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    MODELS FOR ANALYZING DATA FROM SEQUENCING-BY-SYNTHESIS OPERATIONS
    14.
    发明申请
    MODELS FOR ANALYZING DATA FROM SEQUENCING-BY-SYNTHESIS OPERATIONS 有权

    公开(公告)号：US20220383983A1

    公开(公告)日：2022-12-01

    申请号：US17831648

    申请日：2022-06-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Todd REARICK ,  Jonathan SCHULTZ

    IPC: G16B40/10 ,  G16B40/00 ,  C12Q1/6869

    Abstract: Mathematical models for the analysis of signal data generated by sequencing of a polynucleotide strand using a pH-based method of detecting nucleotide incorporation(s). In an embodiment, the measured output signal from the reaction confinement region of a reactor array is mathematically modeled. The output signal may be modeled as a linear combination of one or more signal components, including a background signal component. This model is solved to determine the nucleotide incorporation signal. In another embodiment, the incorporation signal from the reaction confinement region of a reactor array is mathematically modeled.

    APPARATUSES, METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR FLUID POTENTIAL ARTIFACT CORRECTION IN REAGENT DELIVERY SYSTEMS
    15.
    发明申请
    APPARATUSES, METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR FLUID POTENTIAL ARTIFACT CORRECTION IN REAGENT DELIVERY SYSTEMS 有权

    公开(公告)号：US20220146450A1

    公开(公告)日：2022-05-12

    申请号：US17499676

    申请日：2021-10-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chiu Tai Andrew WONG ,  Todd REARICK ,  John DONOHUE

    IPC: G01N27/414 ,  G16B30/00

    Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

    METHODS AND APPARATUS FOR MEASURING ANALYTES
    17.
    发明申请
    METHODS AND APPARATUS FOR MEASURING ANALYTES 审中-公开
    测量分析仪的方法和装置

    公开(公告)号：US20160187289A1

    公开(公告)日：2016-06-30

    申请号：US14926264

    申请日：2015-10-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Todd REARICK ,  Mark Beauchemin

    IPC: G01N27/414 ,  C12Q1/68 ,  G01N27/27

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  G01N27/27 ,  G01N27/4148 ,  G16B30/00

    Abstract: A method is provided to sample a sensor array. The method can include measuring a waveform associated with a chemical event occurring on the sensor array. The waveform can include at least one region associated with expected measured values and at least one region associated with unpredictable measured values. The method can also include applying a first frame averaging to the at least one region associated with the expected measured values. Here, a first number of frames can be included in the first frame averaging. Further, the method can include applying a second frame averaging to the at least one region associated with the unpredictable measured values, where a second number of frames can be included in the second frame averaging. The second number of frames can be less than the first number of frames.

    Abstract translation: 提供了一种对传感器阵列进行采样的方法。 该方法可以包括测量与传感器阵列上发生的化学事件相关联的波形。 波形可以包括与预期测量值相关联的至少一个区域和与不可预测的测量值相关联的至少一个区域。 该方法还可以包括将第一帧平均应用于与预期测量值相关联的至少一个区域。 这里，可以在第一帧平均中包括第一数量的帧。 此外，该方法可以包括将第二帧平均应用于与不可预测的测量值相关联的至少一个区域，其中可以在第二帧平均中包括第二数量的帧。 第二帧数可以小于第一帧数。

    Windowed Sequencing
    18.
    发明申请
    Windowed Sequencing 审中-公开
    窗口排序

    公开(公告)号：US20160168634A1

    公开(公告)日：2016-06-16

    申请号：US15043296

    申请日：2016-02-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Todd REARICK ,  Mark James Milgrew ,  Jonathan Schultz ,  Chris Papalias ,  Kim L. Johnson

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  G06F19/22

    Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors, the output signals indicating chemical reactions occurring proximate to the sensors of the sensor array.

    Abstract translation: 在一个实现中，描述了一种方法。 该方法包括确定传感器阵列的传感器的操作特性。 该方法还包括基于组中的传感器的操作特性来选择阵列中的一组传感器。 该方法还包括使得能够读出所选择的组中的传感器。 所述方法还包括接收来自所述使能的传感器的输出信号，所述输出信号指示在所述传感器阵列的传感器附近发生的化学反应。

    APPARATUSES, METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR FLUID POTENTIAL ARTIFACT CORRECTION IN REAGENT DELIVERY SYSTEMS
    19.
    发明申请
    APPARATUSES, METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR FLUID POTENTIAL ARTIFACT CORRECTION IN REAGENT DELIVERY SYSTEMS 有权
    设备，方法，系统和计算机可读介质，用于在试剂递送系统中进行流体潜在的矫正校正

    公开(公告)号：US20160103093A1

    公开(公告)日：2016-04-14

    申请号：US14853026

    申请日：2015-09-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chiu Tai Andrew WONG ,  Todd REARICK ,  John DONOHUE

    IPC: G01N27/414 ,  G06F19/22 ,  C12Q1/68

    CPC classification number: G01N27/4145 ,  G16B30/00

    Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

    Abstract translation: 用于校正核酸并入信号的方法，用于在核酸序列合成中产生的流体潜在效应或紊乱，包括：将多个模板多核苷酸链设置在设置在传感器阵列上的多个限定的空间中，所述模板多核苷酸链具有测序 引物和与之结合的聚合酶; 将模板多核苷酸链暴露于流过流体歧管的一系列核苷酸物质流，该流体歧管包括用于流动核苷酸物质的通道和用于使溶液流动的分支通道，该分支通道包括参考电极和感测电极; 获得对应于所述多个限定空间的多个核苷酸掺入信号，所述核苷酸掺入信号具有与多个核苷酸结合相关的信号强度; 以及校正所述多个核苷酸并入信号中的至少一些用于流体潜在效应或干扰。

    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS
    20.
    发明申请
    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS 审中-公开

    公开(公告)号：US20160011145A1

    公开(公告)日：2016-01-14

    申请号：US14863718

    申请日：2015-09-24

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan M. ROTHBERG ,  James BUSTILLO ,  Mark James MILGREW ,  Jonathan SCHULTZ ,  David MARRAN ,  Todd REARICK ,  Kim L. JOHNSON

    IPC: G01N27/414 ,  C12Q1/68

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

    Abstract: The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. In one embodiment, the characteristic of said chemical or biological sample is a concentration of a charged species and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating gate with a dielectric layer on a surface thereof, the dielectric layer contacting said sample fluid and being capable of accumulating charge in proportion to a concentration of the charged species in said sample fluid. In one embodiment such charged species is a hydrogen ion such that the sensors measure changes in pH of the sample fluid in or adjacent to the sample-retaining region thereof. Apparatus and chips of the invention may be adapted for large scale pH-based DNA sequencing and other bioscience and biomedical applications.

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