公开(公告)号：US11806714B2

公开(公告)日：2023-11-07

申请号：US17075116

申请日：2020-10-20

Applicant: Massachusetts Institute of Technology

Inventor： Armon R. Sharei ,  Viktor A. Adalsteinsson ,  Nahyun Cho ,  Robert S. Langer ,  J. Christopher Love ,  Klavs F. Jensen

IPC: B01L3/00 ,  C12N5/09 ,  C12N5/078 ,  G01N15/14 ,  C12Q1/04 ,  G01N33/574 ,  G01N1/30 ,  G01N15/10 ,  B82Y30/00 ,  C12M3/06 ,  G01N15/00

CPC classification number: B01L3/502761 ,  C12N5/0634 ,  C12N5/0693 ,  C12Q1/04 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/574 ,  B01L2200/0652 ,  B01L2300/08 ,  B01L2400/0487 ,  B82Y30/00 ,  C12M23/16 ,  G01N1/30 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081

Abstract: Isolating or identifying a cell based on a physical property of said cell can include providing a cell suspension; passing said suspension through a microfluidic channel that includes a constriction; passing the cell suspension through the constriction; and, contacting said cell suspension solution with a compound. The constriction can be sized to preferentially deform a relatively larger cell compared to a relatively smaller cell.

公开(公告)号：US10776608B2

公开(公告)日：2020-09-15

申请号：US16102118

申请日：2018-08-13

Applicant: Massachusetts Institute of Technology

Inventor： Viktor A. Adalsteinsson ,  Denis Loginov ,  J. Christopher Love ,  Alan Stockdale ,  Todd Gierahn

IPC: G06K9/00 ,  B01L3/00 ,  G01N15/14 ,  G01N35/00 ,  G02B21/16 ,  G02B21/26 ,  H04N5/225 ,  G01N33/483 ,  G06K9/03 ,  G06K9/46 ,  G01N15/00 ,  G01N15/10

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

公开(公告)号：US09953209B2

公开(公告)日：2018-04-24

申请号：US15085175

申请日：2016-03-30

Applicant: Massachusetts Institute of Technology

Inventor： Viktor A. Adalsteinsson ,  Denis Loginov ,  J. Christopher Love ,  Alan Stockdale ,  Todd Gierahn

IPC: G06K9/00 ,  B01L3/00 ,  G01N15/14 ,  G01N35/00 ,  G02B21/16 ,  G02B21/26 ,  H04N5/225 ,  G01N33/483 ,  G06K9/03 ,  G06K9/46 ,  G01N15/00 ,  G01N15/10

CPC classification number: G06K9/00147 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0896 ,  B01L2300/12 ,  G01N15/1425 ,  G01N15/1463 ,  G01N33/4833 ,  G01N35/00029 ,  G01N35/00871 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2035/00148 ,  G02B21/16 ,  G02B21/26 ,  G06K9/0014 ,  G06K9/036 ,  G06K9/4647 ,  G06K9/4652 ,  G06K9/4661 ,  H04N5/2256

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

公开(公告)号：US20160217315A1

公开(公告)日：2016-07-28

申请号：US15085175

申请日：2016-03-30

Applicant: Massachusetts Institute of Technology

Inventor： Viktor A. Adalsteinsson ,  Denis Loginov ,  J. Christopher Love ,  Alan Stockdale ,  Todd Gierahn

IPC: G06K9/00 ,  G06K9/46 ,  G06K9/03 ,  G01N33/483

CPC classification number: G06K9/00147 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0896 ,  B01L2300/12 ,  G01N15/1425 ,  G01N15/1463 ,  G01N33/4833 ,  G01N35/00029 ,  G01N35/00871 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2035/00148 ,  G02B21/16 ,  G02B21/26 ,  G06K9/0014 ,  G06K9/036 ,  G06K9/4647 ,  G06K9/4652 ,  G06K9/4661 ,  H04N5/2256

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

公开(公告)号：US20160209319A1

公开(公告)日：2016-07-21

申请号：US14997439

申请日：2016-01-15

Applicant: Massachusetts Institute of Technology

Inventor： Viktor A. Adalsteinsson ,  Denis Loginov ,  J. Christopher Love ,  Alan Stockdale ,  Todd Gierahn

IPC: G01N15/14 ,  G01N35/00 ,  G02B21/16 ,  H04N5/225 ,  G02B21/26 ,  B01L3/00 ,  G06K9/00

CPC classification number: G06K9/00147 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0896 ,  B01L2300/12 ,  G01N15/1425 ,  G01N15/1463 ,  G01N33/4833 ,  G01N35/00029 ,  G01N35/00871 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2035/00148 ,  G02B21/16 ,  G02B21/26 ,  G06K9/0014 ,  G06K9/036 ,  G06K9/4647 ,  G06K9/4652 ,  G06K9/4661 ,  H04N5/2256

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

Abstract translation: 本文描述了用于从生物物质样品（例如生物流体）自动识别和回收感兴趣的个体细胞的系统，方法和装置。 还描述了丰富感兴趣的细胞类型的方法。 这些系统，方法和装置允许以下两个或多个以下协调的性能，例如全部具有相同的装置，从而实现高吞吐量：细胞富集，细胞鉴定和单个细胞恢复用于进一步分析（例如，测序） 的个体回收细胞。