公开(公告)号：US10751386B2

公开(公告)日：2020-08-25

申请号：US15981762

申请日：2018-05-16

Applicant: ModernaTX, Inc.

Inventor： Stephane Bancel ,  Jason P. Schrum ,  Alexander Aristarkhov

IPC: A61K49/00 ,  A61K39/00 ,  A61K39/38 ,  A61K38/17 ,  C07K14/47 ,  A61K45/06 ,  A61K38/46 ,  A61K48/00

Abstract: Provided are compositions and methods for delivering biological moieties such as modified nucleic acids into cells to kill or reduce the growth of microorganisms. Such compositions and methods include the use of modified messenger RNAs, and are useful to treat or prevent microbial infection, or to improve a subject's heath or wellbeing.

公开(公告)号：US10077439B2

公开(公告)日：2018-09-18

申请号：US14777301

申请日：2014-03-13

Applicant: ModernaTX, Inc.

Inventor： William Joseph Issa ,  Yuxun Wang ,  Stephane Bancel

IPC: C12Q1/68 ,  C07H21/00 ,  C12N15/10 ,  C12Q1/6806

CPC classification number: C12N15/1006 ,  C12N15/101 ,  C12Q1/6806 ,  C12Q2521/319 ,  C12Q2565/137

Abstract: The present invention describes methods of removing DNA from an RNA transcript during the mRNA production process. The method embodies procedures for obtaining an in vitro transcription product, and removing any DNA from the product. The DNA can be removed by adding either free DNase or a resin containing immobilized DNase to the product, and recovering the RNA transcript. Alternatively, the DNA template used in the in vitro transcription reaction is labeled. After transcription, the product is applied to a resin that is configured to bind the label, and the RNA transcript is recovered. To detect whether any residual impurities are left in the RNA transcript product, the product is subjected to nuclease digestion and subsequently to liquid chromatography-tandem mass spectrometry analysis to quantitate any residual DNA. The present invention demonstrates efficient and effective methods of isolating an RNA transcript from an in vitro transcription product.

公开(公告)号：US09937233B2

公开(公告)日：2018-04-10

申请号：US15248053

申请日：2016-08-26

Applicant: ModernaTX, Inc.

Inventor： Jason P. Schrum ,  Stephane Bancel ,  Noubar B. Afeyan ,  Kenechi Ejebe

IPC: A61K31/70 ,  C07H21/04 ,  A61K38/19 ,  A61K48/00 ,  A61K31/495 ,  A61K39/395 ,  A01K67/00

CPC classification number: A61K38/193 ,  A61K31/395 ,  A61K31/495 ,  A61K31/7088 ,  A61K31/7115 ,  A61K38/00 ,  A61K38/13 ,  A61K38/195 ,  A61K38/28 ,  A61K39/3955 ,  A61K48/00 ,  A61K48/0066 ,  A61K48/0075 ,  A61K48/0083 ,  C07K14/62 ,  C07K14/685 ,  A61K2300/00

Abstract: Provided are compositions and methods for delivering biological moieties such as modified nucleic acids into cells to modulate protein expression. Such compositions and methods include the use of modified messenger RNAs, and are useful to treat or prevent diseases, disorders or conditions, or to improve a subject's heath or wellbeing.

公开(公告)号：US11845772B2

公开(公告)日：2023-12-19

申请号：US17854187

申请日：2022-06-30

Applicant: ModernaTX, Inc.

Inventor： William Joseph Issa ,  John Grant Aunins ,  Stephane Bancel

IPC: C07H21/02 ,  C12Q1/6806 ,  C12N15/10 ,  C07H1/06 ,  C08B37/00

CPC classification number: C07H21/02 ,  C07H1/06 ,  C08B37/0039 ,  C12N15/1006 ,  C12Q1/6806

Abstract: Disclosed herein are methods for purifying RNA comprising poly A. Also disclosed herein are compositions such as surfaces and oligonucleotides for purifying RNA comprising polyA. Other embodiments are also disclosed. Commercially-available resins having polythymidine oligonucleotide ligands typically contain less than 30 thymidine (2′deoxy) residues and some commercial resin suppliers utilize a distribution of dT chain lengths, not of a discreet length.

公开(公告)号：US10858647B2

公开(公告)日：2020-12-08

申请号：US16049132

申请日：2018-07-30

Applicant: ModernaTX, Inc.

Inventor： William Joseph Issa ,  Yuxun Wang ,  Stephane Bancel

IPC: C12N15/10 ,  C12Q1/6806

Abstract: The present invention describes methods of removing DNA from an RNA transcript during the mRNA production process. The method embodies procedures for obtaining an in vitro transcription product, and removing any DNA from the product. The DNA can be removed by adding either free DNase or a resin containing immobilized DNase to the product, and recovering the RNA transcript. Alternatively, the DNA template used in the in vitro transcription reaction is labeled. After transcription, the product is applied to a resin that is configured to bind the label, and the RNA transcript is recovered. To detect whether any residual impurities are left in the RNA transcript product, the product is subjected to nuclease digestion and subsequently to liquid chromatography-tandem mass spectrometry analysis to quantitate any residual DNA. The present invention demonstrates efficient and effective methods of isolating an RNA transcript from an in vitro transcription product.

公开(公告)号：US10772975B2

公开(公告)日：2020-09-15

申请号：US15790246

申请日：2017-10-23

Applicant: ModernaTX, Inc.

Inventor： Stephane Bancel ,  Tirtha Chakraborty ,  Antonin De Fougerolles ,  Sayda M. Elbashir ,  Matthias John ,  Atanu Roy ,  Susan Whoriskey ,  Kristy M. Wood ,  Paul Hatala ,  Jason P. Schrum ,  Kenechi Ejebe ,  Jeff Lynn Ellsworth ,  Justin Guild

IPC: C07K14/535 ,  A61K38/17 ,  A61K48/00 ,  C12N15/85 ,  C07K14/47 ,  C07K14/62 ,  A61K31/7115 ,  C12N9/88 ,  A61K9/127 ,  C12N15/11 ,  C07K14/705 ,  C07K14/505 ,  C12N9/64 ,  C12N9/10 ,  C12N9/00 ,  C12N9/42 ,  C12N15/87 ,  C12P13/04 ,  A61K38/45 ,  C07K14/005 ,  C12N9/16 ,  C12N9/24 ,  C12N15/52 ,  C07K14/435 ,  C12P21/00 ,  C07K14/485 ,  C07H21/02 ,  C07K14/475 ,  C07K14/495 ,  C07K14/525 ,  C07K14/54 ,  C07K14/56 ,  C07K14/61 ,  C07K14/745 ,  C07K16/28 ,  C07K16/40 ,  C12N9/02 ,  C12N9/12 ,  C12N9/68 ,  A61K47/54 ,  A61K9/14 ,  A61K9/51 ,  A61K38/18 ,  A61K38/19 ,  A61K38/21 ,  A61K38/48 ,  C07K14/515 ,  C07K16/00 ,  A61K31/7088 ,  A61K47/10 ,  A61K38/00

Abstract: The invention relates to a pharmaceutical composition including a plurality of lipid nanoparticles having a mean particle size of between 80 nm and 160 nm and containing an mRNA encoding a polypeptide. The lipid nanoparticles include a cationic lipid, a neutral lipid, a cholesterol, and a PEG lipid. The mRNA contains a 5′-cap, 5′-UTR, a 3′-UTR, and a poly-A region with at least 100 nucleotides.

公开(公告)号：US20180271938A1

公开(公告)日：2018-09-27

申请号：US15981762

申请日：2018-05-16

Applicant: ModernaTX, Inc.

Inventor： Stephane Bancel ,  Jason P. Schrum ,  Alexander Aristarkhov

IPC: A61K38/17 ,  A61K48/00 ,  C07K14/47 ,  A61K45/06 ,  A61K38/46

CPC classification number: A61K38/1729 ,  A61K38/465 ,  A61K45/06 ,  A61K48/0075 ,  C07K14/4723 ,  C12Y301/27

Abstract: Provided are compositions and methods for delivering biological moieties such as modified nucleic acids into cells to kill or reduce the growth of microorganisms. Such compositions and methods include the use of modified messenger RNAs, and are useful to treat or prevent microbial infection, or to improve a subject's heath or wellbeing.

公开(公告)号：US09828416B2

公开(公告)日：2017-11-28

申请号：US14750004

申请日：2015-06-25

Applicant: ModernaTX, Inc.

Inventor： Stephane Bancel ,  Tirtha Chakraborty ,  Antonin de Fougerolles ,  Sayda M. Elbashir ,  Matthias John ,  Atanu Roy ,  Susan Whoriskey ,  Kristy M. Wood ,  Paul Hatala ,  Jason P. Schrum ,  Kenechi Ejebe ,  Jeff Lynn Ellsworth ,  Justin Guild

IPC: C12N15/85 ,  A61K31/7088 ,  A61K47/48 ,  C07K14/535 ,  A61K48/00 ,  C12N15/88 ,  A61K9/127 ,  A61K9/50 ,  C07K19/00 ,  A61K38/18 ,  A61K38/19 ,  A61K38/48 ,  C07K14/47 ,  A61K9/14 ,  A61K38/17 ,  A61K47/10 ,  A61K38/21 ,  A61K38/36 ,  A61K38/44 ,  A61K39/395 ,  C07K14/475 ,  C07K14/505 ,  C07K14/525 ,  C07K14/56 ,  C07K14/565 ,  C07K14/745 ,  C07K14/75 ,  C07K16/28 ,  C07K16/32 ,  C12N9/02 ,  C12N9/64 ,  A61K9/00

CPC classification number: C07K14/535 ,  A61K9/0019 ,  A61K9/1271 ,  A61K9/1272 ,  A61K9/1277 ,  A61K9/14 ,  A61K9/5031 ,  A61K31/7088 ,  A61K38/1767 ,  A61K38/1816 ,  A61K38/1866 ,  A61K38/191 ,  A61K38/193 ,  A61K38/212 ,  A61K38/215 ,  A61K38/36 ,  A61K38/363 ,  A61K38/44 ,  A61K38/4833 ,  A61K38/4846 ,  A61K39/3955 ,  A61K47/10 ,  A61K47/54 ,  A61K47/542 ,  A61K48/00 ,  A61K48/0033 ,  A61K48/0066 ,  A61K48/0075 ,  C07K14/47 ,  C07K14/475 ,  C07K14/505 ,  C07K14/525 ,  C07K14/56 ,  C07K14/565 ,  C07K14/745 ,  C07K14/75 ,  C07K16/2887 ,  C07K16/32 ,  C07K19/00 ,  C12N9/0069 ,  C12N9/644 ,  C12N15/85 ,  C12N15/88 ,  C12N2840/00 ,  C12Y113/12007 ,  C12Y304/21005 ,  C12Y304/21022

Abstract: The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules.

公开(公告)号：US10138507B2

公开(公告)日：2018-11-27

申请号：US14777190

申请日：2014-03-13

Applicant: ModernaTX, Inc.

Inventor： Stephane Bancel ,  William Joseph Issa ,  John Grant Aunins ,  Tirtha Chakraborty

IPC: C12P19/34 ,  C12Q1/68 ,  C12N15/10 ,  C12Q1/6865

Abstract: Described are methods for production of RNA transcripts using a non-amplified, linearized DNA template in an in vitro transcription reaction. Enzymatic 5′ capping and oligo dT purification can also be included in the methods.