aspiration station and/or thereat. The aspiration station preferably includes a sample segmenting and diluting valve and a sample identification reader. Aspiration is accomplished by pushing a sample tube partially out from the carrier rack and onto a seal piercing tip of the aspiration probe. The tube then is returned to the rack. The other tubes in that rack similarly are aspirated after the rack is stepped to align each tube with the aspiration probe, with table rocking and sampling mixing accompanying each advancing step of the rack. After a rack has advanced through the aspiration station, it is stacked out of the way.
摘要翻译： 将多个密封的血液样品管容纳在齿条中，并且这些支架的多个垂直地堆叠，其中试样管水平放置。 机架依次沉积在水平的传送带上，水平的传送带被容纳在纵向移动的桌子上并且纵向移动到桌子上，桌子围绕其纵向轴线摇摆，以便在从堆叠步进到样品抽吸站的半倒置模式下混合样品， /或其中 抽吸站优选包括样品分段和稀释阀以及样品识别读取器。 通过将样品管部分地从载体架推出并到吸入探针的密封穿刺尖端来实现吸气。 然后将管返回到机架。 机架上的另一个管子类似地被抽出，在机架逐步将每个管与抽吸探针对齐后，伴随着架子的每个前进步骤的桌面摆动和取样混合。 在机架通过吸气站前进后，它被堆叠在一起。
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17.发明授权Method and apparatus for providing primary coincidence correction during particle analysis utilizing time generation techniques 失效在使用时间生成技术的粒子分析期间提供主要重合校正的方法和装置
公开(公告)号：US4009443A
公开(公告)日：1977-02-22
申请号：US564722
申请日：1975-04-03
申请人： Wallace H. Coulter , Walter R. Hogg , Millard D. Longman, Jr. , Stephen Campbell , Edward Neal Doty
发明人： Wallace H. Coulter , Walter R. Hogg , Millard D. Longman, Jr. , Stephen Campbell , Edward Neal Doty
CPC分类号： H03K21/02 , G01N15/1227
摘要： A method and apparatus for correcting a particle pulse count subject to coincidence error is disclosed wherein particle pulses, developed in response to passage of particles in a particulate system through a sensing zone, are counted for a predetermined period of time. The predetermined period of time is increased or extended in response to each pulse counted by a time increment that is related to a characteristic of the counted pulse, such as the pulse width, duration, or amplitude. The total additional time period allows the counting of additional particle pulses such that the total count at the end of the extended time period is an error corrected count for the number of particles detected in the predetermined time period.
摘要翻译： 公开了一种用于校正受到重合误差的粒子脉冲计数的方法和装置，其中响应于颗粒系统中的颗粒通过感测区域而产生的颗粒脉冲被计数预定时间段。 响应于与计数脉冲的特性（例如脉冲宽度，持续时间或幅度）相关的时间增量计数的每个脉冲，预定时间段被增加或延长。 总的附加时间段允许对附加粒子脉冲进行计数，使得延长时间段结束时的总计数是在预定时间段内检测到的粒子数目的误差校正计数。
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18.发明授权Method and apparatus for providing primary coincidence correction during particle analysis utilizing time generation techniques 失效在使用时间生成技术的粒子分析期间提供主要重合校正的方法和装置
公开(公告)号：US3936741A
公开(公告)日：1976-02-03
申请号：US485160
申请日：1974-07-02
CPC分类号： H03K21/02 , G01N15/1227
摘要： A method and apparatus for correcting a particle pulse count subject to coincidence error is disclosed wherein particle pulses, developed in response to passage of particles in a particulate system through a sensing zone, are counted for a predetermined period of time. The predetermined period of time is increased or extended in response to each pulse counted by a time increment that is a function of a characteristic of the counted pulse, such as the pulse width, duration, or amplitude. The total additional time period allows the counting of additional particle pulses such that the total count at the end of the extended time period is an error corrected count for the number of particles detected in the predetermined time period.
摘要翻译： 公开了一种用于校正受到重合误差的粒子脉冲计数的方法和装置，其中响应于颗粒系统中的颗粒通过感测区域而产生的颗粒脉冲被计数预定时间段。 响应于作为脉冲宽度，持续时间或幅度等计数脉冲的特性的函数的时间增量而计数的每个脉冲，预定时间段被增加或延长。 总的附加时间段允许对附加粒子脉冲进行计数，使得延长时间段结束时的总计数是在预定时间段内检测到的粒子数目的误差校正计数。
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公开(公告)号：US4527114A
公开(公告)日：1985-07-02
申请号：US352438
申请日：1982-02-25
申请人： Wallace H. Coulter
发明人： Wallace H. Coulter
CPC分类号： G01N15/12 , G01N2015/1254
摘要： A particle analyzer apparatus comprising a flow cell having a flow chamber wherein a flow of liquid suspension, having individual particles entrained therein, proceeds along a predetermined path; a pair of electrodes are disposed on opposed sides of the predetermined path, one of the electrodes having an end with a width parallel to the predetermined path that is less than the length of a given particle, the end of the electrode being positioned in close proximity to the predetermined path; energizing source for providing an electrical field between the pair of electrodes that traverses the predetermined path; and a particle pulse detector for detecting particle pulses caused by the particles passing through the electric field.
摘要翻译： 一种颗粒分析仪装置，包括具有流动室的流动池，其中夹带有单个颗粒的液体悬浮液流沿预定路径进行; 一对电极设置在预定路径的相对侧上，其中一个电极具有平行于预定路径的宽度小于给定粒子的长度的端部，电极的端部紧邻 到预定路径; 激励源，用于在穿过所述预定路径的所述一对电极之间提供电场; 以及用于检测由通过电场的粒子引起的粒子脉冲的粒子脉冲检测器。
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公开(公告)号：US4326851A
公开(公告)日：1982-04-27
申请号：US200142
申请日：1980-10-24
CPC分类号： G01N35/1009 , G01F23/265 , G01N2035/1025 , G01N2035/1086 , G01N35/1004
摘要： A level sensor of a fluid transfer mechanism for determining when the bottom tip of a fluid aspirating probe touches or contacts the top surface of a sample fluid. The level sensor senses the contact capacitively by grounding one plate of a capacitor through the sample fluid and fluid aspirating probe. One capacitor plate is formed of an electrode biased against the bottom of a rotatable supply tray and positioned under a sample containing cavity in the tray. The other capacitor plate is formed by the bottom surface of the sample fluid in the supply cavity, and the dielectric of the capacitor is formed of the supply tray material. An AC signal is applied to the electrode and the probe is grounded. The change in voltage occurring in the AC signal at the electrode when the probe touches the top surface of the sample fluid it is sensed to provide the touch signal.
摘要翻译： 流体传送机构的液位传感器用于确定流体抽吸探针的底部尖端何时接触或接触样品流体的顶表面。 电平传感器通过将样品流体和流体抽吸探头的电容器的一个接地板接地来感测接触电容。 一个电容器板由电极偏置在可旋转的供给托盘的底部并且位于托盘内的样品容纳腔的下方形成。 另一个电容器板由供给腔中的样品流体的底表面形成，并且电容器的电介质由供给盘材料形成。 将AC信号施加到电极并且探针接地。 当探针接触样品流体的顶表面时，在电极处的AC信号中发生的电压的变化被感测以提供触摸信号。
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