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    APPARATUS AND METHODS FOR PERFORMING ELECTROCHEMICAL REACTIONS
    12.
    发明申请
    APPARATUS AND METHODS FOR PERFORMING ELECTROCHEMICAL REACTIONS 审中-公开
    用于执行电化学反应的装置和方法

    公开(公告)号：US20140202883A1

    公开(公告)日：2014-07-24

    申请号：US14162612

    申请日：2014-01-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John NOBILE ,  George T. ROTH ,  Todd REARICK ,  Jonathan M. SCHULTZ ,  Jonathan ROTHBERG ,  David MARRAN

    IPC: G01N27/30

    CPC classification number: G01N27/301 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/005 ,  B01J2219/00653 ,  B01J2219/00722 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  G01N27/4145

    Abstract: The invention is directed to apparatus and methods for delivering multiple reagents to, and monitoring, a plurality of analytical reactions carried out on a large-scale array of electronic sensors under minimal noise conditions. In one aspect, the invention provides method of improving signal-to-noise ratios of output signals from the electronic sensors sensing analytes or reaction byproducts by subtracting an average of output signals measured from neighboring sensors where analyte or reaction byproducts are absent. In other aspects, the invention provides an array of electronic sensors integrated with a microwell array for confining analytes and/or particles for analytical reactions and a method for identifying microwells containing analytes and/or particles by passing a sensor-active reagent over the array and correlating sensor response times to the presence or absence of analytes or particles. Such detection of analyte- or particle-containing microwells may be used as a step in additional noise reduction methods.

    Abstract translation: 本发明涉及用于在最小噪声条件下向多个电子传感器阵列上进行的多个分析反应的多个试剂的输送和监测的装置和方法。 一方面，本发明提供了通过减去从相邻传感器测得的输出信号的平均值来提高来自电子传感器感测分析物或反应副产物的输出信号的信噪比的方法，其中分析物或反应副产物不存在。 在其它方面，本发明提供了与微孔阵列集成的电子传感器阵列，用于限制用于分析反应的分析物和/或颗粒，以及通过使传感器活性试剂通过阵列而识别含有分析物和/或颗粒的微孔的方法，以及 将传感器响应时间与分析物或颗粒的存在或不存在相关联。 含有分析物或含微粒微孔的这种检测可以用作额外的降噪方法中的步骤。

    SYSTEM AND METHOD FOR SEQUENCING
    13.
    发明申请
    SYSTEM AND METHOD FOR SEQUENCING 有权

    公开(公告)号：US20210054450A1

    公开(公告)日：2021-02-25

    申请号：US16994318

    申请日：2020-08-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark REED ,  Chiu Tai Andrew WONG ,  David MARRAN

    IPC: C12Q1/6874

    Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.

    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS
    14.
    发明申请
    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS 审中-公开

    公开(公告)号：US20160011145A1

    公开(公告)日：2016-01-14

    申请号：US14863718

    申请日：2015-09-24

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan M. ROTHBERG ,  James BUSTILLO ,  Mark James MILGREW ,  Jonathan SCHULTZ ,  David MARRAN ,  Todd REARICK ,  Kim L. JOHNSON

    IPC: G01N27/414 ,  C12Q1/68

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

    Abstract: The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. In one embodiment, the characteristic of said chemical or biological sample is a concentration of a charged species and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating gate with a dielectric layer on a surface thereof, the dielectric layer contacting said sample fluid and being capable of accumulating charge in proportion to a concentration of the charged species in said sample fluid. In one embodiment such charged species is a hydrogen ion such that the sensors measure changes in pH of the sample fluid in or adjacent to the sample-retaining region thereof. Apparatus and chips of the invention may be adapted for large scale pH-based DNA sequencing and other bioscience and biomedical applications.

    Systems and Methods to Control Dissolved Gas
    15.
    发明申请
    Systems and Methods to Control Dissolved Gas 审中-公开
    控制溶解气体的系统和方法

    公开(公告)号：US20150252406A1

    公开(公告)日：2015-09-10

    申请号：US14721916

    申请日：2015-05-26

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： James A. BALL ,  David KUPEC ,  David MARRAN ,  Jon A. HOSHIZAKI

    IPC: C12Q1/68 ,  G01N27/414

    CPC classification number: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6883 ,  G01N27/4141 ,  Y10T436/143333 ,  C12Q2527/119 ,  C12Q2565/607

    Abstract: A method of sensing nucleotide reactions includes flowing at least one nucleotide solution from a container of at least two containers of a sensor system. The sensor system includes a sensor sensitive to a byproduct of nucleotide incorporation. Each container of the at least two containers includes a different nucleotide solution. The sensor system enters an idle mode after flowing. The method further includes cycling the at least two containers through at least two cycles. Each cycle includes depressurizing the at least two containers for a first period and pressurizing the at least two containers for a second period. The method also includes pressurizing the at least two containers when the sensor system enters an active mode.

    Abstract translation: 感测核苷酸反应的方法包括从传感器系统的至少两个容器的容器流出至少一个核苷酸溶液。 传感器系统包括对核苷酸掺入的副产物敏感的传感器。 至少两个容器的每个容器都包含不同的核苷酸解决方案。 传感器系统在流动后进入空闲模式。 该方法还包括使至少两个容器循环至少两个循环。 每个循环包括使至少两个容器减压第一时间段，并且将至少两个容器加压第二周期。 该方法还包括当传感器系统进入活动模式时对至少两个容器进行加压。

    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS
    16.
    发明申请
    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS 审中-公开
    用于生物和化学分析的集成传感器阵列

    公开(公告)号：US20150197797A1

    公开(公告)日：2015-07-16

    申请号：US14599948

    申请日：2015-01-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan M. ROTHBERG ,  James BUSTILLO ,  Mark James MILGREW ,  Jonathan SCHULTZ ,  David MARRAN ,  Todd REARICK ,  Kim L. JOHNSON

    IPC: C12Q1/68 ,  G01N27/414

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

    Abstract: An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. The transistor array has a pitch of 10 μm or less and a sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.

    Abstract translation: 公开了一种在电路支撑衬底中包括化学场效应晶体管阵列的装置。 晶体管阵列在其表面上设置有能够从样品流体中保留化学或生物样品的样品保持区阵列。 晶体管阵列具有10μm或更小的间距，并且样品保持区位于至少一个化学场效应晶体管上，该化学场效应晶体管被配置为产生与该样品中的化学或生物样品的特征相关的至少一个输出信号 - 区域。

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